307th Hospital of PLA

Fengtai, China

307th Hospital of PLA

Fengtai, China
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Liu Y.,307th Hospital of PLA | Mi L.,Inner Mongolia Agricultural University | Mi Z.,Beijing Institute of Microbiology and Epidemiology | Huang Y.,Beijing Institute of Microbiology and Epidemiology | And 4 more authors.
Genome Announcements | Year: 2016

A novel lytic Salmonella bacteriophage was isolated by using Klebsiella pneumoniae as host cells. The phage's genome was determined to be 47,564 bp and has the highest similarity to Salmonella phage E1 and Salmonella phage 64795_sal3, with coverages of 61% and 56%, respectively. Here, we announce the phage's complete genome. © 2016 Liu et al.


Li P.,307th Hospital of PLA | Huang Y.,Beijing Institute of Microbiology and Epidemiology | Yu L.,Navy General Hospital | Liu Y.,307th Hospital of PLA | And 8 more authors.
International Journal of Infectious Diseases | Year: 2017

Objectives Heteroresistance is a phenomenon in which there are various responses to antibiotics from bacterial cells within the same population. Here, we isolated and characterised an imipenem heteroresistant Acinetobacter baumannii strain (HRAB-85). Methods The genome of strain HRAB-85 was completely sequenced and analysed to understand its antibiotic resistance mechanisms. Population analysis and multilocus sequence typing were performed. Results Subpopulations grew in the presence of imipenem at concentrations of up to 64 μg/mL, and the strain was found to belong to ST208. The total length of strain HRAB-85 was 4,098,585 bp with a GC content of 39.98%. The genome harboured at least four insertion sequences: the common ISAba1, ISAba22, ISAba24, and newly reported ISAba26. Additionally, 19 antibiotic-resistance genes against eight classes of antimicrobial agents were found, and 11 genomic islands (GIs) were identified. Among them, GI3, GI10, and GI11 contained many ISs and antibiotic-resistance determinants. Conclusions The existence of imipenem heteroresistant phenotypes in A. baumannii was substantiated in this hospital, and imipenem pressure, which could induce imipenem-heteroresistant subpopulations, may select for highly resistant strains. The complete genome sequencing and bioinformatics analysis of HRAB-85 could improve our understanding of the epidemiology and resistance mechanisms of carbapenem-heteroresistant A. baumannii. © 2017


Liu H.,307th Hospital of PLA | Zhang P.,Soochow University of China | Xie K.,Tianjin Medical University
NeuroReport | Year: 2017

Postoperative cognitive decline is a major clinical problem with high morbidity and mortality after surgery. Many studies have found that molecular hydrogen (H 2) has significant neuroprotection against acute and chronic neurological injury by regulating inflammation and apoptosis. In this study, we hypothesized that H 2 treatment could ameliorate the development of cognitive impairment following surgery. Adult male rats were subjected to stabilized tibial fracture operation under anesthesia. Two percent of H 2 was inhaled for 3 h beginning at 1 h after surgery. Separate cohorts of rats were tested for cognitive function with fear conditioning and the Y-maze test, or euthanized to assess blood-brain barrier integrity, and systemic and hippocampal proinflammatory cytokine and caspase-3 activity. Surgery-challenged animals showed significant cognitive impairment evidenced by a decreased percentage of freezing time and an increased number of learning trials on days 1, 3, and 7 after operation, which were significantly improved by H 2 treatment. Furthermore, H 2 treatment significantly ameliorated the increase in serum and hippocampal proinflammatory cytokines tumor necrosis factor, interleukin-1β, interleukin-6, and high-mobility group protein 1 in surgery-challenged animals. Moreover, H 2 treatment markedly improved blood-brain barrier integrity and reduced caspase-3 activity in the hippocampus of surgery-challenged animals. These findings suggest that H 2 treatment could significantly mitigate surgery-induced cognitive impairment by regulating inflammation and apoptosis. Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved.


Ma L.,Second Street | Qiu Z.W.,307th Hospital of PLA
European Review for Medical and Pharmacological Sciences | Year: 2014

OBJECTIVE: The aim of this study was to investigate the effect of Magnesium Isoglycyrrhizinate (MgIG) on intercellular adhesion moledule-1 (ICAM-1) and matrix metalloproteinase-9 (MMP-9) in rats with Paraquat (PQ) poisoning and its potential mechanism. MATERIALS AND METHODS: 30 male Sprague Dawley rats were randomly divided into five groups, including normal control group, poisoned control group, low-dose MgIG group, medium-dose MgIG group and high-dose MgIG group. Each group was treated with corresponding dose of MgIG once on daily basis by intraperitoneal injection 24 hours later, and the normal control group and poisoned control group were injected with physiological saline. All the animals were killed 14 days after poisoning, the contents of ICAM-1 and matrix MMP-9 were determined. HE staining and Masson staining were performed, the hydroxyproline (HYP) content in the lung tissue was also determined, and the expressions of ICAM-1 and MMP-9 were detected by immunohistochemical test. RESULTS: The contents of ICAM-1 and MMP-9 in the rat serum for all treatment groups were significantly decreased compared with those of the poisoned control group (p < 0.05, or < 0.01), and the expressions of the two proteins were significantly down-regulated, especially for the medium dose group. CONCLUSIONS: There is an improvement effect of ICAM-1 and MMP-9 in rats with Paraquat poisoning for the medium dose of MgIG, capable of slowing down the process of pulmonary fibrosis to certain extent.


Bai S.,Fifth Hospital of PLA | Wang W.,307th Hospital of PLA | Liu Z.,307th Hospital of PLA
Pharmaceutical Care and Research | Year: 2012

Objective: To investigate the application status and tendency of antibacterials in the 307th Hospital of PLA in order to promote the rational use of antibacterials. Methods: The data of categories, specifications, consumption sum, the frequency of medication(DDDs) of antibacterials in the hospital during 2008-2010 were retrospectively analyzed. Results: The percentage of consumption sum of antibacterials increased slowly and maintained at about 18% these years. The consumption sum of antifungal agents and the DDDs of cephalosporins ranked the first respectively in all antibacterials. Conclusion: The utilization of antibacterials in this hospital was rational in general, but a few categories of antibacterials were used excessively.


PubMed | 307th Hospital of PLA, Beijing Institute of Microbiology and Epidemiology and Inner Mongolia Agricultural University
Type: Journal Article | Journal: Genome announcements | Year: 2016

A novel lytic Salmonella bacteriophage was isolated by using Klebsiella pneumoniae as host cells. The phages genome was determined to be 47,564bp and has the highest similarity to Salmonella phage E1 and Salmonella phage 64795_sal3, with coverages of 61% and 56%, respectively. Here, we announce the phages complete genome.


Yao Y.,General Hospital of PLA | Zhou R.,307th Hospital of PLA | Wang Y.,General Hospital of PLA
Pharmacoepidemiology and Drug Safety | Year: 2012

Purpose: The incidence of adverse reactions to ceftriaxone, which is widely used in China, has gradually increased, with an associated increase in patient fatality. An analysis of the reported data from articles published in China highlights the importance and extent of this growing public health problem. Methods: We identified previously reported cases of adverse effects to ceftriaxone sodium by searching the Chinese Medical Text Database System for reports published between January 2002 and December 2009. The references cited in the articles were examined for additional reports. Results: A total of 17 articles were identified that cited fatal adverse reactions in 22 cases. Most patients had been treated for upper respiratory tract infection and bronchitis and had no reported history of ceftriaxone allergy. Of the patients, 77% were treated with ceftriaxone without undergoing intradermal testing, and 36% were not offered any information on intradermal testing. Concomitant intravenous medications were prescribed in 7 cases, and an overdose of ceftriaxone sodium was prescribed for two patients. The delay between drug administration and the onset of adverse drug reactions occurred within 30min in 72.7% of the patients. Of the deaths, 73% occurred on the first day of treatment. Conclusions: The fatal adverse events associated with ceftriaxone occurred because of inappropriate drug usage, drug overdoses, careless medical personnel, poor medical conditions, and possibly poor drug quality. Although some deaths may be unavoidable, the risks can be minimized by the appropriate administration of ceftriaxone and further consultation with the Chinese medical profession and research. © 2012 John Wiley & Sons, Ltd.


Ren B.,Academy of Military Medical science | Ren B.,307th Hospital of PLA | Zhang Z.-S.,307th Hospital of PLA | Liu W.-W.,Academy of Military Medical science | And 8 more authors.
Journal of Neurosurgery | Year: 2016

OBJECTIVE: Debate exists regarding the merits and shortcomings of an indirect bypass procedure for treating adult patients with moyamoya disease (MMD). Considerable variation in neovascularization occurs among different organs in patients with diabetes mellitus. Here, the effect of encephaloduroarteriosynangiosis on MMD associated with Type 2 diabetes mellitus (T2DM) is evaluated. METHODS: A retrospective and 1:2 matched case-control study was conducted in moyamoya patients with or without T2DM (n = 180). Postoperative collateral formations were graded according to the Modified Collateral Grading System that originated from the Matsushima Angiographic Stage Classification. Neurological function outcomes before and after the operation were evaluated according to the modified Rankin Scale. Univariate and multivariate logistic regression analyses were performed to determine the risk factors for clinical outcome. RESULTS: There was no statistically significant difference in the constituent ratios of initial symptom and preoperative Suzuki stage between patients with and without T2DM. Progression of angiopathy around the circle of Willis was postoperatively observed in bilateral internal carotid arteries in both groups. Patients with T2DM had a higher postoperative Suzuki stage (p < 0.01) and more frequent development of collateral angiogenesis germinating from the external carotid after indirect revascularization procedures in the surgical cerebral hemisphere (82.7% vs 72.2%; p < 0.05). The extent of postoperative collateral formation in patients with diabetes mellitus was significantly higher (p < 0.01). Postoperative clinical improvement in the diabetes group was more common after revascularization procedures (p < 0.05), and the diabetes group had lower modified Rankin Scale scores (p < 0.05) in comparison with the nondiabetes group. Late postoperative stroke and posterior cerebral artery involvement were identified as predictors of unfavorable clinical outcome in both groups, while T2DM was associated with a favorable clinical outcome. CONCLUSIONS: Encephaloduroarteriosynangiosis is an efficacious treatment for adult patients with MMD. Patients with T2DM could achieve better collateral circulation and clinical improvement following surgery. ©AANS, 2016.


Bin Z.,307th Hospital of PLA | Hu C.,307th Hospital of PLA
Chinese Journal of Cancer Biotherapy | Year: 2015

Among the various available cancer treatment modalities, anti-tumor immune escape is attracting tremendous attention worldwide and has become one of the fast-developing research fields, especially when the anti-prostate cancer vaccine was approved by the FDA and the monocloan antibodies, which targeted PD-1 and CTLA-4, were successfully launched into market. Since 2010, tremendous research progress has been made on cellular immunotherapy of cancer in China. Nevertheless, practically cellular immunotherapy of cancer is facing several challenging issues. Including: 1) how to choose the proper treatment modality and time duration; 2) how to manage the quality control; 3) how to evaluate the treatment outcome; and 4) how to obtain stronger empirical evidence to support the benefits of the treatment. With regard to quality management and outcome evaluation, there exist similarities and differences between cellular immunotherapy and traditional biological and chemical drugs for cancers. Similar to the quality control of biological and chemical treatments of cancer, the quality management of cellular immunotherapy of cancer involves such aspects as the structure of the clinic or center, personnel training and testing program. In contrast, the outcome evaluation of cellular immunotherapy of cancer cannot simply follow the traditional method for biological and chemical cancer therapies. This paper aims to review the recent development in the quality management and outcome evaluation of cellular immunotherapy of cancer, hoping to bring these issues to the attention of peers in China. © 2015, Editorial office of Chinese Journal of Cancer Biotherapy. All rights reserved.


PubMed | 307th Hospital of PLA, University of Science and Technology of China and State Key Laboratory of Pathogen and Biosecurity
Type: Journal Article | Journal: Genome announcements | Year: 2016

We report here the first complete genome of Serratia rubidaea, isolated from a patient in China.

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