89th Hospital

Weifang, China

89th Hospital

Weifang, China
SEARCH FILTERS
Time filter
Source Type

Qu X.,89th Hospital | Wu J.,Binzhou Peoples Hospital | Li X.,Binzhou Peoples Hospital | Wang J.,89th Hospital
International Journal of Clinical and Experimental Pathology | Year: 2016

Osteoarthritis (OA) is a degenerative joint disease that seriously impairs human health. The main pathological changes are articular cartilage degeneration, joints marginal bone hyperplasia. Advanced OA can cause joint deformities and dysfunction. Pervious research found that IL-6 expression elevated in OA patients, while the role of IL-6 in OA is still unclear. This study aimed to investigate IL-6 mechanism by observing its impact on OA chondrocytes proliferation. 15 cases of cartilage samples were collected from OA patients receiving total joint arthroplasty in XXX hospital. OA cartilage primary cells were treated by type II collagenase and seeded in 96-well plate. CCK8 assay was applied to detect cell proliferation after IL-6 treatment. Real time PCR was used to test miR-200 expression. MiR-200 impact on cartilage proliferation was observed by overexpression and inhibition. Primary chondrocytes grew well after 72 h obtained by type II collagenase. CCK8 assay showed that IL-6 treatment weakened chondrocyte proliferative ability. Real time PCR revealed that miR-200 level decreased to 0.63 times in IL-6 group. Compared with negative control, miR-200 mimic transfection enhanced chondrocyte proliferation, while miR-200 inhibitor transfection weakened chondrocyte proliferative ability. IL-6 inhibits miR-200 expression in OA chondrocytes. IL-6 suppresses chondrocyte proliferation by downregulating miR-200 level.


Yu G.,Changzheng Hospital | Gao Y.,Changzheng Hospital | Ni C.,Changhai Hospital | Chen Y.,Changhai Hospital | And 4 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2011

Aims: EphB2 is a member of the Eph receptor tyrosine kinase family that has been involved in the regulation of cytoskeleton organization and cell migration in various cell types. Its role and regulation in carcinogenesis is controversial, especially in gastric cancer. We detected EphB2 expression and determined its clinical significance and explored its underlying molecular mechanism in gastric cancers. Methods: Tissue microarray blocks containing primary gastric cancer, lymph node metastases, and adjacent normal mucosa specimens obtained from 337 Chinese patients were constructed. Expression of EphB2 in these specimens was analyzed using immunohistochemistry. Mutation analysis at the A9 tract in exon 17 and loss of heterozygosity analysis at the EphB2 gene locus were carried out in 13 sporadic EphB2-negative gastric cancers. Results: Complete loss of EphB2 expression was observed in 177 (52.5%) of the 337 primary tumor and 41 (82%) of the 50 nodal metastases. Loss of EphB2 expression was significantly associated with advanced T stage, nodal metastasis, advanced disease stage, and poor histological differentiation. Loss of EphB2 expression correlated significantly with poor survival rates in both univariate and multivariate analysis. No frameshift mutation, but a higher frequency of allelic loss, was found in EphB2-negative primary and metastatic tumor samples. Conclusions: Frequent deletion and decreased expression of EphB2 protein suggested it as a negative biomarker for gastric carcinogenesis and a potential predictor of the outcome of patients with gastric cancer. © 2010 Springer-Verlag.


Qu X.Q.,89th Hospital | Wang W.J.,89th Hospital | Tang S.S.,Weifang Medical University | Liu Y.,89th Hospital | Wang J.L.,89th Hospital
Genetics and Molecular Research | Year: 2015

This study aimed to investigate the expressional profile of interleukin-6 (IL-6) in articular cartilage bone of osteoarthritis (OA) patients and its correlation with OA. A total of 30 articular cartilage bone samples from knee OA patients, which were collected by knee arthroscopy or articular surgery, comprised the study group, and 30 samples of normal articular cartilage tissue comprised the control group. Both mRNA (messenger ribonucleic acid) and protein levels of IL-6 and matrix metalloproteinase-9 (MMP-9) were measured and compared, and a correlation analysis was performed between the two. The integral optical density (IOD) values of MMP-9 and IL-6 proteins in the study group were 9.21 ± 3.22 and 8.94 ± 3.17, respectively; these were significantly higher (P < 0.05) than those in the control group at 3.14 ± 1.48 and 6.64 ± 1.53, respectively. The IOD values of mRNA transcripts for MMP-9 and IL-6 in the study group were 8.31 ± 2.28 and 8.78 ± 3.43, respectively; these were significantly higher than the values in the control group at 3.52 ± 1.37 and 5.21 ± 1.72 (P < 0.05), respectively. Further, the correlation analysis revealed significantly positive relationships for both protein (r = 0.434, P = 0.001) and mRNA (r = 0.413, P = 0.002) levels between MMP-9 and IL-6. In conclusion, articular cartilage tissues in knee OA patients have higher levels of MMP-9 and IL-6 expression, and these may play a synergistic role in OA pathogenesis. © FUNPEC-RP.


Ma M.J.,Victory Hospital of China Petrochemical Group | Liu H.C.,Weifang Medical University | Qu X.Q.,89th Hospital | Wang J.L.,89th Hospital
Genetics and Molecular Research | Year: 2015

Matrix metalloproteinase-3 (MMP-3) can mediate the occurrence and development of rheumatoid arthritis (RA). The MMP3 promoter gene exhibits polymorphism with 5A/6A alleles. We investigated the correlation between the expression of MMP3 gene polymorphism and RA to provide an objective basis for prognosis evaluation. We enrolled 80 RA patients and 80 healthy subjects. Enzyme-linked immunosorbent assay was used to detect MMP-3 serum levels, pyrosequencing was used to test MMP3 genotypes, and real-time polymerase chain reaction determined MMP-3 mRNA expression levels. Compared with the control group, the serum level of MMP-3 in the RA patients increased significantly (P < 0.05). The serum level of MMP-3 in RA patients in the active period was markedly elevated compared with that in patients in the relief period (P < 0.05). There was no statistically significant difference between MMP3 gene frequency distribution in the RA patients and the control group (P > 0.05). MMP-3 mRNA expression in the RA patients was markedly upregulated compared with the control group (P < 0.05), while RA patients in the active period exhibited higher MMP-3 mRNA expression (P < 0.05). There was no significant difference in MMP-3 mRNA expression between RA patients with or without the 6A/6A genotype (P > 0.05). RA patients exhibited higher serum MMP-3 levels and mRNA expression, which were more obvious in the active period. MMP-3 is associated with the occurrence and development of RA bone erosion, and its serum level and mRNA expression can be treated as important predictors of joint damage. © FUNPEC-RP.


Pan Z.-H.,89th Hospital | Jiang P.-P.,89th Hospital | Xue S.,89th Hospital
Journal of Hand Surgery: European Volume | Year: 2013

A total of eight cases with multiple skin defects of the hand and digits were resurfaced using a free iliac flap. The lesions involved both the hand and multiple digits in five patients and multiple digits in three patients. The average skin flap size was 89.3 cm2. In three, a piece of of vascularized iliac bone was included. There was no flap loss. Flap debulking was performed in five patients at 10-12 weeks post-surgery during the operation for flap separation and inset. Secondary flap debulking was performed in one patient at 6 months post-surgery. The average static 2-point discrimination was 15.4 mm in five patients, whereas the remaining patients only exhibited sensation to pressure. This procedure may require additional refinement; however, the free iliac flap with technical refinements is a viable option for the treatment of multiple skin defects of the hand and digits. © The Author(s) 2013.


Pan Z.-h.,89th Hospital | Jiang P.-p.,89th Hospital | Wang J.-L.,89th Hospital
Journal of Plastic, Reconstructive and Aesthetic Surgery | Year: 2010

Background: The authors present their clinical experience and surgical methods of soft-tissue coverage for the finger using a posterior interosseous free flap. Methods: Twelve posterior interosseous free flaps, including two dual-paddle flaps, were performed in 12 patients. Indications included: 1) patients with soft-tissue coverage of the dorsum of the finger (n = 4), pulp (n = 1), fingertip and pulp (n = 5), 2) patients subsequent to release of finger and palm contracture (n = 1) and 3) patients subsequent to finger separation (n = 1). The posterior interosseous vessels were ligated below the level at which the motor branch to the extensor carpi ulnaris crossed the vessel superficially. The recipient vessels were the proper digital artery and palmar subcutaneous vein (n = 10), deep branch of the ulnar palm artery (n = 1), superficial branch of the radial palm artery (n = 1) and the vena comitante. The cosmetic appearance of the donor and recipient sites, static two-point discrimination and active total range of motion of the operated finger were evaluated in a follow-up visit. Results: All flaps survived completely and all donor sites were closed directly, leaving a linear scar. De-fatting of the flap was carried out in one case in the late postoperative period; all other patients achieved a cosmetically acceptable result. Static two-point discrimination scores averaged 11 mm (range: 8-15 mm). Postoperatively, seven patients maintained normal flexion and extension of the joint. Two patients who had experienced a fracture and flexor injury recovered near-normal flexion and extension after flexor release; the other patients only recovered partially due to severe preoperative joint stiffness. Preoperatively, the active total range of motion of the operated fingers averaged 187 (range: 20-260). The average active total range of motion of the operated fingers at the last follow-up was 210 (range: 60-260). There was a significant difference between the preoperative and follow-up values (p = 0.042). Conclusions: The posterior interosseous free flap may become one valuable option for finger re-surfacing. Avoiding the sacrifice of a main artery of the hand and shorter pedicle can reduce the morbidity and operative time associated with this procedure. In addition, separate regions can be reconstructed with a dual-paddle flap. © 2009 British Association of Plastic, Reconstructive and Aesthetic Surgeons.


PubMed | The Peoples Hospital of Gaomi City and 89th Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

We investigated the expression levels of high-mobility group box protein 1 (HMGB-1), CXC chemokine ligand 16 (CXCL16), microRNA (miRNA)-30a and transforming growth factor 1 (TGF-1) in primary nephritic syndrome (PNS) patients and the clinical significance of this expression. A total of 56 patients with PNS were included in the PNS group, while 50 healthy subjects formed the normal control group. Serum levels of HMGB-1, CXCL16, miRNA-30a, and urinary TGF-1 concentrations were quantified along with other biochemical indices, including serum albumin, triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein, and urinary proteins. The correlation between levels of HMGB-1, CXCL16, miRNA-30a, and TGF-1 and biochemical indexes was further analyzed. PNS group patients had significantly higher levels of HMGB-1, CXCL16, miRNA- 30a, and TGF-1 compared to the control group (P < 0.05). PNS patients also had higher 24-h urinary protein, TG, TC, and LDL levels but lower serum albumin compared to subjects in the control group (P < 0.05). Serum HMGB-1, CXCL16, miRNA-30a, and urinary TGF-1 levels were all negatively correlated with serum albumin levels, but were positively correlated with TG, TC, LDL, and 24-h urinary protein (P < 0.05 in all cases). Additionally, a positive correlation existed among serum HMGB-1, CXCL16, miRNA-30a, and urinary TGF-1 levels (P < 0.01). HMGB-1, CXCL16, miRNA-30a, and urinary TGF-1 were highly expressed in PNS patients and may play important roles in the pathogenesis and development of PNS.


He M.,Chongqing University | Nian Y.,National University of Defense Technology | Li Y.,National University of Defense Technology | Xiao S.,National University of Defense Technology | Liu Z.,89th Hospital
IEEE Geoscience and Remote Sensing Letters | Year: 2013

A new cross-channel interference suppression method based on the fractional Fourier transform (FRFT) is proposed to eliminate the effect due to cross-channel interference when a full-polarimetric radar adopts the opposite-slope linear frequency modulation transmit signals and de-ramping processing in the simultaneous measurement mode. The FRFT with an appropriate order is operated on the signals in the cross-interference interval. Such order is obtained via the calculation of the frequency-modulation slope of transmitted signals. The cross-channel interference is changed into sinc functions that are detected by a designed threshold and filtered out in the FRFT domain to suppress the cross-channel interference. The simulations show that the proposed method can obviously reduce the peak sidelobe levels and the integrated sidelobe levels of targets in range profiles compared with the traditional method, which is benefit to improve the detection and measurement performances of weak polarimetric scattering components. The proposed method is suitable for stable or slow-moving targets. © 2013 IEEE.


PubMed | Victory Hospital of China Petrochemical Group, Weifang Medical University and 89th Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Matrix metalloproteinase-3 (MMP-3) can mediate the occurrence and development of rheumatoid arthritis (RA). The MMP3 promoter gene exhibits polymorphism with 5A/6A alleles. We investigated the correlation between the expression of MMP3 gene polymorphism and RA to provide an objective basis for prognosis evaluation. We enrolled 80 RA patients and 80 healthy subjects. Enzyme-linked immunosorbent assay was used to detect MMP-3 serum levels, pyrosequencing was used to test MMP3 genotypes, and real-time polymerase chain reaction determined MMP-3 mRNA expression levels. Compared with the control group, the serum level of MMP-3 in the RA patients increased significantly (P < 0.05). The serum level of MMP-3 in RA patients in the active period was markedly elevated compared with that in patients in the relief period (P < 0.05). There was no statistically significant difference between MMP3 gene frequency distribution in the RA patients and the control group (P > 0.05). MMP-3 mRNA expression in the RA patients was markedly upregulated compared with the control group (P < 0.05), while RA patients in the active period exhibited higher MMP-3 mRNA expression (P < 0.05). There was no significant difference in MMP-3 mRNA expression between RA patients with or without the 6A/6A genotype (P > 0.05). RA patients exhibited higher serum MMP-3 levels and mRNA expression, which were more obvious in the active period. MMP-3 is associated with the occurrence and development of RA bone erosion, and its serum level and mRNA expression can be treated as important predictors of joint damage.


PubMed | Weifang Medical University and 89th Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

This study aimed to investigate the expressional profile of interleukin-6 (IL-6) in articular cartilage bone of osteoarthritis (OA) patients and its correlation with OA. A total of 30 articular cartilage bone samples from knee OA patients, which were collected by knee arthroscopy or articular surgery, comprised the study group, and 30 samples of normal articular cartilage tissue comprised the control group. Both mRNA (messenger ribonucleic acid) and protein levels of IL-6 and matrix metalloproteinase-9 (MMP-9) were measured and compared, and a correlation analysis was performed between the two. The integral optical density (IOD) values of MMP-9 and IL-6 proteins in the study group were 9.21 3.22 and 8.94 3.17, respectively; these were significantly higher (P < 0.05) than those in the control group at 3.14 1.48 and 6.64 1.53, respectively. The IOD values of mRNA transcripts for MMP-9 and IL-6 in the study group were 8.31 2.28 and 8.78 3.43, respectively; these were significantly higher than the values in the control group at 3.52 1.37 and 5.21 1.72 (P < 0.05), respectively. Further, the correlation analysis revealed significantly positive relationships for both protein (r = 0.434, P = 0.001) and mRNA (r = 0.413, P = 0.002) levels between MMP-9 and IL-6. In conclusion, articular cartilage tissues in knee OA patients have higher levels of MMP-9 and IL-6 expression, and these may play a synergistic role in OA pathogenesis.

Loading 89th Hospital collaborators
Loading 89th Hospital collaborators