309 Hospital of Chinese PLA

Beijing, China

309 Hospital of Chinese PLA

Beijing, China
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Mizandari M.,Tbilisi State Medical University | Pai M.,Imperial College London | Xi F.,Imperial College London | Valek V.,University Hospital Brno Bohunice | And 12 more authors.
CardioVascular and Interventional Radiology | Year: 2013

Purpose: Previous clinical studies have shown the safety and efficacy of this novel radiofrequency ablation catheter when used for endoscopic palliative procedures. We report a retrospective study with the results of first in man percutaneous intraductal radiofrequency ablation in patients with malignant biliary obstruction. Methods: Thirty-nine patients with inoperable malignant biliary obstruction were included. These patients underwent intraductal biliary radiofrequency ablation of their malignant biliary strictures following external biliary decompression with an internal-external biliary drainage. Following ablation, they had a metal stent inserted. Results: Following this intervention, there were no 30-day mortality, hemorrhage, bile duct perforation, bile leak, or pancreatitis. Of the 39 patients, 28 are alive and 10 patients are dead with a median survival of 89.5 (range 14-260) days and median stent patency of 84.5 (range 14-260) days. One patient was lost to follow-up. All but one patient had their stent patent at the time of last follow-up or death. One patient with stent blockage at 42 days postprocedure underwent percutaneous transhepatic drain insertion and restenting. Among the patients who are alive (n = 28) the median stent patency was 92 (range 14-260) days, whereas the patients who died (n = 10) had a median stent patency of 62.5 (range 38-210) days. Conclusions: In this group of patients, it appears that this new approach is feasible and safe. Efficacy remains to be proven in future, randomized, prospective studies. © 2012 Springer Science+Business Media New York and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE).


Xing Q.,309 Hospital of Chinese PLA | Huang Z.,309 Hospital of Chinese PLA | Zhang J.,Henan University
Journal of Traditional Chinese Medicine | Year: 2012

OBJECTIVE: The aims of this study were to determine the anti-inflammatory and analgesic effects of Yaotuitong (translation: low back and leg pain) capsules, a Chinese herbal preparation, and the histological changes it induces in experimental rats with chemically induced radicular neuritis. METHODS: Wistar rats were randomly divided into normal, model, Western medicine, and traditional Chinese medicine groups (n=24 per group). We surgically duplicated a chemical radicular neuritis model to simulate lumbar intervertebral disc protrusion. Granuloma formation was measured on postoperative days (PODs) 3, 7, 14, and 21. Prostaglandin E2 and 5-hydroxytryptamine (inflammation mediators) levels in the surrounding tissue and the histology of the nerve root were determined on PODs 7 and 14. RESULTS: Yaotuitong capsules significantly reduced prostaglandin E2 (P<0.01) and 5-hydroxytryptamine (P<0.01) levels in tissue surrounding the nerve root. It also inhibited granuloma formation (P<0.05). CONCLUSION: Yaotuitong capsules have anti-inflammatory and analgesic effects that can alleviate the discomfort of lumbar intervertebral disc protrusion. © 2012 JTCM. All rights reserved.


Xing Q.-C.,309 Hospital of Chinese PLA | Hao L.-B.,Chinese PLA General Hospital | Wang J.-F.,Chinese PLA General Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Staphylococcal infections and its biofilm formation can occur when orthopedic implants or wound is healing, and are regulated by bacterial population sensing mechanism. RNAIII inhibiting peptide intervenes the quorum-sensing system of staphylococcal and blocks the signal transduction among staphylococcal cells, and inhibits staphylococcal biofilm formation, and then prevents staphylococcal infections. OBJECTIVE: To investigate the influence of RNAIII inhibiting peptide on the adhesion of staphylococcus epidermis to the Hela cells. METHODS: The Hela cells were cultured in vitro. There were four groups in this study. In the blank group, saline with dimethyl sulfoxide was added in each well. In the RNAIII inhibiting peptide group, dimethyl sulfoxide solution containing RNAIII inhibiting peptide was added. In the levofloxacin group, levofloxacin was added. In the combination group, the dose was in accordance with above methods. Using intergroup control method, the adhesion of staphylococcus epidermis to the Hela cells was compared under the effects of saline, RNAIII inhibiting peptide and levofloxacin and their combination. RESULTS AND CONCLUSION: In the blank group, abundant bacterial adhered to Hela cells. The number of adhered bacteria was significantly lower in each medicine group than in the blank group (P < 0.001). The spot count was significantly lower in the levofloxacin group than in the RNAIII inhibiting peptide group (P < 0.05). In the combination group, the number of bacteria adhered to Hela cells was decreased (P < 0.01). Results verified that RNAIII inhibiting peptide effectively suppressed the adhesion of staphylococcus epidermis to the host cells, and showed synergistic effects on antibiotics. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Bao D.,309 Hospital of Chinese PLA | Ma Y.-Z.,309 Hospital of Chinese PLA | Chen X.,309 Hospital of Chinese PLA
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Anterior cervical discectomy and intervertebral disc replacement have been extensively used in treatment of cervical degenerative disease. Its short-term therapeutic effects are encouraging, but it lacks of long-term and comprehensive evaluation. OBJECTIVE: To observe the follow-up results of a group of cases of anterior cervical discectomy and Mobi-C intervertebral disc replacement for more than 5 years. METHODS: A total of 25 cases of cervical spondylosis were treated in the 309 Hospital of Chinese PLA from January to September 2009. 19 of them were followed up. All patients received anterior cervical discectomy and Mobi-C intervertebral disc replacement. Twenty-three Mobi-C intervertebral disc prostheses were implanted. The range of motion was measured using lateral X-ray films during extension and flexion. Ectopic ossification was assessed by McAfee method. According to the cervical vertebra MRI images, the degeneration of adjacent segments was determined using Pearce classification. NDI score was used to evaluate the function of cervical spine. Pain improvement was evaluated using visual analogue scale score. RESULTS AND CONCLUSION: A total of 19 patients were followed up for 59_65 months, averagely 62 months. No significant difference in range of motion was detected before surgery and during final follow-up (P > 0.05). Ectopic ossification in two cases and the degeneration of adjacent segments in one case were found during final follow-up. No prosthesis loosening or displacement appeared. Visual analogue scale score and NDI score were significantly lower during final follow-up compared with that before surgery (P < 0.05). These results indicate that under the premise of reasonable choice of indications, the therapeutic effect of Mobi-C intervertebral disc replacement for degenerative cervical spondylosis was satisfactory in five-year follow-up. The range of motion was good, and the incidences of ectopic ossification and the degeneration of adjacent segments were low. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Hu R.,309 Hospital of Chinese PLA | Song L.-J.,309 Hospital of Chinese PLA | Yang Y.,309 Hospital of Chinese PLA | Li T.-H.,309 Hospital of Chinese PLA | Ma H.-J.,309 Hospital of Chinese PLA
Journal of Clinical Dermatology | Year: 2013

A case of primary multiple miliary osteoma cutis is reported. A 25-year-old female presented with a 5 year history of light-yellow papules in zonal distribution on her parietal scalp. The lesions gradually increased in number and spread to the forehead and lower eyelids. Histopathological study of biopsy showed multiple ossification and calcification foci as well as osteocytes in the dermis. The diagnosis of primary multiple military, osteoma cutis was made. The differential diagnosis includes comedones, sebaceous nevus, plaque-like osteomas cutis and cutaneous calcinosis.


Du G.-S.,309 Hospital of Chinese PLA | Hong L.,309 Hospital of Chinese PLA | Shi B.-Y.,309 Hospital of Chinese PLA | Song J.-Y.,309 Hospital of Chinese PLA | And 4 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: Hepatic myelopathy results from liver disease, which lacks of effective cure method. Liver transplantation has attempted to cure this disease; however, the long-term therapeutic effect is rarely reported. OBJECTIVE: To explore the long-term therapeutic effect of liver transplantation in patients with hepatic myelopathy. METHODS: The clinical data of 2 patients with hepatic myelopathy, who underwent orthotopic liver transplantation, in August 2002 and November 2004, at the 309 Hospital of Chinese PLA, were analyzed retrospectively. The time of follow-up was 18 and 43 months, respectively. The muscle strength of double lower limbs in 2 patients was assessed prior to and after operation. RESULTS AND CONCLUSION: Two patients recovered well at 4 weeks after transplantation, the clinical symptom and physical signs of patients were improved obviously, the blood routine examination and other biochemical index were normal, and the function of transplanted kidney was normal. Two patients discharged at 6 weeks after transplantation. Patient 1 could stand for a long time at months 6 after transplantation, walked slowly with the supporter after 12 months and without the supporter at 43 months. The muscular strength of two lower limbs was grade 4. And the liver function was normal. Patients 2 could move his lower limbs in bed at months 6 after transplantation, walked with the supporter at 18 months. The muscular strength of two lower limbs was grade 3. The liver function was normal. It demonstrated that liver transplantation is beneficial to control hepatic myelopathy and recover muscular strength of two lower limbs. It is a newly developed, effective curing method for treating hepatic myelopathy. However, the numbers were small with short time observation, thus, the long-term therapeutic effect still need to be explored.


Qiang W.,309 Hospital of Chinese PLA | Ming C.,309 Hospital of Chinese PLA | Shi B.-Y.,309 Hospital of Chinese PLA | Qian Y.-Y.,309 Hospital of Chinese PLA | And 4 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: Pancreas-kidney transplantation is an effective treatment for diabetes combined with final stage renal disease. However, as the patients suffer diabetes for a long period of time, and cardiovascular system disease is complex, pre- and post-transplantation treatment is very important for successful pancreas-kidney transplantation. OBJECTIVE: To discuss immunosuppressant, coagulant, perioperative and postoperative treatment during pancreas-kidney transplantation to provide some clinical experience for pancreas-kidney transplantation. METHODS: Clinical data of 5 cases undergoing simultaneous pancreas-kidney transplantation in Department of Urinary Surgery, the 309 Hospital of Chinese PLA General Hospital between 2003 and 2008 were retrospectively analyzed to summarize the application of immunosuppressants and anticoagulant drugs and perioperative clinical monitoring focus. RESULTS AND CONCLUSION: There were 5 male patients with an average age of 43 years, and suffered type I diabetes mellitus complicated with final stage renal disease. The preoperative insulin dosage was 1.5-2.4 U/(kg·d). One case had diabetic retinopathy and fundus oculi hemorrhage for many times; two cases showed apparent coronary atherosclerotic heart disease with preoperative cardiac ejection fraction of 52% and 50%. Exocrine of transplanted pancreas had been considered by the intestinal fluid drainage. A total of 3 cases were complete rehabilitation. Of them, 1 case developed acute rejection in the first seven days after operation, but renal function restored with the hormones impact; 1 case had postoperative acute rejection of transplanted duodenum as well as intestinal fistula, eventually, transplanted pancreas was ectomized, but transplanted kidney was preserved; two cases succeeded in restoring and no complications occurred; 1 had postoperative gastrointestinal bleeding and died from multiple organ failure. Simultaneous pancreas-kidney transplantation is the most effective way to treat the diabetes mellitus with terminal nephropathy. Because of complications in the transplanted exocrine pancreas with bladder drainage, it has been replaced by the enteric drainage. Recovery of the transplanted kidney function is important for successful transplantation. After operation, oral FK should be taken when the serum creatinine returned to 300 umol/L. The application of clotting drug is one of the important factors for recovery of transplanted pancreatic function. Jejunostomy is an important therapeutic measure to prevent the reflux of intestinal juice to the transplanted pancreas in perioperative period. In the follow-up period cathartic drugs are recommended to prevent constipation and reduce the occurrence of acute pancreatitis caused by intestinal fluid reflux.


Pang Y.-P.,Hebei North University | Cheng Y.,Chinese PLA General Hospital | Hao H.-J.,Chinese PLA General Hospital | Liu J.-J.,Chinese PLA General Hospital | Shen J.,309 Hospital of Chinese PLA
Medical Journal of Chinese People's Liberation Army | Year: 2015

Objective To evaluate the therapeutic effect of systemic infusion of MSC culture supernatant in STZ-induced diabetic rats, and explore the mechanism of effect of MSC secretion on promoting regeneration of the islet tissues. Methods The diabetic animal model was reproduced in 35 SD rats by intraperitoneal injection of a single large dose of streptozotocin (STZ, 65mg/ kg). The 30 successfully induced diabetic rats were randomly divided into MSC culture supernatant infusion group (CM, n=15) and medium infusion group (M, n=15), and in addition, 15 normal rats were used as control. Animals were intravenously transfused with MSC supernatant (CM group) or raw medium (M group), then the contents of blood glucose were determined 3 days after infusion. The serum insulin and C-peptide levels were monitored and the intraperitoneal glucose tolerance test (IPGTT) was performed on the 7th day of infusion to evaluate the therapeutic effect of MSCs supernatant infusion in diabetic rats. Finally, all the experimental animals were sacrificed at indicated time points and the pancreatic tissues were collected for multiple immunofluorescence staining (MIFS), in order to observe the β-cell regeneration after MSCs supernatant infusion, and to further explore the possible mechanism involved in the experiment. Results At the early stage after infusion (<7 days), the blood glucose level declined and the contents of serum insulin and C-peptide increased obviously in CM group as compared with that of M group (P<0.05). IPGTT showed that the islet function was significantly enhanced in CM group compared with M group. MIFS showed that the number of β cells in the destroyed islets in CM group rats was significantly increased as compared with that of M group rats. In addition, the proliferation rate of β cells was obviously higher in CM group (4%) than in control group (0.5%, P<0.01). Conclusion Treatment with MSCs culture supernatant obviously prompted β cell proliferation in the destroyed islets, resulting in regeneration of the islets with islet function recovery, thus it effectively controls the advance of diabetes. © 2015, People’s Military Medical Press. All rights reserved.


Hu R.,309 Hospital of Chinese PLA | Yang Y.,309 Hospital of Chinese PLA | Song L.-J.,309 Hospital of Chinese PLA | Li T.-H.,309 Hospital of Chinese PLA | Ma H.-J.,309 Hospital of Chinese PLA
Journal of Clinical Dermatology | Year: 2013

A case of bullous pemphigoid caused by levofloxacin is reported. An 82-year-old woman presented with erythema, blister and pruritus for 7 days. The patient had the same lesions after exposure to levofloxacin 6 months ago. The histopathology showed subepidermal blisters, filled with eosinophils. Direct immunofluorescence showed a linear band of IgG and C3 along the basement membrane zone. The diagnosis of levofloxacin induced bullous pemphigoid was made. The patient got satisfactory result after corticosteroid therapy.


Zhang C.,PLA Fourth Military Medical University | Liu Z.,PLA Fourth Military Medical University | Liu Z.,309 Hospital of Chinese PLA | Li Y.,PLA Fourth Military Medical University | And 12 more authors.
Analytica Chimica Acta | Year: 2013

In this study, detection of staphylococcal enterotoxin A (SEA) in multi-matrices using a highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) has been established. A pair of monoclonal antibodies (mAbs) was selected from 37 anti-SEA mAbs by pairwise analysis, and the experimental conditions of the CLEIA were optimized. This CLEIA exhibited high performance with a wide dynamic range from 6.4pgmL-1 to 1600pgmL-1, and the measured low limit of detection (LOD) was 3.2pgmL-1. No cross-reactivity was observed when this method was applied to test SEB, SEC1, and SED. It has also been successfully applied for analyzing SEA in a variety of environmental, biological, and clinical matrices, such as sewage, tap water, river water, roast beef, peanut butter, cured ham, 10% nonfat dry milk, milk, orange juice, human urine, and serum. Thus, the highly sensitive and SEA-specific CLEIA should make it attractive for quantifying SEA in public health and diagnosis in near future. © 2013.

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