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Shenyang, China

Qiu J.,Liaoning Medical University | Li W.,General Hospital of Shenyang Military Command | Feng S.,463 Hospital of Chinese PLA | Wang M.,463 Hospital of Chinese PLA | He Z.,Liaoning Medical University
International Journal of Molecular Medicine

The aim of the present study was to investigate the neuroprotective effects of bone marrow-derived endothelial progenitor cell (EPC) transplantation against cerebral ischemia/reperfusion (I/R) injury in rats and to delineate the possible underlying mechanisms. Cerebral I/R injury was established by 2 h of middle cerebral artery occlusion (MCAO) followed by reperfusion for 24 h. EPCs were isolated from bone marrow of the donor rats, grown in conditioned medium, and characterized by flow cytometry analysis of several surface markers. Labeled EPCs (106 cells) were infused into rats at the onset of reperfusion and 12 h after reperfusion via the tail vein. Infarct volume was assessed at 24 h after reperfusion by using triphenyltetrazolium chloride (TTC) staining. The expression of cell apoptosis-related proteins including Bcl-2 and Bax was determined by western blot analysis, and the activity of caspase-3 was also measured. We evaluated the activities of some antioxidative enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), the non-enzymatic scavenger glutathione (GSH) and detected the content of malondialdehyde (MDA) in the ischemic penumbra. Moreover, the expression of nuclear factor-κB (NF-κB) in the ischemic regions of rats was examined by immunohistochemical staining and western blot analysis. The results showed that transplantation of EPCs significantly reduced the cerebral infarct volume, decreased caspase-3 activity, upregulated Bcl-2 expression, and downregulated the expression of Bax and NF-κB. Furthermore, reduced levels of MDA, significantly elevated activities of SOD and GSH as well as GSH-PX were also found in I/R rats transplanted with EPCs. Collectively, our data demonstrated that transplantation of bone marrow-derived EPCs exerts potent neuroprotective functions against cerebral I/R injury in rats, and the protective effects may be associated with its antioxidative and anti-apoptotic properties. Source

Jia J.,Liaoning Medical University | Dai S.,463 Hospital of Chinese PLA | Sun X.,463 Hospital of Chinese PLA | Sang Y.,463 Hospital of Chinese PLA | And 5 more authors.
Molecular Medicine Reports

Human esophageal cancer-related gene 4 (ECRG4) is a potential tumor suppressor gene isolated from human esophageal epithelial cells. Studies have shown that ECRG4 effectively inhibits the proliferation of tumor cells and induces apoptosis. However, the role of ECRG4 in laryngeal cancer has not yet been clearly defined. In this study, a human laryngeal cancer cell line stably overexpressing ECRG4 was established. The effect of ECRG4 on the proliferation and apoptosis of laryngeal cancer cells and the associated mechanisms were investigated. The Hep-2 human laryngeal carcinoma cell line exhibited a low basal level of ECRG4 expression and was selected for the present study. The eukaryotic expression plasmid pcDNA3.1-ECRG4 was constructed and introduced into Hep-2 cells by transfection reagents. Western blot analysis, reverse transcription-quantitative polymerase chain reaction and immunofluorescence staining confirmed high-level expression of ECRG4. The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and colony formation assay showed that ECRG4 overexpression suppressed the proliferative capacity of laryngeal cancer cells in vitro. Cell cycle analysis showed that ECRG4 induced cell cycle arrest at the G0/G1 phase. Flow cytometric analysis and Hoechst staining demonstrated that overexpression of ECRG4 significantly induced apoptosis. Western blot analysis confirmed that Bcl-2-associated X protein, cleaved-caspase-3 and cleaved-poly (ADP-ribose) polymerase were upregulated in the apoptotic process, whereas B-cell lymphoma 2 was downregulated. In conclusion, overexpression of ECRG4 inhibited laryngeal cancer cell proliferation and induced cancer cell apoptosis. Therefore, ECRG4 exhibits potential as an effective target in gene therapy for laryngeal cancer. Source

Fan M.-S.,463 Hospital of Chinese PLA | Yang X.-F.,463 Hospital of Chinese PLA | Wu Y.-X.,463 Hospital of Chinese PLA | Zhang Y.-B.,463 Hospital of Chinese PLA | And 4 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research

BACKGROUND: The transplantation of bone marrow and autologous peripheral blood stem cells into the muscles of lower limbs of diabetes patients with lower limb ischemia can promote blood vessel regeneration, improve and recover the blood flow of affected limbs of diabetes patients with lower limb ischemia. However, the collection has great risk, and requires high levels in patients' age, body condition and psychological reception degree. Compared with bone marrow and autologous peripheral blood stem cells, umbilical cord resource is abundant; cell collection is simple; immunogenicity is weak. OBJECTIVE: To investigate the feasibility that transplantation of human umbilical cord mesenchymal stem cells (HUCMSCs) for treatment of diabetic rabbit of lower limbs extremity arterial. METHODS: Models of diabetic rabbits were established. Femoral arteries of lower limbs were ligated. Prepared HUCMSCs in sign of DIL were directly injected into the left hindlimb four-headed thigh muscles in diabetic rabbit models (cell quantity was 1.67×106 per point). Saline was directly injected into right lower limbs. At 2 and 4 weeks, adductor and gastrocnemius nuscle of the bilateral hindlimbs were collected. Angiogenesis of pathological sections was observed. Capillary density and skin temperature were measured using immunohistochemical staining for CD31. RESULTS AND CONCLUSION: Pathological sections exhibited that collateral vessels were significantly increased in the transplantation group compared with control group. Immunohistochemical staining for CD31 demonstrated that the capillary density was significantly higher in the transplantation group compared with control group. The skin temperature was significantly greater in the transplantation group compared with control group (P ≤ 0.05). Results suggested that HUCMSCs transplantation in the treatment of diabetic rabbit of lower limbs ischemia is an effective measure. Source

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