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Takamatsu-shi, Japan

Okuda Y.,217 20 Hayashi cho | Takahashi S.,217 20 Hayashi cho | Kawabata K.,RIKEN | Aonuma H.,Research Institute for Electronic Science | And 2 more authors.
Proceedings - 2013 1st International Symposium on Computing and Networking, CANDAR 2013 | Year: 2013

Recently, research on the habit of creatures and their characteristics are widely based on image processing. For example, the cricket Gryllus bimaculatus which consist of micro brain modifies its behavior by the situation and experience. In this paper, we constructed a technique of image observation to detect a cricket based on an advanced statistical feature, and automatically generated action trajectory. Therefore, we are able to apply the simultaneous observation of crickets. Through ex-perimentation, we proved the effectiveness of our proposed method. © 2013 IEEE. Source


Nagase N.,217 20 Hayashi cho | Terao K.,217 20 Hayashi cho | Miyanishi N.,1 1 Izumino | Tamai K.,Rexxam Co. | And 5 more authors.
Analyst | Year: 2012

We propose a novel Kretschmann-type surface plasmon resonance (SPR) sensor chip having a surface covered with electrodeposited gold nanostructures to enhance the sensitivity of SPR biosensing. The nanostructure is three-dimensional and has a larger surface area than a conventional flat surface chip, which increases the amount of protein binding and also induces a large change in the effective dielectric constant of the sensing area. The gold nanostructures were formed by electrodeposition under galvanostatic conditions, so their size could be controlled by manipulating the deposition time and current. The sensing characteristics, including the concentration dependence and noise level, indicated that the performance of the resulting chip (called a Au-black chip) was equivalent to that of a conventional sensor chip. We also determined the optimal electrodeposition conditions to obtain a sharp SPR curve for protein detection assay; the optimal thickness of the gold layer was 50-60 nm. Enhanced protein sensing was demonstrated by using a binding assay of anti-BSA antibody and BSA molecules. The protein binding signal was several times higher than that of the conventional assay. The insights into electrodeposition for SPR sensing presented here will enable improved sensitivity for detecting low-concentration and small proteins. © 2012 The Royal Society of Chemistry. Source


Tanaka R.,217 20 Hayashi cho | Okuda Y.,217 20 Hayashi cho | Takahashi S.,217 20 Hayashi cho | Oda S.,Tokyo University of Science
Proceedings - 2013 1st International Symposium on Computing and Networking, CANDAR 2013 | Year: 2013

Recently, image processing is widely used in research on the habits and characteristics creatures. For example, the medaka (Oryzias laptipes) which breeds quickly is popular used in various experiments. In particular, observing the behavior of the medaka is important to understanding the ecology of their characteristics. In this paper, we proposed the tracking method based on image processing for a medaka observation of its behavior. Therefore, we describe our proposed tracking method which combine the estimated position and the orientation code matching based on rotation invariant feature. Through experimentation, we examined the effectiveness of our proposed method. © 2013 IEEE. Source

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