455th Hospital of PLA

China

455th Hospital of PLA

China

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Zhang W.-J.,Shanghai University | Li Y.-F.,455th Hospital of PLA | Zhang J.-L.,Shanghai University | Xu M.,455th Hospital of PLA | And 2 more authors.
Annals of Plastic Surgery | Year: 2013

BACKGROUND: The number of citations of an article in scientific journals reflects its impact on a biomedical specialty and its recognition in the scientific community. In this study, we identified and analyzed the 100 most highly cited articles published between 1971 and 2011 in journals pertaining to plastic and reconstructive surgery research. METHODS: We selected 6 high-impact journals for literature search in the database of the Science Citation Index (1971 to 2011). We identified the 100 most frequently cited articles published in these journals, and analyzed them. RESULTS: The most cited article received 1007 citations and the least cited article received 165 citations, with a mean of 274 citations per article. These citation classics were published in 5 high-impact journals, led by Plastic and Reconstructive Surgery (76 articles). The articles came from 17 countries, with the United States producing 57 articles. Sixty-two institutions produced these 100 top-cited articles, led by Royal Mountain Hospital, University of Melbourne (7 articles). Twenty-three authors published 2 or more of the top-cited articles, led by Taylor GI who authored 10 classic papers. CONCLUSIONS: This analysis of the top citation classics allows for the recognition of major advances and supplies a historic perspective on the progress of plastic and reconstructive surgery research. Copyright © 2013 by Lippincott Williams & Wilkins.


Lu J.-R.,7th Peoples Hospital | Yi Y.,Fudan University | Tian J.,455th Hospital of PLA | Xiong C.-X.,7th Peoples Hospital | And 3 more authors.
Blood Purification | Year: 2014

Background: The goal of this study was to investigate underlying factors of parathyroid dysfunction in elderly patients undergoing maintenance hemodialysis. Methods: A total of 286 patients on maintenance hemodialysis were included. Hemoglobin, serum creatinine (Scr), blood urea nitrogen (BUN), serum calcium, serum phosphorus (P), intact parathyroid hormone (iPTH), and serum albumin (Alb) were measured and analyzed both before and after dialysis. Results: A higher incidence of low iPTH level (<150 pg/l) was observed in the elderly group than that in the non-elderly group (55.8 vs. 36.7%, p < 0.05). Elderly patients had a shorter dialysis duration, lighter dry weight, lower concentrations of BUN, Scr, P, iPTH, Alb and standard protein nitrogen present rate (nPNA) compared to that of non-elderly group patients (p < 0.05). Conclusions: Low iPTH level occurs more frequently in elderly hemodialysis patients. Furthermore, age, serum P, serum Alb and nPNA were independently associated with a low iPTH level. © 2014 S. Karger AG, Basel.


Yi Y.,Fudan University | Lu J.-R.,7th Peoples Hospital | Tian J.,455th Hospital of PLA | Ma J.,Fudan University | And 2 more authors.
Experimental and Clinical Cardiology | Year: 2014

Objective: We aimed to evaluate the treatment effect of continuous venovenous hemofiltration (CVVH) in maintenance hemodialysis (MHD) patients with refractory hypertension (RH). Methods:A total of 56 MHD patients with RH were selected to CVVH group. The changing of blood pressure, dry body weight, levels of intact parathyroid hormone (iPTH), renin (RA), angiotensin I, II (AT I, AT II), aldosterone (Ald) in plasma were observed before and after CVVH. Another 58 MHD patients with normal blood pressure were selected as control group. Results:1. 56 MHD patients with RH undergone 3-5 times (average 3.8 times) CCVH treatment. 36 cases were significant effciency, 12 cases were effciency, 8 cases were invalid .The significant effective is 64.3%,effective rate is 85.7%. 2. Mean arterial pressure (MAP) decreased from 137.8±6.3 to 104.2±6.8 mmHg, and the average dry body weight (DBW) decreased from 60.6±8.1 to 58.1±7.6 Kg in CVVH group. 3. The plasma levels of RA ATI, ATII, Ald and iPTH after treatment decreased significantly compared with those before treatment in CVVH group, P <0.05; while there was no significant change in the level of RA, ATI, ATII, Ald and iPTH between before and after treatment in control group. Conclusion: CVVH is effective methods in treatment of MHD patients with RH, and its antihypertensive mechanisms was associated with remove of the excess water and sodium and the decrease in plasma levels of RA, AT I, ATII, Ald, iPTH.


Liu N.,455th Hospital of PLA | Tian J.,455th Hospital of PLA | Cheng J.,455th Hospital of PLA | Zhang J.,455th Hospital of PLA
Journal of Cellular Biochemistry | Year: 2013

Bone marrow-derived mesenchymal stem cells (BMSCs) can migrate to the injured kidney after acute kidney injury (AKI) with limited efficiency. This study investigated the effect of CXCR4 overexpression on BMSC migration to the AKI kidney and the possible mechanisms. CXCR4 gene-modified BMSCs (CXCR4-BMSCs) and null-BMSCs were prepared and transplanted into the AKI mice. Blood indicators, histology, expression of stromal cell-derived factor 1 (SDF-1), and BMSC migration were investigated. Hypoxia/re-oxygenation-pretreated renal tubular epithelial cells (HR-RTECs) were prepared to generate AKI in vitro. The chemotaxis experiment was performed using the transwell chamber. The phosphorylation of AKT and MAPK in the BMSCs was also investigated. The CXCR4-BMSCs showed a remarkable expression of CXCR4. The SDF-1 expression in the AKI renal tissue was increased. CXCR4-BMSCs transplantation sharply increased the accumulation of BMSCs in the renal tissue, which was consistent with a greater improvement of renal function. The in vitro experiments showed that the migration of BMSCs to the HR-RTEC culturing chamber was CXCR4-dependent, and could be fully inhibited by AMD3100, a CXCR4-specific antagonist. The migration could also be partly blocked by either LY294002 (PI3K inhibitor) or PD98059 (MAPK inhibitor). Phosphorylated Akt and MAPK were increased in the BMSCs co-cultured with HR-RTECs and their expression was the highest in the CXCR4-BMSCs, which could be recovered by AMD3100. Overexpression of CXCR4 gene could enhance BMSC migration to the kidney area after AKI. The SDF-1/CXCR4 axis via its activation of PI3K/AKT and MAPK in BMSCs could be the possible mechanisms underlying this function. © 2013 Wiley Periodicals, Inc.


Li H.,455th Hospital of PLA
Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation | Year: 2012

The codes of medical consumptive materials is the base for standardized and information management. This article analyzes in detail the structure and meanings of five common-used classification codes in management including registration certificate code, bar code of high-value medical consumptive materials, charging code, medical insurance code and account code. The disadvantages of current coding method and solving program are suggested.


Liu N.,455th hospital of PLA | Han G.,455th hospital of PLA | Cheng J.,455th hospital of PLA | Huang J.,455th hospital of PLA | Tian J.,455th hospital of PLA
Experimental Biology and Medicine | Year: 2013

Bone-marrow mesenchymal stem cells (BMSCs) transplantation is effective for acute kidney injury (AKI) repair but with limited efficiency. In the present study, BMSCs marked by bromodeoxyuridine (BrdU) were transplanted to the AKI mouse model with erythropoietin (EPO) being subcutaneously injected. The blood urea nitrogen (BUN) and serum creatinine (Scr) levels, pathological changes, distribution of BMSCs, expressions of the cytokeratin 18 (CK18) and the stromal cell-derived factor 1 (SDF-1) in the nephridial tissues were measured. The directional migration of BMSCs to the AKI microenvironment in vitro was also tested. The results showed that BMSCs transplantation or EPO injection alone decreased the BUN and Scr levels and the acute tubular necrosis (ATN) scoring in varied degrees. The combination of these decreased the above indicators' levels significantly. BrdU+ cells (BMSCs) were observed in the AKI nephridial tissues, and CK18 expressed in the cytoplasm of these cells. EPO injection increased the proportion of BrdU+ cells with the enhanced expression of SDF-1 in the AKI nephridial tissues. EPO increased the migrating number of BMSCs to the AKI microenvironment in vitro, and additional anti-SDF-1 treatment with SDF-1 antibody neutralized this effect. Our results showed that EPO increased the number of the transplanted BMSCs in the injured nephridial tissues and enhanced the AKI repair effect of BMSCs transplantation. The enhanced kidney-homing efficiency for BMSCs mediated by the SDF-1/CXCR4 pathway is one of the possible mechanisms for EPO performance. © 2013 by the Society for Experimental Biology and Medicine.


Liu N.,455th Hospital of PLA | Wang H.,455th Hospital of PLA | Han G.,455th Hospital of PLA | Tian J.,455th Hospital of PLA | And 2 more authors.
International Journal of Biochemistry and Cell Biology | Year: 2015

Bone marrow-derived mesenchymal stem cells (BMSCs) transplantation is beneficial for the treatment of acute kidney injury (AKI), but the poor survival of BMSCs limits the repair effect. The oxidative stress in the AKI microenvironment is regarded as the main reason. Considering the potent anti-oxidant ability of heme oxygenase-1 (HO-1), HO-1 overexpression in BMSCs can be expected to improve the survival of BMSCs and correspondingly enhance the AKI repair effect. Here, BMSCs are transfected with pLV-HO-1/eGFP and pLV-eGFP by the lentivirus vector to get HO-1-BMSCs and eGFP-BMSCs, respectively. Ischemia/reperfusion-AKI kidney homogenate supernatant (KHS) is prepared for treating BMSCs, eGFP-BMSCs and HO-1-BMSCs. AKI-KHS results in a high inhibitory rate of BMSCs growth and a high proportion of TUNEL positive BMSCs, while HO-1 overexpression inverses this phenomenon and re-establishes the antioxidant and oxidant balance in HO-1-BMSCs. Phosphorylations of p53 and p38 mitogen-activated protein kinases (p38 MAPK) in HO-1-BMSCs decrease. Lower levels of monocyte chemotactic protein 1, tumor necrosis factor-α and interleukin 1β are also observed in supernatant of HO-1-BMSCs. The in vivo study shows that HO-1 overexpression sharply decreases the apoptosis of BMSCs in the injured kidney, and correspondingly the renal function of the AKI rats improves significantly. In conclusion, BMSCs with HO-1 overexpression suggests a better survival in the I/R-AKI microenvironment and a better kidney repair effect. The anti-oxidant effect via the inactivations of the downstream p53 and p38MAPK in BMSCs and the anti-inflammation could be the mechanisms. It provides a novel approach for the cell-based AKI-therapy. © 2015 Elsevier Ltd. All rights reserved.


PubMed | 455th Hospital of PLA
Type: Journal Article | Journal: Experimental biology and medicine (Maywood, N.J.) | Year: 2011

Erythropoietin (EPO) can stimulate the proliferation and protraction of endothelial progenitor cells, and plays an important role in the proliferation and differentiation of marrow-derived mesenchymal stem cells (mMSCs) under the acute kidney injury (AKI) microenvironment. In the present study, C57BL/6 mice mMSCs were isolated, and AKI mice models were prepared. The renal cortex was obtained to prepare the ischemia/reperfusion (I/R) kidney homogenate supernatant. P3-mMSCs were treated by different methods: one group was added only I/R kidney homogenate supernatant, and another contained different concentrations of EPO (1, 5, 10, 50 IU/mL) in I/R kidney homogenate supernatant. The proliferation and apoptosis of mMSCs were detected by CCK-8 and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling), respectively. Expression of erythropoietin receptor (EPOR) and protein of the signal pathway related to proliferation/apoptosis were also examined. The results showed that the proliferation ability of mMSCs treated with I/R kidney homogenate supernatant decreased significantly, while the apoptosis percentage was significantly higher than that of the control. After intervention of EPO, their proliferation enhanced and the apoptosis percentage decreased. EPOR expression was positive in P3-mMSCs. EPO decreased the expression of caspase-3 of mMSCs under the AKI microenvironment in a dose- and time-dependent manner, but increased the Bcl-2 expression. The expression of phosphor-Janus kinase 2, phosphor-signal transducer and activator of transcription (pSTAT-5) increased significantly in 10 IU/mL EPO cultured for five days. Our results show that EPO can promote proliferation of mMSCs in vitro under the AKI microenvironment, which is mediated by EPOR and related with the proliferation/apoptosis signal pathway.


PubMed | 455th Hospital of PLA
Type: Journal Article | Journal: Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation | Year: 2012

The codes of medical consumptive materials is the base for standardized and information management. This article analyzes in detail the structure and meanings of five common-used classification codes in management including registration certificate code, bar code of high-value medical consumptive materials, charging code, medical insurance code and account code. The disadvantages of current coding method and solving program are suggested.


PubMed | 455th hospital of PLA
Type: Journal Article | Journal: Experimental biology and medicine (Maywood, N.J.) | Year: 2013

Bone-marrow mesenchymal stem cells (BMSCs) transplantation is effective for acute kidney injury (AKI) repair but with limited efficiency. In the present study, BMSCs marked by bromodeoxyuridine (BrdU) were transplanted to the AKI mouse model with erythropoietin (EPO) being subcutaneously injected. The blood urea nitrogen (BUN) and serum creatinine (Scr) levels, pathological changes, distribution of BMSCs, expressions of the cytokeratin 18 (CK18) and the stromal cell-derived factor 1 (SDF-1) in the nephridial tissues were measured. The directional migration of BMSCs to the AKI microenvironment invitro was also tested. The results showed that BMSCs transplantation or EPO injection alone decreased the BUN and Scr levels and the acute tubular necrosis (ATN) scoring in varied degrees. The combination of these decreased the above indicators levels significantly. BrdU(+) cells (BMSCs) were observed in the AKI nephridial tissues, and CK18 expressed in the cytoplasm of these cells. EPO injection increased the proportion of BrdU(+) cells with the enhanced expression of SDF-1 in the AKI nephridial tissues. EPO increased the migrating number of BMSCs to the AKI microenvironment invitro, and additional anti-SDF-1 treatment with SDF-1 antibody neutralized this effect. Our results showed that EPO increased the number of the transplanted BMSCs in the injured nephridial tissues and enhanced the AKI repair effect of BMSCs transplantation. The enhanced kidney-homing efficiency for BMSCs mediated by the SDF-1/CXCR4 pathway is one of the possible mechanisms for EPO performance.

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