9th Peoples Hospital of Chongqing

Chongqing, China

9th Peoples Hospital of Chongqing

Chongqing, China

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Fan Y.,Southwest University | Tan X.,9th Peoples Hospital of Chongqing | Ou X.,Southwest University | Chen S.,Southwest University | Wei S.,Southwest University
Biosensors and Bioelectronics | Year: 2017

A sandwich-configuration electrochemiluminescence (ECL) biosensor was constructed for detecting concanavalin A (ConA) based on peroxydisulfate/oxygen (S2O8 2−/O2) system. In this work, the gold nanoflower modified Zn-doped SnO2 was used as a substrate to adsorb recognition element horseradish peroxidase (HRP) for binding ConA. Then, Au nanoparticles-thiosemicarbazide functionalized PtNi nanocubes (AuNPs-TSC-PtNi NCs), as a novel ECL signal tracer, were incubated onto the electrode through a specific carbohydrate-ConA interaction, thus achieving a sandwiched structure. The integration of amplifying effect of both TSC and PtNi NCs on the ECL of S2O8 2−/O2 system endowed the biosensor a high sensitivity. The linear range for ConA detection was from 0.0010 ng/mL to 10 ng/mL with a detection limit of 0.0002 ng/mL (S/N=3). © 2016 Elsevier B.V.


Fan Y.,Southwest University | Tan X.,9th Peoples Hospital of Chongqing | Ou X.,Southwest University | Lu Q.,Southwest University | And 2 more authors.
Electrochimica Acta | Year: 2016

In this work, a novel "on-off" electrochemiluminescence (ECL) biosensor was successfully constructed for ultrasensitive detection of concanavalin A (ConA). The "signal-on" state with strong initial ECL signal was obtained by Ag-doped graphitic carbon nitride nanosheet (Ag-g-C3N4), which was modified onto the glassy carbon electrode (GCE) for immobilizing phenoxy dextran (DexP) through π-π interaction. Then, the target ConA was anchored onto the DexP/Ag-g-C3N4 modified film via the specific carbohydrate-ConA interaction. Successively, polyaniline-3,4,9,10-perylenetetracarboxylic acid-DexP conjugate (denoted as PANI-PTCA-DexP), as the ECL signal quenching probe, was incubated onto the electrode through the carbohydrate-ConA interaction to achieve the "signal-off" state. Here, PTCA was used as a matrix for the high loading of PANI and DexP, PANI served as the quencher towards Ag-g-C3N4 system, and DexP as the recognition element for bounding ConA. With the formation of the sandwiched structure of DexP, ConA and PANI-PTCA-DexP, a desirable quenching ECL signal was measured with S2O8 2- as the co-reactant of Ag-g-C3N4. The quenching effect of PANI towards Ag-g-C3N4 is positively correlated with the concentration of ConA. With such an "on-off" system, the detection of ConA was achieved with a wide linear range from 0.001 ng/mL to 50 ng/mL as well as a low detection limit down to 0.0003 ng/mL. © 2016 Elsevier Ltd. All rights reserved.


Ou X.,Southwest University | Tan X.,9th Peoples Hospital of Chongqing | Wei S.,Southwest University | Chen S.,Southwest University | And 2 more authors.
Analytical Methods | Year: 2014

A sensitive electrochemiluminescence (ECL) biosensor was fabricated for detection of cholesterol based on an anodic ECL of luminol at low potential. First, C60 was functionalized with l-cysteine (l-cys) to obtain an l-cys-C60 composite, which was modified onto the surface of glassy carbon electrodes for adsorbing gold colloidal nanoparticles (AuNPs). Subsequently, cholesterol oxidase (ChOx) was dropped onto the surface of modified electrode to fabricate a cholesterol biosensor. The assembly process was characterized with atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and ECL. Under the optimal conditions, the proposed biosensor exhibited a sensitive response to cholesterol in the concentration range from 1.7 × 10-5 mM to 0.30 mM with the detection limit of 5.7 × 10-6 mM (S/N = 3). Furthermore, the proposed biosensor has good reproducibility, stability and anti-interferent ability. © the Partner Organisations 2014.


Wang C.,Southwest University | Tan X.,9th Peoples Hospital of Chongqing | Chen S.,Southwest University | Yuan R.,Southwest University | And 3 more authors.
Talanta | Year: 2012

A novel scheme for the fabrication of gold/platinum hybrid functionalized ZnO nanorods (Pt-Au@ZnONRs) and multiwalled carbon nanotubes (MWCNTs) modified electrode is presented and its application for cholesterol biosensor is investigated. Firstly, Pt-Au@ZnONRs was prepared by the method of chemical synthesis. Then, the Pt-Au@ZnONRs suspension was dropped on the MWCNTs modified glass carbon electrode, and followed with cholesterol oxidase (ChOx) immobilization by the adsorbing interaction between the nano-material and ChOx as well as the electrostatic interaction between ZnONRs and ChOx molecules. The combination of MWCNTs and Pt-Au@ZnONRs provided a favorable environment for ChOx and resulted in the enhanced analytical response of the biosensor. The resulted biosensor exhibited a linear response to cholesterol in the wide range of 0.1-759.3 μM with a low detection limit of 0.03 μM and a high sensitivity of 26.8 μA mM -1. The calculated apparent Michaelis constant KMapp was 1.84 mM, indicating a high affinity between ChOx and cholesterol. © 2012 Elsevier B.V. All rights reserved.


Chen H.,Southwest University | Tan X.,9th Peoples Hospital of Chongqing | Zhang J.,Southwest University | Lu Q.,Southwest University | And 3 more authors.
RSC Advances | Year: 2014

In this article, a new electrochemiluminescent (ECL) biosensor based on a g-C3N4-hemin nanocomposite and hollow gold nanoparticles (HGNPs) was constructed to detect lactate. Firstly, the g-C3N4 nanosheets were prepared through ultrasonication-assisted liquid exfoliation of bulk g-C3N4, which was obtained through polymerizing melamine under 600°C. Then, the nanocomposites of g-C3N4 nanosheets and hemin were prepared to modify a glassy carbon electrode. Subsequently, HGNPs were self-assembled onto the electrode for adsorbing lactate oxidase to achieve a lactate biosensor. Due to the excellent catalytic effect of g-C3N4-hemin and HGNPs on the luminol/H2O2 ECL system, the as-prepared biosensor exhibited a good response performance to lactate with a linear range of 1.7 × 10-8 to 5.0 × 10-4 M and a detection limit of 5.5 × 10-9 M. In addition, the prepared ECL biosensor exhibited satisfying reproducibility and stability. The g-C3N4-hemin nanocomposite might have great potential application in a luminol/H2O2 ECL system. © The Royal Society of Chemistry 2014.


Ou X.,Southwest University | Tan X.,9th Peoples Hospital of Chongqing | Liu X.,Southwest University | Lu Q.,Southwest University | And 2 more authors.
Biosensors and Bioelectronics | Year: 2015

A novel signal-on electrochemiluminescence (ECL) biosensor for detecting concanavalin A (Con A) was fabricated with phenoxy dextran-graphite-like carbon nitride (DexP-g-C3N4) as signal probe. In this construction strategy, the nanocomposites of three-dimensional graphene and gold nanoparticles (3D-GR-AuNPs) were used as matrix for high loading of glucose oxidase (GOx), which served as recognition element for bounding Con A. Con A further interacted with DexP-g-C3N4 through a specific carbohydrate-Con A interaction to achieve a sandwiched scheme. With the increase of Con A incubated onto the electrode, the ECL signal resulted from DexP-g-C3N4 would enhance, thus achieving a signal-on ECL biosensor for Con A detection. Due to the integration of the virtues of 3D-GR-AuNPs and the excellent ECL performance of DexP-g-C3N4, the prepared biosensor exhibits a wide linear response range from 0.05ng/mL to 100ng/mL and a low detection limit of 17pg/mL (S/N=3). © 2015 Elsevier B.V.


PubMed | 9th Peoples Hospital of Chongqing and Southwest University
Type: | Journal: Biosensors & bioelectronics | Year: 2015

A novel signal-on electrochemiluminescence (ECL) biosensor for detecting concanavalin A (Con A) was fabricated with phenoxy dextran-graphite-like carbon nitride (DexP-g-C3N4) as signal probe. In this construction strategy, the nanocomposites of three-dimensional graphene and gold nanoparticles (3D-GR-AuNPs) were used as matrix for high loading of glucose oxidase (GOx), which served as recognition element for bounding Con A. Con A further interacted with DexP-g-C3N4 through a specific carbohydrate-Con A interaction to achieve a sandwiched scheme. With the increase of Con A incubated onto the electrode, the ECL signal resulted from DexP-g-C3N4 would enhance, thus achieving a signal-on ECL biosensor for Con A detection. Due to the integration of the virtues of 3D-GR-AuNPs and the excellent ECL performance of DexP-g-C3N4, the prepared biosensor exhibits a wide linear response range from 0.05 ng/mL to 100 ng/mL and a low detection limit of 17 pg/mL (S/N=3).


Zhang J.,Southwest University | Chen S.,Southwest University | Tan X.,9th Peoples Hospital of Chongqing | Zhong X.,Southwest University | And 2 more authors.
Biotechnology Letters | Year: 2014

A sensitive electrochemiluminescence (ECL) biosensor for cholesterol detection based on multifunctional core-shell structured microspheres (Fe3O4@SiO2-Au@mpSiO2) is reported. This microsphere consisted of a core of silica-coated magnetite nanoparticle, an active transition layer of gold nanoparticles and a mesoporous silica shell. Scanning electron microscopy was employed to observe the morphology of the nanomaterials and transmission electron microscopy was used to further confirm the subtle structure of Fe3O4@SiO2-Au@mpSiO2. The microspheres possessed a large surface area that increased enzyme loading, and an active transition layer gold nanoparticles enhanced the ECL signal. They were used to immobilize cholesterol oxidase for cholesterol detection with a high sensitivity, low detection limit and wide linear range. The linear range was from 0.83 to 2.62 mM with a detection limit of 0.28 μM (S/N = 3). Moreover, the reproducibility, stability and selectivity of the biosensor were established. © 2014 Springer Science+Business Media Dordrecht.


PubMed | 9th Peoples Hospital of Chongqing and Southwest University
Type: | Journal: Biosensors & bioelectronics | Year: 2016

A sandwich-configuration electrochemiluminescence (ECL) biosensor was constructed for detecting concanavalin A (ConA) based on peroxydisulfate/oxygen (S


PubMed | 9th Peoples Hospital of Chongqing
Type: Journal Article | Journal: Internal medicine journal | Year: 2014

Stroke is often accompanied by hyperglycaemia, and this has an important impact on prognosis. The aim of this study was to investigate the relationship between early hyperglycaemia and the outcome of spontaneous intracerebral haemorrhage (sICH).A systematic literature search on PubMed, Embase, Cochran, WANFANG DATA, VIP and CNKI databases was conducted, and eight eligible studies were retrieved. Relative risks and 95% confidence interval (CI) in the hyperglycaemia group compared with the non-hyperglycaemia group were calculated and meta-analysed when possible.Eight controlled trials and cohort studies totalling 3756 patients addressing early hyperglycaemia and the outcome of sICH were compiled for this meta-analysis. Cut-off points for defining hyperglycaemia was 6.1-8.3mmol/L, and the median cut-off value was 7.5mmol/L. Studies were assigned to one of the two subgroups: the group A (for studies with the values of glucose concentrations above the median cut-off) and the group B (for studies with the values of glucose concentrations below the median cut-off). The RR for short-term death associated with hyperglycaemia was 3.65 (95% confidence interval (CI) (3.08, 4.33); P < 0.0001). In the subgroup analysis, the relative risk values were 3.46 (95% CI (1.66, 7.20); P = 0.0009) and 3.53 (95% CI (2.92, 4.26); P < 0.00001) for the groups A and B respectively. The publication bias showed that Eggers test (P > 0.1), Beggs test (P > 0.05) and Nfs0.05 exceeded included studies.Early hyperglycaemia can significantly increase the rate of early-term death in patients with sICH, independent of the cut-off points for hyperglycaemia.

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