306th Hospital of PLA

Beijing, China

306th Hospital of PLA

Beijing, China
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Wang Z.,306th Hospital of PLA | Si S.,306th Hospital of PLA | Liu J.,306th Hospital of PLA | Wu Y.,306th Hospital of PLA | And 2 more authors.
Cancer Research and Clinic | Year: 2017

Objective To explore the effect of dose rate of X-rays on migration of non-small cell lung cancer (NSCLC) cells and provide the experimental basis for developing radiotherapy scheme. Methods Human NSCLC cell line A549 was cultured and irradiated with X-rays at dose of 6 Gy from a linear accelerator. The dose rates of 1, 2, 4 and 6 Gy/min were selected. Monolayer adherent cells were scratched and photographed at 0 hour and 24 hours under a microscope to measure the scratch width. Results After 24 hours, the scratch width of nonirradiated control cells was (640.7±8.1) μm. The scratch widths of cells were different when cells were irradiated with X-rays of various dose rates. Scratch widths were the largest in cells irradiated at dose rates of 1 Gy/min [(691.4±7.6) μm] and 6 Gy/min [(691.8±12.1) μm]. The scratch width was (666.2±1.3) μm of X-rays at 4 Gy/min, and there were significant differences compared with nonirradiated group (all P Y 0.01), which suggested that inhibitory effect of X-rays at dose rates on A549 cell migration was obvious. However, the scratch width of cells irradiated at 2 Gy/min [(643.5 ±6.8) μm] had no difference compared with the control cells (t =-0.336, P = 0.742). Conclusions The effect of X-rays irradiation on cell migration of human NSCLC cell line A549 is related with irradiated dose rate. The effect of different dose rates on cell migration is significantly different. Selecting appropriate dose rates for irradiation may help to improve the efficacy of radiotherapy.

Liang W.,Shaoxing Peoples Hospital | Gao B.,306th Hospital of PLA | Fu P.,Tongji University | Xu S.,Shanghai University | And 2 more authors.
Frontiers in Bioscience | Year: 2013

Osteosarcoma is an aggressive sarcoma of the bone characterized by a high level of genetic instability and recurrent DNA deletions and amplifications. MicroRNAs (miRNAs) are non-coding small RNAs, usually 18-25 nucleotides in length, which repress translation and cleave mRNA by base-pairing to the 3 untranslated region of the target genes. miRNAs have demonstrated far-reaching effects on the cellular biology of development and cancer. Their role in osteosarcomagenesis remains largely unexplored. A number of reports have investigated the role of microRNAs in osteosarcoma. This review summeizes the recent research progress of miRNA in the osteosarcome.

Wang B.,Unit No. 92060 of PLA | Pei Z.,306th Hospital of PLA | Wang L.,Peking University
EPL | Year: 2014

The world in which we are living is a huge network of networks and should be described by interdependent networks. The interdependence between networks significantly affects the evolutionary dynamics of cooperation on them. Moreover, due to the diversity and complexity of social and biological systems, players on different networks may engage in different forms of interactions, which should be described by multiple game models in the evolutionary game theory, such as the Prisoner's Dilemma and Snowdrift Game. We therefore study the evolutionary dynamics of cooperation on two interdependent networks playing different games, respectively. We show that, with the increment of network interdependence, the evolution of cooperation is dramatically promoted on the network playing Prisoner's Dilemma, and the cooperation level of the network playing Snowdrift Game reduces correspondingly. Remarkably, there exists an optimal intermediate region of network interdependence maximizing the growth rates of cooperation on the network playing Prisoner's Dilemma. Also on the network playing Prisoner's Dilemma, players contacting with the other network have an advantage in the evolution of cooperation over the other players on the same network, which is contrary to the network playing Snowdrift Game. Copyright © EPLA, 2014.

Zhu T.,Peking University | Pei Z.,Capital Medical University | Huang J.,Peking University | Xiong C.,Peking University | And 3 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2010

In this study, a novel method for detecting bacterial cells in deionized (DI) water suspension is presented by using fluidic electrodes with a hydrodynamic focusing technique. KCl solution was utilized as both sheath flow and fluidic electrodes, and the bacterial suspension was squeezed to form three flowing layers with different conductivities on a microfluidic chip. An impedance analyzer was connected with the KCl solution through two Ag/AgCl wires to apply an AC voltage to fluidic layers within a certain frequency for impedance measurements. Porphyromonas gingivalis and Escherichia coli were detected and linear relationships were found between the impedance and the logarithmic value of the bacterial concentration in certain cell concentration ranges. It is demonstrated that bacterial detection using the microdevice is rapid and convenient, with a chip made of simple flow channels, and the detection sensitivity of cell counting can be tuned by varying the width of the sample flow layer through changing input velocities, showing a detection limit of 103 cells mL-1. © 2010 The Royal Society of Chemistry.

Wang Y.X.,Capital Medical University | Hu L.N.,306th Hospital of PLA | Yang H.,Capital Medical University | Jonas J.B.,Capital Medical University | And 2 more authors.
British Journal of Ophthalmology | Year: 2012

Background: To examine factors associated with progression of open-angle glaucoma in a population-based setting. Methods: The population-based Beijing Eye Study, which included 4439 subjects with an age of 40+ years in the year 2001, was repeated in 2006. Optic disc photographs of the baseline examination versus follow-up examination were compared. Results: Out of 111 open-angle glaucoma patients examined in 2001, 77 (69%) subjects participated in the follow-up examination and 16 (21%) eyes showed glaucoma progression. Glaucoma progression was associated with smaller rim area (p=0.001), larger β zone (p=0.037), higher frequency of β zone increase during follow-up (p=0.01), higher prevalence of disc haemorrhages (p=0.01) and higher single intraocular pressure (p=0.04). In multiple regression analysis, only smaller rim area remained significantly associated with glaucoma progression. Glaucoma progression was not associated with optic disc size (p=0.70), mean blood pressure (p=0.43), ocular perfusion pressure (p=0.96), retinal vessel diameter and retinal microvascular abnormalities (all p>0.10), prevalence of diabetes mellitus (p=0.75) and arterial hypertension (p=0.26), prevalence of dyslipidaemia (p=0.28), refractive error (p=0.69), and central corneal thickness (p=0.97). Conclusions: In a population-based setting in adult Chinese, factors showing an association with open-angle glaucoma progression were an advanced stage of the disease (ie, small rim), presence of disc haemorrhages, larger area of β zone and higher frequency of β zone increase, and higher intraocular pressure. Glaucoma progression was not significantly associated with optic disc size, central corneal thickness, retinal vessel diameter and retinal microvascular abnormalities, and systemic diseases such as diabetes mellitus and arterial hypertension.

Zhou Y.-C.,Xijing University | Liu J.-Y.,Xijing University | Li J.,Xijing University | Zhang J.,Xijing University | And 7 more authors.
International Journal of Radiation Oncology Biology Physics | Year: 2011

Purpose: To examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Methods and Materials: Six cancer cell lines originating from different human organs were irradiated by 60Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. Results: After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. Conclusions: These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells. © 2011 Elsevier Inc.

Li L.Y.,Chinese PLA General Hospital | Liu Q.S.,Chinese PLA General Hospital | Li L.,Chinese PLA General Hospital | Cao Y.J.,306th Hospital of PLA | And 3 more authors.
International Journal of Colorectal Disease | Year: 2011

Purpose The aim of this meta-analysis and systematic review was to determine whether bleeding prophylaxis benefits patients after colonoscopic polypectomy. Methods A systematic review of the literature was performed to find prospective randomized controlled trials of postpolypectomy hemorrhage prophylaxis. Studies were selected according to specific criteria and analyzed to generate pooled data. Results Eight studies encompassing 2,595 polyps met the criteria for the meta-analysis. The rate of early bleeding was significantly decreased relative to the control when a single prophylactic technique was used [2.58% vs. 8.15%, OR= 0.34 (95% CI, 0.20-0.58), P<0.0001]. The use of multiple prophylactic techniques resulted in a lower early postpolypectomy hemorrhage rate compared with the use of a single prophylactic technique [0% vs. 8.41%, OR=0.12 (95% CI, 0.03-0.47), P=0.002]. The late bleeding rate did not differ significantly between the monotherapy and control groups [0.61% vs. 1.39%, OR=0.37 (95% CI, 0.11-1.28), P= 0.12], and the use of combined preventative techniques did not significantly decrease the late postpolypectomy bleeding (PPB) rate compared with use of a single prophylactic method [1.43% vs. 2.05%, OR=0.70 (95% CI, 0.32-1.55), P=0.38]. Conclusion Prophylactic endoscopic treatments are effective at reducing early PPB after colonoscopic polypectomy. However, patients with late PPB may not benefit from bleeding prophylaxis. © Springer-Verlag 2011.

Pei Z.,Capital Medical University | Pei Z.,306th Hospital of PLA | Zhang F.,Capital Medical University | Niu Z.,306th Hospital of PLA | Shi S.,306th Hospital of PLA
Molecular Medicine Reports | Year: 2013

Periodontitis is a common destructive inflammatory disease that leads to changes in the tooth-supporting tissues. Human periodontal ligament cells are essential in periodontal tissue regeneration. The traditional Chinese medicine icariin promoted bone formation, stimulated the osteogenic differentiation of preosteoblastic cells and inhibited osteoclast differentiation and bone resorption. Thus, in the present study, the effect of icariin on cell proliferation and the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL), core binding factor α1 (Cbfa1) and osteocalcin (OC) was investigated in human periodontal ligament cells, by an MTT assay, qPCR and western blot analysis. The results demonstrated that icariin promoted cell proliferation in a dose- and time-dependent manner, upregulated OPG, Cbfa1 and OC expression, and downregulated RANKL production and the RANKL/OPG expression ratio. This suggested the potential value of icariin in treating alveolar bone resorption and promoting periodontal tissue regeneration, due to its ability to stimulate the proliferation and osteogenic differentiation of human periodontal ligament cells and inhibit osteoclast differentiation.

Li H.,China Pharmaceutical University | Mao S.,China Pharmaceutical University | Wang H.,306th Hospital of PLA | Zen K.,China Pharmaceutical University | And 2 more authors.
Protein and Cell | Year: 2014

MicroRNAs (miRNAs) are endogenously expressed small, non-coding transcripts that regulate protein expression. Substantial evidences suggest that miRNAs are enriched in central nervous system, where they are hypothesized to play pivotal roles during neural development. In the present study, we analyzed miRNAs expression in mice cerebral cortex and hippocampus at different developmental stages and found miR-29a increased dramatically at postnatal stages. In addition, we provided strong evidences that miR-29a is enriched in mature neurons both in vitro and in vivo. Further investigation demonstrated that the activation of glutamate receptors induced endogenous miR-29a level in primary neurons. Moreover, we showed that miR-29a directly regulated its target protein Doublecortin (DCX) expression, which further modulated axon branching in primary culture. Together, our results suggested that miR-29a play an important role in neuronal development of mice cerebrum. © 2014 The Author(s).

Si S.,306th Hospital of PLA
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013

To investigate the effects of simulated weightlessness on antitumor immunity of T lymphocytes in mice. The malignant melanoma was xenografted by subcutaneous injection of B16 cells into the right hind limb of every mouse. The mice suspended by tail at a -15 degree to 20 degree head-down tilt were used as simulated weightlessness models. The effects of simulated weightlessness on tumor volume and survival time were observed. T the numbers of leucocytes, lymphocytes and T lymphocyte subsets in peripheral blood of tumor-bearing mice under simulated weightlessness were monitored by an automatic hemacytometer and a flow cytometer. The effects of simulated weightlessness on the production of IL-2, TNF-α and IFN-γ in T lymphocytes and the cytotoxicities of tumor-specific CTLs to tumor cells were analyzed by ELISA and LDH release. Compared with control group, the tumors grew faster, the survival times were shorter, the number of lymphocytes, the ratio of lymphocytes, CD3(+);, CD4(+);/CD3(+); and CD8(+);/CD3(+); T lymphocytes in peripheral blood dropped, and the proliferation of splenic T lymphocytes induced by mitogen was reduced (P<0.05 or P<0.01) in the simulated weightlessness group. The production of IL-2, TNF-α and IFN-γ induced by tumor cells and cytotoxicities of tumor-specific CTLs to tumor cells were inhibited in mice under simulated weightlessness (P<0.05 or P<0.01). Simulated weightlessness inhibits antitumor immunity of T lymphocytes.

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