Shanghai, China
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Song C.-Y.,Shanghai University | Zeng X.,Shanghai University | Chen S.-W.,Nanlou General Hospital of PLA | Hu P.-F.,Shanghai University | And 5 more authors.
Journal of Gastroenterology and Hepatology (Australia) | Year: 2011

Background and Aim: Non-alcoholic steatohepatitis (NASH) is one entity in the spectrum of non-alcoholic fatty liver disease (NAFLD). The aim of this study was to explore the prevention and therapeutic effect of sophocarpine on experimental rat NASH. Methods: Sophocarpine with the dosage of 20mg/kg/day was injected into NASH rats. At the end of 12weeks, all rats were killed to detect the degree of fatty degeneration, inflammation and fibrosis. Results: Sophocarpine intervention (in the pro-treated and treated groups) resulted in a significant decrease of liver weight, liver index, serum transaminase and serum lipids. Messenger RNA expressions of leptin, interleukin (IL)-6, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1, procollagen-I and α-smooth muscle actin (SMA) and deposition of IL-6, TNF-α and TGF-β1 in liver decreased, whereas the messenger RNA expression of adiponectin increased significantly compared with that in the model group. Moreover, histological improvement was also observed in the sophocarpine intervention group. In addition, there was no significant difference in any detected indicator between the pro-treated and treated group. Conclusions: Sophocarpine could decrease the level of serum transaminase, improve lipid metabolism, reduce synthesis of inflammatory cytokines TNF-α, TGF-β1 and IL-6, activate protective adipocytokine adiponectin, and might be selected as a promising agent for the clinical prevention and therapy of NASH. © 2011 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.


Huang G.,Shanghai University | Huang G.,411th Hospital of PLA | Liu X.,Shanghai University | Jiao L.,Shanghai University | And 7 more authors.
European Journal of Pharmacology | Year: 2014

Timely evaluation of the response to endocrine therapy in patients with prostate cancer (PCa) may optimize treatment regimens and improve long-term prognosis. We used the liquid chromatography-mass spectrometry (LC-MS)-based metabolomic technique to identify serum biomarkers indicative of disease progression and therapeutic benefit. The mean serum levels of seven metabolites, including deoxycholic acid (DCA), glycochenodeoxycholate (GCDC), l-tryptophan, docosapentaenoic acid (DPA), arachidonic acid, deoxycytidine triphosphate, and pyridinoline, differed significantly between untreated PCa patients and healthy controls. In patients who did not develop castration-resistant prostate cancer (CRPC) for at least 2 years (good responders), these metabolite levels reverted to near healthy control levels during endocrine therapy. In contrast, the metabolite levels remained abnormal in patients who developed CRPC within 1 year (poorly responsive patients). Three of these biomarkers (DCA, GCDC, and DPA) are mainly involved in cholesterol metabolism, underscoring the importance of elevated cholesterol to PCa progression. These metabolites may serve as predictive biomarkers for assessing the therapeutic response of PCa patients to endocrine therapy. © 2014 Elsevier B.V.


Qian H.,Shanghai University | Qian H.,411th Hospital of PLA | Deng X.,Shanghai University | Huang Z.-W.,Shanghai University | And 13 more authors.
Cell Research | Year: 2015

Hepatocytes are critical for the maintenance of liver homeostasis, but its involvement in hepatic fibrogenesis remains elusive. Hepatocyte nuclear factor 1α (HNF1α) is a liver-enriched transcription factor that plays a key role in hepatocyte function. Our previous study revealed a significant inhibitory effect of HNF1α on hepatocellular carcinoma. In this study, we report that the expression of HNF1α is significantly repressed in both human and rat fibrotic liver. Knockdown of HNF1α in the liver significantly aggravates hepatic fibrogenesis in either dimethylnitrosamine (DMN) or bile duct ligation (BDL) model in rats. In contrast, forced expression of HNF1α markedly alleviates hepatic fibrosis. HNF1α regulates the transcriptional expression of SH2 domain-containing phosphatase-1 (SHP-1) via directly binding to SHP-1 promoter in hepatocytes. Inhibition of SHP-1 expression abrogates the anti-fibrotic effect of HNF1α in DMN-treated rats. Moreover, HNF1α repression in primary hepatocytes leads to the activation of NF-κB and JAK/STAT pathways and initiates an inflammatory feedback circuit consisting of HNF1α, SHP-1, STAT3, p65, miR-21 and miR-146a, which sustains the deregulation of HNF1α in hepatocytes. More interestingly, a coordinated crosstalk between hepatocytes and hepatic stellate cells (HSCs) participates in this positive feedback circuit and facilitates the progression of hepatocellular damage. Our findings demonstrate that impaired hepatocytes play an active role in hepatic fibrogenesis. Early intervention of HNF1α-regulated inflammatory feedback loop in hepatocytes may have beneficial effects in the treatment of chronic liver diseases. © 2015 IBCB, SIBS, CAS.


Li Y.,411th Hospital of PLA | Huang Y.,Shanghai University | Cao Y.-S.,411th Hospital of PLA | Zeng J.,411th Hospital of PLA | And 3 more authors.
Tumor Biology | Year: 2013

X-ray repair cross-complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the base excision repair and plays an important role in the maintenance of genomic integrity. Polymorphisms in XRCC1 may alter the function and repair capacity of XRCC1 protein which further results in the genetic instability and lung carcinogenesis. Previous studies investigating the relationship between XRCC1 Arg399Gln polymorphism and lung cancer risk in Chinese yielded contradictory results. A meta-analysis was performed to clarify the effect of XRCC1 Arg399Gln polymorphism on lung cancer. The association was assessed by calculating the pooled odds ratio (OR) with 95 % confidence intervals (95 %CI). Nineteen studies with a total of 12,835 participants were included into this meta-analysis. Overall, there was an obvious association between XRCC1 Arg399Gln polymorphism and increased risk of lung cancer under three genetic models (Gln vs. Arg: OR = 1.13, 95 %CI 1.01-1.25, P = 0.029; GlnGln vs. ArArg: OR = 1.41, 95 %CI 1.07-1.84, P = 0.013; GlnGln vs. ArArg/ArgGln: OR = 1.37, 95 %CI 1.07-1.76, P = 0.013). Meta-analysis of 18 studies with high quality also found that there was an obvious association between XRCC1 Arg399Gln polymorphism and increased risk of lung cancer under three genetic models. There was no obvious risk of bias in the meta-analysis. Data from the current meta-analysis support the obvious association between XRCC1 Arg399Gln polymorphism and increased risk of lung cancer in Chinese. © 2013 International Society of Oncology and BioMarkers (ISOBM).


Li A.,Tongji University | Ji C.,Tongji University | Wang H.,Tongji University | Lang G.,411th Hospital of PLA | And 5 more authors.
BMC Urology | Year: 2015

Background: The treatment of large volume bladder stones by current equipments continues to be a management problem in both developing and developed countries. AH-1 Stone Removal System (SRS) invented by us is primarily used to crush and retrieve bladder stones. This study evaluated the safety and efficiency of transurethral cystolitholapaxy with SRS for the treatment of bladder stones of variable size. Methods: SRS, which was invented by Aihua Li in 2007, composed by endoscope, continuous-flow component, a jaw for stone handling and retrieving, lithotripsy tube, handle, inner sheath and outer sheath. 112 patients with bladder stones were performed by transurethral cystolitholapaxy with SRS since 2008. We compare the surgical outcome to bladder stones of variable size, and evaluate the surgical efficiency and safety. Results: Characteristics of patients and stone removal time in variable size were evaluated. To patients with single stone, stone size was 1.35 ± 0.37 cm and the operating time was 5.50 ± 3.92 min in Group A. Stone size was 2.38 ± 0.32 cm and the operating time was 11.90 ± 9.91 min in Group B. Stone size was 3.30 ± 0.29 cm and the operating time was 21.92 ± 9.44 min in Group C. Stone size was 4.69 ± 0.86 cm and the operating time was 49.29 ± 30.47 min in Group D. The difference was statistically significant between the four groups. Among them, 74 (66.07%) patients accompanied with benign prostatic hyperplasia (BPH) were treated by transurethral resection of the prostate (TURP) simultaneously. Compared between the four groups, the difference of the TURP time was not statistically significant, P >0.05. No significant complication was found in the surgical procedure. Conclusions: Transurethral cystolitholapaxy with SRS appears to be increased rapidity of the procedure with decreased morbidity. It is a safe and efficient surgical management to bladder stones. This endoscopic surgery best fits the ethics principle of no injury; meanwhile, the accompanied BPH could be effectively treated by TURP simultaneously. © 2015 Li et al.; licensee BioMed Central.


Gang H.,411th Hospital of PLA | Genqiang L.,411th Hospital of PLA | Yifeng Z.,411th Hospital of PLA | Jian C.,411th Hospital of PLA | And 3 more authors.
Chinese Journal of Andrology | Year: 2014

Objective To investigate the reason and treatment of varicocele recurrence after laparoscopic varicocelectomy. Methods A total of 33 patients with varicocele recurrence after laparoscopic varicocelectomy underwent subinguinal microscopic varicocelectomy. Results All the operations were successful, and there was no recurrent varicocele during the postoperative follow-up(6 months). Three months later, the semen quality of 27 patients were enhanced(81.8%). Conclusion The significant roles of spermaductal and cremaster veins in varicocele recurrence after laparoscopic varicocelectomy were supported. Preoperative pressure test of internal inguinal ring could reduce postoperative recurrence by selecting the appropriate operation method. Subinguinal microscopic varicocelectomy was an effective method for the treatment of varicocele recurrence after laparoscopic varicocelectomy. ©, 2015. Shanghai Jiaotong University School of Medicine. All rights reserved.


Wang X.-D.,411th Hospital of PLA | Sheng M.-J.,411th Hospital of PLA | Lin A.-J.,411th Hospital of PLA
Chinese Ophthalmic Research | Year: 2010

Background: Researches showed that incidence of dry eye is an ascending tendency in China recently. Establishing an ideal animal model of dry eye is helpful for us to study the mechanism of dry eye. Objective: The aim of this study was to establish an animal model of dry eye induced by meibomian gland dysfunction. Methods: The animal model of dry eye induced by meibomian gland dysfunction was established by cauterizing the meibomian gland orifices and excising the tertiary eyelid in the left eyes of 10 healthy clean New Zealand white rabbits, and the fellow eyes were used as the control eyes. Schirmer I test and ocular surface fluorescence stain were performed before surgery and 1 day, 4,11,18,25,32 days after surgery, and conjunctiva impression cytology was curried out before surgery and 7,14,28,40 days after surgery. The score of ocular surface fluorescence stain based on the van Bijsterveld's criteria. Animal models were sacrificed in 40 days after surgery and biopsy of cornea and conjunctival tissue was carried out to evaluate the pathological change under the optical microscope. The use of the experimental animals followed the Standard of Association for Research in Vision and Ophthalmology. Results: All of the meibomian gland channels were closed in the first day after operation. No significant differences were found in Schirmer I test between model group and control group (F = 0.009, P = 0.927) and among the different time points (F = 0.667, P = 0.652). The score of ocular surface fluorescence staining was increased in 4 days after operation in comparison to before operation (t = 4.0, P < 0.05) and control group (t = 4.0, P < 0.05). The conjunctiva goblet cell density decreased significantly in model group in 14 days after operation compared with control group (t = 8.3, P < 0.05), and the significant declined values also were seen in model group with control group from 14 days through 40 days after operation (P < 0.05). The infiltration of lots of inflammatory cells was seen in the subconjunctival tissue, and the continuity of corneal epithelium was destroyed in model eye in 40 days after operation under the optical microscope. Conclusion: Cauterizing meibomian gland orifices and excising the tertiary eyelid can establish rabbit dry eye model successfully with good stability and reliability.

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