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Prosser, WA, United States

Swisher K.D.,4106 North Bunn Road | Crosslin J.M.,4106 North Bunn Road
Southwestern Entomologist | Year: 2014

A restriction digestion method has been developed for haplotyping the potato psyllid, Bactericera cockerelli Šulc., an economically important pest of solanaceous crops. This method differentiates the four known potato psyllid haplotypes by using restriction enzyme digestion of a portion of the mitochondrial large subunit ribosomal RNA, tRNA-Val, and a portion of the small subunit ribosomal RNA, providing a second target within the mitochondrial DNA for haplotyping studies. This technique also provides a good alternative to the current method of potato psyllid haplotyping which uses high-resolution melting analysis, a technique that requires access to a real-time PCR machine with high-resolution melting capabilities. Potato psyllid haplotyping by restriction digestion is done using basic laboratory equipment that is readily available for smaller laboratories with budgetary limitations, thereby providing an excellent tool for laboratories of all sizes to identify psyllid populations. Source

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