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Song T.,Shanghai University | Yan X.,455 Hospital of PLA | Ye T.,Shanghai University
Knee Surgery, Sports Traumatology, Arthroscopy | Year: 2016

Purpose: The aim of this study is to compare the clinical and radiographic results and the complication rate between early and delayed surgical treatment of acromioclavicular joint (ACJ) dislocation. Methods: Publications in the management of ACJ dislocation are identified from the PubMed database between January 1993 and December 2013 using “acromioclavicular joint” and “dislocation” as keywords. The eligibility criteria included are as follows: (1) ACJ dislocation; (2) intervention, early compared with delayed surgical treatment or the surgical treatment for acute compared with chronic ACJ dislocation; (3) human; and (4) English articles. Exclusion criteria consist of the following: (1) type I and type II ACJ dislocation, (2) no definition of the time of early and delayed surgery in studies, (3) no comparison between the clinical result of early and delayed surgery in studies, (4) laboratory studies, radiographic studies, biomechanical studies, (5) the cases including fractures or revisions in studies, and (6) systematic analyses. Results: Eight studies comparing early and delayed surgical treatment of ACJ dislocation are included in this systematic review. According to Constant scores and shoulder subjective value, early surgery has better functional outcomes than delayed surgery in the treatment of ACJ dislocation (P < 0.05). Partial-dislocation/re-dislocation is found at 26.0 % in early and 38.1 % in delayed surgical treatment (P < 0.05). The rate of CC ossification in early surgical treatment is found as the same as the delayed. The complication rates are found at 12.5 % in early surgical treatment and 17.7 % in the delayed, which is not significantly different. Conclusion: Early surgical treatment may have superiority to the delayed procedure in the management of ACJ dislocation with better functional outcomes and more satisfied reduction. However, high-quality evidence studies are required to provide stronger support for this opinion in the future. Level of evidence: IV. © 2014, Springer-Verlag Berlin Heidelberg.


PubMed | 455 Hospital of PLA and Shanghai University
Type: Journal Article | Journal: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA | Year: 2016

The aim of this study is to compare the clinical and radiographic results and the complication rate between early and delayed surgical treatment of acromioclavicular joint (ACJ) dislocation.Publications in the management of ACJ dislocation are identified from the PubMed database between January 1993 and December 2013 using acromioclavicular joint and dislocation as keywords. The eligibility criteria included are as follows: (1) ACJ dislocation; (2) intervention, early compared with delayed surgical treatment or the surgical treatment for acute compared with chronic ACJ dislocation; (3) human; and (4) English articles. Exclusion criteria consist of the following: (1) type I and type II ACJ dislocation, (2) no definition of the time of early and delayed surgery in studies, (3) no comparison between the clinical result of early and delayed surgery in studies, (4) laboratory studies, radiographic studies, biomechanical studies, (5) the cases including fractures or revisions in studies, and (6) systematic analyses.Eight studies comparing early and delayed surgical treatment of ACJ dislocation are included in this systematic review. According to Constant scores and shoulder subjective value, early surgery has better functional outcomes than delayed surgery in the treatment of ACJ dislocation (P<0.05). Partial-dislocation/re-dislocation is found at 26.0% in early and 38.1% in delayed surgical treatment (P<0.05). The rate of CC ossification in early surgical treatment is found as the same as the delayed. The complication rates are found at 12.5% in early surgical treatment and 17.7% in the delayed, which is not significantly different.Early surgical treatment may have superiority to the delayed procedure in the management of ACJ dislocation with better functional outcomes and more satisfied reduction. However, high-quality evidence studies are required to provide stronger support for this opinion in the future.IV.


Li M.,455 Hospital of PLA | Liu X.,455 Hospital of PLA | Yue H.,455 Hospital of PLA | Xiong W.,455 Hospital of PLA | And 2 more authors.
Cellular Physiology and Biochemistry | Year: 2013

Background/Aims: Neural stem cells (NSCs) hold considerable potential as a therapeutic tool for repair of the damaged nervous system. In the current study, we examined whether transplanted N-acetyl aspartyl-glutamate synthetase (NAAGS)-activated NSCs (NAAGS/NSCs) further improve neurological recovery following traumatic brain injury (TBI) in Sprague-Dawley rats. Methods: Animals received TBI and stereotactic injection of NSCs, NAAGS/NSCs or phosphate buffered saline without cells (control) into the injured cortex. NAAGS protein expression was detected through western blot analysis. Dialysate NAAG levels were analyzed with radioimmunoassay. Cell apoptosis was detected via TUNEL staining. The expression levels of specific pro-inflammatory cytokines were detected with enzyme-linked immunosorbent assay. Results: Groups with transplanted NSCs and NAAGS/NSCs displayed significant recovery of the motor behavior, compared to the control group. At 14 and 21 days post-transplantation, the motor behavior in NAAGS/NSC group was significantly improved than that in NSC group (p<0.05). Additionally, transplanted NAAGS/NSCs inhibited cell apoptosis and the expression levels of specific pro-inflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor-α. Conclusion: Our results collectively demonstrate that NAAGS/NSCs provide a more powerful autoplastic therapy for the injured nervous system. © 2014 S. Karger AG, Basel.


Wang H.-L.,455 Hospital of PLA | Li Y.,455 Hospital of PLA | Tian J.,455 Hospital of PLA | Hu W.-F.,455 Hospital of PLA | Zhang J.-Y.,455 Hospital of PLA
Medical Journal of Chinese People's Liberation Army | Year: 2014

Objective To investigate the effects of high strength exhaustive exercise on apoptosis of renal tubular epithelial cells (TECs) and expression of hypoxia-inducible factor-1 α (HIF-1 α) in rats and the effect of intervention of total ginsenoside. Methods Thirty-five male Sprague-Dawley rats were randomly divided into five groups (7 each): control group (CTL), model group (divided into two subgroups: M2 and M24); total ginsenoside group (divided into two subgroups: G2 and G24). The animal model of exhaustive exercise induced acute kidney injury (AKI) was reproduced by forcing the rats running on the treadmill followed by exhaustive swimming according to the protocol. Ginsenoside [100g/(kg.d)] was intragastrically gavaged 5 days before exhausting exercise in rats of G2 and G24, and the same volume of water was given to rats in model and CTL groups. Apoptosis of TECs was observed under microscope after TUNEL staining. The expressions of caspase-3 and HIF-1a in TECs were analyzed by immunohistochemical staining. The protein expression of caspase-3 and HIF-1a was determined by Western blotting. Results In model group, 11 out of 14 rats were stranded on treadmill, and the time of exhaustive swimming was significantly shorter as compared with that of CTL group; but the endurance increased significantly in total ginsenoside groups, in which only 6/14 rats were stranded on treadmill, and the swimming time was significantly longer compared with model group (P<0.05). The TUNEL-positive apoptotic cells increased in M2 and M24 groups (especially in M24 group), and they were mainly distributed in those TECs at the corticomedullary junction in model groups; but the number of TUNEL-positive cells decreased significantly in total ginsenoside group (G2, G24) as compared with model group (P<0.05). Immunohistochemical analysis showed that caspases-3 and HIF-1α were only weakly positive in CTL group, but significantly upregulated in M2 group, and it was stronger in M24 group. Strongly positive staining of caspase 3 was observed in the brush border of injured TECs, and it decreased significantly in total ginsenoside group (G2 and G24). On the other hand, the level of HIF-1α expression increased in these groups compared with the model group. Western blotting showed trace expression of caspases-3 and HIF-1α in CTL group, but the level of caspases-3 expression significantly increased in model group (2.7-fold increase in M2 group, 3-fold increase in M24 group), while the caspases-3 expression levels were reduced about 58% and 61%, respectively, in G2 and G24 groups compared with M2 or M24 group. The HIF-1α expression increased to some extent in model group (M2 or M24), but HIF-1α expression increased rapidly in early stage, and it was in a high level in G24 in total ginsenoside group. Conclusions Exhaustive exercise may induce renal TECs apoptosis and increase caspases-3 expression; total ginsenosides can protect the TECs against injury by down-regulating caspases-3-dependent apoptotic signaling and rapid increase in HIF-lα expression. This might be one of the mechanisms of protection of tubular damage in acute kidney injury induced by exhaustive exercise by total ginsenosides.


Objective To determine the effects of Y chromosome microdeletion on the embryo development and clinical outcome of the ICSI treatment cycle. Methods The embryo development and clinical outcome of 19 patients with Y microdeletions who attempted 23 ICSI cycles at our center were retrospectively analyzed in the study. The patients in study group were matched to 86 cycles at the same time who had either severe oligozoospermia or azoospermia with normal Y chromosomes (control group). The differences between the two groups were compared including the average age, duration of infertility, the rate of natural abortion history, number of oocytes retrieved, fertilization rates、good embryo rate, number of embryos transferred, implantation rate, clinical pregnancy rate,early abortion rate. Results There were no significant differences between Groups in the rates of fertilization (85.0% vs 89.2%), the cleavage (96.0% vs 95.3%), good embryos (68.3% vs 66.7%), biochemical pregnancy (47.8% vs 50.0%), clinical pregnancy (43.5% vs 41.6%), implantation (22.9% vs 20.8%), and early abortion. (10.0% vs 7.5%) (P >0.05). Conclusion Y chromosome microdeletion has no significant effect on the embryo development and clinical outcome of the ICSI treatment cycle. © 2014, Shanghai Jiaotong University School of Medicine. All Rights Reserved.


PubMed | 455 Hospital of PLA
Type: Journal Article | Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | Year: 2014

Neural stem cells (NSCs) hold considerable potential as a therapeutic tool for repair of the damaged nervous system. In the current study, we examined whether transplanted N-acetyl aspartyl-glutamate synthetase (NAAGS)-activated NSCs (NAAGS/NSCs) further improve neurological recovery following traumatic brain injury (TBI) in Sprague-Dawley rats.Animals received TBI and stereotactic injection of NSCs, NAAGS/NSCs or phosphate buffered saline without cells (control) into the injured cortex. NAAGS protein expression was detected through western blot analysis. Dialysate NAAG levels were analyzed with radioimmunoassay. Cell apoptosis was detected via TUNEL staining. The expression levels of specific pro-inflammatory cytokines were detected with enzyme-linked immunosorbent assay.Groups with transplanted NSCs and NAAGS/NSCs displayed significant recovery of the motor behavior, compared to the control group. At 14 and 21 days post-transplantation, the motor behavior in NAAGS/NSC group was significantly improved than that in NSC group (p<0.05). Additionally, transplanted NAAGS/NSCs inhibited cell apoptosis and the expression levels of specific pro-inflammatory cytokines, including interleukin-1, interleukin-6 and tumor necrosis factor-.Our results collectively demonstrate that NAAGS/NSCs provide a more powerful autoplastic therapy for the injured nervous system.

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