Guo X.,302 Hospital of PLA |
Yang M.,302 Hospital of PLA |
Gu H.,Xinjiang Medical University |
Zhao J.,302 Hospital of PLA |
Zou L.,301 Hospital of PLA
Cancer Epidemiology | Year: 2013
Background and aim: SOX6, a member of the D subfamily of sex determining region y-related transcription factors, plays critical roles in cell fate determination, differentiation and proliferation. It has been identified as a tumor suppressor or an oncogene in different human cancers. However, the role of SOX6 in the development and progression of hepatocellular carcinoma (HCC) has not been explored. The aim of this study was to investigate the expression of SOX6 in HCC and determine its correlation with tumor progression and prognosis. Methods: 130 HCC patients who had undergone curative liver resection were selected and immunohistochemistry, Western blotting, and quantitative real time polymerase chain reaction (Q-PCR) were performed to analyze SOX6 expression in the respective tumors. Results: Q-PCR, immunohistochemistry and Western blotting consistently confirmed the decreased expression of SOX6 at both mRNA and protein levels in HCC tissues compared with their adjacent nonneoplastic tissues (P< 0.01). Additionally, the expression of SOX6, determined by immunohistochemistry, was negatively correlated with the tumor stage (P= 0.003) and serum AFP (P= 0.02). Moreover, HCC patients with lower SOX6 expression had worse 5-year disease-free survival and 5-year overall survival than those with high SOX6 expression (P= 0.006 and 0.001, respectively). Furthermore, the Cox proportional hazards model showed that the decreased expression of SOX6 was an independent poor prognostic factor for both 5-year disease-free survival (hazards ratio [HR] = 2.398, 95% confidence interval [CI] = 1.601-5.993, P= 0.01) and 5-year overall survival (HR = 3.569, CI = 1.381-7.290, P= 0.008) in HCC. Conclusion: These findings provide evidence for the first time that SOX6 expression was decreased in HCC, which correlated with poor prognosis, suggesting that SOX6 may be a novel and potential prognostic marker for HCC. © 2013 Elsevier Ltd.
Gu H.,Xinjiang Medical University |
Guo X.,302 Hospital of PLA |
Zou L.,301 Hospital of PLA |
Zhu H.,301 Hospital of PLA |
Zhang J.,Xinjiang Medical University
Molecular and Cellular Biochemistry | Year: 2013
MicroRNA-372 (miR-372) has been demonstrated to play a crucial role in cellular proliferation and apoptosis of cancer cells. However, its effects in hepatocellular carcinoma (HCC) have not been explored. The aim of this study was to investigate the clinical significance of miR-372 in human HCC. Quantitative RT-PCR was performed to detect miR-372 expression in HCC clinical samples and cell lines. Then, Kaplan-Meier and Cox proportional regression analyses were performed to determine the association of miR-372 expression with survival of HCC patients. Moreover, the effects of miR-372 on tumorigenicity of HCC cell lines were evaluated by in vitro assays. miR-372 expression in HCC tissues was significantly higher than in the corresponding normal adjacent liver tissues (P < 0.001). There was a correlation between miR-372 upregulation and advanced TNM stage of HCC patients (P = 0.02). In addition, HCC patients with higher miR-372 expression had significantly poorer recurrence-free survival (P = 0.006) and overall survival (P = 0.001). Multivariate analysis revealed that high miR-372 expression was an independent predictor of poor prognosis (for recurrence-free survival: Hazard Ratio [HR] 6.826, P = 0.01; for overall survival: HR 9.533, P = 0.008). Moreover, in vitro assays demonstrated that the ectopic expression of miR-372 may significantly promote the cellular proliferation, invasion, and migration of HCC cell lines. Our findings showed that miR-372 may serve as a potent prognostic marker for tumor recurrence and survival of HCC patients. Furthermore, miR-372 has been identified as a promoter for tumorigenicity of HCC cells, suggesting that it might be a prospective therapeutic target for HCC. © 2013 Springer Science+Business Media New York.
Zhang Y.,Chinese Institute of Materia Medica |
Guo X.,302 Hospital of PLA |
Xiong L.,Beijing Institute of Radiation Medicine |
Kong X.,Chinese Institute of Materia Medica |
And 6 more authors.
FEBS Letters | Year: 2012
We previously showed that high expression levels of SOX9 correlate with hepatocellular carcinoma (HCC) progression. However, the exact role that SOX9 plays in HCC remains unclear. In this study, we firstly confirmed that miRNA-101 directly targets SOX9 in HCC. Ectopic expression of miR-101 significantly inhibited HCC cell proliferation and tumorigenicity by targeting SOX9. Moreover, the down-regulation of miR-101 in clinical HCC tissues correlates with tumor aggressiveness and poor prognosis. Therefore, miR-101 may suppress HCC tumor progression by down-regulating SOX9. MiR-101 may be a potential prognostic marker and therapeutic target for HCC. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Gao B.,301 Hospital of PLA |
Dong J.,301 Hospital of PLA |
Lu W.,301 Hospital of PLA |
Jiang H.,301 Hospital of PLA
Chinese Journal of Clinical Oncology | Year: 2010
Hepatocellular carcinoma is one of the most commonly occurring malignant tumors. The incidence of hepatocellular carcinoma is related with many factors, of which infection of hepatitis-B virus (HBV) is a key factor. This infection has already become a major worldwide problem, but the process leading to liver cancer remains unclear. The long-term carrying HBV has a significant relationship with the occurrence of hepatocellular carcinoma. HBV greatly promotes the development of hepatocellular carcinoma resulting from chronic hepatitis, but its specific mechanism of carcinogenesis is still controversial. Hypermethylation of the tumor suppressor gene was found to play an important role in the incidence, development and metastasis of the HBV- associated hepatocellular carcinoma. The hypermethylation of the tumor suppressor gene has an obvious specificity: it is closely related to the hepatitis B viral infection, and is maintained by DNA methyl-transferase 1. This phenomenon supplies new methods for diagnosis and treatment of the HBV-associated hepatocellular carcinoma. For early diagnosis, risk assessment and preventive treatment of the carcinoma, further discovery and understanding of the molecular mechanisms of HBV leading to the hepatocellular carcinoma is extremely necessary. Methylation of tumor suppressor gene can be used for early diagnosis of HBV-associated hepatocellular carcinoma. Hypermethylation of tumor suppressor gene silencing, by drugs which re-express the anti-oncogene, may become a research direction in the future.
Li G.,301 Hospital of PLA |
Qian Y.,301 Hospital of PLA |
Bai H.,301 Hospital of PLA |
Song Z.,301 Hospital of PLA |
And 4 more authors.
Archives of Surgery | Year: 2012
Objectives: To study the clinical anatomy of the transversalis fascia (TF) and to explore the intertransversalis fascia approach in urologic laparoscopic operations (ULOs). Design: Prospective study. Setting: Two academic hospitals. Other Participants: Data from 1217 urologic laparoscopic or open operations and 10 laparoscopic hernia repairs were analyzed between January 1, 2009, and April 30, 2011. Findings from 3 fresh autopsies were also included. Main OutcomeMeasures: The anatomy of the TF was studied and the intertransversalis fascia approach was explored in ULOs; furthermore, they were proved in the open operations and fresh autopsies. Photographs were taken from the intertransversalis fascia approach in ULOs, micrographs were obtained to examine the microscopic structure of the TF, and the color atlas of TF anatomy (cross and sagittal sections) was drawn. Results: The TF is a general plane of connective tissue lying between the inner surface of the transversus abdominis and the extraperitoneal fat. It can be divided into 2 layers (superficial and deep), with an amorphous fibroareolar space between them. The intertransversalis fascia approach in ULOs is the approach between the 2 layers of the TF. Conclusions: The intertransversalis fascia approach is described for the first time, to our knowledge. Surgeons can obtain a clean, clear, and bloodless operating space in ULOs using the intertransversalis fascia approach. ©2012 American Medical Association. All rights reserved.
Yao Y.,Second Affiliated Hospital |
Wang G.,Second Affiliated Hospital |
Wang Z.,Second Affiliated Hospital |
Wang C.,Second Affiliated Hospital |
And 2 more authors.
Journal of Oral and Maxillofacial Surgery | Year: 2011
Purpose: The previous decade has witnessed increasing emphasis on the technique of trans-sutural distraction osteogenesis (TSDO), a new and challenging procedure to reconstruct deficiencies in craniomaxillofacial bone. The purpose of this study was to determine if locally administered recombinant human bone morphogenetic protein-2 (BMP-2)/Poly(lactic-co-glycolic acid)/Fibrin sealant and recombinant human osteoprotegerin recombinant OPG fusion protein improves osteoblastogenesis and new bone formation by TSDO. Materials and Methods: Thirty-two dogs were divided into 4 groups: control, BMP-2, OPG, or BMP-2 plus OPG. Two dogs from each group were sacrificed at 1, 2, 4, and 6 weeks after initiating the DO protocol. Immunohistochemical, histomorphometric, and electron microscopic assessments were performed to investigate the effects of BMP-2 or OPG induced by TSDO. Results: The animals demonstrated significant overgrowth of the maxilla (control, 19.5 ± 2.61 mm; BMP-2, 19.9 ± 1.47 mm; OPG, 18.3 ± 1.2 mm; BMP-2 + OPG, 20.5 ± 2.65 mm). Histologically, the palatine suture widened dramatically within 2 weeks after distraction. Osteoblast number, trabecular thickness, content of alkaline phosphatase, and integrated optical density of BMP-2 increased obviously in the BMP-2 + OPG group (P <.05). Conclusions: TSDO in growing dogs is a safe, well-tolerated technology. The study found that OPG alone did not improve bone regeneration, but that it acted synergistically with BMP-2 to increase recruitment of mesenchymal stem cells and led to a significant enhancement of bone formation and healing. The temporal pattern of BMP-2 expression is consistent with a role in the regulation of mechanical and biological interventions designed to promote bone regeneration. © 2011 American Association of Oral and Maxillofacial Surgeons.