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Zhang Y.,Wuwei Tumor Hospital | Yao Y.,Xuzhou Medical College | Wang H.,Wuwei Tumor Hospital | Guo Y.,Mudanjiang Medical University | And 2 more authors.
Chinese Journal of Cancer Research | Year: 2013

Objective: To test the effects of salidroside on formation and growth of glioma together with tumor microenvironment. Methods: Salidroside extracted from Rhodiola rosea was purified and treated on human glioma cells U251 at the concentration of 20 μg/mL. 3-(4,5-dimethylthiazol-2-yl)-2,5- dephenyltetrazolium bromide (MTT) assay for cytotoxicity and flow cytometry (FCM) for cell cycle analysis were performed. Then for in vivo study, xenotransplantation tumor model in nude mice was generated and treated with salidroside at the concentration of 50 mg/kg·d for totally 20 d. Body weight and tumor size were detected every 2 d after the treatment. The levels of 8-isoprostane, superoxide dismutase (SOD) and malondialdehyde (MDA), special markers for oxidative stress, were detected while immunofluoresence staining was performed for astrocyte detection. Results: For in vitro study, salidroside could decrease the viability of human glioma cells U251 and the growth of U251 cells at G0/G1 checkpoint during the cell cycle. For in vivo study, salidroside could also inhibit the growth of human glioma tissue in nude mice. The body weight of these nude mice treated with salidroside did not decrease as quickly as control group. In the tumor xenotransplantation nude mice model, mice were found of inhibition of oxidative stress by detection of biomarkers. Furthermore, overgrowth of astrocytes due to the stimulation of oxidative stress in the cortex of brain was inhibited after the treatment of salidroside. Conclusions: Salidroside could inhibit the formation and growth of glioma both in vivo and in vitro and improve the tumor microenvironment via inhibition of oxidative stress and astrocytes. © Chinese Journal of Cancer Research. All rights reserved.


Wu X.,Xuzhou Central Hospital | Fei S.-J.,Xuzhou Medical College | Liu J.-Q.,97th Hospital of Chinese PLA | Chen F.-X.,97th Hospital of Chinese PLA | Wu P.,Xuzhou Central Hospital
World Chinese Journal of Digestology | Year: 2010

AIM: To investigate the effects of the culture supernatants of classically activated macrophages (Mφ1) and alternatively activated macrophages (Mφ2) on the proliferation, cytotoxicity, and surface maker expression of gamma delta T (γδT) cells and explore potential mechanisms involved. METHODS: Mφ1 were induced in vitro with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon-γ (IFN-γ), while Mφ2 were induced with macrophage colony-stimulating factor (M-CSF). The isopentenyl pyrophosphate method was used to amplify human peripheral blood γδT cells. The surface markers on macrophages and γδT cells were determined by flow cytometry (FCM). Interleukin-10 (IL-10) and IL-12 levels in the culture supernatants of Mφ1 and Mφ2 were determined by enzyme-linked immunosorbant assay (ELISA) using commercial kits. The proliferation of γδT cells induced with the culture supernatants of Mφ1 and Mφ2 was investigated by methyl thiazoly tetrazolium (MTT) assay. The lactate dehydrogenase (LDH) method was used to detect the cytotoxicity of γδT cells against gastric cancer SGC-7901 cells. RESULTS: After 10 days of induction culture, approximately 73.2% and 61.8% of Mφ1 and Mφ2 highly expressed CD68, respectively. The level of IL-12 secreted by Mφ1 was significantly higher than that secreted by Mφ2 (35 mg/L vs 9 mg/L, P < 0.001). The level of IL-10 secreted by Mφ1 was significantly lower than that secreted by Mφ2 (15 mg/L vs 87 mg/L, P < 0.001). The culture supernatant of Mφ1 could increase the proliferation of γδT cell when compared with those of Mφ2 and control cells (338% vs 11% and 0%, respectively; both P < 0.01). The positive rate of surface maker γδT cell receptor (γδTCR) on γδT cells induced with the culture supernatant of Mφ1 was higher than those on γδT cells induced with the culture supernatants of Mφ2 and control cells (97.3% vs 89.1% and 91.3%, respectively; both P < 0.05). The culture supernatant of Mφ1 could increase the cytotoxicity of γδT cells when compared with those of Mφ2 and control cells (70.18% vs 51.38% and 47.25%, respectively; both P < 0.01). CONCLUSION: The culture supernatant of Mφ1 can increase the proliferation and cytotoxicity of γδT cells, whereas the culture supernatant of Mφ2 has no significant effects.


Chen H.-J.,97th Hospital of Chinese PLA | Zhang A.-S.,Hospital of the 73087 Troop of Chinese PLA | Zhu X.-C.,Xuzhou | Qian Q.-C.,97th Hospital of Chinese PLA | And 5 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Zinc supplementation can accelerate implant-bone fusion. OBJECTIVE: To observe the effect of 60Co irradiation and trace element zinc on implant-bone fusion. METHODS: A total of 36 adult male rabbits were involved. One piece of titanium implant was placed into the proximal end of the rabbit’s bilateral tibial heads respectively to set up the animal model with titanium implants. Then the animals were randomly divided into four groups. Twenty-four hours after implantation, 10 g/L zinc sulfate was administered intramuscularly to the animals in the zinc supplement group at the dose of 4 mg/kg, once per day; 9 g/L normal saline was administered intramuscularly to the animals in the control group at the dose of 4 mg/kg, once per day. The animals in the 60Co irradiation group received 60Co irradiation at 2, 4, 6 days at the dose of 15 Gy per day, and 24 hours after implantation, 9 g/L normal saline was administered intramuscularly at the dose of 4 mg/kg, once per day; while those in the 60Co irradiation and zinc supplement group received 60Co irradiation at 2, 4, 6 days at the dose of 15 Gy per day, and 24 hours after implantation, 10 g/L zinc sulfate was administered intramuscularly to the animals at the dose of 4 mg/kg, once per day. The animals were killed at 1, 4, 12 weeks after treatment. Stereomicroscope was used to observe the histomorphology on the implant-bone interface. RESULTS AND CONCLUSION: Compared to other groups, at the same time, more fibroblasts and fibrous fusion were observed around the implants from the 60Co irradiation group while less bone tissue, especially mature bone tissue, was observed. On the contrary, at the same time, the surfaces of the implants from the zinc supplement group showed more osteoblasts and bone fusion. Mature bone tissue was observed around the implants at the 4th week after implantation indicating that zinc supplement can accelerate the new bone formation on the implant-bone interface at 1-4 weeks after treatment to facilitate the fusion between the implant and bone. The implants from the 60Co irradiation and zinc supplement group showed more bone fusion than those from the 60Co irradiation group at the 4th and 12th weeks, indicating that after 60Co irradiation, zinc supplement still can promote the fusion between the implant and bone. Taken together, these results demonstrate that the appropriate amount of zinc supplemented after irradiation therapy can alleviate the negative effects of irradiation on the implant-bone fusion. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Zhang C.-K.,97th Hospital of Chinese PLA | Shi Y.,97th Hospital of Chinese PLA | Sun Y.,97th Hospital of Chinese PLA | Feng H.,97th Hospital of Chinese PLA | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Bone nonunion due to a variety of causes after fracture is a common orthopedic complication. The existing operation therapy cannot be satisfied with the efficacy. Looking for a minimally invasive, safe and effective treatment method has become the research focus of the orthopedic scholars. OBJECTIVE: To observe the clinical curative effect of autologous bone marrow cell transplantation combined with hyperbaric oxygen in the treatment of nonunion of four limbs. METHODS: Under routine disinfection and local anesthesia, 10-20 mL bone marrow was extracted from patients to separate, condense and prepare bone marrow cell suspension 2-4 mL. The autologous bone marrow cell concentrate was injected to the nonunion site at the multi-punctures under the guidance of positioning of the C-arm X machine. Sterile dressings were performed after local compression for 3-5 minutes. Hyperbaric oxygen therapy was performed after engraftment of bone marrow cells immediately, 10 times as one course. There was an interval of 5 days between the two courses, a total of three courses. RESULTS AND CONCLUSION: Of 37 nonunion cases, 36 cases were healed, and the efficacy assessment was excellent in 25 cases, good in eight cases, general in three cases, and poor in one case; the average time of callus formation was (7.22±1.96) weeks, and the average healing time was (7.91±1.79) months. After bone marrow cell implantation, no serious complications occurred. Autologous bone marrow cell transplantation combined with hyperbaric oxygen for the treatment of bone nonunion can promote the healing of nonunion, which is an effective treatment method.

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