Changchun, China
Changchun, China

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Zhao W.-C.,208th Hospital of PLA | Hou Z.-H.,Hospital Office | Zhang Q.,208th Hospital of PLA | Zheng W.,208th Hospital of PLA
Asian Pacific Journal of Tropical Medicine | Year: 2014

Objective: To study the various processes involved in transcellular transport (TT) of huperzine A alone or in combination with ginkgolide B in Caco-2 and Madin-Darby canine renal (MDCK) cell monolayer. Methods: The transepithelial passage was assayed in the apical-to-basolateral (AP to BL) direction and opposite direction (BL to AP) in both cell lines. The determination of huperzine A and ginkgolide B were performed by high performance liquid chromatography (HPLC). The passage rates of huperzine A and ginkgolide B were calculated. Bi-directional TT (absorption and secretion) were taken in huperzine A and ginkgolide B in Caco-2 and MDCK cell monolayer. Results: TT absorption and secretion kinetics of huperzine A and ginkgolide B across two cells existed at the same time. The passage rates of huperzine A were increased significantly with adding different concentrations of ginkgolide B. Conclusions: The compound preparations of HA in combination with GB for dementia caused by cerebral ischemic have synergistic effects on the pharmacodynamics, and improve the bioavailability through BBB. © 2014 Hainan Medical College.


Wang J.,Xiamen University | Cui W.,Ningbo University | Ye J.,Xiamen University | Ji S.,University of South China | And 5 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2012

Although therapeutic cellular angiogenesis is effective for chronic ischemia, the optimal mode of cellular administration is still under exploration. This study aimed to develop a cellular delivery system to enhance the perfusion and angiogenesis in the ischemic hind limb. Collagen scaffold (CS) was prepared, and for morphology and toxicity analysis, bone marrow-derived mesenchymal stem cells (BMSCs) were isolated, expanded, filtrated, and seeded onto CS to construct BMSCs-CS. The ischemic hind limbs of rabbit models were implanted with autologous BMSCs-CS, CS, and autologous BMSCs; the untreated ischemic or normal animals were considered as the ischemic or normal control groups. Oxygen saturation parameters were regularly measured to determine the perfusion in the extremities. Histological examinations with hematoxylin and eosin immunostaining against von Willebrand factor and smooth muscle (SM) α-actin were performed for capillary and mature vessel evaluation. CS was a multiporous structure without cytotoxicity. At several intervals, the oxygen saturation ratio (OSR) in normal control was the highest. The OSRs in BMSCs-CS and CS were higher than that in BMSCs and ischemic control (p < 0.05); the OSR in BMSCs-CS group was higher than that in CS at 6 and 8 weeks (p < 0.05). The capillaries in BMSCs-CS and CS were higher than that in CS, BMSCs, and the ischemic or normal control (p < 0.05). The mature vessels in BMSCs-CS were higher than that in CS, BMSCs, and the ischemic or normal control (p < 0.05). The autologous cellular delivery system proved to be an effective approach for improving higher ischemic hind limb perfusion and angiogenesis as opposed to cellular therapy alone. Copyright © 2012 Wiley Periodicals, Inc.


Wang R.,Jilin University | Wang R.,Inner Mongolia University | Wang Y.,Inner Mongolia University | Zhang R.,Peoples Hospital of Inner Mongolia Autonomous Region | And 2 more authors.
Journal of Pediatric Surgery Case Reports | Year: 2014

Colonic perforation is an extremely rare complication following ventriculoperitoneal (VP) shunting. The common treatment is to remove the perforating catheter and replace it with a new one. Here we report a case of colonic perforation from VP shunting in a 2-year, 8-month-old boy who presented with the distal end of the shunt catheter protruding out of his anus. The distal catheter was removed via the anus and the perforation repaired transanally. The patient's postoperative course was uneventful. This case reminds us that we should not make another rush to perform a new shunt operation unless there are some manifestations of hydrocephalus. Potential mechanisms of migration and its management strategy are discussed. © 2014 The Authors.


Liu S.,Jilin University | Guo Q.,Jilin University | Han H.,208th Hospital of PLA | Cui P.,Jilin University | And 4 more authors.
International Urology and Nephrology | Year: 2016

Objective: To investigate the clinical and histopathological features of non-diabetic renal disease (NDRD) superimposed on diabetic nephropathy (DN) in northeastern Chinese patients with type 2 diabetes mellitus (T2D), and compare the changes with those of pure DN and isolated NDRD. Methods: Single-center retrospective analysis based on medical records of 273 patients (172 men, mean age: 51.1 ± 12.4 years) with T2D who underwent renal biopsy between February 2000 and October 2015. All patients were diagnosed as cases of pure DN, isolated NDRD or NDRD superimposed on DN. Results: Out of the 273 T2D patients, 68 (24.9 %) had DN, 175 (64.1 %) had NDRD, and 30 (11.0 %) had NDRD superimposed on DN. Idiopathic membranous nephropathy (IMN, 29.7 %) was the most common NDRD followed by IgA nephropathy (IgAN, 22.9 %), and hypertensive renal arteriolar sclerosis was the most common lesion in patients diagnosed as NDRD superimposed on DN. Patients with NDRD had a shorter duration of diabetes and lower frequencies of diabetic retinopathy (DR, 6.9 %) and renal failure (28.0 %), which is consistent with higher estimated glomerular filtration rates (eGFR) and lower systolic blood pressure (SBP). No significant between-group differences were observed with respect to proteinuria and hematuria. Conclusion: Renal biopsy is strongly recommended for T2D patients to distinguish DN, NDRD and NDRD superimposed on DN, especially in patients with no signs of DR. This approach may help in early diagnosis and treatment of NDRD and improve renal outcomes in northeastern Chinese T2D patients. © 2016, The Author(s).


Liu R.,PLA Fourth Military Medical University | Xu H.,University of California at Los Angeles | Wang G.,208th Hospital of PLA | Li J.,451 Hospital of PLA | And 4 more authors.
PLoS ONE | Year: 2013

Background: The pathogenesis of extraocular muscle (EOM) weakness in myasthenia gravis might involve a mechanism specific to the EOM. The aim of this study was to investigate characteristics of the EOM related to its susceptibility to myasthenia gravis. Methods: Female F344 rats and female Sprague-Dawley rats were assigned to experimental and control groups. The experimental group received injection with Ringer solution containing monoclonal antibody against the acetylcholine receptor (AChR), mAb35 (0.25 mg/kg), to induce experimental autoimmune myasthenia gravis, and the control group received injection with Ringer solution alone. Three muscles were analyzed: EOM, diaphragm, and tibialis anterior. Tissues were examined by light microscopy, fluorescence histochemistry, and transmission electron microscopy. Western blot analysis was used to assess marker expression and ELISA analysis was used to quantify creatine kinase levels. Microarray assay was conducted to detect differentially expressed genes. Results: In the experimental group, the EOM showed a simpler neuromuscular junction (NMJ) structure compared to the other muscles; the NMJ had fewer synaptic folds, showed a lesser amount of AChR, and the endplate was wider compared to the other muscles. Results of microarray assay showed differential expression of 54 genes in the EOM between the experimental and control groups. Conclusion: Various EOM characteristics appear to be related to the increased susceptibility of the EOM and the mechanism of EOM weakness in myasthenia gravis. © 2013 Liu et al.


PubMed | Jilin University and 208th Hospital of PLA
Type: Journal Article | Journal: International urology and nephrology | Year: 2016

To investigate the clinical and histopathological features of non-diabetic renal disease (NDRD) superimposed on diabetic nephropathy (DN) in northeastern Chinese patients with type 2 diabetes mellitus (T2D), and compare the changes with those of pure DN and isolated NDRD.Single-center retrospective analysis based on medical records of 273 patients (172 men, mean age: 51.112.4years) with T2D who underwent renal biopsy between February 2000 and October 2015. All patients were diagnosed as cases of pure DN, isolated NDRD or NDRD superimposed on DN.Out of the 273 T2D patients, 68 (24.9%) had DN, 175 (64.1%) had NDRD, and 30 (11.0%) had NDRD superimposed on DN. Idiopathic membranous nephropathy (IMN, 29.7%) was the most common NDRD followed by IgA nephropathy (IgAN, 22.9%), and hypertensive renal arteriolar sclerosis was the most common lesion in patients diagnosed as NDRD superimposed on DN. Patients with NDRD had a shorter duration of diabetes and lower frequencies of diabetic retinopathy (DR, 6.9%) and renal failure (28.0%), which is consistent with higher estimated glomerular filtration rates (eGFR) and lower systolic blood pressure (SBP). No significant between-group differences were observed with respect to proteinuria and hematuria.Renal biopsy is strongly recommended for T2D patients to distinguish DN, NDRD and NDRD superimposed on DN, especially in patients with no signs of DR. This approach may help in early diagnosis and treatment of NDRD and improve renal outcomes in northeastern Chinese T2D patients.


Yan L.,Jilin Institute of Architecture and Civil Engineering | Dong W.-Y.,208th Hospital of PLA | Bo W.,Jilin Institute of Architecture and Civil Engineering
Proceedings of the 2013 3rd International Conference on Intelligent System Design and Engineering Applications, ISDEA 2013 | Year: 2013

Bamboo has many good features, is an ideal material for the construction industry. The numerical simulation of multi-layer seismic frame structure of bamboo by hand shows that, for re-bamboo wood columns, typical of the two failure modes for the transverse deformation is too large can not continue to load and top of the fibers in the lateral pressure exploded; bamboo column can be restored in the unloaded more than 80% of deformation, has excellent elastic recovery; bamboo can withstand the load beam is actually controlled by the stiffness, not strength. © 2012 IEEE.


Objective: To evaluate the clinical significance of neutrophil gelatinase associated lipocalin (NGAL), also called human neutrophil lipocalin (HNL), in differential diagnosis of acute bacterial and viral infections. Methods: A double antibody sandwich ELISA method for NGAL/HNL was developed. A randomized single blind trial was performed in 250 patients with simple acute infectious diseases (including 150 cases of bacterial infection and 100 cases of viral infection) who visited the 208th Hospital of PLA in China from April 2010 to March 2011. The NGAL/HNL contents in sera of patients were determined by the developed method, based on which the clinical significance of NGAL/HNL in differential diagnosis of acute bacterial and viral infections was evaluated by comparing the determination results with the practical diagnosis in clinic, routine blood test and determination result of C-reactive protein (CRP). Results: The NGAL/HNL, CRP and WBC contents as well as percentage of neutrophil (NEUT) in sera of patients with bacterial infection were significantly higher than those with viral infection (each P < 0.01). The sensitivity and specificity of determination of NGAL/HNL content in sera for differential diagnosis of acute bacterial and viral infections were 84. 67% and 95% respectively, while the positive and negative predictive values as well as accuracy were higher than those of tests for CRP, WBC, NEUT and percentage of NEUT. Conclusion: NGAL/HNL showed high sensitivity and specificity in differential diagnosis of acute bacterial and viral infections as compared with other methods such as routine blood test and determination of CRP, indicating a higher capability in differential diagnosis.


Wu C.-Z.,208th Hospital of PLA | Li C.-M.,208th Hospital of PLA | Han S.,208th Hospital of PLA | Wu S.,208th Hospital of PLA
Chinese Journal of Cancer Research | Year: 2012

Extraskeletal osteosarcoma (EOS) is rare and commonly arises in the retroperitoneum, limbs, head and neck. There is no significant difference between EOS and other malignant tumors in soft tissue. Localized pain and swelling are the common presenting symptoms. Clinical diagnosis of EOS is difficult, imaging techniques may be helpful and careful, and the histopathological analysis is necessary. The common histological variants of EOS include: osteoblastoma, chondroblastoma, and fibroblastoma, and other unusual subtypes were reported occasionally. It should be distinguished with myositis ossificans, malignant mesenchymoma, giant cell tumor and parosteal osteosarcoma. We present an EOS arising in the penis. The primary site and histological category of the tumor were extremely rare. We hope the case will be helpful to the recognition of clinical signs, iconography and histopathology of EOS. © 2012 Chinese Anti-Cancer Association and Springer-Verlag Berlin Heidelberg.


Sheng C.-H.,208th Hospital of PLA | Bao F.,208th Hospital of PLA | Xu S.,208th Hospital of PLA
Journal of Practical Oncology | Year: 2011

Objective: To analyze the therapeutic effects of chemotherapy combined with dendritic cell-cytokine induced killers (DC-CIK) cells on non-small cell lung cancer(NSCLC). Methods: Sixty-five patients of metastatic NSCLC were randomized divided into two groups : the study group (chemotherapy combined with DC-CIK) and the control group (chemotherapy). The study group received NP chemotherapy regimen combined with DC-CIK cells, the control group was given NP chemotherapy regimen only. The short-time clinical therapeutic effect, life quality, immunal function, 3-year survival rate and security were observed. Results: There was no significant difference between two groups on short-time clinical effect. However, the further research pointed that in lung adenocarcinoma, the objective remission rates of the study group and the control group were 52.6% and 42.1%, respectively. 93.8% of KPS score in the study group rised, while that of the control group was 78.8%. In the study group the CD3+, CD8+ and CD56+ positive rate of T lymphocytes and the value of Ag-NORs in peripheral blood prominently rised. The 3-year survival rate of the study group was 25.0%, while that of the control group was 15.2%. No adverse influence on heart function, liver function, renal function and blood routine was observed in the study group. Conclusion: Chemotherapy combined with DC-CIK cells for NSCLC can improve life quality, enhance immune function, increase survival rate and comprehensive therapy.

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