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Changchun, China

Zhao W.-C.,208th Hospital of PLA | Hou Z.-H.,Hospital Office | Zhang Q.,208th Hospital of PLA | Zheng W.,Infirmary
Asian Pacific Journal of Tropical Medicine | Year: 2014

Objective: To study the various processes involved in transcellular transport (TT) of huperzine A alone or in combination with ginkgolide B in Caco-2 and Madin-Darby canine renal (MDCK) cell monolayer. Methods: The transepithelial passage was assayed in the apical-to-basolateral (AP to BL) direction and opposite direction (BL to AP) in both cell lines. The determination of huperzine A and ginkgolide B were performed by high performance liquid chromatography (HPLC). The passage rates of huperzine A and ginkgolide B were calculated. Bi-directional TT (absorption and secretion) were taken in huperzine A and ginkgolide B in Caco-2 and MDCK cell monolayer. Results: TT absorption and secretion kinetics of huperzine A and ginkgolide B across two cells existed at the same time. The passage rates of huperzine A were increased significantly with adding different concentrations of ginkgolide B. Conclusions: The compound preparations of HA in combination with GB for dementia caused by cerebral ischemic have synergistic effects on the pharmacodynamics, and improve the bioavailability through BBB. © 2014 Hainan Medical College. Source


Wang R.,Jilin University | Wang R.,Inner Mongolia University | Wang Y.,Inner Mongolia University | Zhang R.,Peoples Hospital of Inner Mongolia Autonomous Region | And 2 more authors.
Journal of Pediatric Surgery Case Reports | Year: 2014

Colonic perforation is an extremely rare complication following ventriculoperitoneal (VP) shunting. The common treatment is to remove the perforating catheter and replace it with a new one. Here we report a case of colonic perforation from VP shunting in a 2-year, 8-month-old boy who presented with the distal end of the shunt catheter protruding out of his anus. The distal catheter was removed via the anus and the perforation repaired transanally. The patient's postoperative course was uneventful. This case reminds us that we should not make another rush to perform a new shunt operation unless there are some manifestations of hydrocephalus. Potential mechanisms of migration and its management strategy are discussed. © 2014 The Authors. Source


Liu R.,PLA Fourth Military Medical University | Xu H.,University of California at Los Angeles | Wang G.,208th Hospital of PLA | Li J.,Xian Jiaotong University | And 4 more authors.
PLoS ONE | Year: 2013

Background: The pathogenesis of extraocular muscle (EOM) weakness in myasthenia gravis might involve a mechanism specific to the EOM. The aim of this study was to investigate characteristics of the EOM related to its susceptibility to myasthenia gravis. Methods: Female F344 rats and female Sprague-Dawley rats were assigned to experimental and control groups. The experimental group received injection with Ringer solution containing monoclonal antibody against the acetylcholine receptor (AChR), mAb35 (0.25 mg/kg), to induce experimental autoimmune myasthenia gravis, and the control group received injection with Ringer solution alone. Three muscles were analyzed: EOM, diaphragm, and tibialis anterior. Tissues were examined by light microscopy, fluorescence histochemistry, and transmission electron microscopy. Western blot analysis was used to assess marker expression and ELISA analysis was used to quantify creatine kinase levels. Microarray assay was conducted to detect differentially expressed genes. Results: In the experimental group, the EOM showed a simpler neuromuscular junction (NMJ) structure compared to the other muscles; the NMJ had fewer synaptic folds, showed a lesser amount of AChR, and the endplate was wider compared to the other muscles. Results of microarray assay showed differential expression of 54 genes in the EOM between the experimental and control groups. Conclusion: Various EOM characteristics appear to be related to the increased susceptibility of the EOM and the mechanism of EOM weakness in myasthenia gravis. © 2013 Liu et al. Source


Wang J.,Xiamen University | Cui W.,Ningbo University | Ye J.,Xiamen University | Ji S.,University of South China | And 5 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2012

Although therapeutic cellular angiogenesis is effective for chronic ischemia, the optimal mode of cellular administration is still under exploration. This study aimed to develop a cellular delivery system to enhance the perfusion and angiogenesis in the ischemic hind limb. Collagen scaffold (CS) was prepared, and for morphology and toxicity analysis, bone marrow-derived mesenchymal stem cells (BMSCs) were isolated, expanded, filtrated, and seeded onto CS to construct BMSCs-CS. The ischemic hind limbs of rabbit models were implanted with autologous BMSCs-CS, CS, and autologous BMSCs; the untreated ischemic or normal animals were considered as the ischemic or normal control groups. Oxygen saturation parameters were regularly measured to determine the perfusion in the extremities. Histological examinations with hematoxylin and eosin immunostaining against von Willebrand factor and smooth muscle (SM) α-actin were performed for capillary and mature vessel evaluation. CS was a multiporous structure without cytotoxicity. At several intervals, the oxygen saturation ratio (OSR) in normal control was the highest. The OSRs in BMSCs-CS and CS were higher than that in BMSCs and ischemic control (p < 0.05); the OSR in BMSCs-CS group was higher than that in CS at 6 and 8 weeks (p < 0.05). The capillaries in BMSCs-CS and CS were higher than that in CS, BMSCs, and the ischemic or normal control (p < 0.05). The mature vessels in BMSCs-CS were higher than that in CS, BMSCs, and the ischemic or normal control (p < 0.05). The autologous cellular delivery system proved to be an effective approach for improving higher ischemic hind limb perfusion and angiogenesis as opposed to cellular therapy alone. Copyright © 2012 Wiley Periodicals, Inc. Source


Yan L.,Jilin Institute of Architecture and Civil Engineering | Dong W.-Y.,208th Hospital of PLA | Bo W.,Jilin Institute of Architecture and Civil Engineering
Proceedings of the 2013 3rd International Conference on Intelligent System Design and Engineering Applications, ISDEA 2013 | Year: 2013

Bamboo has many good features, is an ideal material for the construction industry. The numerical simulation of multi-layer seismic frame structure of bamboo by hand shows that, for re-bamboo wood columns, typical of the two failure modes for the transverse deformation is too large can not continue to load and top of the fibers in the lateral pressure exploded; bamboo column can be restored in the unloaded more than 80% of deformation, has excellent elastic recovery; bamboo can withstand the load beam is actually controlled by the stiffness, not strength. © 2012 IEEE. Source

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