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Xian, China

Bi L.,PLA Fourth Military Medical University | Li D.-C.,Ninth Hospital of Xian | Huang Z.-S.,323 Hospital of PLA | Yuan Z.,PLA Fourth Military Medical University
Artificial Organs | Year: 2013

Processed xenegeneic cancellous bone represents an alternative to bone autograft. In order to observe the effects of present prion inactivation treatments on the natural properties of xenogeneic cancellous bones, we treated bovine bone granules with sodium hydroxide (NaOH), sodium hypochlorite (NaOCl), and gaseous hydrogen peroxide (gH2O2) respectively in this study. The microstructure, composition, and mineral content of the granules were evaluated by scanning electron micrograph, energy dispersive X-ray spectroscopy, ash analysis, and micro-computed tomography. The biomechanical property was analyzed by a materials testing machine. The cytocompatibility was evaluated by using a mouse fibroblast cell line (3T3). The microstructure, organic content, and mechanical strength were dramatically altered at the surface of bone in both NaOH- and NaOCl-treated groups, but not in the gH2O2-treated group. Compared with the gH2O2-treated group, attachment and proliferation of 3T3 were reduced in either NaOH- or NaOCl-treated groups. As the consequence, gH2O2 treatment may be a useful approach of disinfection for the preparation of natural cancellous bone with well-preserved structural, mechanical, and biological properties. © 2013, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc. Source

Lv M.-M.,PLA Fourth Military Medical University | Cheng Y.-C.,Third Hospital of PLA | Xiao Z.-B.,323 Hospital of PLA | Sun M.-Y.,PLA Fourth Military Medical University | And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2014

The molecular basis for group I metabotropic glutamate receptors (mGluR1 and 5) coupling to membrane ion channels and intracellular calcium pools is not fully understood. Homer is a family of post synaptic density proteins functionally and physically attached to target proteins at proline-rich sequences. In the present study, we demonstrate that Homer1b/c is constitutively expressed in PC12 cells, whereas Homer1a, the immediate early gene product, can be up-regulated by brain derived neurotrophic factor (BDNF) and glutamate. Knockdown of Homer1b/c using specific target small interfering RNA (siRNA) did not interfere the expression of mGluR1, mGluR5 and their downstream effectors, including inositol-1,4,5-trisphosphate receptors (IP3R), phospholipase C (PLC) and Gq proteins. By analyzing Ca2+ imaging in PC12 cells, we demonstrated that Homer1b/c is an essential regulator of the Ca2+ release from the endoplasmic reticulum (ER) induced by the activation of group I mGluRs, IP3R and ryanodine receptors (RyR). Furthermore, the group I mGluRs activation-dependent refilling of the Ca 2+ stores in both resting and depolarizing conditions were strongly attenuated in the absence of Homer1b/c. Together, our results demonstrate that in PC12 cells Homer1b/c is a regulator of group I mGluRs related Ca2+ homeostasis that is essential for the maintenance of normal Ca2+ levels in the ER. © 2014 Elsevier Inc. All rights reserved. Source

Sun W.,Xijing University | Sun W.,General Hospital of Lanzhou | Qiao Y.,Xijing University | Guo L.,323 Hospital of PLA | And 4 more authors.
Chinese Journal of New Drugs | Year: 2011

Objective: To evaluate the tolerance and safety of recombinant human interferon α1b injection in children with viral pneumonia. Methods: The initial dose and maximal tolerance dose were determined according to preclinical safety data. Children (n=24) in recovery from viral pneumonia were randomly chosen, and intramuscularly injected with single dose and multidoses of the recombinant human interferon α1b injection. During the study, the safety was assessed based on clinical symptoms and laboratory tests. Results: No abnormal outcomes and indicators were found in the physical examination and laboratory blood tests after inhalation, and no serious adverse reactions were observed. Conclusion: The application of recombinant human interferon α1b injection at single doses of 0.3~2.0 μg·kg -1 is safe and tolerable for children in recovery from viral pneumonia. The recommended dosage for phase II clinical trial is 1.5 μg·kg -1. Source

Pu J.,PLA Fourth Military Medical University | Bai D.,323 Hospital of PLA | Yang X.,PLA Fourth Military Medical University | Lu X.,The 323 Hospital of PLA | And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2012

Recently, catecholamines have been described as being involved in the regulation of cancer genesis and progression. Here, we reported that adrenaline increased the cell proliferation and decreased the cisplatin induced apoptosis in HT29 cells. Further study found that adrenaline increased miR-155 expression in an NFκB dependent manner. HT29 cells overexpressing miR-155 had a higher cell growth rate and more resistance to cisplatin induced apoptosis. In contrast, HT29 cells overexpressing miR-155 inhibitor displayed decreased cell proliferation and sensitivity to cisplatin induced cell death. In summary, our study here revealed that adrenaline-NFκB-miR-155 pathway at least partially contributes to the psychological stress induced proliferation and chemoresistance in HT29 cells, shedding light on increasing the therapeutic strategies of cancer chemotherapy. © 2012 Elsevier Inc. Source

Bai D.,PLA Fourth Military Medical University | Gao Q.,PLA Fourth Military Medical University | Li C.,PLA Fourth Military Medical University | Ge L.,Chongqing Medical University | And 2 more authors.
Cellular Signalling | Year: 2012

Persistent fibroblast activation in wound repair is believed to be the key reason for fibrosis and transforming growth factor (TGF)β is considered as one of the key mediators for the fibrogenic response, with the detailed mechanism largely unknown. Here we found that TGFβ1 treatment could induce a significant increase of endogenous TGFβ1 expression by enhancing the mRNA stability in cardiac fibroblasts. Further study revealed that TGFβ1 treatment translocated the nuclear HuR into cytoplasm, which in turn bound the ARE in the 3'UTR of TGFβ1 and increased the mRNA stability as seen from the RNA-IP and reporter assay. Knockdown of HuR decreased the endogenous expression of TGFβ1 under exogenous TGFβ1 treatment, simultaneously with the decrease of Col1a, Col3a and fibronectin expression. Our study here established a TGFβ1/HuR feedback circuit regulating the fibrogenic response in fibroblasts, and targeting this feedback loop is of great potential to control fibrosis. © 2012 Elsevier Inc.. Source

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