Zhang Y.-F.,Intensive Care Unit |
Shan L.,88th Hospital of PLA |
An X.-J.,Intensive Care Unit
Acta Pharmacologica Sinica | Year: 2015
To study the effects of tanshinone IIA (TIIA) on lipopolysaccharide (LPS)-induced acute lung injury in mice and the underlying mechanisms.Methods:Mice were injected with LPS (10 mg/kg, ip), then treated with TIIA (10 mg/kg, ip). Seven hours after LPS injection, the lungs were collected for histological study. Protein, LDH, TNF-α and IL-1β levels in bronchoalveolar lavage fluid (BALF) and myeloperoxidase (MPO) activity in lungs were measured. Cell apoptosis and Bcl-2, caspase-3, NF-κB and HIF-1α expression in lungs were assayed.Results:LPS caused marked histological changes in lungs, accompanied by significantly increased lung W/D ratio, protein content and LDH level in BALF, and Evans blue leakage. LPS markedly increased neutrophil infiltration in lungs and inflammatory cytokines in BALF. Furthermore, LPS induced cell apoptosis in lungs, as evidenced by increased TUNEL-positive cells, decreased Bcl-2 content and increased cleaved caspase-3 content. Moreover, LPS significantly increased the expression of NF-αB and HIF-1α in lungs. Treatment of LPS-injected mice with TIIA significantly alleviated these pathological changes in lungs.Conclusion:TIIA alleviates LPS-induced acute lung injury in mice by suppressing inflammatory responses and apoptosis, which is mediated via inhibition of the NF-κB and HIF-1α pathways. © 2015 CPS and SIMM.
Sun L.,88th Hospital of PLA |
Wu B.,88th Hospital of PLA |
Tian M.,88th Hospital of PLA |
Luo Y.,88th Hospital of PLA
Indian Journal of Orthopaedics | Year: 2016
Background: Multiple ligament injured knee is generally described for a scenario when at least 2 of the 4 major ligaments are ruptured. The most effective treatment for these injuries remains controversial. This study presents the clinical outcome of 3 surgical strategies based on personalized treatment. Materials and Methods: Thirty two patients with multiple ligament injured knee were treated by 3 surgical strategies in the acute phase. (1) One-stage: Twelve patients treated by repair and reconstruction of all ruptured ligaments in a single operation. (2) Staged: Eleven patients treated by repair or reconstruction of the extraarticular (EA) ligaments and then intraarticular ligaments in 2nd stage. (3) EA ligament repair: Nine patients underwent only EA ligaments repair. Results: The patients were followed up for an average of 34.7 ± 12.1 months. Significant improvements in knee stabilities (P < 0.01), Lysholm score (P < 0.01) and International Knee Documentation Committee grade (P < 0.01) were noticed in all groups. Of the 32 patients, none had gross mal alignment or gait abnormalities at the latest followup. Comparing the 3 groups, a significant difference in Lysholm score was shown between the one stage group and the EA repair group (P = 0.040); additionally, significant differences were found in 2 subscales of knee injury and osteoarthritis outcome score (P < 0.05). Conclusion: Satisfactory clinical and functional outcomes could be achieved adopting the 3 surgical strategies based on personalized treatment. However, a combination of EA repair and intraarticular repair or reconstruction might be more reasonable options for the young and active patients. © 2016 Indian Journal of Orthopaedics | Published by Wolters Kluwer - Medknow.
Fanshan M.,88th Hospital of PLA
BMC emergency medicine | Year: 2013
Cardiopulmonary resuscitation (CPR) is a sudden emergency procedure that requires a rapid and efficient response, and personnel training in lifesaving procedures. Regular practice and training are necessary to improve resuscitation skills and reduce anxiety among the staff. As one of the most important skills mastered by medical volunteers serving for Mt. Taishan International Mounting Festival, we randomly selected some of them to evaluate the quality of CPR operation and compared the result with that of the untrained doctors and nurses. In order to evaluate the functions of repeating standard CPR training on performance qualities of medical volunteers for Mt. Taishan International Mounting Festival, their performance qualities of CPR were compared with those of the untrained medical workers working in emergency departments of hospitals in Taian. The CPR performance qualities of 52 medical volunteers (Standard Training Group), who had continually taken part in standard CPR technical training for six months, were tested at random and were compared with those of 68 medical workers (Compared Group) working in emergency departments of hospitals in Taian who hadn't attended CPR training within a year. The QCPR 3535 monitor (provided by Philips Company) was used to measure the standard degree of single simulated CPR performance, including the chest compression depth, frequency, released pressure between compressions and performance time of compression and ventilation, the results of which were recorded in the table and the number of practical compression per minute was calculated. The data were analyzed by x2 Test and t Test. The factors which would influence CPR performance, including gender, age, placement, hand skill, posture of compression and frequency of training, were classified and given parameters, and were put to Logistic repression analysis. The CPR performance qualities of volunteers were much higher than those of the compared group. The overall pass rates were respectively 86.4% and 31.9%; the pass rates of medical volunteers in terms of the chest compression depth, frequency, released pressure between compressions were higher than those of the compared group, which were 89.6%, 94.2%, 95.8% vs 50.3%, 53.0%, 83.1%, P<0.01; there were few differences in overall performance time, which were (118.4 ± 13.5s) vs (116.0 ± 10.4s), P>0.05; the duration time of ventilation in each performance section was much shorter than that in the compared group, which were (6.38 ± 1.2) vs (7.47 ± 1.7), P<0.01; there were few differences in the number of practical compression per minute, which were (78.2 ± 3.5) vs (78.8 ± 12.2), P>0.05); the time proportion of compression and ventilation was 2.6:1 vs 2.1:1. The Logistic repression analysis showed that CPR performance qualities were clearly related to hand skill, posture of compression and repeating standard training, which were respectively OR 13.12 and 95%CI (2.35~73.2); OR 30.89, 95%CI (3.62~263.5); OR 4.07,95%CI (1.16~14.2). The CPR performance qualities of volunteers who had had repeating standard training were much higher than those of untrained medical workers, which proved that standard training helped improve CPR performance qualities.
Sun L.,88th Hospital of PLA |
Zhou X.,88th Hospital of PLA |
Wu B.,88th Hospital of PLA |
Tian M.,88th Hospital of PLA
Arthroscopy - Journal of Arthroscopic and Related Surgery | Year: 2012
The purpose of this study was to explore the effects of joint synovial fluid on tendon-to-bone healing in intra-articular ligament reconstruction of the knee. We divided 40 female New Zealand white rabbits into 4 groups randomly, with 10 animals in each group. Transfer of the semitendinosus tendon to the tibial bone tunnel was performed to create tendon-to-bone healing models. An intra-articular bone tunnel (IBT) was used on the left side and an extra-articular bone tunnel (EBT) on the right. Histologic evaluation was performed at 2, 4, and 8 weeks after the operation and biomechanical testing at 8 weeks. On the basis of fibroblast proliferation, collagen fiber density, collagen fiber orientation, and tendon-to-bone connection, histologic scores were significantly lower in the IBT group than in the EBT group at 2, 4, and 8 weeks. Cell counts per high-power field at the tendon-bone interface were significantly lower in the IBT group than in the EBT group at 2 and 4 weeks. In addition, biomechanical testing showed that the IBT group was significantly inferior to the EBT group in terms of ultimate failure load, yield load, and stiffness. There was also a significant difference between the 2 groups in failure mode. Joint synovial fluid appeared to have an inhibitory effect on tendon-to-bone healing in rabbits at an early stage. Our findings imply that prevention of infiltration of joint synovial fluid into the bone tunnel might be beneficial in improving the clinical outcome of cruciate ligament reconstruction of the knee. © 2012 Arthroscopy Association of North America.
Xu T.,Soochow University of China |
Zhang J.,88th Hospital of PLA |
Liu W.,88th Hospital of PLA |
Kong Y.,88th Hospital of PLA |
Zhang Y.,Soochow University of China
Canadian Journal of Neurological Sciences | Year: 2013
Objective: The aim of the study is to explore the association of serum bilirubin levels with admission severity and short term clinical outcomes among acute ischemic stroke patients. Methods: Data were collected from 2361 acute ischemic stroke patients in four hospitals of Shangdong Province during January 2006 and December 2008. National Institutes of Health Stroke Scale (NIHSS) was used to assess admission and discharge severity. NIHSS≥10 at discharge or in-hospital death was defined as short-term clinical outcomes. Logistic regression and trend test were used to examine the association of serum bilirubin levels with admission severity and short term clinical outcomes. Results: Serum bilirubin levels were significantly and positively associated with admission severity (P for trend <0.05). The age-sex adjusted odds ratios (95% confidential intervals) of NIHSS≥10 associated with the second, third and fourth quartile of total bilirubin/direct bilirubin were 1.245 (0.873, 1.777)/1.276 (0.895, 1.818), 1.484 (1.048, 2.102)/1.628 (1.158, 2.289) and 2.869 (2.076, 3.966)/2.765 (1.996, 3.828), respectively, compared with the lowest quartile; the multivariate adjusted odds ratios of NIHSS≥10 associated with the second, third and fourth quartile of total bilirubin/direct bilirubin were 1.088(0.711, 1.665)/1.436(0.94, 2.193), 1.328(0.877, 2.011)/1.647(1.092, 2.485) and 2.336(1.579, 3.458)/3.079 (2.049, 4.623), respectively, compared with the lowest quartile. However, no association between serum bilirubin levels and short-term clinical outcomes was observed in our study. Conclusion: Serum bilirubin levels were associated with initial stroke severity closely. Nevertheless, there is no significant relationship between serum bilirubin levels and short-term clinical outcomes among acute ischemic stroke patients.
Qi X.,88th Hospital of PLA |
Li J.,88th Hospital of PLA |
Zhou C.,88th Hospital of PLA |
Lv C.,88th Hospital of PLA |
Tian M.,88th Hospital of PLA
FEBS Letters | Year: 2014
In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft mouse model. A luciferase reporter assay revealed that miR-320a could directly bind to the 3′ UTR of BMI-1. Overexpression of BMI-1 rescued miR-320a-mediated biological function. BMI-1 expression was found to be up-regulated and inversely correlated with miR-320a expression in NPC. Collectively, our data indicate that miR-320a plays a tumor suppressor role in the development and progression of NPC and may be a novel therapeutic target against NPC. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Cao F.-L.,88th Hospital of PLA |
Xu M.,88th Hospital of PLA |
Wang Y.,PLA Fourth Military Medical University |
Gong K.-R.,University of California at San Francisco |
Zhang J.-T.,88th Hospital of PLA
Pharmacology Biochemistry and Behavior | Year: 2015
Neuropathic pain, characterized by spontaneous pain, hyperalgesia and allodynia, is a devastating neurological disease that seriously affects patients' quality of life. We have previously shown that tanshinone IIA (TIIA), an important lipophilic component of Danshen, had significant anti-nociceptive effect in somatic and visceral pain, it is surprisingly noted that few pharmacological studies have been carried out to explore the possible analgesic action of TIIA on neuropathic pain and the underlying mechanisms. Therefore, in the present study, by using spinal nerve ligation (SNL) pain model, the antinociceptive and antihyperalgesic effects of TIIA on neuropathic pain were evaluated by intraperitoneal administration in rats. The results indicated that TIIA dose-dependently inhibited SNL-induced mechanical hyperalgesia. As revealed by OX42 levels, TIIA effectively repressed the activation of spinal microglial activation in SNL-induced neuropathic pain. Meanwhile, TIIA also decreased the expressions of inflammatory cytokines TNF-α and IL-1β in the spinal cord. Furthermore, TIIA inhibited oxidative stress by significantly rescuing the superoxide dismutase (SOD) activity and decreasing the malondialdehyde (MDA). Moreover, TIIA depressed SNL-induced MAPKs activation in spinal cord. Conclusion Taken together, our study provides evidence that TIIA inhibited SNL-induced neuropathic pain through depressing microglial activation and immune response by the inhibition of mitogen-activated protein kinases (MAPKs) pathways. Our findings suggest that TIIA might be a promising agent in the treatment of neuropathic pain. © 2014 Published by Elsevier Inc.
Wu B.,88th Hospital of PLA |
Zhao Z.,88th Hospital of PLA |
Li S.,88th Hospital of PLA |
Sun L.,88th Hospital of PLA
Arthroscopy - Journal of Arthroscopic and Related Surgery | Year: 2013
Purpose: To determine the effect of remnant preservation on the healing of anterior cruciate ligament (ACL) tendon graft after reconstruction in an animal model. Methods: Fifty-two New Zealand rabbits were divided into 6 groups. Revascularization and graft healing were studied in groups 1 through 4 (n = 8 each). The biomechanical properties of the graft complex were evaluated in group 5 (n = 12). The blood flow and biomechanical characteristics of the intact ACL were evaluated in group 6 (n = 8). Both knees of each rabbit were subjected to ACL reconstruction with 2-mm grafts of Achilles tendon splits. The right knees were reconstructed with remnant preservation, whereas the remnants in the left knees were debrided. The revascularization and remodeling of grafts were evaluated at 6, 12, 18, and 24 weeks after reconstruction. Tendon-to-bone integration was investigated histomorphologically. The tensile load strengths of graft complexes were also tested. Results: The blood flow in the graft was significantly higher in the remnant-preserved group than in the remnant-debrided group (P <.01). Specifically, the blood flow of the graft with remnant preservation was markedly enhanced at 6 weeks and 12 weeks after reconstruction (P <.05). The number of CD34-positive vessels in the graft was higher in the remnant-preservation group (P <.05). The ligamentization and remodeling of the graft were improved and the collagen type III content in the graft was higher in the remnant-preserved group (P <.05). Tendon-to-bone integration was improved by remnant preservation. At 24 weeks after reconstruction, the failure load of the graft complex was significantly higher in the remnant-preserved group (23.46 ± 7.40 N) than in the remnant-debrided group (18.63 ± 4.26 N) (P <.05). Conclusions: The preservation of the remnant attachment in ACL reconstruction improved the revascularization and remodeling of the graft and enhanced the biomechanical properties of the integrated graft. Clinical Relevance: Preservation of the remnant attachment may benefit healing of the tendon graft in ACL reconstruction. © 2013 by the Arthroscopy Association of North America.
Sun L.,88th Hospital of PLA
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2013
To compare the properties and clinical outcomes of arthroscopic reconstruction of anterior cruciate ligament (ACL) with preservation of remnant through outside-in and transtibial tunel. From June 2005 to January 2012, 145 patients were treated with arthroscopic reconstruction of ACL with preservation of remnant. Among the patients, 88 patients were treated with outside-in techniques (outside-in group), including 55 males and 33 females, ranging in age from 18 to 52 years, with a mean of (29.22 +/- 7.31) years; 57 patients were treated with transtibial technique (transtibial group), including 35 males and 22 females, ranging in age from 18 to 51 years, with a mean of (29.28 +/- 8.07) years. The Lysholm, VAS and IKDC scores were compared between two groups before operation, after operation and at the latest follow-up time. The average operation time was (76.94 +/- 10.83) min in the outside-in group, and (70.35 +/- 10.11) min in the transbibial group, there was a significant difference between two groups. There was no significant difference of hydrops articuli scores at the early stage between the two groups (P = 0.065). At follow-up from 18 to 60 months, there were great improvements in the knee stabilities in each group compared with the preoperative data respectively. The Lysholm score improved from 54.75 +/- 10.58 preoperatively to 95.80 +/- 5.16 at the follow-up in the outside-in group; and improved from 52.51 +/- 11.38 preoperatively to 94.86 +/- 5.50 at follow-up in the transtibial group. Additionally, IKDC grades also improved in both groups. However, no significant differences were seen in stabilities shown by pivot shift test, Lachman test and anterior drawer test. And there also no significant differences of Lysholm scores and IKDC grades between two groups after operation. The outside-in technique has advantages to create an anatomical femoral tunnel easily with minimal intra-articular interference, and disadvantages of complicated manipulation. The transtibial technique is easy to operate and gain time. Using either of responding technique according to the actual situation, satisfactory outcome could be archived.
Zheng Z.-F.,Peking Union Medical College |
Zheng Z.-F.,88th Hospital of PLA |
Zhang Q.-J.,Peking Union Medical College |
Chen R.-Y.,Peking Union Medical College |
Yu D.-Q.,Peking Union Medical College
Journal of Asian Natural Products Research | Year: 2012
Four new N-contained iridoid glycosides, lonijapospiroside A (1), l-phenylalaninosecologanin B (2), l-phenylalaninosecologanin C (3), and dehydroprolinoylloganin A (4), were isolated from the flower buds of Lonicera japonica Thunb. Their structures were established on the basis of UV, IR, MS, and NMR spectral data. © 2012 Taylor and Francis Group, LLC.