Shinjuku, Japan
Shinjuku, Japan

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Sekine H.,Tokyo Women's Medical University | Shimizu T.,Tokyo Women's Medical University | Sakaguchi K.,Waseda University | Dobashi I.,Tokyo Women's Medical University | And 5 more authors.
Nature Communications | Year: 2013

In vitro fabrication of functional vascularized three-dimensional tissues has been a long-standing objective in the field of tissue engineering. Here we report a technique to engineer cardiac tissues with perfusable blood vessels in vitro. Using resected tissue with a connectable artery and vein as a vascular bed, we overlay triple-layer cardiac cell sheets produced from coculture with endothelial cells, and support the tissue construct with media perfused in a bioreactor. We show that endothelial cells connect to capillaries in the vascular bed and form tubular lumens, creating in vitro perfusable blood vessels in the cardiac cell sheets. Thicker engineered tissues can be produced in vitro by overlaying additional triple-layer cell sheets. The vascularized cardiac tissues beat and can be transplanted with blood vessel anastomoses. This technique may create new opportunities for in vitro tissue engineering and has potential therapeutic applications. © 2013 Macmillan Publishers Limited. All rights reserved.


Patent
Able Corporation and Tokyo Women's Medical University | Date: 2015-04-15

Disclosed herein is a cell culture apparatus that can achieve appropriate culture conditions. The cell culture apparatus (1) includes: a cylindrical culture vessel (10) that holds a culture liquid containing cells; a supporting column (20) that stands upright in a center of an inner surface of a bottom (12) in the culture vessel; and a stirring device (30) that includes an attaching portion (32) that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade (34) whose upper portion is fixed to the attaching portion so as to rotate around the supporting column.


Shoda M.,Able Corporation | Ishikawa Y.,Able Corporation
Journal of Bioscience and Bioengineering | Year: 2014

Alcaligenes faecalis strain No. 4 which is capable of heterogeneous nitrification and aerobic denitrification, was used to remove high-strength ammonium (approximately 1g NH4+-N/l) from digested sludge, the product of an anaerobic digestion reactor, in which methane was produced from excess municipal sewage sludge. Repeated batch operations were conducted at 20°C and 30°C for 550h, using a jar fermentor. The removal ratios of high-strength ammonium reached 90-100% within 24h, and the average ammonium removal rate was 2.9kg-N/m3/day, more than 200 times higher than that in conventional nitrification-denitrification processes. During these operations, the cell density was maintained at 108-109 cells of A. faecalis strain No. 4/ml. At 3% NaCl in the digested sludge, strain No. 4 exhibited an ammonium removal rate of 3kg-N/m3/day. © 2013 The Society for Biotechnology, Japan.


Patent
Able Enterprise Co. | Date: 2015-07-23

A motor stator device with simple coil-winding structure is disclosed. The motor stator device includes an insulating base comprising an outer ring base, an inner ring base and a connection base, the outer ring base and the inner ring base alternatively forming outer ring openings, inner ring openings, outer ring sinks and inner ring pillars, the connection base being provided to be wound with a metal coil; a plurality of stator segments being positioned on the insulating base; and a plurality of segment connectors being positioned on the outer ring sinks between the insulating base and the stator segments and having a base part and an extension part opposite to the base part, the extension part being integrally extended from the base part and toward the motor rotor to get close to the inner ring pillar next to the motor rotor, so as to decrease the cogging torque.


Patent
Able Corporation | Date: 2013-01-09

Provided is a combination electrode for ion concentration measurement including a glass electrode and a reference electrode, which is made capable of stably measuring ion concentration by preventing the leakage and entering of a reference electrode internal solution to a portion above a sealing means of a reference electrode chamber, and thereby by securing the insulation between a glass electrode internal electrode and a reference electrode internal electrode. The combination electrode for ion concentration measurement, includes a glass electrode and a reference electrode whose internal electrode is made of silver-silver chloride, wherein: a sealing means configured to seal an upper surface of a reference electrode chamber formed in an outer tube which houses an internal electrode and an internal solution of the reference electrode and around a glass support tube penetrating the outer tube along a substantially center axis thereof includes an annular silver member which the glass support tube penetrates along a center axis thereof and annular sealing elastic members for liquid-tightly sealing a space between the silver member and the glass support tube, and a space between the silver member and the outer tube, respectively; and the reference electrode internal electrode is connected to a terminal for a reference electrode through the silver member.


Patent
Able Corporation | Date: 2011-02-17

A combination electrode for ion concentration measurement is provided including a glass electrode and a reference electrode whose internal electrode is made of silver-silver chloride, wherein a sealing means is provided configured to seal an upper surface of a reference electrode chamber formed in an outer tube which houses an internal electrode and an internal solution of the reference electrode, and around a glass support tube penetrating the outer tube along a substantially center axis thereof an annular silver member which the glass support tube penetrates along a center axis thereof, annular sealing elastic members for liquid-tightly sealing a space between the silver member and the glass support tube, and a space between the silver member and the outer tube, respectively, and the reference electrode internal electrode is connected to a terminal for a reference electrode through the silver member.


Patent
Able Corporation | Date: 2011-05-13

The object of the present invention is to provide a technique to inhibit methanogens from producing methane. The use of Alcaligenes faecalis can inhibit methanogens from producing methane.


Patent
Able Corporation and Hatano | Date: 2013-03-27

The object of the present invention is to provide a technique to inhibit methanogens from producing methane. The use of Alcaligenes faecalis can inhibit methanogens from producing methane.


Patent
Able Inc. | Date: 2014-08-18

Embodiments of the Ergonomic Ladder Rack are comprised of two or more fixed cross-members, one or more inner axis rods, one or more stops, two or more ladder support arms, one or more fluid or gas filled dampers, two or more ladder hooks, two or more ladder stop/securing arms, one or more connecting tubes, and a handle assembly. The cross-members are spaced on the vehicle in such a way as to traverse much of the length of the vehicle. The inner axis rod is placed perpendicular through apertures formed by the fixed cross-members. The connecting tubes extend between each fixed cross-member allow each ladder support arms to rotate in combination with the others. The handle assembly is comprised of a handle, a pair of support braces, a handle axis tube, a bat wing bracket, a rear bushing, a front bushing, and a securing bolt.


Patent
Able Corporation | Date: 2013-11-26

The object of the present invention is to provide a technique to inhibit methanogens from producing methane. The use of Alcaligenes faecalis can inhibit methanogens from producing methane.

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