Tan G.,Dalian Medical University |
Xu L.,Jilin University |
Fang Y.,Jilin University |
Tao G.,210th Hospital of PLA |
And 2 more authors.
Chinese Journal of Clinical Oncology | Year: 2010
Objective: To investigate the anti-tumor effect of cytotoxic T lymphocytes (CTLs) activated by dendritic cells (DCs) pulsed with K-ras antigen. Methods: DCs were generated from PBMC in the presence of GM-CSF and IL-4 in vitro. DCs were sensitized with pancreatic cancer cell line expressing K-ras mutant, K-ras (12-Val) mutant peptide, K-ras (12-Val) mutant peptide with the surface of cationic nanoparticle. The mature DCs were cocultured with autologous T cells to obtain antigen specific CTL. The anti-tumor activity of CTL in vivo was evaluated in BALB/c nude mice bearing Patu8988 (K-ras+) and SW1990 (K-ras-) pancreatic cancer. Results: CTLs cocultured with DCs with the whole tumor antigen significantly inhibited the growth of transplanted pancreatic cancer (Patu8988 and SW1990) in nude mice. In vivo experiments showed that DCs with K-ras (12-Val) mutant peptide and K-ras mutant peptide with the surface of cationic nanoparticle induced killing activity of specific CTL aganist pancreatic cancer cell line Patu8988 (K-ras+) (P<0.05). Conclusion: CTL induced with DCs pulsed with K-ras antigen can significantly improve the survival of nude mice with pancreatic cancer, through inhibiting tumor growth.
Yang B.,210th Hospital of PLA |
Wu G.,General Hospital of Shenyang Military Command |
Wang X.,General Hospital of Shenyang Military Command |
Zhang X.,General Hospital of Shenyang Military Command
Tumor Biology | Year: 2013
This study aims to investigate the prognostic significance of reclassification of stage IV gastric cancers in conjunction with Borrmann type. A total of 1,673 gastric cancer patients who received a gastrectomy between 1980 and 2003 were retrospectively evaluated. Of the patients, 244 (14.58 %), 344 (20.56 %), 589 (35.21 %), and 496 (29.65 %) had stage I, II, III, and IV cancers, respectively. After Cox regression analysis, Borrmann type was identified to be the independent prognostic factor in stage IV gastric cancer. The disease-specific postoperative survival of patients with Borrmann I, II, and III tumors was clearly distinguished by TNM classification system (P < 0.05), while it failed to classify Borrmann IV tumors (P = 0.147). Interestingly, the disease-specific postoperative survival of stage IV patients with Borrmann IV tumors (group 1) was significantly poor than the cases with stage IV but not Borrmann IV tumors (group 2), as well as the patients with Borrmann IV while not included in stage IV tumors (group 3) (P = 0.022 and P = 0.000, respectively). Meanwhile, the disease-specific postoperative survival was not observed as significantly different between group 2 and group 3 (P = 0.063); furthermore, group 2 + 3 had a better prognosis than group 1 (introduced stage IVa vs. stage IVb; P = 0.006). Reclassification of stage IV through combining the present TNM classification system with Borrmann type may more accurately predict the prognosis of patients. © 2013 International Society of Oncology and BioMarkers (ISOBM).
Sun C.,Xi'an Jiaotong University |
Wang L.,Xi'an Jiaotong University |
Wang Z.,PLA Fourth Military Medical University |
Geng L.,PLA Fourth Military Medical University |
And 3 more authors.
Journal of Mechanics in Medicine and Biology | Year: 2015
Custom-made knee prostheses have been widely used to reconstruct the function of the lower limb in bone tumor resections. A custom-made tumor knee prosthesis was retrieved on account of prosthesis loosening post-surgery. Misalignment between the anatomical axis of the femur and the axis of the femoral stem as well as the material loss at the posterior region of the tibial plateau were considered to be the primary causes of the failure. Based on this hypothesis, finite element analysis was performed to investigate the contact mechanics of the prosthesis while implanted in vivo. The maximum deformation at the femur was 0.59 and 1.17 mm when the misalignment angle was 3° and 6°, respectively. Besides, the maximum contact pressure at the tibial plateau was 44.88 MPa at an extremely high flexion of angle 135° during squatting or kneeling. Uneven stress distribution at the femur, which came from the misalignment, was the main cause of loosening, which was aggravated indirectly with the material loss at the posterior region of the tibial plateau. Optimized prosthesis design and appropriate selection, with accurate surgical positioning and targeted rehabilitation training programme are important considerations for prolonging life-span of prostheses in vivo. © 2015 World Scientific Publishing Company.
Yang X.-B.,Shenyang Pharmaceutical University |
Wang X.-B.,Shenyang Pharmaceutical University |
Wang X.-B.,210Th Hospital of PLA |
Pan W.-S.,Shenyang Pharmaceutical University |
And 5 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2011
In this study, dry powder formulations for inhalation of fanhuncaoin, a newly discovered antiinflammatorily active compound isolated from Chinese herb, were designed to optimize the composition and further explore the relationship between the composition, the physical properties and the aerosolization performance. Dry powders were prepared by spray-drying using leucine, chitosan, chitosan oligosaccharide and dipalmitoyl phosphatidylcholine (DPPC) as excipients. Following spray-drying, resultant powders were characterized using scanning electron microscopy, tapped density analysis, laser diffractometry, thermogravimetric analysis and differential scanning calorimetry. The aerosol behaviour of the powders was studied in a Twin Stage Impinger at an airflow rate of 60 l/min using a HandiHaler® inhaler device. Results revealed that the nature and the relative proportion of the excipients greatly influenced the physical characteristics of the powders and their aerodynamic behavior. Among the combinations tested, the composition ratio of fanhuncaoin/leucine/chitosan/ chitosan oligosaccharide/DPPC of 10/45/33.75/11.25/0.4 (w/w/w/w/w) prepared in a total solid mass of 1% (w/v) formulation was found to be particularly optimal and exhibited a tapped density of 0.44 g/cm 3, an aerodynamic diameter of 2.24 μm and an respirable fraction of 51.29%. In conclusion, optimization of the aerosolization properties of inhalation dry powders could be achieved by appropriately selecting the composition of the particles. © 2011 Pharmaceutical Society of Japan.
Shi X.,PLA Fourth Military Medical University |
Shi X.,210th Hospital of PLA |
Jin L.,PLA Fourth Military Medical University |
Dang E.,PLA Fourth Military Medical University |
And 4 more authors.
Journal of Investigative Dermatology | Year: 2011
Psoriasis, an immunological skin disease, is characterized by epidermal hyperproliferation, chronic inflammation, and an accumulation of infiltrating T cells. IL-17A is a key cytokine that has a critical role in the pathogenesis of psoriasis. Keratin 17 (K17) is strongly expressed in psoriatic lesions but not in normal skin. Thus, K17 expression is regarded as a hallmark of psoriasis. We previously reported that the K17/T cells/cytokine autoimmune loop was involved in psoriasis. However, the relationship between IL-17A and K17 has yet to be determined. In the present study, IL-17A-induced K17 expression was confirmed in cultured keratinocytes in both mRNA and protein levels. In addition, increased K17 expression was found in the epidermis of IL-17A-injected mouse skin. The regulatory mechanism of K17 expression was further investigated. We found that both the signal transducer and activator of transcription (STAT) 1 and STAT3 pathways were involved in the upregulation of K17 expression induced by IL-17A, and that such regulation could be partially suppressed by STAT1 or STAT3 small interfering RNA and inhibitor. Our data suggest that IL-17A can upregulate K17 expression in keratinocytes in a dose-dependent manner through STAT1-and STAT3-dependent mechanisms. The results indicate that IL-17A might be an important cytokine in the K17/T cells/cytokine autoimmune loop associated with psoriasis. © 2011 The Society for Investigative Dermatology.
Wang X.-B.,210th Hospital of PLA |
Wang X.-B.,Shenyang Pharmaceutical University |
Xi R.-G.,210th Hospital of PLA |
Yang X.-B.,210th Hospital of PLA |
And 4 more authors.
Chinese Journal of New Drugs | Year: 2010
The formulation factors, including physical characteristics of dry powder inhalations, excipients and dry powder inhalers, is very important in achieving adequate delivery of inhaled drug to lungs. Here dry powder inhalations are classified into following four types (carrier-free, drug-carrier, drug-additive and drug-carrier-additive) and analyzed.
Xiang Y.,210th Hospital of PLA
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010
To investigate the effect of post-remission therapy mainly with Compound Huangdai Tablet (CHDT) on long-term survival of patients with acute promyelocytic leukemia (APL). One hundred and twelve APL patients were treated after remission mainly with CHDT administered alternately with chemotherapeutic projects such as HACP, HAOP, HAEP and HAMP. The relapse rate and relapse-free survival (RFS) rate in them were estimated by bone marrow examination. The total relapse rate was 14.29% (16/112), and the median time of relapse was 12.5 (4-67) months. Patients were followed up for 1-72 months, the median follow-up time being 59 months. The actual RFS rate of 1-, 2-, 3-, 4-, 5- and 6-year was 92.86%, 89.29%, 88.39%, 87.50%, 86.61% and 85.71%, respectively, while the estimated RFS rate (%) of corresponding year was 92.45 +/- 2.57, 88.25 +/- 3.20, 87.09 +/- 3.36, 85.89 +/- 3.52, 84.44 +/- 3.75 and 82.78 +/- 4.03 respectively; the relapse rate in patients who received treatment after complete response for <10 courses group was 34.29%, while in those treated for > or = 10 courses was 5.19%; and the RFS rate in them was 65.71% and 94.81% respectively, the difference between groups was statistically significant (P<0.01). The post-remission therapy mainly with CHDT is an effective and feasible program for the treatment of APL.
PubMed | 210th Hospital of PLA
Type: Journal Article | Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine | Year: 2011
To investigate the effect of post-remission therapy mainly with Compound Huangdai Tablet (CHDT) on long-term survival of patients with acute promyelocytic leukemia (APL).One hundred and twelve APL patients were treated after remission mainly with CHDT administered alternately with chemotherapeutic projects such as HACP, HAOP, HAEP and HAMP. The relapse rate and relapse-free survival (RFS) rate in them were estimated by bone marrow examination.The total relapse rate was 14.29% (16/112), and the median time of relapse was 12.5 (4-67) months. Patients were followed up for 1-72 months, the median follow-up time being 59 months. The actual RFS rate of 1-, 2-, 3-, 4-, 5- and 6-year was 92.86%, 89.29%, 88.39%, 87.50%, 86.61% and 85.71%, respectively, while the estimated RFS rate (%) of corresponding year was 92.45 +/- 2.57, 88.25 +/- 3.20, 87.09 +/- 3.36, 85.89 +/- 3.52, 84.44 +/- 3.75 and 82.78 +/- 4.03 respectively; the relapse rate in patients who received treatment after complete response for <10 courses group was 34.29%, while in those treated for > or = 10 courses was 5.19%; and the RFS rate in them was 65.71% and 94.81% respectively, the difference between groups was statistically significant (P<0.01).The post-remission therapy mainly with CHDT is an effective and feasible program for the treatment of APL.