454th Hospital of PLA

Nanjing, China

454th Hospital of PLA

Nanjing, China

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Huo Z.,454th Hospital of PLA | Qiu Y.,454th Hospital of PLA | Chu Z.,454th Hospital of PLA | Yin P.,454th Hospital of PLA | And 4 more authors.
Journal of Nanomaterials | Year: 2015

Poly(L-lactic)-acid (PLLA) as a drug carrier and a water-soluble drug timosaponin B-II (TB-II) as a model drug were selected to prepare drug-loaded nanofibers by electrospinning. The average diameters of pure PLLA nanofibers and TB-II-loaded nanofibers were 212.5 ± 68.5, 219.7 ± 57.8, 232.8 ± 66.9, and 232.9 ± 97.7 nm, respectively. DSC and XRD results demonstrated that TB-II was well incorporated into the nanofibers in an amorphous state. FI-TR spectroscopy indicated that TB-II had good compatibility with PLLA. In vitro release studies showed that TB-II was rapidly released from the nanofibers within 6 h, followed by a gradual release for long time. In vivo biosafety test revealed no noticeable toxicity of these TB-II nanofibers. The TB-II released from the nanofibers had obvious inhibition effect against human hepatocellular carcinoma SMMC 7721 cells both in vivo and in vitro. It was confirmed that the TB-II-loaded nanofibers were a sustained delivery system which could effectively inhibit the tumor growth and recurrence after surgery. © 2015 Zhonghua Huo et al.

Qian J.,Changzheng Hospital | Luo Y.,454th Hospital of PLA | Gu X.,Changzheng Hospital | Wang X.,Changzheng Hospital
Cancer Biotherapy and Radiopharmaceuticals | Year: 2013

Hepatocellular carcinoma is the most common type of liver cancer. Radiotherapy combined with chemotherapy is the treatment of choice for hepatocellular carcinoma, but radioresistance of the cancer remains a significant therapeutic hindrance. Here, we provided several lines of evidence that small ubiquitin-like modifier (SUMO)-specific protease 6 (SENP6) could be an attractive molecular target for the treatment of hepatocellular carcinoma. By using immunohistochemical and real-time PCR, we showed that SENP6 was overexpressed in more than half of the hepatocellular carcinoma tissues. The growth retardation and radiosensitization were caused by silencing of SENP6 in the hepatocellular carcinoma cell lines using lentiviral shRNA. Moreover, SENP6 was required for radiation-induced NF-κB activation and the half-life of IκBα, a well-known inhibitor of NF-κB, and was extended by SENP6 silencing. Thus, our data demonstrated that SENP6 is an attractive drug target for anticancer therapy and radiosensitization. © Mary Ann Liebert, Inc.

Xu X.-H.,454th Hospital of PLA | Xu K.,Wuhan General Hospital of Guangzhou Military | Peng Q.,454th Hospital of PLA | Xue Y.-C.,454th Hospital of PLA | Pan X.-F.,454th Hospital of PLA
International Eye Science | Year: 2016

AIM: To compare the effectiveness of botulinus toxin of type A and complete resection of the periorbital muscle on idiopathic blepharospasm. METHODS: Patients with idiopathic blepharospasm and having undergone either of two procedures from Dec. 2010 to Jun. 2015 were selected (60 patients). Among them, group A (30 patients, 60 eyes) underwent botulinus toxin of type A, group B (30 patients, 60 eyes) underwent complete resection of the periorbital muscle. RESULTS: In group A, the patients with complete response, obvious response, partial response, and no response were 36(60.0%), 20(33.3%), 2(3.3%) and 2(3.3%) cases respectively. In group B, the patients with complete response, obvious response, partial response, and no response were 16(26.7%), 24(40.0), 12(20.0%) and 8(13.3%) cases respectively. The difference was statistically significant (Z=-2.968, P=0.003). The relapse rate of group A and group B were 93.3% and 20.0% after 6mo, the difference was statistically significant (χ2=32.851, P<0.001). CONCLUSION: The botulinus toxin injection of type A is effective for idiopathic blepharospasm. But recurrence rate is high after 6mo. Complete resection of the periorbital muscle have long-term efficacy for idiopathic blepharospasm. It's a supplementary therapy after idiopathic blepharospasm recurrence. Copyright 2016 by the IJO Press.

Huo Z.-H.,454th Hospital of PLA | Chu Z.-L.,454th Hospital of PLA | Hu J.,454th Hospital of PLA | Song B.,454th Hospital of PLA | Lv S.,454th Hospital of PLA
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2012

Objective: To investigate the effects of miRNA overexpression on drug resistance of cis-diamminedichloroplatinum (CDDP) resistant gastric cancer cell line BGC-823/CDDP. Methods: Human genomic DNA was extracted, PCR primers targeting human miRNA765 were designed, and gene sequence containing miRNA765 precursor was amplified by PCR and cloned into an eukaryotic expression vector to construct a recombinant miRNA expression vector. BGC-823/CDDP cells were transfected with the recombinant plasmid using cationic liposome. Forty-eight hours after transfection, the relative contents of miRNA765 and cytokine-induced apoptosis inhibitor 1 (CIAPIN1) protein in cells were determined by Real-Time PCR and Western blotting. Forty-eight hours after transfection, the cells were treated with different concentrations of CDDP. Twenty-four and forty-eight hours later, cell viability was detected using MTT assay, and 50% concentration of inhibition (IC50) of the cells to CDDP was calculated. BGC-823/CDDP cells of gene intervention were treated with CDDP at a final concentration of 1 μg/mL, and double staining method was used to detect the cell apoptosis rate. Results: The relative expression of miRNA765 in BGC-823/CDDP cells was significantly lower than that in parental cell line BGC-823 (P<0.01), and the relative expression of CIAPIN1 protein in BGC-823/CDDP cells was significantly higher than that of parental cell line (P<0.01). The overexpression of miRNA765 significantly inhibited the expression of CIAPIN1 protein in BGC-823/CDDP cells. Forty-eight hours after transfection, the expression of CIAPIN1 protein was significantly lower than that of the untransfected group (P<0.01). The overexpression of miRNA765 significantly reduced the drug resistance of BGC-823/CDDP cells, and 48-hour IC50 value was reduced from (18.27±3.92) μg/mL to (1.50±0.43) μg/mL (P<0.05). Forty-eight hours after transfection, the apoptosis rate of BGC-823/CDDP cells was significantly increased from (10.1±1.7)% to (53.4±7.9)%(P<0.01). Conclusion: The overexpression of miRNA765 may significantly reduce the drug resistance of resistant gastric carcinoma BGC-823/CDDP cells.

Qiu Y.,454th Hospital of PLA | Zhu Y.-Y.,454th Hospital of PLA | Yan Y.-J.,Donghua University | Chen N.,Donghua University | Chen Z.-L.,Donghua University
Blood Coagulation and Fibrinolysis | Year: 2014

Haemorrhage is the major cause of death in civilian trauma and the leading cause of preventable death in military trauma. It is very important to develop a haemostatic material with definite haemostatic effects. In this study, a nano-fabric membrane containing fibrinogen (Fbg) (2.5%, w/v) was successfully prepared by electrospinning as a haemostatic dressing. The average fibre diameter was 400nm by scanning electron microscope (SEM), and it was indicated that fibrinogen and fibrin possessed excellent compatibility with poly (L-lactic)-acid (PLLA) from X-ray diffraction (XRD). Swine traumatic haemorrhage models including spleen haemorrhage, liver haemorrhage and femoral arteriovenous fistula haemorrhage were developed to detect haemostatic effects of this dressing. The results showed that the Fbg-loaded PLLA nano-fibre can significantly decrease the bleeding time, blood loss and mortality rate, which suggested that Fbg-loaded PLLA nano-fibre was efficacious on the models of traumatic uncontrolled haemorrhage, and further study of this dressing would be warranted to determine its potential in first aid and field trauma care. Copyright © 2014 Lippincott Williams & Wilkins.

PubMed | 454th Hospital of PLA and General Hospital of Lanzhou
Type: | Journal: Physiology & behavior | Year: 2016

Exposure to hypobaric hypoxia causes oxidative stress, neuronal degeneration and apoptosis that leads to memory impairment. Though oxidative stress contributes to neuronal degeneration and apoptosis in hypobaric hypoxia, the ability for phenylethanoid glycosides of Pedicularis muscicola Maxim (PhGs) to reverse high altitude memory impairment has not been studied. Rats were supplemented with PhGs orally for a week. After the fourth day of drug administration, rats were exposed to a 7500 m altitude simulation in a specially designed animal decompression chamber for 3 days. Spatial memory was assessed by the 8-arm radial maze test before and after exposure to hypobaric hypoxia. Histological assessment of neuronal degeneration was performed by hematoxylin-eosin (HE) staining. Changes in oxidative stress markers and changes in the expression of the apoptotic marker, caspase-3, were assessed in the hippocampus. Our results demonstrated that after exposure to hypobaric hypoxia, PhGs ameliorated high altitude memory impairment, as shown by the decreased values obtained for reference memory error (RME), working memory error (WME), and total error (TE). Meanwhile, administration of PhGs decreased hippocampal reactive oxygen species levels and consequent lipid peroxidation by elevating reduced glutathione levels and enhancing the free radical scavenging enzyme system. There was also a decrease in the number of pyknotic neurons and a reduction in caspase-3 expression in the hippocampus. These findings suggest that PhGs may be used therapeutically to ameliorate high altitude memory impairment.

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