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Qingdao, China

Shen E.,First Peoples Hospital of Yueyang | Liu C.,Shanghai University | Wei L.,401 Hospital of PLA | Hu J.,First Peoples Hospital of Yueyang | And 3 more authors.
Tumor Biology

Background: Published data regarding the association between the APE1 Asp148Glu polymorphism and colorectal cancer susceptibility remained controversial. This meta-analysis of literatures was performed to draw a more precise estimation of the relationship. Materials and methods: We systematically searched PubMed, Embase, and Web of Science with a time limit of August 19, 2013. Summary odds ratios (ORs) with 95 % CIs were used to assess the strength of association between the APE1 Asp148Glu polymorphism and colorectal cancer susceptibility using random-effects model. Results: A total of eight case-control studies including 2,597 cases and 3,063 controls were included for analysis. Overall, no significant associations were found between the APE1 Asp148Glu polymorphism and colorectal cancer susceptibility for GG vs TT (OR = 1.00, 95 % CI = 0.73-1.36, p = 0.00 for heterogeneity), TG vs TT (OR = 1.17, 95 % CI = 0.88-1.55, p = 0.00 for heterogeneity), the dominant model GG + TG vs TT (OR = 1.21, 95 % CI = 0.91-1.60, p = 0.00 for heterogeneity) nor the recessive model GG vs TG + TT(OR = 0.95, 95 % CI = 0.75-1.20, p = 0.02 for heterogeneity). In subgroup analysis, no significant associations were found in the Asian or Caucasian populations. Conclusion: This meta-analysis suggested that the APE1 Asp148Glu polymorphism was not associated with colorectal cancer susceptibility among Asians or Caucasians. © 2013 International Society of Oncology and BioMarkers (ISOBM). Source

Zhang W.,401 Hospital of PLA | Yu W.-J.,Qingdao University | Li Y.-J.,Qingdao University
Chinese Journal of Pathology

Objective To study the clinicopathologic features, immunohistochemical profiles and prognosis of chromophobe renal cell carcinoma (ChRCC). Methods Forty-two cases of ChRCC were retrieved from the archival files of the Affiliated Hospital of Qingdao University, 401 Hospital of PLA and Qingdao Municipal Hospital from 2003 to 2011. The clinical and pathologic features of the tumors were reviewed. Hale colloidal iron staining was performed and EnVision immunohistochemistry was used to detect the expression of a series of immunologic markers. Forty cases of clear cell renal cell carcinoma and 10 cases of renal oncocytoma were selected as controls. Results The patients included 17 males and 25 females. The age of patients ranged from 39 years to 78 years ( median age = 57 years ). On gross examination, the tumors ranged from 2 cm to 19 cm in greatest dimension (mean size =7. 3 cm). Histologically, the tumors were mainly composed of solid sheets, acini or tubules of malignant cells. The tumor cells contained clear finely reticular ("chromophobe") and eosinophilic cytoplasm with perinuclear clearing. The nuclear outline was irregular and wrinkled. Nucleoli were inconspicuous and mitotic figures were barely seen. Hale colloidal iron stain was positive in all cases. Immunohistochemically, the tumor cells were variably positive for EMA (100% ,42/42), CK7 ( 95. 2% ,40/42) , Ksp-cad ( 92. 9% , 39/42 ), CK18 (88. 1% ,37/42), CD117 (61.9,26/42) , CD10 (31. 0% , 13/42) and PAX2 (28. 6% , 12/42). They were negative for vimentin, CA IX and TFE3. The follow-up period in 31 patients ranged from 2 to 77 months (average duration =29 months). Three patients died of tumor metastasis 3,8,13 months respectively after the operation. Twentyeight patients were still alive without evidence of tumor recurrence. Conclusions ChRCC predominantly occurs in middle-aged and elderly patients. It often carries a favorable prognosis. The presence of plant celllike morphology, pale cells with uniform reticular microvesicular appearance and perinuclear clearing are characteristic histologic features. The diffuse positivity for Hale colloidal iron stain and EMA/CK7/Kspcadherin/CD117-positive immunoprofiles are also useful in differential diagnosis. Source

Yu H.,Chinese PLA General Hospital | Yang J.,Chinese PLA General Hospital | Jiao S.,Chinese PLA General Hospital | Li Y.,Chinese PLA General Hospital | And 2 more authors.
International Journal of Clinical and Experimental Pathology

Purpose: T-box transcription factor 21 (T-bet) is a key lineage-defining transcription factor. The purpose of this study was to verify the relationship between T-bet expression in primary tumors and prognosis of breast cancer. Methods: T-bet protein expression was immunohistochemically detected on surgically-obtained tumor samples of 130 (stage I-III) invasive breast carcinomas from Chinese subjects, who were followed up for a mean time of 112 months. Results: T-bet was expressed in the nuclei and cytoplasm of both tumor cells and tumor-infiltrating lymphocytes. In LOG-RANK analysis, higher density of interstitial T-bet+ interstitial lymphocytes was related with longer distant disease-free survival (DDFS) (P = 0.047); higher tumor nuclei T-bet expression was related with shorter DFS (P = 0.021) and DDFS (P = 0.026). Cox multivariate analysis showed that density of interstitial T-bet+ interstitial lymphocytes was an independent positive prognostic factor for DFS (HR = 0.474, P = 0.051) and DDFS (HR = 0.414, P = 0.030); tumor nuclei CTLA-4 expression was an independent adverse prognostic factor for DFS (HR = 3.007, P = 0.003), DDFS (HR = 2.931, P = 0.005) and OS (HR = 2.352, P = 0.029). Conclusions: This study found that, high tumor nuclei T-bet expression in primary tumors of breast cancer was correlated with poor prognosis and high density of T-bet+ interstitial lymphocytes in primary tumors of breast cancer were correlated with favorable prognosis. Source

Zhao L.,401 Hospital of PLA | Zhang S.,Peking Union Medical College | An F.,401 Hospital of PLA | Ma S.,401 Hospital of PLA | And 4 more authors.
International Journal of Clinical and Experimental Medicine

Water soluble chitosan (WSC), with low molecular weight, has many special biological, chemical, and physical properties, such as antifungal activity, antibacterial activity, and antitumor activity. In the current study, we examined its antitumor activity in mouse liver cancer H22-bearing mice. Here, oral water-soluble chitosan was administered to reduce the tumor growth and final tumor weight in liver tumor H22-bearing mice. The results suggested that oral administration of WSC could significantly reduce the tumor size and tumor weight of H22-bearing mouse. WSC also increased CD3+ and CD4+ T cell number, CD4/CD8 ratio and NK cells compared with control group. Some chemokines and cytokines, such as IL-1β, IL-2 and TNF-α levels were also increased compared with the control group. The results indicate that oral administration of WSC can enhance the immunity against tumor proliferation in vivo, and therefore oral administration of WSC may be a potential and promising adjuvant therapy against liver cancer. © 2016, E-Century Publishing Corporation. All rights reserved. Source

Liu Y.,401 Hospital of PLA | Wu H.,General Hospital of Bei Jing Command of PLA | Zhu Y.,401 Hospital of PLA | Gao Y.,401 Hospital of PLA
International Journal of Clinical and Experimental Medicine

Objective: Abundance of evidence implicated that leptin may play a decisive role in cancer occurrence, but the reported results varied across the individually published studies. The objective of this study is to access to what extent the extensively studied -2548G/A polymorphism of LEP gene acts on the onset of multiple cancers. Methods: Eligible studies included in this meta-analysis were identified electronically in PubMed and Embase, and manually in relevant literature. Crude odds ratio (OR) with corresponding 95% confidence interval (CI) was calculated to estimate the risk of cancer associated with the -2548G/A polymorphism. Results: 12 association studies with a total of 5,618 cancer cases and 6,509 healthy controls were pooled into this meta-analysis. The results revealed that compared with the G allele, the A allele was associated with modestly increased risk of overall cancer (OR, 1.21; 95% CI, 1.02-1.44). Following further stratified analyses, a borderline association was indicated in prostate cancer (OR, 1.18; 95% CI, 1.00-1.39), breast cancer (OR, 1.11; 95% CI, 1.00-1.22) and Caucasians (OR, 1.19; 95% CI, 1.00-1.41). Conclusions: This meta-analysis reveals that the A allele of -2548G/A polymorphism may be a determinant of cancer development. © 2014, E-Century Publishing Corporation. All rights reserved. Source

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