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Nanjing, China

Qian J.,Changzheng Hospital | Luo Y.,454th Hospital of PLA | Gu X.,Changzheng Hospital | Wang X.,Changzheng Hospital
Cancer Biotherapy and Radiopharmaceuticals | Year: 2013

Hepatocellular carcinoma is the most common type of liver cancer. Radiotherapy combined with chemotherapy is the treatment of choice for hepatocellular carcinoma, but radioresistance of the cancer remains a significant therapeutic hindrance. Here, we provided several lines of evidence that small ubiquitin-like modifier (SUMO)-specific protease 6 (SENP6) could be an attractive molecular target for the treatment of hepatocellular carcinoma. By using immunohistochemical and real-time PCR, we showed that SENP6 was overexpressed in more than half of the hepatocellular carcinoma tissues. The growth retardation and radiosensitization were caused by silencing of SENP6 in the hepatocellular carcinoma cell lines using lentiviral shRNA. Moreover, SENP6 was required for radiation-induced NF-κB activation and the half-life of IκBα, a well-known inhibitor of NF-κB, and was extended by SENP6 silencing. Thus, our data demonstrated that SENP6 is an attractive drug target for anticancer therapy and radiosensitization. © Mary Ann Liebert, Inc.

Qiu Y.,454th Hospital of PLA | Zhu Y.-Y.,454th Hospital of PLA | Yan Y.-J.,Donghua University | Chen N.,Donghua University | Chen Z.-L.,Donghua University
Blood Coagulation and Fibrinolysis | Year: 2014

Haemorrhage is the major cause of death in civilian trauma and the leading cause of preventable death in military trauma. It is very important to develop a haemostatic material with definite haemostatic effects. In this study, a nano-fabric membrane containing fibrinogen (Fbg) (2.5%, w/v) was successfully prepared by electrospinning as a haemostatic dressing. The average fibre diameter was 400nm by scanning electron microscope (SEM), and it was indicated that fibrinogen and fibrin possessed excellent compatibility with poly (L-lactic)-acid (PLLA) from X-ray diffraction (XRD). Swine traumatic haemorrhage models including spleen haemorrhage, liver haemorrhage and femoral arteriovenous fistula haemorrhage were developed to detect haemostatic effects of this dressing. The results showed that the Fbg-loaded PLLA nano-fibre can significantly decrease the bleeding time, blood loss and mortality rate, which suggested that Fbg-loaded PLLA nano-fibre was efficacious on the models of traumatic uncontrolled haemorrhage, and further study of this dressing would be warranted to determine its potential in first aid and field trauma care. Copyright © 2014 Lippincott Williams & Wilkins.

Huo Z.,454th Hospital of PLA | Qiu Y.,454th Hospital of PLA | Chu Z.,454th Hospital of PLA | Yin P.,454th Hospital of PLA | And 4 more authors.
Journal of Nanomaterials | Year: 2015

Poly(L-lactic)-acid (PLLA) as a drug carrier and a water-soluble drug timosaponin B-II (TB-II) as a model drug were selected to prepare drug-loaded nanofibers by electrospinning. The average diameters of pure PLLA nanofibers and TB-II-loaded nanofibers were 212.5 ± 68.5, 219.7 ± 57.8, 232.8 ± 66.9, and 232.9 ± 97.7 nm, respectively. DSC and XRD results demonstrated that TB-II was well incorporated into the nanofibers in an amorphous state. FI-TR spectroscopy indicated that TB-II had good compatibility with PLLA. In vitro release studies showed that TB-II was rapidly released from the nanofibers within 6 h, followed by a gradual release for long time. In vivo biosafety test revealed no noticeable toxicity of these TB-II nanofibers. The TB-II released from the nanofibers had obvious inhibition effect against human hepatocellular carcinoma SMMC 7721 cells both in vivo and in vitro. It was confirmed that the TB-II-loaded nanofibers were a sustained delivery system which could effectively inhibit the tumor growth and recurrence after surgery. © 2015 Zhonghua Huo et al.

Huo Z.-H.,454th Hospital of PLA | Chu Z.-L.,454th Hospital of PLA | Hu J.,454th Hospital of PLA | Song B.,454th Hospital of PLA | Lv S.,454th Hospital of PLA
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2012

Objective: To investigate the effects of miRNA overexpression on drug resistance of cis-diamminedichloroplatinum (CDDP) resistant gastric cancer cell line BGC-823/CDDP. Methods: Human genomic DNA was extracted, PCR primers targeting human miRNA765 were designed, and gene sequence containing miRNA765 precursor was amplified by PCR and cloned into an eukaryotic expression vector to construct a recombinant miRNA expression vector. BGC-823/CDDP cells were transfected with the recombinant plasmid using cationic liposome. Forty-eight hours after transfection, the relative contents of miRNA765 and cytokine-induced apoptosis inhibitor 1 (CIAPIN1) protein in cells were determined by Real-Time PCR and Western blotting. Forty-eight hours after transfection, the cells were treated with different concentrations of CDDP. Twenty-four and forty-eight hours later, cell viability was detected using MTT assay, and 50% concentration of inhibition (IC50) of the cells to CDDP was calculated. BGC-823/CDDP cells of gene intervention were treated with CDDP at a final concentration of 1 μg/mL, and double staining method was used to detect the cell apoptosis rate. Results: The relative expression of miRNA765 in BGC-823/CDDP cells was significantly lower than that in parental cell line BGC-823 (P<0.01), and the relative expression of CIAPIN1 protein in BGC-823/CDDP cells was significantly higher than that of parental cell line (P<0.01). The overexpression of miRNA765 significantly inhibited the expression of CIAPIN1 protein in BGC-823/CDDP cells. Forty-eight hours after transfection, the expression of CIAPIN1 protein was significantly lower than that of the untransfected group (P<0.01). The overexpression of miRNA765 significantly reduced the drug resistance of BGC-823/CDDP cells, and 48-hour IC50 value was reduced from (18.27±3.92) μg/mL to (1.50±0.43) μg/mL (P<0.05). Forty-eight hours after transfection, the apoptosis rate of BGC-823/CDDP cells was significantly increased from (10.1±1.7)% to (53.4±7.9)%(P<0.01). Conclusion: The overexpression of miRNA765 may significantly reduce the drug resistance of resistant gastric carcinoma BGC-823/CDDP cells.

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