458 Hospital of PLA

Guangzhou, China

458 Hospital of PLA

Guangzhou, China
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Zheng Z.,Beijing Institute of Biotechnology | Zheng Z.,Changchun University | Li J.,Jiamusi University | Sun J.,Beijing Public Security Hospital | And 11 more authors.
Antiviral Research | Year: 2011

We have suggested recently that ATM-Rad3-Related (ATR) DNA damage signaling pathway, which responds to single-strand breaks in DNA, was activated in response to HBV infection. ATR knockdown cells showed decreased HBV DNA yields, implying HBV infection and replication activate and exploit the activated DNA damage response. Host cell proteins may constitute an attractive target for anti-HBV-1 therapeutics, since development of drug resistance against compounds targeting these cellular cofactor proteins is unlikely. In this study, we show that one of the clinically used compounds of ATR and ataxia telangiectasia-mutated (ATM) kinases inhibitor, theophylline (Tp), significantly reduced the yield of HBV DNA, HBsAg and HBeAg in HepG2215 cell culture system, furthermore, Tp could also suppress serum HBV DNA and HBsAg levels in the HBV-transgenic mice. Consistent with this result, immunohistology also showed reduced intensity of HBsAg staining on livers from Tp-treatment group. Taken together, these data indicated the feasibility of therapeutic approaches that target host cell proteins by inhibiting a cellular gene that was required for HBV replication and provided a potential approach for the prevention and treatment of HBV infection. © 2010.

Sun J.-L.,Southern Medical University | Wen B.,458 Hospital of PLA | Sun H.-T.,Southern Medical University | Chen G.-X.,Southern Medical University | He S.-Q.,Southern Medical University
Chinese Pharmacological Bulletin | Year: 2017

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. Due to the difficulty of diagnosis in the early stage of HCC, most HCCs are diagnosed in intermediate-advanced stage. Moreover, the high invasion, metastasis and recurrence rate of HCC result in the high mortality of HCC. MicroRNAs (miRNAs) are a class of highly conserved, endogenous, small, non-coding, single stranded RNA with the length of 22 nucleotides. There are plentiful of miRNAs in liver. MiRNAs not only can regulate the growth and development of liver, but also are closely related to the formation of HCC. In the process of HCC formation, miRNAs could function as oncogenes or tumor suppressor genes to regulate multiple biological processes related to HCC, including cell differentiation, proliferation, tumorigenesis, angiogenesis, invasion, and metastasis. With the intensive study of molecular mechanisms of miRNAs in the process of HCC formation, increasingly studies have revealed that miRNAs could become sensitive biomarkers and effective therapeutic targets for HCC.

Zhang L.S.,458 Hospital of PLA
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2010

To evaluate the inhibitory effect of recombinant adenovirus carrying human endostatin gene (Ad-endo) on the growth of human pancreatic carcinoma xenograft in nude mice. The expression of endostatin in human pancreatic carcinoma Capan-2 cells was examined by RT-PCR after infection with Ad-endo. The supernatants of Capan-2 cells were collected after 48 h of infection with Ad-endo as the conditioned medium for human umbilical vein endothelial cells (HUVECs), whose proliferation in vitro was assayed. Capan-2 cell xenografts were established to determine the antitumoral effects of Ad-endo in vivo. The intratumoral microvessel density (MVD) was evaluated using CD31 staining. The expression of endostatin gene was detected by PT-PCR in infected Capan-2 cells. The conditioned medium from Ad-endo-infected cells significantly inhibited HUVEC proliferation (P<0.05). Ad-endo significantly suppressed the growth of Capan-2 tumor xenografts in nude mice (P<0.05), and the MVD decreased significantly in the treated tumor (P<0.05) as compared with that in the control group. Adenovirus carrying human endostatin gene produces inhibitory effects on the growth of human pancreatic carcinoma tumors in nude mice.

Wang Z.-Z.,458 Hospital of PLA | Luo X.-R.,458 Hospital of PLA | Ouyang W.-W.,458 Hospital of PLA | Li S.-E.,458 Hospital of PLA | Yang J.,458 Hospital of PLA
Medical Journal of Chinese People's Liberation Army | Year: 2013

Objective To observe the effects of systematic mental intervention, with combined centralized teaching, group interview and individual consulting, on mental health, personality and coping style in recruits, and explore an optimal intervention model for recruits' mental health. Methods Two thousand and sixteen recruits in one unit were involved in the present study, among them 1064 were allocated to study group, and the remaining 952 to control group. Recruits in study group received centralized teaching with battalion as a unit, and received group interview in squad or platoon as a unit, and meanwhile individual interview was conducted. Symptoms Checklist-90 (SCL-90), Eysenck Personality Questionnaire (EPQ) and Simplified Coping Style Questionnaire (SCSQ) were filled one month after they were enlisted in the army and 3 days before the training ended. Recruits in control group undertook the same tests mentioned above only 3 days before the training ended. Results The total score and factor scores except hostility in SCL-90 test were significantly lower after than before systematic mental intervention (P<0.05), the hostility score was slightly higher than before intervention but with no statistical significance (P>0.05). The total score and factor scores except paranoia in SCL-90 test were significantly lower in study group than in control group after intervention (P<0.05). Scores of extraversion-introversion tendency and nervousness were significantly lower after than before intervention (P<0.001), the scores of psychoticism and social lie were slightly higher but with no statistical significance (P>0.05), the score of active coping was significantly higher (P<0.001), and of negative coping was significantly lower (P<0.001) after than before intervention. The ratio of the score over 2 and above declined obviously (P<0.05) in neurosis, SCL-90 abnormality, SCL-90 total scores, number of positive items, somatization, obsession, interpersonal sensitivity, depression, anxiety, hostility, phobia, paranoid, and psychotic factor after than before intervention in recruits. Conclusion Systematic mental intervention, which consisted of combined centralized teaching, group interview and individual consulting, may promote the mental health, personality and coping style in recruits.

Chen X.P.,458 Hospital of PLA
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2010

To study the clinical effect of segmental resection of the liver using Glissonean pedicle transection for primary liver cancer. The clinical data of 55 primary liver cancer patients admitted from January 2006 to October 2008 were analyzed retrospectively. Twenty-five of the patients underwent segmental resection of the liver by Glissonean pedicle transection (group A), and 30 underwent routine hepatectomy (group B). The positivity rate of the resection margin, micrometastasis in the hepatic parenchyma surrounding the lesions and postoperative recurrence rates were investigated. The positivity rate of the resection margin was 4.0% in group A, significantly lower than that of group B. The number of histological micrometastasis was significantly higher in group A than in group B (16 vs 8). The median distance of histological micrometastasis was 6.8 mm (2.7-25.6 mm) in group A and 4.2 mm (2.4-9.0 mm) in group B. The one-year recurrence rate was significantly lower in group A than in group B (16% vs 26.7%). Glissonean pedicle transection for segmental liver resection is a simpler procedure than routine hepatectomy for primary liver cancer and can reduce the number of histological micrometastasis and recurrence rate.

Li X.,458 Hospital of PLA | Liu G.,458 Hospital of PLA | Chen M.,458 Hospital of PLA | Yang Y.,458 Hospital of PLA | And 2 more authors.
Hepatitis Monthly | Year: 2016

Background: Absence of an immunocompetent mouse model of persistent hepatitis B virus (HBV) infection has hindered the research of HBV infection and the development of antiviral medications. Objectives: In the present study, we aimed to develop a novel HBV genotype C mouse model by hydrodynamic injection (HI) and then used it to evaluate the antiviral activity of lamivudine. Materials and Methods: A quantity of 15 μg of HBV plasmid [pcDNA3.1 (+)-HBV1.3C], adeno-associated virus-HBV1.3C (pAAV-HBV1.3C) or pAAV-HBV1.2A) were injected into male C57BL/6 mice, by HI, accounting for a total of 13 mice per group. Then, lamivudine was administered to mice with sustained HBV viremia, for 4 weeks. Real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry methods were used to detect HBsAg, HBeAg, HBsAb, HBcAg and HBV DNA, in serum or liver of the mice, at indicated time points. Results: In 60% of the mice injected with pcDNA3.1 (+)-HBV1.3C, HBsAg, HBeAg, HBcAg and HBV DNA persisted for > 20 weeks in liver, post-injection, with no HBsAb appearance. Meanwhile, no significant inflammation was observed in these mice. Compared with pAAV-HBV1.2A and pAAV-HBV1.3C, pcDNA3.1 (+)-HBV1.3C administration led to higher and longer HBV viremia. Furthermore, serum HBV DNA was significantly reduced by lamivudine, after 4 weeks administration, and returned to the original level, after ceasing administration for 1 week, in the mice. Conclusions: In conclusion, our observations indicated that pcDNA3.1 (+)-HBV1.3C was superior to AAV/HBV plasmid for establishment of persistent HBV infection by HI, in vivo, and this mouse model could be useful for studies of hepatitis virology and for the development of innovatory treatments for HBV infections. © 2016, Kowsar Corp.

Min N.,458 Hospital of PLA
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2010

To evaluate the efficacy and toxicity of the combined therapy with oxaliplatin and capecitabine (XELOX) in patients with advanced or recurrent gastric cancer. Forty-one patients with previously untreated advanced or recurrent gastric cancer received intravenous infusion of oxaliplatin at the dose of 130 mg/m(2) on day 1 and oral administration of capecitabine at 1000 mg/m(2) twice a day on days 1-14. The chemotherapy was repeated every 2 weeks for a median of 4 cycles. Two of 41 patients achieved a complete response, and 15 had partial responses, with an overall response rate of 41.5%. Stable disease was observed in 11 patients and progressive disease in 9. The median time to progression and overall survival was 6.2 months and 11.8 months. All the 41 patients were evaluated for toxicity according to NCI criteria, 4 showed grade 3-4 neural toxicity, 4 had hematological toxicity and 3 had hand-foot syndrome. The XELOX regimen shows good efficacy with an acceptable toxicity profile in advanced or recurrent gastric cancer patient.

Chen F.N.,458 Hospital of PLA
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2011

To summarize multi-detector row CT (MDCT) and magnetic resonance imaging (MRI) features of retinal detachment and evaluate the diagnostic value of these two imaging modalities. The MDCT and MRI manifestations were reviewed in 45 cases (47 eyes) of retinal detachment, among which 16 cases (17 eyes) were examined by MDCT and 29 cases (30 eyes) by MRI. Thirty-two cases (33 eyes) were confirmed by operation, and the other 13 cases (14 eyes) were confirmed based on the clinical findings. MDCT and MRI displayed signs of fluid retention between the detached retina and the posterior wall of the eyeball in the cases. Among all these cases, 21 eyes showed simple retinal detachment and 26 had also other pathologies (hemorrhage in 20 eyes and calcification in 6 eyes). Choroidal osteoma was identified in 3 eyes and melanoma of choroid in 5 eyes. MDCT is sensitive in detecting calcification in the eyes and MRI with a minimal risk of radiation, and shows advantages in displaying hemorrhage and confined retinal detachment. Both MDCT and MR have high clinical value in the diagnosis of retinal detachment, and their choice depends on the individual condition of the patients.

PubMed | 458 Hospital of PLA
Type: Journal Article | Journal: World journal of gastroenterology | Year: 2017

To assess the efficacy and safety of Two hundred and twenty-five patients were randomized to receive either LAM + vaccine (vaccine group, In the modified intent-to-treat population, more patients had a decrease in HBV DNA > 2 logThe primary endpoint was not achieved using the HBV DNA vaccine. The HBV DNA vaccine could only be beneficial in subjects that have achieved initial virological response under LAM chemotherapy.

PubMed | Chinese Institute of Aviation Medicine, 458 Hospital of PLA and 302 Hospital of PLA
Type: | Journal: Journal of ethnopharmacology | Year: 2016

Water dropwort [Oenanthe javanica (O. javanica)] is an aquatic perennial herb cultivated in East Asian countries. It has been popularly used in traditional Chinese medicine which is beneficial for the treatment of many diseases, including jaundice and various types of chronic and acute hepatitis. In the present study, we investigated the hepatoprotective effect of total phenolics from O. javanica (TPOJ) against D-galactosamine (D-GalN) induced liver injury in mice.The hepatoprotective activity of TPOJ (125, 250 and 500mg/kg) was investigated on D-GalN (800mg/kg)-induced liver damages in mice. Blood and liver were collected for biochemical and microscopic analysis. RT-PCR was used to determine the changes in hepatic nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Protein levels of iNOS, COX-2, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were determined by western blotting.In the animal studies, TPOJ could improve the survival of acute liver failure model significantly and prevente the D-GalN-induced elevation of the serum enzymatic markers and nonenzymatic markers levels significantly. Meanwhile, TPOJ-treatment decreased the malondialdehyde (MDA) level and elevated the content of glutathione (GSH) in the liver as compared to those in the D-GalN group. Hepatic activities and protein expressions of antioxidative enzymes, including SOD, GPx, and CAT were enhanced dose dependently with TPOJ. At the same time, application of TPOJ effectively suppressed the D-GalN-induced proinflammatory mRNA and protein expression of iNOS and COX-2. Subsequently, the serum levels of proinflammatory mediators, nitric oxide (NO) and prostaglandin E2 (PGE2) were reduced. Additionally, histological analyses also showed that TPOJ reduced the extent of liver lesions induced by D-GalN.Our investigation demonstrated the hepatoprotective activity of TPOJ and revealed that TPOJ attributed its significance in the traditional use for treating liver diseases.

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