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Qu X.Q.,Orthopaedic Institute of PLA | Wang W.J.,89th Hospital | Tang S.S.,Weifang Medical University | Liu Y.,Orthopaedic Institute of PLA | Wang J.L.,Orthopaedic Institute of PLA
Genetics and Molecular Research | Year: 2015

This study aimed to investigate the expressional profile of interleukin-6 (IL-6) in articular cartilage bone of osteoarthritis (OA) patients and its correlation with OA. A total of 30 articular cartilage bone samples from knee OA patients, which were collected by knee arthroscopy or articular surgery, comprised the study group, and 30 samples of normal articular cartilage tissue comprised the control group. Both mRNA (messenger ribonucleic acid) and protein levels of IL-6 and matrix metalloproteinase-9 (MMP-9) were measured and compared, and a correlation analysis was performed between the two. The integral optical density (IOD) values of MMP-9 and IL-6 proteins in the study group were 9.21 ± 3.22 and 8.94 ± 3.17, respectively; these were significantly higher (P < 0.05) than those in the control group at 3.14 ± 1.48 and 6.64 ± 1.53, respectively. The IOD values of mRNA transcripts for MMP-9 and IL-6 in the study group were 8.31 ± 2.28 and 8.78 ± 3.43, respectively; these were significantly higher than the values in the control group at 3.52 ± 1.37 and 5.21 ± 1.72 (P < 0.05), respectively. Further, the correlation analysis revealed significantly positive relationships for both protein (r = 0.434, P = 0.001) and mRNA (r = 0.413, P = 0.002) levels between MMP-9 and IL-6. In conclusion, articular cartilage tissues in knee OA patients have higher levels of MMP-9 and IL-6 expression, and these may play a synergistic role in OA pathogenesis. © FUNPEC-RP. Source


Yu G.,Changzheng Hospital | Gao Y.,Changzheng Hospital | Ni C.,Changhai Hospital | Chen Y.,Changhai Hospital | And 4 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2011

Aims: EphB2 is a member of the Eph receptor tyrosine kinase family that has been involved in the regulation of cytoskeleton organization and cell migration in various cell types. Its role and regulation in carcinogenesis is controversial, especially in gastric cancer. We detected EphB2 expression and determined its clinical significance and explored its underlying molecular mechanism in gastric cancers. Methods: Tissue microarray blocks containing primary gastric cancer, lymph node metastases, and adjacent normal mucosa specimens obtained from 337 Chinese patients were constructed. Expression of EphB2 in these specimens was analyzed using immunohistochemistry. Mutation analysis at the A9 tract in exon 17 and loss of heterozygosity analysis at the EphB2 gene locus were carried out in 13 sporadic EphB2-negative gastric cancers. Results: Complete loss of EphB2 expression was observed in 177 (52.5%) of the 337 primary tumor and 41 (82%) of the 50 nodal metastases. Loss of EphB2 expression was significantly associated with advanced T stage, nodal metastasis, advanced disease stage, and poor histological differentiation. Loss of EphB2 expression correlated significantly with poor survival rates in both univariate and multivariate analysis. No frameshift mutation, but a higher frequency of allelic loss, was found in EphB2-negative primary and metastatic tumor samples. Conclusions: Frequent deletion and decreased expression of EphB2 protein suggested it as a negative biomarker for gastric carcinogenesis and a potential predictor of the outcome of patients with gastric cancer. © 2010 Springer-Verlag. Source


He M.,Chongqing University | Nian Y.,National University of Defense Technology | Li Y.,National University of Defense Technology | Xiao S.,National University of Defense Technology | Liu Z.,89th Hospital
IEEE Geoscience and Remote Sensing Letters | Year: 2013

A new cross-channel interference suppression method based on the fractional Fourier transform (FRFT) is proposed to eliminate the effect due to cross-channel interference when a full-polarimetric radar adopts the opposite-slope linear frequency modulation transmit signals and de-ramping processing in the simultaneous measurement mode. The FRFT with an appropriate order is operated on the signals in the cross-interference interval. Such order is obtained via the calculation of the frequency-modulation slope of transmitted signals. The cross-channel interference is changed into sinc functions that are detected by a designed threshold and filtered out in the FRFT domain to suppress the cross-channel interference. The simulations show that the proposed method can obviously reduce the peak sidelobe levels and the integrated sidelobe levels of targets in range profiles compared with the traditional method, which is benefit to improve the detection and measurement performances of weak polarimetric scattering components. The proposed method is suitable for stable or slow-moving targets. © 2013 IEEE. Source


Wang W.J.,89th Hospital | Qu X.Q.,Orthopaedic Institute of PLA | Lv W.,89th Hospital | Yu H.Y.,89th Hospital
Genetics and Molecular Research | Year: 2015

We investigated the expression levels of high-mobility group box protein 1 (HMGB-1), CXC chemokine ligand 16 (CXCL16), microRNA (miRNA)-30a and transforming growth factor β1 (TGF-β1) in primary nephritic syndrome (PNS) patients and the clinical significance of this expression. A total of 56 patients with PNS were included in the PNS group, while 50 healthy subjects formed the normal control group. Serum levels of HMGB-1, CXCL16, miRNA-30a, and urinary TGF-β1 concentrations were quantified along with other biochemical indices, including serum albumin, triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein, and urinary proteins. The correlation between levels of HMGB-1, CXCL16, miRNA-30a, and TGF-β1 and biochemical indexes was further analyzed. PNS group patients had significantly higher levels of HMGB-1, CXCL16, miRNA- 30a, and TGF-β1 compared to the control group (P < 0.05). PNS patients also had higher 24-h urinary protein, TG, TC, and LDL levels but lower serum albumin compared to subjects in the control group (P < 0.05). Serum HMGB-1, CXCL16, miRNA-30a, and urinary TGF-β1 levels were all negatively correlated with serum albumin levels, but were positively correlated with TG, TC, LDL, and 24-h urinary protein (P < 0.05 in all cases). Additionally, a positive correlation existed among serum HMGB-1, CXCL16, miRNA-30a, and urinary TGF-β1 levels (P < 0.01). HMGB-1, CXCL16, miRNA-30a, and urinary TGF-β1 were highly expressed in PNS patients and may play important roles in the pathogenesis and development of PNS. © FUNPEC-RP. Source

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