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Ding W.-J.,Shanghai University | Ding W.-J.,264 Hospital of Chinese PLA | Tang Y.,Shanghai University | Song Y.-L.,Shanghai University | And 5 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

Background: Muscle-derived stem cells (MDSCs), a newly discovered adult stem cell possessing utility potential in tissue engineering and regenerative medicine, have been isolated from skeletal muscle tissue. The MDSCs isolating method varies a lot. Objective: To isolate and culture MDSCs and its clone as well as sub-clone through the use of modified preplate technique combined with limited dilution technique. Methods: The muscle tissue was obtained from new born mice under microscopy and then digested and filtered, from which MDSCs were isolated by modified preplate technique with first round plating period of 1 hour. The muscle derived cells were counted and MDSCs were marked with immunohistochemistry method. The MDSC clone and its subclone were obtained with limited dilution technique. Results and Conclusion: During the isolation procedure with preplate technique, muscle derived cells made up a progressively higher ratio in the cell culture and the procedure with first round plating period of 1 hour provided plenty cells for MDSCs isolation. MDSCs presented with adherent growth 72 hours after the sixth suspension, grew into cell population of 300-500 cells in 10 days about, and proliferated with its small round and spindle morphology persisted. MDSCs clone and sub-clone were obtained through limited dilution technique and found desmin positively expressed and Sca-1 positively expressed at ratio of (92.3±4.1)%. The MDSCs and its clone from mice may provide proper cell resource for tissue engineering and regenerative medicine research.

Ding W.-J.,264 Hospital of Chinese PLA | Ding W.-J.,Shanghai University | Su Z.-D.,Shanghai University | Jiang H.,264 Hospital of Chinese PLA | Jiang H.,Shanghai University
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Muscle satellite cells are a main cell source for the repair of muscle tissue injury, and have been paid extensive attention in the study of muscle tissue regeneration. Objective: To summarize the biological properties of muscle-derived stem cells and its application in the field of tissue engineering and regenerative medicine, and to explore the clinical application value and perspective of muscle-derived stem cells. Methods: We retrieved Vip database and PubMed database for articles concerning the application of muscle-derived stem cells published from January 2000 to April 2011. In titles and abstracts, the key words were "muscle-derived stem cells, muscle satellite cells, tissue engineering, regenerative medicine, adult stem cells, MDSCs, MSCs, ASCs". Articles addressing the application of muscle-derived stem cells were included, and those in the same field published recently or in authorized journals were included. 189 literatures were primarily collected, and a total of 25 literatures were included according to the inclusion criteria. Results and Conclusion: Animal experiments and success clinical experiments suggested that in combination of genetic engineering and tissue engineering, tissue engineered tissues and cytological therapy based on muscle-derived stem cells can be used in various skeletal muscle diseases, myocardial diseases, urinary system and nervous system by safe measures. This provides a good healing perspective for clinical physicians and patients, but the study of muscle-derived stem cells still faced many problems.

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