Time filter

Source Type

Minnetonka, MN, United States

To evaluate the variability of semen analysis, five replicates of 10 different bovine frozen semen batches were coded with different identification numbers and submitted to various laboratories for evaluation. Three studies were conducted: study I included eight laboratories in semen processing centers in the United States; study II included one laboratory in one semen processing center and five veterinary university laboratories in the United States; and study III included five veterinary university laboratories in Brazil. Evaluation methodology, sample classification criteria, and reporting format varied considerably among laboratories. There were laboratory effects (P < 0.05) on sperm concentration, motility, and morphology results in all studies. When Bland-Altman plots were evaluated, differences in sperm concentration were approximately between -5 and +5 × 106 sperm/mL in study I, when the same method of evaluation was used by all laboratories but ranged between -30 and +30 × 106 sperm/mL in studies II and III. Differences in the proportions of motile sperm were approximately -30% to +30%, and differences in the proportion of normal sperm were -15% to +15% in studies I and II; these differences were -15% to +15% and -10% to +10%, respectively, in study III. Mean absolute (one tail) proportional differences in estimates across all laboratories ranged from 9% to 31%, 16% to 37%, and 9% to 14% for sperm concentration, motile sperm, and normal sperm across studies; much larger (48%-86%) differences were observed for sperm abnormality categories. Intralaboratory and interlaboratory precision varied considerably across laboratories and seemed to be at least in part related to methods used for evaluation; precision was better when the NucleoCounter was used for evaluation of sperm concentration, whereas the use of computer-assisted sperm analysis for evaluation of sperm motility resulted in greater precision in some but not all laboratories. None of the laboratories that classified samples as satisfactory or unsatisfactory achieved complete consistency for all replicates within all batches. In addition, consistent classification among laboratories was observed for just three batches in studies II and III. These observations put the reliability of semen analysis in check and make it very difficult, if not impossible, to meaningfully interpret evaluation results. © 2016 Elsevier Inc. Source

Kastelic J.P.,Agriculture and Agri Food Canada | Brito L.F.C.,ABS Inc
Reproduction in Domestic Animals

Contents: Diagnostic ultrasonography has been widely used for examination of the reproductive tract of female cattle, but more sparingly in bulls. Typical clinical ultrasonographic examinations of bull testes are unlikely to affect semen quality or sperm production. The ultrasonographic anatomy of bull testes and accessory sex glands has been reported. Although testicular echogenicity increased (i.e. the parenchyma appeared more white) as a bull approached puberty, echogenicity was not superior to scrotal circumference as a predictor of puberty. Ultrasonography can be used to detect and characterize testicular pathology. It is noteworthy that areas of increased echogenicity (testicular fibrosis) are common, especially in young bulls, but are not associated with decreased semen quality (e.g. percentage of morphologically abnormal sperm). Neither visual evaluation nor computerized pixel analysis of testicular ultrasonic echotexture was consistently predictive of semen quality in bulls. Therefore, we concluded that the primary clinical use of ultrasonography in assessment of reproductive function in the bull is characterization of grossly detectable lesions in the testes and scrotum. © 2012 Blackwell Verlag GmbH. Source

Agency: Department of Energy | Branch: | Program: SBIR | Phase: Phase II | Award Amount: 999.50K | Year: 2014

ABSMaterials, Inc. of Wooster, Ohio has previously developed four important commercial innovations under SBIR development from the US Department of Energy and US National Science Foundation. These previous innovations, cleaning up water from brownfield sites and oil and gas drilling operations, have brought to the companys attention the difficult challenge of treating refinery waters for beneficial reuse. This SBIR IIB will build on the previous technology inventions allowing the company to attempt their innovative technology to energy efficient treatment of complex phenol and sulfur laden waters.

Disclosed is a method and system for applying force against a solid object. A sol-gel derived sorbent material is placed against a solid object to be moved under conditions sufficient to contact the swellable sol-gel derived sorbent material with a sorbate and cause the sol-gel derived sorbent material to swell to at least I times its volume in its unswollen slate to cause sol-gel derived sorbent material to expand and to apply force against the solid object.

Disclosed is a method for removing an ionic species contained in an aqueous phase. The method includes contacting an aqueous phase containing the ionic species with a sol gel-derived composite material, where the sol gel-derived composite material is a sol gel-derived composition having a porous matrix and containing a reactive metal incorporated into at least a portion of the porous matrix, under conditions sufficient to remove the ionic species contained in the aqueous phase.

Discover hidden collaborations