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News Article | October 31, 2016

Digital pathology refers to the process of using computer technology to convert an analog microscopic image into a digital image. A digital pathology system is an image based healthcare information system which creates, manages, stores, shares, analyzes and interprets the digital image through glass slides by using computer technology. Digital pathology helps in accurate diagnosis by standardizing test interpretation and workflow. Digital pathology systems are used in hospitals, research institutions, medical universities and government organizations. The application based market of digital pathology includes human and animal pathology. Digital pathology systems can be integrated with laboratory information system that help to capture, store, manage, share and display patient’s related information such as patient’s case history, case reports and other related documents for the treatment of patients. A digital pathology system helps to reducing laboratory expenses and improving patients care through integration. North America, followed by Europe, has the largest market for digital pathology systems due to reimbursement policies, rise in incidence of chronic diseases and increasing adoption of digital pathology systems in pharmaceutical and biotechnological companies in the region. Asia is expected to show high growth rate in the digital pathology systems market in next few years due to government initiatives and rise in awareness about digital pathology and its benefits in the region. Technological advancement, government initiatives to use of digital pathology to improve the quality of cancer diagnosis, rise in incidence of chronic diseases and increasing development of personalized medicines are some of the key factors driving the global digital pathology systems market. In addition, increasing demand for quality healthcare services and improvement in operational efficiency and productivity are expected to drive the market for digital pathology. However, strict government regulations and high implementation cost involved are some of the key factors restraining the growth for global digital pathology systems market. Growing demographics and economies in the developing countries such as India and China are expected to offer good opportunities for digital pathology systems market in Asia. In addition, increasing demand for powerful IT systems is expected to offer new opportunity to global digital pathology systems market. However, standardization and interoperability issue is a challenge for digital pathology systems market. Shifting focus towards patient-centric approach is one of the major trends that have been observed for global digital pathology systems market. Some of the major companies operating in the global digital pathology systems market are 3DHISTECH Ltd., Hamamatsu Photonics K.K., Leica Microsystems, Ventana Medical Systems, Inc., Definiens, Indica Labs, Mikroscan Technologies, Inc., Visiopharm, Corista and Nikon Instruments Inc.

Micsik T.,Semmelweis University | Kiszler G.,3DHISTECH Ltd. | Szabo D.,3DHISTECH Ltd. | Krecsak L.,Podmaniczky u. 63 | And 4 more authors.
Pathology and Oncology Research | Year: 2015

HER2-positive breast cancers usually benefit from anti-HER2 therapy, thus, HER2 evaluation became inevitable for patient selection. HER2-negative (IHC 0, 1+) and strong positive (IHC 3+) cases can easily be interpreted with immunohistochemistry, but equivocal (IHC 2+) cases require further analysis of HER2 gene amplification using in situ hybridization. Our study aimed to validate digital pathology and automated image analysis for unbiased evaluation of HER2 immunostains. We developed an image segmentation algorithm for analyzing HER2-immunostaining (4B5 clone) in tissue microarrays of breast cancers. Two pathologists assessed 309 microscopic regions of at least 100 tumor cells each—representing all HER2 positivity groups—according to international guidelines either semi-quantitatively or by using the MembraneQuant software. Scoring results were statistically correlated with each other and with FISH data, and almost perfect agreement was found (inter-method Cohen’s kappa = 0.872, Spearman-rho = 0.928). When clinical relevance (scoring disagreement that may define erroneous treatment selection) was examined high agreement was found (quadratic weighted kappa = 0.967). Image analysis classified cases with excellent correlation with visual evaluation, therefore, MembraneQuant software proved to be a reliable tool for assessing HER2 immunoreactions and supporting better targeting anti-HER2 therapy. As digital analysis of immunomorphological markers allows permanent archiving, standardization and accurate reviewing of results, it supports quality assurance initiatives in diagnostic pathology—especially of equivocal cases which are hard to interpret. © 2015, Arányi Lajos Foundation.

Kiszler G.,University of Pécs | Kiszler G.,3 DHISTECH Ltd | Varhalmi E.,University of Pécs | Krecsak L.,Eötvös Loránd University | And 3 more authors.
Invertebrate Survival Journal | Year: 2016

The presence of γ-aminobutyric acid (GABA) immunoreactive elements was observed in the sensory system of Eisenia fetida (Annelida, Clitellata). Among the primary sensory cells a high number of labelled cells was found in the epithelium. Using whole-mount preparations and multispectral recording, the number and the distribution pattern of the immunopositive cells were determined in different body segments. Our morphological analysis revealed four typical types of stained primary sensory cells, which could be responsible for the different role of the GABA mediated sensory functions. The peripheral processes of the primary sensory cells ramifying at the border of epithelium and muscular layer produced a basiepidermal network where GABA-positive fibres and their connections were observed. The central processes of the labelled cells projected directly to the ventral nerve cord (VNC) through the segmental nerves to form the ventrolateral and ventromedial sensory longitudinal axon bundles (SLABs). Inhibitory GABA sensory inputs could influence indirectly the activity of the giant motoneurons through the ventrolateral giant axons, and thus the contraction of the body organizing withdrawal and escape reflexes. Applying ultrastructural investigations the synaptic connections of GABA-immunoreactive structures were identified both in the basiepidermal plexus, in the segmental nerves, and in the SLABs of VNC suggesting multistep regulatory effect of GABA in sensory processing of earthworms. © 2016, Universita degli Studi di Modena e Reggio Emilia. All Rights reserved.

Hegedus M.,3DHISTECH Ltd. | Varga V.S.,3DHISTECH Ltd. | Molnar B.,Hungarian Academy of Sciences
IFMBE Proceedings | Year: 2011

High-resolution scanning of the slides can be optimized based on tissue localization using low-resolution overview images in digital microscopy. Automatic preview image processing and tissue mapping can fail due to lack of sufficient image contrast in case of samples without visible dyes, e.g. for fluorescent samples or for samples prior to staining. We investigated the use of alternative imaging/illumination solutions (e.g. phase contrast, Schlieren, dark field). After examination of the Mie light scattering properties of tissue samples we developed and tested a method for the dark field preview illumination of glass slides based on our results. This solution proved to be well suited even for hydrated tissue samples prior to coverslipping and staining of any kind. Optimization of the staining procedure is also possible based on these overview images of the sample. Nevertheless, in the case of archived and contaminated slides tissue, dust and fingerprints were imaged with equal intensities. Therefore we proposed an off-axis thin line dark field illumination. This approach makes the separation of the images from different planes along the optical axis possible. Thus light scattered on the bottom of the glass slide, on the sample plane and on the surface of the coverslip is projected onto different pixels of the camera sensor and can be discriminated. We designed a compact light source and control program for testing this design and showed how the image of the tissue sample can be reconstructed from a series of pictures with minimizing the effect of contamination artifacts. © 2011 Springer-Verlag Berlin Heidelberg.

Fonyad L.,Semmelweis University | Gerely L.,3DHISTECH Ltd. | Cserneky M.,Semmelweis University | Molnar B.,3DHISTECH Ltd. | And 2 more authors.
Diagnostic Pathology | Year: 2010

Background: The spreading of whole slide imaging or digital slide systems in pathology as an innovative technique seems to be unstoppable. Successful introduction of digital slides in education has played a crucial role to reach this level of acceptance. Practically speaking there is no university institute where digital materials are not built into pathology education. At the 1st. Department of Pathology and Experimental Cancer Research, Semmelweis University optical microscopes have been replaced and for four years only digital slides have been used in education. The aim of this paper is to summarize our experiences gathered with the installation of a fully digitized histology lab for graduate education.Methods: We have installed a digital histology lab with 40 PCs, two slide servers - one for internal use and one with external internet access. We have digitized hundreds of slides and after 4 years we use a set of 126 slides during the pathology course. A Student satisfaction questionnaire and a Tutor satisfaction questionnaire have been designed, both to be completed voluntarily to have feed back from the users. The page load statistics of the external slide server were evaluated.Results: The digital histology lab served ~900 students and ~1600 hours of histology practice. The questionnaires revealed high satisfaction with digital slides. The results also emphasize the importance of the tutors' attitude towards digital microscopy as a factor influencing the students' satisfaction. The constantly growing number of page downloads from the external server confirms this satisfaction and the acceptance of digital slides.Conclusions: We are confident, and have showed as well, that digital slides have got numerous advantages over optical slides and are more suitable in education. © 2010 Fónyad et al; licensee BioMed Central Ltd.

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