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Taian, China

Chen F.,88th Hospital of PLA | Chen G.,Suncun Hospital of Shandong Xinwen Mining Group | Dou Y.,Shandong University | Xu X.,Shanghai University
JRAAS - Journal of the Renin-Angiotensin-Aldosterone System | Year: 2015

Objective: Previous studies on the relationship between angiotensin II type 1 receptor (AT1R) gene (A1166C) polymorphism and breast cancer did not reach the same conclusion. In the present study, we aimed to further evaluate the relationship between the AT1R gene A1166C polymorphism and breast cancer risk. Methods: We selected five case-control studies related to AT1R gene A1166C polymorphism and breast cancer by searching PubMed, EMBase, Chinese Biomedical Literature Database, Chinese CNKI, Web of Science, and the Wanfang database. We utilized Q-test and I2 test to detect the heterogeneity between each study. A random-effects model (I2 > 50%; p < 0.10) or a fixed-effects model (I2 < 50%; p > 0.10) was utilized to merge the odds ratio (OR) and 95% confidence interval (CI) during the meta- analyses. Results: The present study included 972 patients with breast cancer and 1336 cancer-free control subjects. By metaanalysis, we found A1166C polymorphism was associated with decreased risk for breast cancer in Caucasian population in an additive model (C vs. T: OR = 0.77, 95% CI: 0.62-0.96, p = 0.02). However, we did not find associations in other genetic models (AC+CC vs. AA: OR = 0.78, 95% CI: 0.50-1.22, p = 0.28; CC vs. AA+AC: OR = 1.64, 95% CI: 0.94-2.85, p = 0.08). Conclusion: We concluded that AT1R gene A1166C polymorphism was associated with reduced risk for breast cancer. © The Author(s) 2015. Source

Cardiopulmonary resuscitation (CPR) is a sudden emergency procedure that requires a rapid and efficient response, and personnel training in lifesaving procedures. Regular practice and training are necessary to improve resuscitation skills and reduce anxiety among the staff. As one of the most important skills mastered by medical volunteers serving for Mt. Taishan International Mounting Festival, we randomly selected some of them to evaluate the quality of CPR operation and compared the result with that of the untrained doctors and nurses. In order to evaluate the functions of repeating standard CPR training on performance qualities of medical volunteers for Mt. Taishan International Mounting Festival, their performance qualities of CPR were compared with those of the untrained medical workers working in emergency departments of hospitals in Taian. The CPR performance qualities of 52 medical volunteers (Standard Training Group), who had continually taken part in standard CPR technical training for six months, were tested at random and were compared with those of 68 medical workers (Compared Group) working in emergency departments of hospitals in Taian who hadn't attended CPR training within a year. The QCPR 3535 monitor (provided by Philips Company) was used to measure the standard degree of single simulated CPR performance, including the chest compression depth, frequency, released pressure between compressions and performance time of compression and ventilation, the results of which were recorded in the table and the number of practical compression per minute was calculated. The data were analyzed by x2 Test and t Test. The factors which would influence CPR performance, including gender, age, placement, hand skill, posture of compression and frequency of training, were classified and given parameters, and were put to Logistic repression analysis. The CPR performance qualities of volunteers were much higher than those of the compared group. The overall pass rates were respectively 86.4% and 31.9%; the pass rates of medical volunteers in terms of the chest compression depth, frequency, released pressure between compressions were higher than those of the compared group, which were 89.6%, 94.2%, 95.8% vs 50.3%, 53.0%, 83.1%, P<0.01; there were few differences in overall performance time, which were (118.4 ± 13.5s) vs (116.0 ± 10.4s), P>0.05; the duration time of ventilation in each performance section was much shorter than that in the compared group, which were (6.38 ± 1.2) vs (7.47 ± 1.7), P<0.01; there were few differences in the number of practical compression per minute, which were (78.2 ± 3.5) vs (78.8 ± 12.2), P>0.05); the time proportion of compression and ventilation was 2.6:1 vs 2.1:1. The Logistic repression analysis showed that CPR performance qualities were clearly related to hand skill, posture of compression and repeating standard training, which were respectively OR 13.12 and 95%CI (2.35~73.2); OR 30.89, 95%CI (3.62~263.5); OR 4.07,95%CI (1.16~14.2). The CPR performance qualities of volunteers who had had repeating standard training were much higher than those of untrained medical workers, which proved that standard training helped improve CPR performance qualities. Source

Wei H.,Shandong University | Wang Y.,88th Hospital of PLA | Zhang H.,Shandong University | Zhao H.,Shandong University | Jiang W.,Shandong University
RSC Advances | Year: 2016

The sensitive detection of clinically significant DNA is of critical importance in early clinical diagnostics and medical research. Herein, we developed a sensitive fluorescent method for the detection of DNA fragments from the breast cancer 1 gene based on Au nanoparticles (AuNPs) fluorescence switch-mediated target recycling amplification. First, the designed FAM-labeled single-stranded DNA recognition probes at the 5′-terminus (donated as F-DNA) were adsorbed on the surface of AuNPs, followed by the substantial fluorescence quenching of the FAM. Then, the F-DNA specifically hybridized with the complementary region of the target DNA (T-DNA) and desorbed from the AuNPs surface, leading to the recovery of the partially quenched fluorescence. Finally, under the action of Exo III, T-DNA was released and hybridized with F-DNA for the target recycling; thereby large amounts of fluorescent probe fragments were obtained, resulting in significant fluorescent amplification for T-DNA detection. The proposed strategy exhibited a detection limit of 1.0 × 10-11 mol L-1 and good selectivity towards the mismatched T-DNA, which was better than or comparable to the existing nanomaterial-based fluorescent methods. The method possessed perfect recoveries in the human serum and cell lysate. Therefore, the proposed strategy would offer a new potential for quantification of specific DNA sequences in early clinical diagnostics and medical research. © 2016 The Royal Society of Chemistry. Source

In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft mouse model. A luciferase reporter assay revealed that miR-320a could directly bind to the 3′ UTR of BMI-1. Overexpression of BMI-1 rescued miR-320a-mediated biological function. BMI-1 expression was found to be up-regulated and inversely correlated with miR-320a expression in NPC. Collectively, our data indicate that miR-320a plays a tumor suppressor role in the development and progression of NPC and may be a novel therapeutic target against NPC. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Source

Tian X.,PLA Fourth Military Medical University | Zeng G.,PLA Fourth Military Medical University | Li X.,Hospital of Chongming Air Force Field | Wu Z.,88th Hospital of PLA | Wang L.,PLA Fourth Military Medical University
Oncology Reports | Year: 2015

Cantharidin, a type of terpenoid, is a chemical compount secreted by the blister beetle or Mylabris phelarata pallas of the Meloidae family. Cantharidin is known to have good antitumor activity. The present study aimed to investigate the anticancer effect of cantharidin and its possible underlying mechanism using tongue squamous cell carcinoma (TSCC) TCA8113 cells. TCA8113 cells were treated with various concentrations of cantharidin, and the cell viability and cytotoxicity were assessed using MTT and LDH assays, respectively. Flow cytometry was conducted to examine cell apoptosis and colorimetric protease assay was performed to analyze caspase-9/3 activities in TCA8113 cells. qPCR and western blot analysis were used to investigate microRNA-214 (miR-214) expression, as well as the expression of p53, Bcl-2 and Bax proteins in TCA8113 cells. miR-214 and anti-miR-214 were transfected with mimics to examine whether miR-214 expression regulated the anticancer effect of cantharidin on TCA8113 cells and p53, Bcl-2 and Bax protein expression. The anticancer effect of cantharidin significantly inhibited cell proliferation and increased cytotoxicity of TSCC Tca8113 cells in a dose- and time-dependent manner. In addition, cantharidin induced cell apoptosis and activated caspase- 9/3 activities of TSCC Tca8113 cells. Cantharidin markedly weakened miR-214 expression level, activated p53 protein expression, and suppressed the Bcl-2/Bax signaling pathway in Tca8113 cells. Downregulation of miR-214 increased p53 protein expression and decreased the Bcl-2/Bax signaling pathway of TSCC Tca8113 cells. However, the overexpression of miR-214 reduced the anticancer effect of cantharidin on the proliferation and apoptosis of TSCC Tca8113 cells, inhibited p53 protein expression, and increased the Bcl-2/Bax signaling pathway. The results suggested that cantharidin is a potential anticancer drug that can be used to regulate the proliferation and apoptosis of human TSCC Tca8113 cells. Additionally, its mechanism may partially be associated with the downregulation of miR-214, upregulation of p53 protein expression and suppression of the Bcl-2/Bax signaling pathway. Source

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