Zhangjiakou, China
Zhangjiakou, China

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Tao Y.,Shanghai University | Zhang J.,Shanghai University | Kong E.,Shanghai University | Pei S.,251 Hospital of PLA | And 4 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2016

The elevated pain threshold is a special change in obstructive jaundice (OJ). It does not attract enough attention, which leads to over-use of opioid drugs and causes many peri-operative complications. In the present study, β-endorphin in the plasma of OJ patients was measured by ELISA. Leukocytes were isolated from peripheral blood of the OJ and non-jaundice (Non-J) patients. The mRNA of β-endorphin precursor proopiomelanocortin (POMC) and μ-opioid receptor (MOR) in the leukocytes of OJ patients were measured by Real-time PCR. After treated with bilirubin, the leukocytes from Non-J patients were used to measure the expression of μ-opioid receptor and β-endorphin. Opioid-containing leukocytes were also measured by Flow Cytometry after bilirubin incubation. After the obstructive jaundice model of rats was established by bile duct ligation (BDL), the rats were injected with ICAM-1 antibody. The pain threshold of rats was detected and β-endorphin in the plasma was measured by ELISA method. The results showed that β-endorphin was higher in the OJ patients than that in the Non-J patients. The mRNA of POMC and MOR in the leukocytes were significantly higher in the OJ patients than that in the Non-J patients. After leukocytes from Non-J patients were treated with bilirubin, β-endorphin in the supernatant was up-regulated. The POMC mRNA of leukocytes was also increased. Meanwhile, the number of opioid-containing leukocytes was significantly raised after bilirubin incubation. In the rat model of OJ, elevated pain threshold was reversed after treated with ICAM-1 antibody (0.5 mg/kg, 1 mg/kg). Meanwhile, the β-endorphin in the plasma was down-regulated by ICAM-1 antibody. The results demonstrated that the up-regulation of β-endorphin is probably related to the elevated pain threshold in OJ patients. The results further suggested that the adhesion of leukocytes was involved in the up-regulation of β-endorphin and MOR as well as elevated pain threshold among OJ patients. The changes in opioid-containing leukocytes are probably an alternative explanation to this special pain change in OJ patients.


Yan Y.-H.,251 Hospital of PLA | Shang P.-Z.,251 Hospital of PLA | Lu Q.-J.,251 Hospital of PLA | Wu X.,Peking University
Food and Chemical Toxicology | Year: 2012

Triptolide(TPT) isolated from one of the Chinese herbs, Tripterygium wilfordii Hook. F. (TWHF), are known to have a variety of immunomodulatory activities. This study was performed to investigate the effect of TPT on the differentiation of splenic DCs and its influence on T cell-mediated immunity regarding to DC subsets CD11c lowI-a/e lowCD45RB +(CD11c low DCs) and CD11c highI-a/e highCD45RB - (CD11c high DCs) in male C57BL/6 mice spleens in vitro. The percentage of CD11c low DCs was significantly increased after treatment with TPT compared to their counterparts (CD11c high DCs). It was found that unlike the gradually decreasing interleukin (IL)-12 secretion of CD11c high DCs induced by TPT, CD11c low DCs showed a obvious dose-dependent response between the increasing of IL-10 production and TPT stimulation. After treatment with anti-IL-12R or anti-IL-10 monoclonal antibody in CD4 + T cells+CD11c high DCs or CD11c low DCs mixed lymphocyte reaction, the induction of these DCs on T cells was inhibited dramatically. These data demonstrated that TPT might induce the differentiation of splenic DCs to CD11c low DCs followed by shifting of Th1 to Th2 with enhancement of T lymphocyte immune function in vitro. © 2012 .


Huang Y.,251 Hospital of PLA
International Journal of Clinical and Experimental Pathology | Year: 2014

Basal cell adenoma (BCA) is a specific entity that lacks the myxochondroid stromal component of a pleomorphic adenoma. There are six histopathological types of BCA: solid, tubular, trabecular, membranous, cribriform, and myoepithelial-derived stroma rich. Myoepithelial-derived stroma rich variant is so rare, especially with cellular atypia. Herin we describe a rare case of BCA arising in the parotid on a 25-year-old man. A well-demarcated nodule arising in the parotid that was composed of basaloid cells, forming small duct-like or tubular structures containing basement membrane-like material, as well as highly cellular elongated cells with hyperchromatic, enlarged, pleomorphic, and bizarre nuclei. Immunohistochemically, S100 protein and p63 highlighted the basal aspect of the peripheral epithelial cells and peripheral spindle and bizarre cells, while CK7 expressed on the luminal cells. We made a diagnosis of "basal cell adenoma, myoepithelial-derived stroma rich variant, with bizarre myoepithelial proliferation". The differential diagnosis includes cellular pleomorphic adenoma, basal cell adenocarcinoma, and carcinoma ex pleomorphic adenoma. After follow-up for 3 years, there was no evidence of recurrence. Further pathological characteristics of this disease are discussed.


Yin Y.-H.,Hebei North University | Wang H.-P.,Hebei North University | Yin S.-J.,251 Hospital of PLA | Liu H.-Q.,251 Hospital of PLA
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2013

Objective: To investigate the microanatomic and surgical exposure of supraorbital extradural approach to cavernous sinus under endoscope, so as to provide the microanatomic basis for clinical neurosurgery. Methods: Twenty fresh cadaver heads were studied using 4 mm 0° and 30° endoscopes to develop a surgical approach and identify surgical landmarks. Results: The endoscopic supraobital extradural approach could be divided into 3 stages: entry into the extradural anterior cranial fossa, exposure of the middle cranial fossa and periorbita, and exposure of the superior and lateral cavernous sinus. This approach could expose superb views of the cavernous sinus microanatomy especially through the Dolence triangle, Parkinson triangle and Mullan triangle. Conclusion: Endoscopic supraobital extradural approach offers excellent microanatomy of the cavernous sinus with minimal invasiveness, which is worthy of introduction.


Wang H.-S.,251 Hospital of PLA | Wang H.,251 Hospital of PLA | Xu X.-W.,251 Hospital of PLA | Yang Z.-W.,251 Hospital of PLA | And 3 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2013

Obbjective To evaluate the diagnostic value of 64-slice spiral CT angiography (CTA) in multiple intracranial aneurysms (MIA). Methods Twenty-five patients (9 males and 16 females, aged from 17 to 68 years) with suspected MIA underwent 64-slice spiral CTA with slice thi ckness of 0.625mm. The data of all patients were reconstructed with multiplanar reconstruction (MPR), 3-dimensional shade surface display (3-SSD), volume rendering (VR) and maximum intensity projection (MIP). The clinical diagnostic value was evaluated with the results of surgery or interventional embolization therapy. Results A total of 61 aneurysms were detected by 64-slice spiral CTA in 25 patients, among them 17 patients were with 2 aneurysms, 6 patients with 3 aneurysms, 1 patient with 4 aneurysms and 1 patient with 5 aneurysms. The 64-slice spiral CTA could not only clearly demonstrate MIA, but also the size, neck, axis point and parent artery of each aneurysm, and the spatial relationship between aneurysm and the adjacent vessels and bone structures. Twenty-one patients underwent microsurgery and four underwent embolization, the location, size and shape of aneurysm and its spatial relationship with adjacent structures were in accordance with the findings of 64-slice spiral CTA. Conclusion The 64-slice spiral CTA could be used as the first choice in the diagnosis of MIA for its high accuracy, and may be an important adjunct to digital subtraction angiography (DSA) in MIA treatment.


Huang Y.,251 Hospital of PLA | Li H.,251 Hospital of PLA | Xiong Z.,251 Hospital of PLA | Chen R.,Chongqing Cancer Institute
International Journal of Clinical and Experimental Pathology | Year: 2014

The great majority of malignant peripheral nerve sheath tumors (MPNSTs) exhibit Schwann differentiation. Few MPNSTs with perineurial differentiation are also named malignant perineuriomas. Benign perineurioms were classified as intraneural, extraneural (soft tissue), sclerosing, and reticular variant. Histopathological features of intraneural perineurioma are individual nerve axons surrounded by whorls of spindle-shaped cells arranged in an onion bulb-like pattern. However, intraneural malignant perineurioma is uncommon, its characteristic histological features were not clearly described yet. Positive for epithelial membrane antigen (EMA), glut-1 and claudin-1, is characteristic of malignant perineurioma. Herein, we report an intraneural malignant perineurioma in median nerve of a 13-year-old girl. The clinicopathological features were summarized and the related literatures were reviewed.


Huang Y.,251 Hospital of PLA | Yang M.,251 Hospital of PLA | Ding J.,251 Hospital of PLA
International Journal of Clinical and Experimental Pathology | Year: 2014

Basal cell adenoma (BCA) is a specific entity that lacks the myxochondroid stromal component of pleomorphic adenoma. Membranous basal cell adenoma is a rare variant of BCA, which is characteristic by abundant eosinophilic extracellular hyaline material deposited either inside or at the periphery of the epithelial islands. Herin we describe the first case of membranous BCA arising in the upper eyelid in a 38-year-old woman. A well-demarcated nodule arising in the eyelid was composed of isomorphic basaloid cells organized with a prominent basal cell layer and distinct basement membrane-like material. Immunohistochemically, S100 protein and p63 highlighted the basal aspect of the peripheral epithelial cells, while CK7 expressed on the luminal cells. A diagnosis of membranous basal cell adenoma of the eyelid was made. At follow-up for 2 years and 3 months later, there was no evidence of recurrence. Further pathological characteristics of this disease are discussed.


Wang Y.,Tangshan Gongren Hospital | Han X.-C.,Hebei North University | Zheng L.-Q.,251 Hospital of PLA | Miao W.-L.,Hebei North University
International Journal of Clinical and Experimental Pathology | Year: 2014

Xanthogranulomatous cystitis is a rare, benign chronic inflammatory disorder with unclear etiology. Since its similar features to bladder neoplasm, it is extremely easy to be mistaken for malignant tumor. We herein reported a case of xanthogranulomatous cystitis in a 54-year-old male who presented with low abdominal pain and painless, total macrohematuria. Microscopy showed proliferation of large foam histiocytes which expressed CD68 and vimentin. Ki-67 showed a lower proliferation index (< 10%). Histopathology and immunohistochemical findings confirmed the diagnosis. This case highlighted the significance of recognizing this unusual lesion and differentiating it from its histological mimics by immunohistochemical staining.


Huang Y.,251 Hospital of PLA | Han X.-C.,Hebei North University | Lv G.-S.,251 Hospital of PLA
International Journal of Clinical and Experimental Pathology | Year: 2014

Hemangioblastoma is a rare neoplasm occurred in soft tissue. Herein, we present an intriguing case of sporadic hemangioblastoma in the retroperitoneum occurring in a 59-year-old male. The tumor was characterized by an alternation of cellular and paucicellular areas surrounded by a thick fibrous capsule. The stromal cells showed a pale cytoplasm exhibiting occasional lipid droplets and hyperchromatic, irregularly nuclei. CD56, NSE, a-inhibin and S100 were positive in tumor cells. This is the third reported case of a hemangioblastoma in the retroperitoneum. Based on clinical examination, computed tomography imaging, surgical operation, and histopathologic studies, a definitive diagnosis was made. Further characteristic images and pathology of this disease are discussed.


PubMed | 251 Hospital of PLA
Type: Journal Article | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2012

Triptolide(TPT) isolated from one of the Chinese herbs, Tripterygium wilfordii Hook. F. (TWHF), are known to have a variety of immunomodulatory activities. This study was performed to investigate the effect of TPT on the differentiation of splenic DCs and its influence on T cell-mediated immunity regarding to DC subsets CD11c(low)I-a/e(low)CD45RB(+)(CD11c(low) DCs) and CD11c(high)I-a/e(high)CD45RB(-) (CD11c(high) DCs) in male C57BL/6 mice spleens in vitro. The percentage of CD11c(low) DCs was significantly increased after treatment with TPT compared to their counterparts (CD11c(high) DCs). It was found that unlike the gradually decreasing interleukin (IL)-12 secretion of CD11c(high) DCs induced by TPT, CD11c(low) DCs showed a obvious dose-dependent response between the increasing of IL-10 production and TPT stimulation. After treatment with anti-IL-12R or anti-IL-10 monoclonal antibody in CD4(+) T cells+CD11c(high) DCs or CD11c(low) DCs mixed lymphocyte reaction, the induction of these DCs on T cells was inhibited dramatically. These data demonstrated that TPT might induce the differentiation of splenic DCs to CD11c(low) DCs followed by shifting of Th1 to Th2 with enhancement of T lymphocyte immune function in vitro.

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