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Boston, MA, United States

Vander Heiden J.A.,Yale University | Yaari G.,Bar - Ilan University | Yaari G.,Yale University | Uduman M.,Yale University | And 6 more authors.
Bioinformatics | Year: 2014

Summary: Driven by dramatic technological improvements, large-scale characterization of lymphocyte receptor repertoires via high-throughput sequencing is now feasible. Although promising, the high germline and somatic diversity, especially of B-cell immunoglobulin repertoires, presents challenges for analysis requiring the development of specialized computational pipelines. We developed the REpertoire Sequencing TOolkit (pRESTO) for processing reads from high-throughput lymphocyte receptor studies. pRESTO processes raw sequences to produce error-corrected, sorted and annotated sequence sets, along with a wealth of metrics at each step. The toolkit supports multiplexed primer pools, single- or paired-end reads and emerging technologies that use single-molecule identifiers. pRESTO has been tested on data generated from Roche and Illumina platforms. It has a built-in capacity to parallelize the work between available processors and is able to efficiently process millions of sequences generated by typical high-throughput projects. © The Author 2014.


Patent
Abvitro Inc. | Date: 2014-03-14

Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to heavy and light chain antibody sequences originating from a single cell, antibody discovery, disease and immune diagnostics, and low error sequencing.


Trademark
AbVitro Inc. | Date: 2014-10-09

diagnostic reagents, and immunological substrates and antigens for use in immunological testing and detection of antibodies for medical laboratory or clinical purposes; assays for research, scientific and clinical laboratory use; chemical, biochemical, biological, and biotechnological preparations, namely, antibodies for use in the development or manufacture of immunological reagents and assays for industrial and scientific use; biological preparations, namely, antibodies for laboratories, diagnostics and analytics for scientific or research use. medical assays for testing body fluids for the detection of antibodies; medical diagnostic immunoassays for antibody screening and detection; diagnostic pharmaceutical preparations, namely, antibodies and fragments of antibodies for laboratories, diagnostics, and analytics for human and veterinary medical diagnostic use for use in disease testing; pharmaceutical and veterinary preparations and substances, namely, antibodies and fragments of antibodies for use in disease testing. biochemical research and development.


Patent
AbVitro Inc. | Date: 2014-03-14

Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding for heavy and IgL pairing if antibodies.


Trademark
AbVitro Inc. | Date: 2015-10-07

diagnostic reagents, and immunological substrates and antigens for use in immunological testing and detection of antibodies or antigens for medical laboratory or clinical purposes; assays for research, scientific and clinical laboratory use; chemical, biochemical, biological, and biotechnological preparations, namely, antibodies or antigens for use in the development or manufacture of immunological reagents and assays for industrial and scientific use; biological preparations, namely, antibodies or antigens for laboratories, diagnostics and analytics for scientific or research use. medical assays for testing body fluids for the detection of antibodies or antigens; medical diagnostic immunoassays for antibody or antigen screening and detection; diagnostic pharmaceutical preparations, namely, antibodies, fragments of antibodies, antigens, and fragments of antigens for laboratories, diagnostics, and analytics for human and veterinary medical diagnostic use for use in disease testing; pharmaceutical and veterinary preparations and substances, namely, antibodies, fragments of antibodies, antigens, and fragments of antigens for use in disease testing. biochemical research and development.

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