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Li W.,University of Sichuan | Liu Y.,University of Sichuan | Liu Y.,Jilin University | Cheng S.,208 Hospital of Peoples Liberation Army | Duan Y.,Sichuan University
RSC Advances | Year: 2017

Concomitant findings of acetone (ACE) and isopropanol (IPA) in blood and other biological samples have been reported in diabetic decedents and clinic cases, but exhaled IPA has rarely been studied in breath research. This study aimed to investigate expression of exhaled IPA in diabetes and further explore the correlations between exhaled IPA and ACE, and to evaluate diabetes diagnostic applicability of exhaled IPA in combination with ACE. Exhaled breath samples at one time point from 85 type 2 diabetic patients and 56 healthy controls, and from four healthy individuals after ketogenic diet experiments were analyzed by gas chromatography mass spectrometry coupled with solid phase micro-extraction technique. Concentrations of exhaled IPA in the diabetic group (mean 85.44 ppbv) were significantly higher than those in the healthy group (mean 17.99 ppbv, p < 0.001). Ketogenic diet experiments showed that both IPA and ACE levels were elevated after keto-meals when under fat-consuming metabolic states. And they shared a similar changing pace, even though there was no linear relationship between IPA and ACE in terms of concentrations. The Spearman Correlation Coefficient between exhaled IPA and ACE in diabetes was 0.66, which indicated that IPA and ACE were metabolically correlated. ROC curve analysis showed that IPA possessed promising discriminatory ability (AUC 0.86) with a sensitivity of 75.3% and a specificity of 85.7% for diabetes diagnosis. This research indicates that endogenously produced IPA is a valuable biomarker for noninvasive diabetes diagnosis in breathomics analysis. This work also addressed the speculation that IPA can be metabolized from ACE via the reversible action of alcohol dehydrogenase. © The Royal Society of Chemistry.

Zhang L.-B.,208 Hospital of Peoples Liberation Army | Yang H.-S.,Jilin University | Cao S.,208 Hospital of Peoples Liberation Army | Fan S.,208 Hospital of Peoples Liberation Army | And 2 more authors.
Tumor | Year: 2010

Objective: To evaluate the clinical value of percutaneous transhepatic cholangial drainage (PTCD) and percutaneous transhepatic cholangial stent (PTCS) implantation therapy for malignant biliary obstruction in the elderly patients. Methods: Fifty three patients over 60 years-old were treated with PTCS implantation (n=35), PTCD for internal-external drainage (n=11), or single external drainage (n=7). The imaging examination was performed for all the patients one week after surgery. The serum levels of total bilirubin (TBIL) and alanine aminotransferase (ALT) were detected before and after surgery. Rusults: Metallic stents were successfully implanted in 32 patients during the surgery, and 3 patients were implanted 5 to 7 days later after PTCS. Both total serum levels of TBIL and ALT were significantly decreased at one week after surgery (P<0.05). The mean survival periods were 11.5 months for stent implantation patients and 5.5 months for PTCD patients. Conclusion: PTCD and PTCS are easy to perform and safe and effective in the treatment of malignant biliary obstruction of elderly patients.

Li W.,University of Sichuan | Liu Y.,University of Sichuan | Lu X.,University of Sichuan | Huang Y.,University of Sichuan | And 3 more authors.
Journal of Breath Research | Year: 2015

Breath acetone is a known biomarker for diabetes mellitus in breath analysis. In this work, a cross-sectional study of breath acetone based on clinical metabolic disorders of type 2 diabetes mellitus (T2DM) was carried out. Breath acetone concentrations of 113 T2DM patients and 56 apparently healthy individuals were measured at a single time point. Concentrations varied from 0.22 to 9.41 ppmv (mean 1.75 ppmv) for T2DM, which were significantly higher than those for normal controls (ranged from 0.32 to 1.96 ppmv, mean 0.72 ppmv, p = 0.008). Observations in our work revealed that breath acetone concentrations elevated to different degrees, along with the abnormality of blood glucose, glycated hemoglobin (HbA1c), triglyceride and cholesterol. Breath acetone showed obviously positive correlations with blood ketone and urine ketone. Possible metabolic relations between breath acetone and diabetic disorders were also discussed. This work aimed at giving an overall assessment of breath acetone from the perspective of clinical parameters for type 2 diabetes. © 2015 IOP Publishing Ltd.

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