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Zhang Y.,Wuwei Tumor Hospital | Yao Y.,Xuzhou Medical College | Wang H.,Wuwei Tumor Hospital | Guo Y.,Mudanjiang Medical University | And 2 more authors.
Chinese Journal of Cancer Research | Year: 2013

Objective: To test the effects of salidroside on formation and growth of glioma together with tumor microenvironment. Methods: Salidroside extracted from Rhodiola rosea was purified and treated on human glioma cells U251 at the concentration of 20 μg/mL. 3-(4,5-dimethylthiazol-2-yl)-2,5- dephenyltetrazolium bromide (MTT) assay for cytotoxicity and flow cytometry (FCM) for cell cycle analysis were performed. Then for in vivo study, xenotransplantation tumor model in nude mice was generated and treated with salidroside at the concentration of 50 mg/kg·d for totally 20 d. Body weight and tumor size were detected every 2 d after the treatment. The levels of 8-isoprostane, superoxide dismutase (SOD) and malondialdehyde (MDA), special markers for oxidative stress, were detected while immunofluoresence staining was performed for astrocyte detection. Results: For in vitro study, salidroside could decrease the viability of human glioma cells U251 and the growth of U251 cells at G0/G1 checkpoint during the cell cycle. For in vivo study, salidroside could also inhibit the growth of human glioma tissue in nude mice. The body weight of these nude mice treated with salidroside did not decrease as quickly as control group. In the tumor xenotransplantation nude mice model, mice were found of inhibition of oxidative stress by detection of biomarkers. Furthermore, overgrowth of astrocytes due to the stimulation of oxidative stress in the cortex of brain was inhibited after the treatment of salidroside. Conclusions: Salidroside could inhibit the formation and growth of glioma both in vivo and in vitro and improve the tumor microenvironment via inhibition of oxidative stress and astrocytes. © Chinese Journal of Cancer Research. All rights reserved. Source

Zhang C.-K.,97th Hospital of Chinese PLA | Shi Y.,97th Hospital of Chinese PLA | Sun Y.,97th Hospital of Chinese PLA | Feng H.,97th Hospital of Chinese PLA | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Bone nonunion due to a variety of causes after fracture is a common orthopedic complication. The existing operation therapy cannot be satisfied with the efficacy. Looking for a minimally invasive, safe and effective treatment method has become the research focus of the orthopedic scholars. OBJECTIVE: To observe the clinical curative effect of autologous bone marrow cell transplantation combined with hyperbaric oxygen in the treatment of nonunion of four limbs. METHODS: Under routine disinfection and local anesthesia, 10-20 mL bone marrow was extracted from patients to separate, condense and prepare bone marrow cell suspension 2-4 mL. The autologous bone marrow cell concentrate was injected to the nonunion site at the multi-punctures under the guidance of positioning of the C-arm X machine. Sterile dressings were performed after local compression for 3-5 minutes. Hyperbaric oxygen therapy was performed after engraftment of bone marrow cells immediately, 10 times as one course. There was an interval of 5 days between the two courses, a total of three courses. RESULTS AND CONCLUSION: Of 37 nonunion cases, 36 cases were healed, and the efficacy assessment was excellent in 25 cases, good in eight cases, general in three cases, and poor in one case; the average time of callus formation was (7.22±1.96) weeks, and the average healing time was (7.91±1.79) months. After bone marrow cell implantation, no serious complications occurred. Autologous bone marrow cell transplantation combined with hyperbaric oxygen for the treatment of bone nonunion can promote the healing of nonunion, which is an effective treatment method. Source

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