273 Hospital of Chinese PLA
273 Hospital of Chinese PLA
Li J.,PLA Fourth Military Medical University |
Liu G.,273 Hospital of Chinese PLA |
Zhang F.,PLA Fourth Military Medical University |
Zhang Z.,PLA Fourth Military Medical University |
And 2 more authors.
Molecular Medicine Reports | Year: 2017
Hepatic glycoprotein (gp78), a membrane-anchored E3 ubiquitin ligase, has been reported to be involved in regulating lipid and energy metabolism in animals, and cell death-inducing DFFA-like effectorc (cidec) has emerged as an important regulator of metabolism, which has been implicated in the process of fat differentiation. Nonalcoholic fatty liver disease is a metabolic disorder associated with hepatic steatosis. In the present study, to investigate the role of gp78 and cidec in hepatic steatosis, an in vitro cell culture model of hepatic steatosis was established, using the AML12 mouse hepatocyte cell line to assess the protein expression of gp78. The results of Oil Red O staining, phase contrast microscopy and triglyceride content detection experiments indicated that the overexpression of gp78 induced lipid accumulation, whereas gp78-knockdown led to a reduction in lipid accumulation in the AML12 cells. The increased expression of gp78 was associated with steatosis. The expression of cidec was consistent with gp78, and the colocalization of gp78 and cidec was observed on the surface of lipid droplets using im munofluorescence a na lysis. Furthermore, an interaction between gp78 and cidec was detected using coimmunoprecipitation analysis, and this interaction promoted lipid accumulation. Based on these data, it was hypothesized that gp78 is a regulator of hepatic steatosis, and that it may be a putative molecular mediator in metabolic diseases.
Tang G.-L.,273 Hospital of Chinese PLA |
Tao C.,273 Hospital of Chinese PLA |
Chen S.-B.,273 Hospital of Chinese PLA |
Han L.-F.,273 Hospital of Chinese PLA |
Huang X.-Z.,273 Hospital of Chinese PLA
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
OBJECTIVE: To explore the anti-shear force, stability and plasticity of reconstruction plate implantation, and to investigate its role in treating acetabular fractures complicated by free bone graft in the joint. METHODS: A computer-based online search of Science Direct and Ei databases (1960-01/2009-10) was performed for related English articles with the keywords of "acetabular fractures, modified ilioinguinal approach, reconstruction plate". In addition, CNKI and CBM database (1994-01/2009-10) were searched for related Chinese articles with the same key words in Chinese. Moreover, related works were manually searched. Studies regarding reconstruction plate implantation in treating acetabular fractures were included, including basic and clinical experiments. RESULTS: Acetabular top is weight loading region of human body, and the fracture should be reducted by the operation. Prior to reconstruction plate for acetabular fracture fixation, bone hook, and Kirschner wire can be used for temporary fixation, and the plate bending angle should be adjusted to accordant with lateral posterior wall or medial anterior wall of the acetabular top, followed by screw threading for fracture fixation. In addition, the screw entrance angle is very important. If the fixation of one plate is not satisfactory, one or two cancellous bone screw or Kirschner wire can be used to enhance the fixation. But the Kirschner wire tail should be bended to prevent dislocation into the pelvic cavity. Moreover, additional plate can be used for further fixation. Reconstruction plate fixation has advantages of anti-shear force, high stability and plasticity, which fully adapt the special appearance of acetabulum. Moreover, the incidence of postoperative plate loosening and breakage is low, and the satisfaction is high. Free bone blocks can be temporarily fixed by Kirschner wire, followed by reconstruction plate fixation. CONCLUSION: Reconstruction plate fixation has good clinical effect and few complications in treating acetabular fractures complicated by free bone blocks in the joint.
Du J.-J.,PLA Fourth Military Medical University |
Meng H.,PLA Fourth Military Medical University |
Cao Y.-J.,PLA Fourth Military Medical University |
Li F.-Q.,273 Hospital of Chinese PLA |
Luo Z.-J.,PLA Fourth Military Medical University
Journal of Spinal Disorders and Techniques | Year: 2012
Study Design: Case report. Objective: To report the clinical features, radiographic findings, treatments, and results of 2 children with cervical intervertebral disc calcification combined with ossification of the posterior longitudinal ligament (OPLL). Summary of Background Data: The calcification of the intervertebral disc, which is more frequent in males with predominant localization to cervical spine, was first reported by Baron in 1924. OPLL of the cervical spine, which is found approximately in the 5th to 7th decade of life, is a disease-causing spinal canal stenosis and spinal cord compression. The etiologies of these 2 diseases still remain unclear. Methods: An 8-year-old girl presented with progressive neck pain and complained of weakness and numbness of the upper left extremity, and a 6-year-old boy presented with complains of neck pain. X-ray, computed tomography, and magnetic resonance imaging findings of 2 patients confirmed the presence of cervical intervertebral disc calcification combined with OPLL. Results: Two children were treated using conservative treatment. The girl was observed up for 2 years and the boy was observed up for 18 months, respectively. Computed tomography and magnetic resonance imaging revealed that cervical intervertebral disc calcification and OPLL at the C6/7 (case 1) and C3/4 (case 2) level have disappeared completely, only a small calcification at the C2/3 intervertebral disc remained in the second case and both of them were asymptomatic. Conclusions: Cervical intervertebral disc calcification combined with OPLL was rarely observed in children. Conservative management was carried out and the patients had a full recovery. Our experience suggests that the conservative treatment is an acceptable method. © 2012 by Lippincott Williams & Wilkins.