324th Hospital of PLA
324th Hospital of PLA
Li J.,324th Hospital of PLA |
Song L.,324th Hospital of PLA |
Yu Z.,324th Hospital of PLA |
Qiao Q.,324th Hospital of PLA |
And 5 more authors.
Chinese Journal of Interventional Imaging and Therapy | Year: 2016
Objective: To explore the morphologic features of the rotator interval (RI) through the comparative study of the thin sectional anatomy based on the Chinese Visible Human (CVH) and MRI sectional anatomy of the RI, and to provide imaging anatomic data for the diagnosis of lesions. Methods: The thin slices of sagittal oblique plane and coronal oblique plane by visualizational reconstruction based on 5 data set of the Chinese visible human (CVH) were compared with the corresponding MR images from 20 shoulder joints of the normal volunteer individuals with routine MR imaging, 5 shoulders without abnormalities with MR arthrography. The detailed sectional anatomy structure of the RI was marked from the CVH, routine MRI and MR arthrography one by one with Photoshop CS2 software. Results: Among the three methods of depicting the RI, the CVH was the most suitable for evaluation of the borders of the RI and was better than MR T1WI and MR arthrography. Including axial, coronal oblique, and sagittal oblique plane, the sagittal oblique images was the most optimal for manifestation of the edges and components of RI. Both the inferior margin of the RI (the scapularis tendon) and the base of the RI (the coracoid process) were the most markedly displayed on the three methods (CVH, MR T1WI and MR arthrography), and on the three of views (axial, coronal oblique, and sagittal oblique plane) of depicting the RI. Conclusion: The CVH has been shown to be the imaging modality of choice for identifying the borders of the RI and its adjacency. The sagittal oblique plane is the best view for displaying the margins, the components and adjacent structures of the rotator interval, which provides helpful position mark for the diagnosis of the lesions in the rotator interval. Copyright © 2016 by the Press of Chinese Journal of Medical Imaging and Technology.
Peng J.,Chongqing Medical University |
Peng J.,324th Hospital of PLA |
Wei K.,Chongqing Medical University |
Zhao X.,Chongqing Medical University |
And 10 more authors.
Molecular Medicine Reports | Year: 2017
Mutant KRAS proto-oncogene GTPase (KRAS) serves an important role in predicting the development, diagnosis, treatment and efficacy of targeted drug therapies for colorectal cancer. To improve the detection efficacy of trace amount of mutant KRAS, the locked nucleic acid-based method was modified in the present study. Internal competitive amplification fragments were used to improve the inhibition of wild-type KRAS with a wild-type blocking (WTB) probe and specifically amplify the trace amounts of mutant KRAS. The modified method, quantitative clamp-based polymerase chain reaction technology using WTB coupled with internal competitive reference to enhance the amplification specificity, named WIRE-PCR, completely blocked the amplification of wild-type KRAS in 50-150 ng DNA templates. The added internal competitive amplified fragments were amplified together with the target gene, which were used to reduce base mismatch due to the high number of cycles in PCR and quantify the total amount of DNA. The results demonstrated that WIRE-PCR facilitated the detection of mutated alleles at a single molecular level. In the colorectal biopsies from 50 patients with suspected colorectal cancer, 18 cases (36%) contained mutant KRAS, and the amount of mutant DNA accounted for 18.6-64.2% of the total DNA. WIRE-PCR is a simple, rapid and low-cost quantitative analysis method for the detection of trace amounts of the mutant KRAS.
Zhao L.,Chongqing Medical University |
Zhao L.,Affiliated Hospital of Zunyi Medical College |
Yang R.,324th Hospital of PLA |
Cheng L.,Chongqing Medical University |
And 3 more authors.
Journal of Surgical Research | Year: 2011
Background: Recent studies have revealed that the epithelial-mesenchymal transition (EMT) of bile duct epithelial cells is engaged in hepatic fibrogenesis. However, the association between etiological factors of liver disease such as virus or bacterial infection and EMT remains to be investigated. The present study focuses on the inductive role of endotoxin, the main component of the cell wall's ectoblast of gram-negative bacteria, in the EMT of human intrahepatic biliary epithelial cells (HIBEpiCs). Methods: The expressions of E-cadherin, S100A4, α-SMA, TGF-β1, and Smad2/3 in HIBEpiCs cultured with or without lipopolysaccharide LPS, were detected by real-time PCR and Western blotting. We blocked the expression of TGF-β1 using paclitaxel and knocked down Smad2/3 by siRNA to explore the role of TGF-β1/Smad2/3 pathway in the EMT of HIBEpiCs. Results: Resting HIBEpiCs showed epithelioid cobblestone morphology, and underwent a phenotypic change to produce bipolar cells with a fibroblastic morphology when co-cultured with LPS. After LPS stimulation and the up-regulation of mRNA and protein expression of TGF-β1 and Smad2/Smad3, the mRNA and protein expression of mesenchymal markers (S100A and α-SMA) increased significantly. Paclitaxel inhibited the mRNA and protein expression of TGF-β 1 in vitro. Knock-down of Smad2/3 by siRNA led to up-regulation of epithelial markers E-cadherin and down-regulation of S100A and α-SMA, indicating a reversal of the EMT. Conclusions: LPS can induce the expression of TGF-ß1 and a subsequent EMT in HIBEpiCs, and the inhibition of TGF-ß1 or Smad 2/3 could reverse this EMT, suggesting that LPS may play a potential role in the EMT of HIBEpiCs. © 2011 Elsevier Inc. All rights reserved.
Lei T.,Chongqing Medical University |
Lei T.,324th Hospital of PLA |
Xie L.,324th Hospital of PLA |
Tu W.,Chongqing Medical University |
And 3 more authors.
Injury | Year: 2012
Objective: In an attempt to explore new tools for constructing a model of blast injuries to the human mandible, a finite element method was used. This model allowed us to perform dynamic simulations and analyse the injury processes and severity of trauma to the human mandible from an explosion striking at the middle mandibular angle. Methods: A 3D finite element model of the human mandible was created using digitally visualised CT scanning data of the human mandible. It was used to dynamically simulate the complete injury process of a blast event to a human mandible (at the middle mandibular angle) under the injury conditions of a 600 mg TNT explosion. The model was also used to elucidate the subsequent mandibular damage and the dynamic distribution of several biomechanical indices (e.g., stress, and strain). The resulting data were subjected to a comparative analysis. Results: Simulation was successfully conducted for injury events in which 600 mg of TNT exploded at 3 cm, 5 cm and 10 cm from the middle mandibular angle of a human mandible; specifically, the simulation included the dynamic injury processes and the distribution of stress and strain in various parts of the damaged mandible. A comparison of the simulation data revealed that different blast distances resulted in considerable variation in the severity and biological indices of the mandibular injury. Conclusion: The finite element model was able to dynamically simulate the blast-initiated trauma processes to a human mandible, which allowed for investigation of the severity of damage to the mandible under different injury conditions. This model and the simulation method are conducive for applications in basic studies and clinical investigations of blast-initiated injury mechanisms of bone tissues. Crown Copyright © 2012 Published by Elsevier Ltd. All rights reserved.
Cui G.Y.,Chongqing Medical University |
Deng C.Y.,324th Hospital of PLA |
Zang L.J.,Chongqing Medical University |
Fei L.,Chongqing Medical University |
Hua F.,Chongqing Medical University
Undersea and Hyperbaric Medicine | Year: 2011
This study aimed to evaluate the effect of hyperbaric oxygen preconditioning (HBO2P) on ischemic metabolites and the glutamate level after permanent middle cerebral artery occlusion (MCAO). HBO2P was administered: five treatments, one treatment per day. The permanent rabbit MCAO models were induced by a modified transorbital approach. The microdialysis procedures were performanced in the right peri-infarct area and contralateral area. MCAO decreased glucose levels while increasing lactate, pyruvate and lactate/pyruvate ratios. Early increase of glycerol and glutamate were also shown. HBO2P stabilized the glucose level and decreased the lactate/pyruvate ratios and glycerol in the peri-infarct area. In addition, it inhibited the increase of the glutamate level. Our study demonstrated that MCAO led to the imbalance of brain energy metabolites and excitatory amino acids. The modulation of energy metabolism and glutamate may be one of the factors contributing to the neuroprotective property of HBO2P. Copyright © 2011 Undersea & Hyperbaric Medical Society, Inc.
Zhou Y.-F.,324th Hospital of PLA |
Zhang F.-X.,324th Hospital of PLA |
Zhang J.-M.,324th Hospital of PLA
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: Neurodegenerative disease is a kind of disease with slow onset, progressive duration and poor prognosis, so far, there is no effective treatment method. Cell transplantation provides a new idea for the treatment of neurodegenerative disease. OBJECTIVE: To observe the trasplated cells, modifiers and methods used in cell transplantation for the treatment of neurodegenerative disease, and to explore whether "transdifferentiation" appeared after cell transplantation. METHODS: Articles related to cell transplantation for the treatment of neurodegenerative disease were collected, and the transplanted cells and modifiers used in cell transplantation for the treatment of neurodegenerative disease were analyzed, as well as the method of cell transplantation for the treatment of Parkinson's disease, Alzheimer's disease and amyotrophic lateral sclerosis. RESULTS AND CONCLUSION: At present, the research on the treatment of neurodegenerative disease are mainly focus on bone marrow stem cells, embryonic stem cells, umbilical cord blood stem cells, neural stem cells and allogeneic cells. The modifiers used in cell transplantation for the treatment of neurodegenerative disease includs tyrosine hydroxylase, orphan nuclear receptor, neurotrophic factor, Von Hippel-Lindau gene, interleukin-1 and melatonin. Cell transplantation can treat Parkinson's disease by transplanting the cells into rat striatum, and cell transplantation can treat Alzheimer's disease and amyotrophic lateral sclerosis by transplanting the cells into the hippocampus region and subarachnoid space. Cell transplantation for the treatment of neurodegenerative disease is becoming more mature, but there is no clear conclusion on whether "transdifferentiation" appears after transplantation.
Wang Z.,Chongqing Medical University |
Zhou J.,Chongqing Medical University |
Luo X.,Chongqing Medical University |
Xu Y.,Chongqing Medical University |
And 4 more authors.
PLoS ONE | Year: 2015
Background: The impact of strabismus on visual function, self-image, self-esteem, and social interactions decrease health-related quality of life (HRQoL).The purpose of this study was to evaluate and refine the adult strabismus quality of life questionnaire (AS-20) by using Rasch analysis among Chinese adult patients with strabismus. Methods: We evaluated the fitness of the AS-20 with Rasch model in Chinese population by assessing unidimensionality, infit and outfit, person and item separation index and reliability, response ordering, targeting and differential item functioning (DIF). Results: The overall AS-20 did not demonstrate unidimensional; however, it was achieved separately in the two Rasch-revised subscales: the psychosocial subscale (11 items) and the function subscale (9 items). The features of good targeting, optimal item infit and outfit, and no notable local dependence were found for each of the subscales. The rating scale was appropriate for the psychosocial subscale but a reduction to four response categories was required for the function subscale. No significant DIF were revealed for any demographic and clinical factors (e.g., age, gender, and strabismus types). Conclusion: The AS-20 was demonstrated by Rasch analysis to be a rigorous instrument for measuring health-related quality of life in Chinese strabismus patents if some revisions were made regarding the subscale construct and response options. © 2015 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Mou L.,324th Hospital of PLA |
Mou L.,Chongqing University |
Wu B.,Chongqing University |
Hu Y.,324th Hospital of PLA |
And 2 more authors.
Cell Biochemistry and Biophysics | Year: 2014
This study aims to observe the expression of the HPV18E2 gene in cervical cancer and premalignant lesions and to investigate its clinical significance. The expression of the HPV18E2 gene in the cervical tissues obtained from 38 women with cervical lesions was detected using the RT-PCR method. The pathological changes were graded based on cervical intraepithelial neoplasia (CIN) criteria. The HPV18E2 gene was expressed mainly in cervical premalignant lesions, 60 % in Grade I CIN, 33.3 % in Grade II CIN, and 28.6 % in Grade III CIN. No expression was detected in cervical cancer and chronic cervical inflammation. This study suggests that peptides vaccine targeting the HPV18E2 protein may disrupt and prohibit the progress of diseases induced by HPV 18 infection (i.e., CIN and cervical cancer). © 2014, Springer Science+Business Media New York.
PubMed | 324th Hospital of PLA
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2014
This study aims to observe the expression of the HPV18E2 gene in cervical cancer and premalignant lesions and to investigate its clinical significance. The expression of the HPV18E2 gene in the cervical tissues obtained from 38 women with cervical lesions was detected using the RT-PCR method. The pathological changes were graded based on cervical intraepithelial neoplasia (CIN) criteria. The HPV18E2 gene was expressed mainly in cervical premalignant lesions, 60 % in Grade I CIN, 33.3 % in Grade II CIN, and 28.6 % in Grade III CIN. No expression was detected in cervical cancer and chronic cervical inflammation. This study suggests that peptides vaccine targeting the HPV18E2 protein may disrupt and prohibit the progress of diseases induced by HPV 18 infection (i.e., CIN and cervical cancer).