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Zhang S.,Southern Medical University | Qing Q.,Sun Yat Sen University | Wang Q.,401 Hospital of Chinese PLA | Xu J.,Southern Medical University | And 4 more authors.
Inflammatory Bowel Diseases | Year: 2013

Background: Syndecan-1 (SDC1) and its endo-beta-D-glucuronidase heparanase (HPA) are implicated in the maintenance of intestinal barrier function, but their detailed functions in Crohn's disease (CD) are not fully investigated. The aim of this study was to determine alteration patterns of SDC1 and HPA and their potential roles in evaluating disease activity and differentiating CD from intestinal tuberculosis (ITB). Methods: Tissue and serum specimens were obtained from 89 patients, including 15 patients with functional bowel disorders, 18 active patients with ITB, and 56 patients with CD (remission = 19, active = 37). Basic clinical data were collected and routine blood tests were analyzed. SDC1 and HPA were measured by immunohistochemistry, enzyme-linked immunosorbent assay, reverse transcriptase polymerase chain reaction, and western blot. Colonic epithelial cells were incubated with recombinant HPA, tumor necrosis factor alpha (TNF-a), and mycobacterium tuberculosis culture filtrate protein to detect the alterations of SDC1 and HPA. Results: In the CD group, SDC1 was significantly decreased in mucosa and increased in serum, whereas HPA level in both were elevated. Such alterations were associated with clinicopathological features representing disease activity and injury severity and were not available in functional bowel disorder and ITB groups. Recombinant HPA incubation increased soluble SDC1 in culture supernatants (P = 2 × 10-4), and low-dose TNF-a effectively enhanced HPA's activity (P = 3 × 1026). Exogenous TNF-a destroyed cellular SDC1 and raised HPA expressions dose dependently, whereas mycobacterium tuberculosis culture filtrate protein showed no effects. Conclusions: Unique alterations of SDC1 and HPA are shown in both patients with CD and in vitro model. The results indicate SDC1 and HPA are potential markers for CD in evaluating its disease activity and differentiating it from ITB. Copyright © 2013 Crohn's & Colitis Foundation of America, Inc. Source


Zhao J.-Z.,Qingdao University | Song Z.-Z.,401 Hospital of Chinese PLA | Jian H.,401 Hospital of Chinese PLA | Peng X.,401 Hospital of Chinese PLA | And 3 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: There are many risk factors for venous thromboembolism (VTE) following total hip replacement, which involves several clinical subjects. There remains no systematic and reliable evaluation system. Frequently, Doppler ultrasound has been used to identify VTE. Acute physiology and chronic health evaluation (APACHE II) has been widely used for severity degree and prognosis evaluation of ICU patients. OBJECTIVE: To evaluate the correlation between APACHE II scoring and VTE after total hip replacement. METHODS: APACHE II scores (preoperative, VTE and pulmonary thromboembolism, PTE) of 98 patients (106 hip joints) who were treated by total hip replacement in the 401 Hospital of Chinese PLA from 2000 to 2005 were analyzed retrospectively. The APACHE II scores were compared between the VTE group and non-VTE group, as well as PTE group and non- PTE group. RESULTS AND CONCLUSION: There were no significances in APACHE II scores between VTE group and non-VTE group (P > 0.05). The APACHE II scores were significantly greater in PTE group compared with non-PTE group (P < 0.05). Results show that there is no correlation between APACHE II and VTE in early stage; but the APACHE II scores were correlated with PTE following VTE. Moreover, the higher the APACHE II scores, the greater risk for PTE is. Source

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