Nong J.,Research Institute of Transplantation Medicine |
Sun X.-Y.,Research Institute of Transplantation Medicine |
Qin K.,Research Institute of Transplantation Medicine |
Lai Y.-H.,Research Institute of Transplantation Medicine |
And 7 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: Some scholars believe that many factors can lead to pathological changes of the lung in the process of brain-death OBJECTIVE: To observe the pathological changes of brain-death donor lung, and to investigate the feasibility of its clinical transplantation. METHODS: Twenty-three cases of brain-death donor lung received pathological biopsy, and hematoxylin-eosin staining, silver staining and PAS staining were used to observe the pathological changes of lung, and the microstructure of lung tissues was observed under electron microscope. RESULTS AND CONCLUSION: Under the light microscope, bronchial and alveolar structure was complete, and edema, necrosis and defluxion could be observed in part of epithelial cells by hematoxylin-eosin staining, silver staining and PAS staining; alveolar interval was not widened obviously, but hyperemia was visible; small amount of bleeding and scattered lymphocytes could be seen around the vessels. Under the electron microscope, the slightly edema were observed in the brain-death alveolar cells, the cell nuclear chromatin condensed along the caryotheca, some nucleus appeared abnormity, mitochondria were engorgement, but no cell necrosis could be observed. Brain-death donor lung is suitable for clinical transplantation.