Kikuchi T.,Tokyo Womens Medical University |
Kikuchi T.,CellSeed |
Shimizu T.,Tokyo Womens Medical University |
Wada M.,ABLE Corporation |
And 2 more authors.
Biomaterials | Year: 2014
Automated manufacturing is a key for tissue-engineered therapeutic products to become common-place and economical. Here, we developed an automatic cell sheet stacking apparatus to fabricate 3-dimensional tissue-engineered constructs exploiting our cell sheet manipulator technique, where cell sheets harvested from temperature-responsive culture dishes are stacked into a multilayered cell sheet. By optimizing the stacking conditions and cell seeding conditions, the apparatus was eventually capable of reproducibly making five-layer human skeletal muscle myoblast (HSMM) sheets with a thickness of approximately 70-80μm within 100min. Histological sections and confocal topographies of the five-layer HSMM sheets revealed a stratified structure with no delamination. In cell counts using trypsinization, the live cell numbers in one-, three- and five-layer HSMM sheets were equivalent to the seeded cell numbers at 1h after the stacking processes; however, after subsequent 5-day static cultures, the live cell numbers of the five-layered HSMM sheets decreased slightly, while one- and three-layer HSMM sheets maintained their live cell numbers. This suggests that there are thickness limitations in maintaining tissues in a static culture. We concluded that by combining our cell sheet manipulator technique and industrial robot technology we can create a secure, cost-effective manufacturing system able to produce tissue-engineered products from cell sheets. © 2013 Elsevier Ltd. Source
Li J.,Tongji University |
Liu W.,Tongji University |
Takahashi J.,Tongji University |
Takahashi J.,Obihiro University of Agriculture and Veterinary Medicine |
And 3 more authors.
Chinese Journal of Environmental Engineering | Year: 2016
In this study, Alcaligenes faecalis strain No. 4, which is capable of heterotrophic nitrification and aerobic denitrification, was utilized to directly remove high strength ammonium from the effluent of an anaerobic digester with dewatered sludge. Within 60 h, around 18% of the ammonium (with an initial concentration of 441 mg/L) was removed through direct treatment with Alcaligenes faecalis No. 4.Among the carbon sources contained in the digestate, acetic acid proved to be most suitable. Thus, the lack of carbon sources in the high strength ammonium treatment system could be counteracted by the addition of sodium acetate. When enough sodium acetate was added to ensure the optimal C/N ratio of 10:1, the ammonium removal rate could eventually reach 98%. Therefore, the acidified sludge could be further proposed to be a potential carbon source due to the highly concentrated acetate generated during acidification. © 2016, Science Press. All right reserved. Source
Sekine H.,Tokyo Womens Medical University |
Shimizu T.,Tokyo Womens Medical University |
Sakaguchi K.,Waseda University |
Dobashi I.,Tokyo Womens Medical University |
And 5 more authors.
Nature Communications | Year: 2013
In vitro fabrication of functional vascularized three-dimensional tissues has been a long-standing objective in the field of tissue engineering. Here we report a technique to engineer cardiac tissues with perfusable blood vessels in vitro. Using resected tissue with a connectable artery and vein as a vascular bed, we overlay triple-layer cardiac cell sheets produced from coculture with endothelial cells, and support the tissue construct with media perfused in a bioreactor. We show that endothelial cells connect to capillaries in the vascular bed and form tubular lumens, creating in vitro perfusable blood vessels in the cardiac cell sheets. Thicker engineered tissues can be produced in vitro by overlaying additional triple-layer cell sheets. The vascularized cardiac tissues beat and can be transplanted with blood vessel anastomoses. This technique may create new opportunities for in vitro tissue engineering and has potential therapeutic applications. © 2013 Macmillan Publishers Limited. All rights reserved. Source
Able Inc. | Date: 2014-08-18
Embodiments of the Ergonomic Ladder Rack are comprised of two or more fixed cross-members, one or more inner axis rods, one or more stops, two or more ladder support arms, one or more fluid or gas filled dampers, two or more ladder hooks, two or more ladder stop/securing arms, one or more connecting tubes, and a handle assembly. The cross-members are spaced on the vehicle in such a way as to traverse much of the length of the vehicle. The inner axis rod is placed perpendicular through apertures formed by the fixed cross-members. The connecting tubes extend between each fixed cross-member allow each ladder support arms to rotate in combination with the others. The handle assembly is comprised of a handle, a pair of support braces, a handle axis tube, a bat wing bracket, a rear bushing, a front bushing, and a securing bolt.
Able Corporation | Date: 2011-05-13
The object of the present invention is to provide a technique to inhibit methanogens from producing methane. The use of