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Hanzhong, China

Pang L.,Tianjin Entry Exit Inspection and Quarantine Bureau | Luo Y.,Chinese PLA General Hospital | Gu Y.,3201 Hospital | Xu X.,National Institutes for Food and Drug Control | And 3 more authors.
Microbial Drug Resistance | Year: 2015

The growth of certain methicillin-resistant Staphylococcus aureus (MRSA) isolates could be inhibited by NaCl higher than 2.5%. The objective of this study was to develop an enrichment method to recover NaCl-susceptible MRSA isolates from meat samples. The growth of 12 MRSA and 10 non-MRSA strains was measured in Mueller-Hinton (MH) broth supplemented with 2.5%, 4%, 6.5%, and 7.5% NaCl. Selective agents, including aztreonam, polymyxin B, NaCl, nalidixic acid, and NaN3, were determined for their inhibitory effect to MRSA and non-MRSA strains in MH broth. Based on these data, a two-step enrichment method was developed to recover both NaCl-susceptible and -resistant MRSA isolates in meat products. Comparing to the enrichment method that only used MH broth supplemented with 6.5% NaCl, five additional NaCl-susceptible MRSA isolates were recovered from 92 retail ground pork samples by this newly developed two-step enrichment method. To the best of our knowledge, this is the first study that considers NaCl-susceptible MRSA recovery from ground pork samples. The application of this new enrichment method might expand the diversity of MRSA isolates recovered from various samples. © Copyright 2015, Mary Ann Liebert, Inc. 2015. Source

Wang G.,Chinese Academy of Sciences | Xie G.,Peking University | Jiang T.,3201 Hospital | Wang Y.,Chinese Academy of Sciences | And 5 more authors.
Anesthesia and Analgesia | Year: 2012

BACKGROUND: Bleeding and the need for allogeneic transfusions are still problems after off-pump coronary artery bypass grafting (OPCAB) surgery. We therefore evaluated the effects of an antifibrinolytic, tranexamic acid, on postoperative bleeding and transfusion requirements in patients undergoing OPCAB surgery. METHODS: Two hundred thirty-one consecutive patients scheduled for elective OPCAB were enrolled in the study. Using a double-blind method, the patients were randomly assigned to receive either tranexamic acid (bolus 1 g before surgical incision followed by an infusion of 400 mg/h during surgery; n = 116) or a placebo (infusion equivalent volume of saline solution; n = 115). The primary outcome was 24-hour postoperative chest tube drainage. Allogeneic transfusion, mortality, major morbidities, and resource utilization were also recorded. RESULTS: In comparison with the placebo group, the patients receiving tranexamic acid had a significant reduction in chest tube drainage at 6 hours (270 ± 118 mL vs 416 ± 179 mL, P < 0.001) and 24 hours (654 ± 224 mL vs 891 ± 295 mL, P < 0.001). There was also a significant reduction in allogeneic red blood cell transfusions (47 vs 31.9%, P = 0.019) and fresh frozen plasma (29.6% vs 17.2%, P = 0.027) transfusions. There were no differences in mortality, morbidity, and resource utilization between the 2 groups. CONCLUSIONS: Tranexamic acid reduces postoperative chest tube drainage and the requirement for allogeneic transfusion in off-pump coronary surgery. Copyright © 2012 International Anesthesia Research Society. Source

Xu X.,National Institutes for Food and Drug Control and 2 Tiantan Xili | Cui S.,National Institutes for Food and Drug Control and 2 Tiantan Xili | Zhang F.,National Institutes for Food and Drug Control and 2 Tiantan Xili | Luo Y.,Chinese PLA General Hospital | And 8 more authors.
Microbial Drug Resistance | Year: 2014

Retail meat products could serve as an important medium for the transfer of multidrug resistant isolates from food-producing animals to the community. In this study, the prevalence and characteristics of cefotaxime and ciprofloxacin co-resistant Escherichia coli isolates were investigated in retail chicken and ground pork samples from four provinces of China. The isolates were subjected to phylogenetic group typing and antimicrobial susceptibility testing. All isolates were further characterized by pulsed-field gel electrophoresis to determine the genetic relatedness. These isolates were also screened for beta-lactamase genes, quinolone resistance determinants by PCR, and followed by DNA sequence analysis. Cefotaxime and ciprofloxacin co-resistant E. coli isolates with diverse genetic origins were recovered in 31.9% (106/332) of retail meat samples. E. coli isolates of phylogenetic group A were dominant (59.4%, 63/106), and all isolates showed multidrug resistant profiles. The dominant resistant profiles were AMP-CAZ-CTX-CIP-CHL-GEN-SXT-TET (n=43) and AMP-CAZ-CTX-CIP-CHL-SXT-TET (n=43). Point mutations in quinolone resistance determination regions of topoisomerases were identified in all the isolates, and most of the isolates accumulated three (n=78) or four (n=21) point mutations. Plasmid-mediated quinolone-resistant determinants were identified in 68 isolates, including oqxAB (n=66), qnrS1 (n=7), qnrS2 (n=4), and aac(6′)-Ib-cr (n=9). Eight subtypes of blaCTX-M were identified in 103 E. coli isolates, and blaCTX-M-55 (n=90) was dominant. This study highlights that retail meat could serve as an important reservoir of cefotaxime and ciprofloxacin co-resistant E. coli isolates. It is necessary to evaluate their contribution in the community and hospital infections. © Copyright 2014, Mary Ann Liebert, Inc. 2014. Source

Li W.-T.,3201 Hospital | Li H.,3201 Hospital | Wei J.,3201 Hospital
International Journal of Ophthalmology | Year: 2010

• AIM: To compare the visual refractive outcome and complication of laser in situ keratomileusis (LASIK) treated with 5g/L loteprednol etabonate and 1g/L fluorometholone ophthalmic suspension after operation. • METHODS: Totally 160 consecutive eyes that received 5g/L loteprednol etabonate ophthalmic suspension (group A) and 1g/L fluorometholone ophthalmic suspension (group B) after LASIK were reviewed. Manifest refraction, uncorrected visual acuity (UCVA), best spectacle-corrected visual acuity (BSCVA), and intraocular pressure (IOP) were recorded before and 1 day, 1 week, 1 month, 3 months after treatment. Diffuse lamellar keratitis (DLK), postoperative complications, and frequency of retreatments were observed. • RESULTS: There was no difference in manifest refraction, UCVA, BSCVA and DLK after operation between two groups. Preoperatively IOP didn't differ significantly between two groups. IOP in group A and B were 13.3 ± 3.5mmHg and 16.4 ± 4.9mmHg respectively 3 months after operation. IOP in group A was lower significantly than the later. • CONCLUSION: Loteprednol etabonate ophthalmic suspension 5g/L can suppress the inflammation response caused by LASIK effectively. Compared with traditional steroid suspension, 5g/L Etabonate ophthalmic suspension can obviously reduce occurrence of high IOP. Source

Li R.,3201 Hospital | Ma W.,3201 Hospital | Zhang S.,3201 Hospital
Cancer Research and Clinic | Year: 2015

Although the treatment has made great progress in breast cancer, there are still many problems. For example, primary or secondary on chemotherapy drug resistance and the limitation of treatment in triple-negative breast cancer. So, targeted therapy has become a new strategy to improve this situation. Previous studies showed that the occurrence of breast cancer may be related to the activation of PI3K/Akt/mTOR signaling pathways, and mTOR is an index to judge the prognosis. Parts of breast cancers can obtain clinical benefit with mTOR inhibitors, and it might be a potential treatment target in breast cancer. Source

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