451 Hospital of Chinese PLA

Fengcheng, China

451 Hospital of Chinese PLA

Fengcheng, China
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Bai J.-X.,PLA Fourth Military Medical University | Bai J.-X.,General Hospital of Shenyang Military Command | Yan B.,PLA Fourth Military Medical University | Zhao Z.-N.,PLA Fourth Military Medical University | And 10 more authors.
Endocrinology | Year: 2013

Although tamoxifen (TAM), a selective estrogen receptor modulator, has been widely used in the treatment of hormone-responsive breast cancer, its estrogen-like effect increases the risk of endometrial cancer. However, the molecular mechanisms of TAM-induced endometrial carcinoma still remain unclear. In this report, we explored the role of microRNAs (miRNAs) in TAM-induced epithelial-mesenchymal transition (EMT) in ECC-1 and Ishikawa endometrial cancer cell lines and found miR-200 is involved in this process via the regulation of c-Myc. When treated with TAM, ECC-1 and Ishikawa cells were characterized by higher invasiveness and motility and underwent EMT. miR-200, a miRNA family with tumor suppressive functions in a wide range of cancers, was found reduced in response to TAM treatment. Consistent with zinc finger E-box binding homeobox 2, which was confirmed as a direct target of miR-200b in endometrial cancer cell lines, some other key factors of EMT such as Snail and N-cadherin increased, whereas E-cadherin decreased in the TAM-treated cells, contributing to TAM-induced EMT in these endometrial cancer cells. In addition, we showed that c-Myc directly binds to and represses the promoter of miR-200 miRNAs, and its up-regulation in TAM-treated endometrial cancer cells leads to the down-regulation of miR-200 and eventually to EMT. Collectively, our data suggest that TAM can repress the miR-200 family and induce EMT via the up-regulation of c-Myc in endometrial cancer cells. These findings describe a possible mechanism of TAM-induced EMT in endometrial cancer and provide a potential new therapeutic strategy for it. Copyright © 2013 by The Endocrine Society.

Zhao Z.-N.,PLA Fourth Military Medical University | Zhao Z.-N.,451 Hospital of Chinese PLA | Zhou Q.,PLA Fourth Military Medical University | Bai J.-X.,PLA Fourth Military Medical University | And 6 more authors.
Cancer Biology and Therapy | Year: 2011

The cascade of caspase processing and activation is the core of apoptotic cell signaling. Initiator caspases are activated by adaptor-mediated clustering, which allows the intermolecular autoprocessing of the zymogens in close proximity. Caspase-8, the prototypical initiator critically involved in apoptosis induced by varied extrinsic stimuli, is physiologically recruited via a classical FasL/Fas/FADD pathway. Meanwhile, artificial models of caspase-8 activation have been proposed via inducible dimerization of a heterologous domain fused to the zymogen. Here, we describe the generation of a chimeric protein of caspase-8 and the ligand-binding domain (LBD) of estrogen receptor α (ERα), which dimerizes and undergoes autocleavage upon engagement by the ligand, estradiol. Breast cancer cells expressing this fusion protein exhibit apoptotic cell death in vitro and suppressed tumor growth in xenograft nude mice models in response to the administration of estrogen. Thus, the suicidal caspase-8/ERα-LBD protein, which reverses the mitogenic effects of estrogens, has potential to provide novel approaches to treating estrogen-dependent and -independent breast cancers. © 2011 Landes Bioscience.

Zhou Q.,PLA Fourth Military Medical University | Zhao Z.-N.,451 Hospital of Chinese PLA | Cheng J.-T.,PLA Fourth Military Medical University | Zhang B.,PLA Fourth Military Medical University | And 4 more authors.
Biochemical and Biophysical Research Communications | Year: 2011

Bisphosphonates (BPs) have a profound effect on bone resorption and are widely used to treat osteoclast-mediated bone diseases. They suppress bone resorption by inhibiting the activity of mature osteoclasts and/or the formation of new osteoclasts. Osteoblasts may be an alternative target for BPs. Periodontal ligament stem cells (PDLSCs) exhibit osteoblast-like features and are capable of differentiating into osteoblasts or cementoblasts. This study aimed to determine the effects of ibandronate, a nitrogen-containing BP, on the proliferation and the differentiation of PDLSCs and to identify the microRNAs (miRNAs) that mediate these effects. The PDLSCs were treated with ibandronate, and cell proliferation was measured using the MTT (3-dimethylthiazol-2,5-diphenyltetrazolium bromide) assay. The expression of genes and miRNAs involved in osteoblastic differentiation was assayed using quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). Ibandronate promoted the proliferation of PDLSCs and enhanced the expression of alkaline phosphatase (ALP), type I collagen (COL-1), osteoprotegerin (OPG), osteocalcin (OCN), and Runx2. The expression of miRNAs, including miR-18a, miR-133a, miR-141 and miR-19a, was significantly altered in the PDLSCs cultured with ibandronate. In PDLSCs, ibandronate regulates the expression of diverse bone formation-related genes via miRNAs. The exact mechanism underlying the role of ibandronate in osteoblasts has not been completely understood. Ibandronate may suppress the activity of osteoclasts while promoting the proliferation of osteoblasts by regulating the expression of miRNAs. © 2010 Elsevier Inc.

Chen R.-B.,451 Hospital of Chinese PLA | Fan S.-D.,451 Hospital of Chinese PLA | Hu W.-H.,451 Hospital of Chinese PLA | Wang R.,451 Hospital of Chinese PLA | And 3 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: In recent years artificial joint replacement surgery has become a new method for the treatment of intertrochanteric fractures of the elderly. In the traditional treatment of internal fixation of senile intertrochanteric fractures, the prosthesis is implanted following fracture treatment, or the small trochanter is treated and the prosthesis is implanted, followed by greater trochanter treatment. It remains controversial about the application of lengthened or standard length stem. OBJECTIVE: To evaluate the effect of artificial joint replacement on elderly patients with unstable intertrochanteric fracture, and observe the influence of the order of prosthesis implantation, small trochanter and greater trochanter treatment during the surgery. METHODS: The clinical data of 28 elderly patients with unstable femoral intertrochanteric fractures treated in Department of Orthopedics, the 451 Hospital of Chinese PLA from January 2006 to December 2008 were retrospectively analyzed, including 20 undergoing artificial joint replacement and 8 undergoing total hip replacement with cemented prosthesis. X-ray, Harris scores of hip joint and complications were observed postoperatively. RESULTS AND CONCLUSION: All patients were followed up for 1 to 4 years (average 2.8 years), with an excellent and good rate of 89.3%. No coxa vara, infection, loosening or dislocation was found. The surgery can quickly restore limb function and reduce complications. The treatment of greater and small tuberosity fracture and prosthesis is very important. The prosthesis is firstly implanted, followed by internal fixation with the prosthesis as the support. Tightly matching of the proximal femur and prosthesis is essential for the stability of postoperative prosthesis, and lengthened or standard length stem can be used.

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