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Chen D.,PLA Fourth Military Medical University | Xia J.-L.,PLA Fourth Military Medical University | Jiang Z.-W.,PLA Fourth Military Medical University | Wang Y.-J.,The 309 Hospital of PLA
Chinese Journal of New Drugs | Year: 2012

Randomization is the standard procedure in clinical trials and the equal randomization is a common method with the advantage of the biggest power. Furthermore the equal randomization can not use the accumulating knowledge in the accrual of trial to determine which treatment is better or the trial should be stopped early. The ethics in clinical trials can not accept the randomization that makes the subjects facing the additional risk by worse effect. This paper presented a Bayesian adaptive randomization method to solve these problems. Source


Wang Y.,Bayi Childrens Hospital | Yang X.,Bayi Childrens Hospital | Zheng Y.,The 309 Hospital of PLA | Wu Z.-H.,Beijing Institute of Disease Control and Prevention | And 5 more authors.
PLoS ONE | Year: 2013

Inflammation plays an important role in the etiology and pathophysiology of spontaneous preterm birth (SPTB), and selenoprotein S (SEPS1) is involved in regulating the inflammatory response. Recently the G-105A promoter polymorphism in SEPS1 was shown to increase pro-inflammatory cytokine expression. We examined whether this functional polymorphism was related to the risk of SPTB in a Chinese population. We also examined the impact of premature rupture of membranes (PROM) on susceptibility to SPTB. The SEPS1 G-105A polymorphism was genotyped in 569 preterm singleton neonates and 673 term neonates by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. χ2 tests and logistic regression analyses were used to calculate the odds ratios (ORs) and 95% confidence intervals (95% CIs). We observed that, compared with the GG genotype, -105A positive genotypes (GA + AA genotypes) were associated with significantly increased susceptibility to SPTB (adjusted OR, 1.87; 95% CI, 1.36-2.57; P<0.001). The -105A positive genotypes were also significantly associated with increased susceptibility to SPTB, both in the patients with PROM (adjusted OR, 2.65; 95% CI, 1.73-4.03; P<0.001) and in those without PROM (adjusted OR, 1.56; 95% CI, 1.09-2.24; P = 0.015). The -105A positive genotypes were also significantly associated with increased susceptibility to SPTB between extremely preterm neonates and controls (adjusted OR, 4.46; 95% CI, 1.86-10.73; P = 0.002) and between moderately preterm neonates and controls (adjusted OR, 1.76; 95% CI, 1.25-2.47; P = 0.001). Our findings suggest that the SEPS1 G-105A polymorphism contributes to the risk of developing SPTB in a Chinese population. © 2013 Wang et al. Source


Ma Y.,The 309 Hospital of PLA | Liang X.,The 309 Hospital of PLA | Shi B.,The 309 Hospital of PLA | Zhang C.,Dalian University
International Journal of Clinical and Experimental Medicine | Year: 2016

The aim of this study was to dynamically observe and compare the effects of vacuum-assisted closure (VAC) technology on the healing of seawater-immersed blast-injury wounds (SIBIW) of pig skin soft tissues, and to summarize the mechanisms of VAC technology in promoting such SIBIW healing, with the goal of providing new experimental evidence for the rescue of such wounds. The bilateral scapular regions and hips of 5 small pigs un-derwent SIBIW with detonators. After the debridement, the pigs were randomly divided into control and experiment groups (VAC-120 mmHg group, VAC-180 mmHg group, VAC-240 mmHg group), with 5 wounds in each group. The control group’s dressings were changed daily, and the experiment group’s dressings were changed every other day. All wounds were treated for 9 days, and were observed until they had all healed (i.e., on the 58th day). We then compared and observed the macroscopic and microscopic changes in the wounds of each group. Compared with the control group, the experimental groups showed more active proliferation of peri-wound cells, and faster crawling of the epithelium. In the early stage, the expression of type I collagen was low, while that of type III collagen was higher, the bacterial index decreased rapidly, the bacterial transition was faster, and the wound healed faster. In treating SIBIW, VAC technology had obvious advantages over conventional therapy. © 2016, E-Century Publishing Corporation. All rights reserved. Source


Zhang L.,The 309 Hospital of PLA | Hou Y.-H.,The 309 Hospital of PLA | Wu K.,The 309 Hospital of PLA | Zhai J.-S.,The 309 Hospital of PLA | Lin N.,The 309 Hospital of PLA
World Journal of Gastroenterology | Year: 2010

AIM: To investigate and elucidate the molecular mechanism underlying varioliform gastritis for early detection, prevention and intervention of gastric cancer. METHODS: A combination of two-dimensional gel electrophoresis and mass spectrometry was used to detect the differentially expressed proteins between varioliform gastritis and matched normal mucosa. The selected proteins were confirmed by Western blotting and reverse transcription polymerase chain reaction (RT-PCR) in additional samples and the function of some proteins in varioliform gastritis was analyzed by bio-method preliminarily. RESULTS: We identified 21 differentially expressed proteins in varioliform gastritis, and compared them with matched normal mucosa. Eleven proteins were upregulated and ten downregulated in varioliform gastritis when compared with the same proteins in individualmatched normal gastric mucosa. These proteins are related to metabolism, oxidation, cytoskeleton, apoptosis, signal transduction and other aspects of cells. Two novel proteins, thioredoxin domain-containing protein 5 (TXNDC5) upregulated in varioliform gastritis, and neuropolypeptide h3 [phosphatidylethanolamine-binding protein 1 (PEBP1)] downregulated in varioliform gastritis, were further investigated. Their expressions were validated by Western blotting and RT-PCR in 12 cases of varioliform gastritis which was matched with normal mucosa. The expression level of PEBP1 in varioliform gastritis was significantly lower (P < 0.05) while that of TXNDC5 was significantly higher than that in matched normal gastric mucosa (P < 0.05). CONCLUSION: There are some changes of protein expression in varioliform gastritis. Downregulation of PEBP1 and upregulation of TXNDC5 are involved in the development of varioliform gastritis. © 2010 Baishideng. Source

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