Yang Y.-M.,SickKids Research Institute |
Yang Y.-M.,University of Toronto |
Wang W.,Huazhong University of Science and Technology |
Wang W.,180th Hospital of PLA |
And 6 more authors.
Nature Communications | Year: 2014
Neurons convey information in bursts of spikes across chemical synapses where the fidelity of information transfer critically depends on synaptic input-output relationship. With a limited number of synaptic vesicles (SVs) in the readily releasable pool (RRP), how nerve terminals sustain transmitter release during intense activity remains poorly understood. Here we report that presynaptic K + currents evoked by spikes facilitate in a Ca 2+ -independent but frequency- and voltage-dependent manner. Experimental evidence and computer simulations demonstrate that this facilitation originates from dynamic transition of intermediate gating states of voltage-gated K + channels (Kvs), and specifically attenuates spike amplitude and inter-spike potential during high-frequency firing. Single or paired recordings from a mammalian central synapse further reveal that facilitation of Kvs constrains presynaptic Ca 2+ influx, thereby efficiently allocating SVs in the RRP to drive postsynaptic spiking at high rates. We conclude that presynaptic Kv facilitation imparts neurons with a powerful control of transmitter release to dynamically support high-fidelity neurotransmission. © 2014 Macmillan Publishers Limited. All rights reserved.
Guan D.-G.,180th Hospital of PLA |
Chen H.-M.,180th Hospital of PLA |
Liao S.-F.,180th Hospital of PLA |
Zhao T.-Z.,PLA Fourth Military Medical University
Molecular Medicine Reports | Year: 2015
Malignant gliomas, which comprise the most common type of primary malignant brain tumor, are associated with a poor prognosis and quality of life. Paclitaxel (Taxol) and temozolomide (TMZ) are Food and Drug Administration-approved anticancer agents, which are known to have therapeutic applications in various malignancies. However, similar to other chemotherapeutic agents, the development of resistance to TMZ and Taxol is common. The aim of the present study was to investigate the regulation of glucose metabolism by TMZ and Taxol in glioma cells. The results demonstrated that glioma cells exhibit decreased glucose uptake and lactate production in response to treatment with TMZ; however, glucose metabolism was increased in response to Taxol treatment. Following analysis of TMZ- and Taxol-resistant cell lines, it was reported that glucose metabolism was decreased in the TMZ-resistant cells, but was increased in the Taxol-resistant cells. Notably, a combination of TMZ and Taxol exerted synergistic inhibitory effects on Taxol-resistant glioma cells. However, the synergistic phenotype was not observed following treatment with a combination of 5-fluorouracil and Taxol. Furthermore, restoration of glucose metabolism by overexpression of glucose transporter 1 in Taxol-resistant cells resulted in regained resistance to Taxol. Therefore, the present study proposes a novel mechanism accounting for the synergistic effects of Taxol and TMZ co-treatment, which may contribute to the development of therapeutic strategies for overcoming chemoresistance in patients with cancer.
Fan J.-H.,180th Hospital of PLA |
Li X.-X.,180th Hospital of PLA |
Pan D.-P.,180th Hospital of PLA
International Journal of Ophthalmology | Year: 2010
AIM: To study the ultrastructure of corneas preserved by long-term cryopreservation with improved glycerol and to study the lamellar keratoplasty. METHODS: The corneas coated with sodium hyaluronate were long-term preserved in -45 °C glycerol. After 1 year, 15 cases accepted lamellar keratoplasty with the long-term preserved cornea, and the cornea taken transmission electron microscopy(TEM) examination. RESULTS: All surgeries were successful, and patients were followed up for 6 to 24 months. 13 grafts were transparent, 1 graft was translucent, and 1 graft was neovascularization. TEM examination showed that the ultrastructure of cornea was well-preserved. CONCLUSION; The improved long-term cryopreservation with glycerol could preserve some viability of cornea. It is a proper way of preservation that could be used for lamellar keratoplasty in clinic when there is no fresh corneas.
Zhou J.,180th Hospital of PLA |
Huang X.,180th Hospital of PLA |
Ren C.,180th Hospital of PLA |
Guo X.,180th Hospital of PLA
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology | Year: 2015
OBJECTIVE: To study the anatomic features of the gracilis muscle flap and its clinical application in tongue reconstruction.METHODS: Thirty gracilis muscles in 15 cadavers were dissected, and the morphometric parameter of the gracilis muscle and the origin, length, diameter of the vascular and nerve pedicle were observed and measured.RESULTS: The length of the total gracilis and the muscle belly was 41.8±3.9 cm and 31.0±3.6 cm respectively, and the width and thickness of the muscle was 3.2±0.7 cm and 1.7±0.2 cm respectively. The blood supply to the gracilis originated from multiple source vessles, and two to five pedicles supplied the gracilis from its deep surface and entered the muscle near its anterior border, of which the dominant pedicle and the first distal pedicle were consistent in their anatomy which originated from the profunda femoris vessels and the femoris vessels respectively. The length and external diameter of these vessels were suitable for microvascular anastomosis, while other vessel pedicles were neither consistent in their anatomy nor the length and external diamiter suitable for microvascular anastomosis. The nerve to the gracilis arose from the anterior division of the obturator nerve consistently and its length and external diameter was suitable for anastomosis with the hypoglossal nerve.CONCLUSIONS: Because of superficial location, excellent morphological match for tongue, consistency in its vascular and neural pedicle's anatomy and suitability of neurovascular pedicle's length and external diameter for microsurgical anastomoses, possibility of regaining tongue movements, the gracilis flap is a ideal option for tongue reconstruction.
Cheng J.-T.,180Th Hospital of PLA |
Wei Z.-Y.,180Th Hospital of PLA |
Liu D.-J.,180Th Hospital of PLA |
Zhang Y.,180Th Hospital of PLA |
And 3 more authors.
Chinese Journal of Burns | Year: 2013
Objective: To observe the effects of up- or down-regulation of haemoxygenase 1 (HO-1) gene expression on intestinal mucosa injury induced by intra-abdominal hypertension (IAH). Methods: (1) Reproduction of rat model of up- or down-regulation of HO-1 gene expression. Twenty-four healthy adult Wistar rats were divided into Co-PP (HO-1 specific revulsive) 2.5 mg, Co-PP 5.0 mg, Sn-PP (HO-1 specific inhibitor) 2.5 mg, and control groups according to the random number table, with six rats in each group. Rats in groups Co-PP 2.5 mg and Sn-PP 2.5 mg were respectively given Co-PP 2.5 mg/kg and Sn-PP 2.5 mg/kg by intraperitoneal injection, once every 12 hours for 3 days. The rats in group Co-PP 5.0 mg were intraperitoneally injected with Co-PP 5.0 mg/kg, once a day for 3 days. The rats in control group were treated with equal volume of normal saline by intraperitoneal injection. All rats were sacrificed on post injection day (PID) 4, and intestinal mucosa tissues were collected for determination of HO-1 mRNA expression. Optimal dose of Co-PP was chosen for the following experiment. (2) The influence of up- or down-regulation of HO-1 gene expression on intestinal mucosa injury under IAH condition. Another 24 healthy adult Wistar rats were divided into control, IAH, Co-PP + IAH, and Sn-PP + IAH groups according to the random number table, with six rats in each group. The rats in groups Co-PP + IAH and Sn-PP + IAH were intraperitoneally injected with 2.5 mg/kg Co-PP and 2.5 mg/kg Sn-PP, once every 12 hours for 3 days. Equal volume of normal saline was intraperitoneally injected into the rats in control group, once every 12 hours for 3 days. Then, nitrogen gas pneumoperitoneum was used to establish the model of IAH in rats of the latter three groups on PID 4, with IAP at 20 mm Hg (1 mm Hg = 0.133 kPa), and it was maintained for 2 hours. Puncture and intubation were performed in rats of control group without inflating nitrogen gas. Jejunal segment in the length of 10-15 cm was harvested for collecting intestinal mucosa tissues to determine the HO-1 mRNA expression and diamine oxidase (DAO) content. Serum obtained from portal vein blood was collected to determine the D-lactate, TNF-α, and IL-6 contents. Another jejunal segment in the length of 1-2 cm was harvested for histopathological examination. Data were processed with one-way analysis of variance and t test. Results: (1) The HO-1 mRNA expression in group Co-PP 2.5 mg was significantly higher than that in control and Co-PP5.0 mg groups (with t values respectively 4.756, 3.175, P < 0.05 or P < 0.01). The HO-1 mRNA expression in group Sn-PP 2.5 mg was significantly lower than that in control group (t = 4.880, P < 0.01). The optimal dose of Co-PP for the following experiment was 2.5 mg/kg. (2) HO-1 mRNA expression in group Co-PP + IAH was 60 ± 5, and it was obviously higher than that of group IAH (49 ± 5, t = 3.811, P < 0.01) and control group (39 ± 4, t = 8.034, P < 0.001). HO-1 mRNA expression was higher in group IAH than in control group (t = 3.826, P < 0.01). HO-1 mRNA expression in group Sn-PP + IAH was 29 ± 4, which was obviously lower than that of control group (t = 4.330, P < 0.01). The contents of DAO and D-lactate in group Co-PP + IAH were (0.52 ± 0.05) U/mL and (1.9 ± 0.6) mg/L, which were significantly lower than those in group IAH [ (0.88 ± 0.06) U/mL and (4.3 ± 0.7) mg/L, with t values respectively 11.291, 6.376, P values all below 0.01], but still higher than those in control group [(0.34 ± 0.04) U/mL, (1.2 ± 0.5) mg/L, with t values respectively 6.886, 2.295, P < 0.05 or P < 0.01]. The contents of TNF-α and IL-6 were much lower in group Co-PP + IAH than in group IAH, but still higher than in control group (with t values from 3.781 to 18.557, P values all below 0.01). The contents of DAO, D-lactate, TNF-α, and IL-6 in group Sn-PP + IAH were all higher than those in the other 3 groups (with t values from 4.181 to 32.938, P values all below 0.01). Structure of epithelial cells from intestinal mucosa was intact and regularly arranged in rats of control group. Intestinal mucosal tissue was edematous, and the top of villi was anabrotic and necrotic in rats of group IAH. Compared with that of group IAH, the degree of intestinal mucosa injury was alleviated in rats of group Co-PP + IAH, while the pathology was aggravated in rats of group Sn-PP + IAH. Conclusions: Up-regulation of HO-1 gene expression can ameliorate intestinal mucosa injury caused by IAH, thus protecting intestinal mucosa tissues.
Zhou J.-H.,180th Hospital of PLA |
Ren C.-Q.,180th Hospital of PLA
Journal of Cranio-Maxillofacial Surgery | Year: 2013
Introduction: We report functional and clinical outcomes following use of a preauricular long-corniform incision for open reduction and internal fixation (ORIF) of mandibular condylar fractures. Materials and methods: Patients with mandibular condylar fractures who underwent ORIF via a 120 preauricular long-corniform incision were included in the study. A total of 78 patients (100 condyles) were included. Follow-up occurred 10 days and 1-6 months after surgery, and included assessments of clinical, functional outcome, complications, and bone fusion. Results: There were 38 high neck, 26 low base, and 35 diacapitular condylar fractures. All measures of functional outcome significantly improved over time after surgery regardless of fracture type (all P < 0.001). The vast majority of patients in all fracture type groups had good occlusion (≥88.5%), no pain (≥89.5%), and anatomical reduction 10 days after surgery (≥81.6%). Fracture healing was complete in all patients after 6 months. There were no long-term complications and all patients were satisfied with their postoperative appearance. Conclusions: Our findings suggest that a preauricular long-corniform incision provides a good visual field during surgery, and allows for effective ORIF of mandibular high neck, low base, and diacapitular condylar fractures, with positive outcomes and minimal postoperative complications. © 2012 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.
Fan J.,180th Hospital of PLA |
Wang M.,180th Hospital of PLA |
Zhong F.,180th Hospital of PLA
BioMed Research International | Year: 2016
In our retrospective study we evaluated the efficacy of an improved amniotic membrane (AM) roll-in filling technique (AMR) combined with multilayer amniotic membrane cover to treat corneal perforation and included 46 cornea perforations ≤ 3 mm in diameter treated with AMR and 20% C3F8 mixed gas filling of the anterior chamber. Anterior chamber depth, aqueous leakage, bubble maintenance time, and cornea morphology were monitored after each operation. The mean diameter of corneal perforation was 1.60 ± 0.55 mm (range 0.5-3) and the success rate of the AMR method for corneal perforation reconstruction was 100% after a single operation. Anterior chamber depth was normally reconstructed without AMR break-off, aqueous leak, or other complications. The mean time of the C3F8 gas bubble in the anterior chamber was 8.6 ± 2.0 days (range 4-12). At the last follow-up, all patients' visual acuity was improved to varying degrees. The mean follow-up time was 11.0 ± 5.6 months (range 3-36). The AMR plugging combined with multilayer AM cover is a secure and easy intervention, which led to 100% success in our study. Various perforations ranging from trauma to infection can be treated with AMR, which is especially practical in those countries where donor cornea availability is limited. © 2016 Junhua Fan et al.
Wang Y.,Capital Medical University |
Mo L.,180th Hospital of PLA |
Wei W.,Capital Medical University |
Shi X.,Capital Medical University
International Journal of Nanomedicine | Year: 2013
Human uveal melanoma is the most common primary intraocular tumor, and brachytherapy is one of the most common and effective treatment strategies. In order to find a safer and more effective way to increase the radio sensitivity of the tumor, we tried to use the dendrimer nanoparticle performing coexpression gene radiotherapy. In this study, we constructed recombinant DNA plasmids (early growth response-1 tumor necrosis factor-α [pEgr1-TNFα], pEgr1 thymidine kinase [TK], and pEgr1-TNFα -TK) according to the Egr1 promoter sequence. The sequences of human TNFα and herpes simplex virus (HSV) TK that were published by GenBank. Agarose gel electrophoresis and DNA sequencing had proven that we constructed the double-gene recombined plasmids pEgr1-TNF-TK correctly, as well as the plasmids pEgr1-TNFα and pEgr1-TK. The dendrimer nanoparticles combined with plasmid DNA as dendriplexes were verified with agarose gel electrophoresis and observed by transmission electron microscopy (TEM) and scanning electron microscopy to define size and shape. Zeta potential was measured using a Zetasizer analyzer. Optimal size and neutral zeta-potential characteristics of dendriplexes were achieved for the transfection studies. DNase I examination proved that the dendriplexes could protect plasmid DNA for at least 6 hours. The recombinant plasmids were transfected with dendrimer nanoparticles into the human choroidal melanoma OCM-1 cell line, followed by exposure to iodine-125 (125I) after transfection. After transfection with dendrimer nanoparticles and the irradiation of 125I, the gene expressions of TNFα and HSV1-TK were significantly increased at the protein level by enzyme-linked immunosorbent assay and Western blot analysis in OCM-1 cells. The cellular morphology of OCM-1 cells altering was observed by TEM, and a decrease in cell proliferation was revealed in cell-growth curves. Flow cytometry of annexin V/propidium iodide double-dyeing apoptosis and caspase-3 fluorescence staining showed that this treatment method could turn transfected OCM-1 cells into apoptosis and necrosis by the effects of the gene expression. This study indicated that the dendrimer nanoparticles with coexpression of TNF-α and HSV1-TK gene therapy are effective and safe and can provide us with a novel strategy to treat human uveal melanoma in the future. © 2013 Wang et al.
Hong Q.,180th Hospital of PLA |
Fang J.,180th Hospital of PLA |
Pang Y.,180th Hospital of PLA |
Zheng J.,180th Hospital of PLA
Medical Oncology | Year: 2014
The aim of this study was to facilitate and deepen the understanding of the associations of the microRNA-29 (miR-29) family with tumor progression and patients' prognosis of primary osteosarcoma. We examined expression levels of miR-29a, miR-29b, and miR-29c in tumor tissues and patients' sera of 80 cases of primary osteosarcomas by quantitative real-time reverse transcriptase- polymerase chain reaction. The correlations of their serum levels with clinicopathological characteristics and patient prognosis were also analyzed. The expression levels of miR-29a, miR-29b, and miR-29c in osteosarcoma tissues and patients' sera were all significantly higher than those in normal controls (all P < 0.05). The serum levels of miR-29a and miR-29b in the patients with higher tumor grade (both P = 0.01), positive metastasis (both P = 0.006), and positive recurrence (both P = 0.006) were both markedly higher than those with lower tumor grade, negative metastasis, and negative recurrence. According to the survival analysis of 80 osteosarcoma patients, cases in the miR-29a-high and miR- 29b-high-expression groups both showed shorter overall survival (OS, both P < 0.001) and disease-free survival (DFS, both P < 0.001). Furthermore, the serum levels of miR-29a and miR-29b were both independent prognostic factors for OS and DFS of osteosarcoma patients. However, high miR-29c level was not related to any clinicopathological characteristics and patient prognosis of osteosarcomas (P > 0.05). The findings from the present study reveal that the miR-29 family may play crucial roles in the development and progression of human osteosarcoma. In particular, the serum levels of miR-29a and miR-29b may well estimate the prognosis of patients with this malignancy. © Springer Science+Business Media 2014.
PubMed | 180th Hospital of PLA
Type: Journal Article | Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology | Year: 2015
To study the anatomic features of the gracilis muscle flap and its clinical application in tongue reconstruction.Thirty gracilis muscles in 15 cadavers were dissected, and the morphometric parameter of the gracilis muscle and the origin, length, diameter of the vascular and nerve pedicle were observed and measured.The length of the total gracilis and the muscle belly was 41.83.9 cm and 31.03.6 cm respectively, and the width and thickness of the muscle was 3.20.7 cm and 1.70.2 cm respectively. The blood supply to the gracilis originated from multiple source vessles, and two to five pedicles supplied the gracilis from its deep surface and entered the muscle near its anterior border, of which the dominant pedicle and the first distal pedicle were consistent in their anatomy which originated from the profunda femoris vessels and the femoris vessels respectively. The length and external diameter of these vessels were suitable for microvascular anastomosis, while other vessel pedicles were neither consistent in their anatomy nor the length and external diamiter suitable for microvascular anastomosis. The nerve to the gracilis arose from the anterior division of the obturator nerve consistently and its length and external diameter was suitable for anastomosis with the hypoglossal nerve.Because of superficial location, excellent morphological match for tongue, consistency in its vascular and neural pedicles anatomy and suitability of neurovascular pedicles length and external diameter for microsurgical anastomoses, possibility of regaining tongue movements, the gracilis flap is a ideal option for tongue reconstruction.