Dai Q.-Y.,371 Central Hospital |
Yang T.-T.,371 Central Hospital |
Yu F.-F.,371 Central Hospital |
Wang Q.-Z.,371 Central Hospital |
And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: Transforming growth factor-β1 can promote the proliferation and differentiation of intervertebral disc cells, and participate in the repair process after injury. However, whether transforming growth factor-β1 is involved in the intervertebral disc degeneration remains unclear. Objective: To analyze the expression of transforming growth factor-β1 in the degenerative intervertebral disc tissues, and to study the correlation between transforming growth factor-β1 and intervertebral disc degeneration. Methods: Normal intervertebral disc tissues collected from 30 cases and degenerative intervertebral disc tissues collected from 530 cases were researched by hematoxylin-eosin staining, western blot assay and reverse transcription-polymerase chain reaction. The degenerative intervertebral disc tissues were classified in histopathology, and transforming growth factor-β1 expression in different intervertebral disc degeneration tissues was analyzed and compared with that in normal tissues. Results and Conclusion: Pathological diagnose by hematoxylin-eosin staining showed that, degenerative intervertebral disc tissues were classified into 4 pathological grades. Western blot and reverse transcription-PCR showed positive expressions of transforming growth factor-β1 in both normal and degenerative intervertebral disc tissues, however, transforming growth factor-β1 expression levels increased as degenerative changes and showed significant differences between degenerative and normal tissues (P < 0.01). Overexpression of transforming growth factor-β1 is positively correlated with intervertebral disc degeneration in human. Source