Ye Z.,161 Hospital of PLA |
Jingzhong L.,161 Hospital of PLA |
Yangbo L.,Wenzhou University |
Lei C.,Wenzhou University |
Jiandongt Y.,Wenzhou University
Oncology Research | Year: 2014
Propofol is one of the extensively commonly used intravenous anesthetic agents. Previous studies have indicated that propofol has the ability to influence the biological behavior of several human cancer cells. However, the effect of propofol on osteosarcoma and its related molecular mechanisms are still not clear. Here we found that propofol significantly elevated the expression of miR-143, inhibited cell proliferation and invasion, and promoted apoptosis in osteosarcoma cell line MG63, Propofol also efficiently decreased protein expression of matrix metalloproteinase 13 (MMP-13). Moreover, the overexpression of miR-143 decreased MMP-13 protein level. Finally, the neutralization of miR-143 by anti-miR-143 antibody reversed the effect of propofol on cell proliferation, apoptosis, and invasion and unregulated MMP-13 expression in MG63 cells. Taken together, propofol may have antitumor potential in osteosarcoma, which is partly due to the downregulalion of MMP-13 expression by miR-143. Copyright © 2014 Cognizant Comm. Corp.
Chen X.-H.,Wuhan University |
Chen X.-H.,161 Hospital of PLA |
Yu X.-Y.,Xiaogan Central Hospital |
Zhou J.,Xiaogan Central Hospital |
And 4 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2014
Objective To identify the pathogenic gene in a non-syndromic deafness family, provide an accurate genetic consultation and early intervention for deaf family to reduce the incidence of congenital deafness. Methods Mutation analysis was carried out by polymerase chain reaction followed by DNA sequencing of coding region of GJB2 gene. The fetal DNA was extracted from the amniotic fluid cells by amniocentesis at 20 weeks during pregnancy. The genotype of the fetus was characterized for predicting the status of hearing. Results Complex heterozygous mutations 235delC and 176-191del16bp were detected in the proband of the family, heterozygous mutation 176-191del16bp was detected in the father, and 235delC was detected in the mother. Fetus carried 235delC heterozygous mutation inherited from his mother. Conclusions The proband's hearing loss is resulted from the complex heterozygous mutations 235delC and 176-191del16bp in GJB2 gene. Fetus is a heterozygous mutation 235delC carrier. Prenatal diagnosis for deafness assisted by genetic test can provide efficient guidance about offspring's hearing condition, and prevent another deaf-mute member from birth.
Cheng X.,PLA Fourth Military Medical University |
Guan S.,PLA Fourth Military Medical University |
Lu H.,PLA Fourth Military Medical University |
Zhao C.,PLA Fourth Military Medical University |
And 6 more authors.
Lasers in Surgery and Medicine | Year: 2012
Background and Objective In recent years, various laser systems have been introduced into the field of laser-assisted endodontic therapy. The aim of this study was to evaluate the bactericidal effect of Nd:YAG, Er:YAG, Er,Cr:YSGG laser radiation, and antimicrobial photodynamic therapy (aPDT) in experimentally infected root canals compared with standard endodontic treatment of 5.25% sodium hypochlorite (NaClO) irrigation. Materials and Methods Two hundred and twenty infected root canals from extracted human teeth (contaminated with Enterococcus faecalis ATCC 4083 for 4 weeks) were randomly divided into five experimental groups (Nd:YAG, Er:YAG + 5.25% NaClO + 0.9% normal saline + distilled water (Er:YAG/NaClO/NS/DW), Er:YAG + 0.9% normal saline + distilled water (Er:YAG/NS/DW), Er,Cr:YSGG, and aPDT) and two control groups (5.25% NaClO as positive control and 0.9% normal saline (NS) as negative control). The numbers of bacteria on the surface of root canal walls and at different depths inside dentinal tubules before and after treatment were analyzed by means of one-way analysis of variance (one-way ANOVA). The morphology of bacterial cells before and after treatment was examined by scanning electron microscopy (SEM). Results After treatment, the bacterial reductions in the experimental groups and the positive control group were significantly greater than that of the negative control group (P < 0.001). However, only Er:YAG/NaClO/NS/DW group showed no bacterial growth (the bacterial reduction reached up to 100%) on the surface of root canal walls or at 100/200 μm inside the dentinal tubules. Conclusions All the laser radiation protocols tested, especially Er:YAG/NaClO/NS/DW, have effective bactericidal effect in experimentally infected root canals. Er:YAG/NaClO/NS/DW seems to be an ideal protocol for root canal disinfection during endodontic therapy. Copyright © 2012 Wiley Periodicals, Inc.
Yao J.,161 Hospital of PLA |
Chen Y.,Chinese PLA General Hospital |
Wang Y.,Chinese PLA General Hospital |
Liu S.,253 Hospital of PLA |
And 3 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014
The clear cell renal cell carcinoma (ccRCC) is the most common subtype in renal cell carcinomas. Rapidly accumulating studies show that the long non-coding RNAs (lncRNAs) may play essential roles in cancers. In this study, we investigated the expression pattern of a novel lncRNA CADM1-AS1 in ccRCC by quantitative real time PCR. The results showed that CADM1-AS1 expression was down-regulated in tumor tissues in 64 patients with ccRCC compared with adjacent non-tumor tissues. Furthermore, the expression of CADM1-AS1 was positively correlated with the expression of mRNA CADM1 in ccRCC specimens (R = 0.611, P <0.0001). Decreased CADM1-AS1 expression was correlated with the progression of AJCC stage (P = 0.039) and worse survival of ccRCC patients (P <0.05). Also, multivariate analysis identiied low CADM1-AS1 expression as an independent prognostic factor for ccRCC (P <0.001, HR = 0.211, 95% CI = 0.088-0.504). In addition, we used small interfering RNA (siRNA) to evaluate the biological function of CADM1-AS1 in vitro. The results showed that CADM1-AS1 expression was positively associated with CADM1 mRNA expression in 786-O cells and ACHN cells. Functional experiments demonstrated markedly enhanced ability of growth and migration, and reduced apoptotic rate in CADM1-AS1 knocking down in 786-O cells. Conversely, overexpression of CADM1-AS1 showed a signiicant decrease in growth and migration, along with an increase in apoptotic rate in ACHN cells. In conclusion, our data demonstrated CADM1-AS1 is a new tumor suppressor in ccRCC which regulates cell proliferation, apoptosis and migration via the expression pattern of "CADM1-AS1/CADM1 mRNA gene pairs". CADM1-AS1 may be a potential biomarker and therapeutic target in patients with ccRCC.
Qin X.,Chinese PLA General Hospital |
Qin X.,253 Hospital of PLA |
Yao J.,161 Hospital of PLA |
Yao J.,Chinese PLA General Hospital |
And 4 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014
Growing evidence demonstrates that long non coding RNAs (lncRNAs) play an important role in cancer origination and progression. A novel lncRNA, TSLC1-AS1, is the antisense transcript of tumor suppressor TSLC1. The expression profile and function of TSLC1-AS1 in glioma were investigated using Real-Time Quantitative PCR and siRNA knockdown. The data showed that TSLC1-AS1 expression was down-regulated in tumor tissues compared with that in adjacent normal tissues, and negatively associated with the WHO criteria of the tumors. Overexpression of TSLC1-AS1 resulted in up-regulation of TSLC1 and significant inhibition of cell proliferation, migration and invasion in U87 cells, while knockdown of TSLC1-AS1 in SNB-19 cells showed the opposite effect. The expression of TSLC1-AS1 was also positively correlated with other tumor suppressors NF1, VHL, PIK3R1 and negatively correlated with the oncogene BRAF. The results suggested that TSLC1-AS1 was a tumor suppressor of glioma and a mediator of TSLC1 expression. LncRNA TSLC1-AS1 may serve as a potential biomarker and therapeutic target for glioma.
Zeng X.-T.,Hubei University of Medicine |
Leng W.-D.,Hubei University of Medicine |
Zhang C.,Hubei University of Medicine |
Liu J.,161 Hospital of PLA |
And 2 more authors.
Oral Oncology | Year: 2015
Background: Epidemiological studies have focused on the association between toothbrushing and head and neck cancer (HNC). However, the question of whether toothbrushing is associated with decreased risk of HNC remains unanswered. Since there is currently no systematic review or meta-analysis available to provide quantitative findings on this important clinical question; we consequently performed this meta-analysis to investigate the association between toothbrushing and HNC risk. Methods: We searched PubMed and Embase up to January 13 (updated on October 20), 2014 to identify observational studies that investigated the association between toothbrushing and HNC. After study section and data extraction, the meta-analysis was conducted using RevMan 5.2 software. Results: A total of 18 case-control studies involving 7068 cases and 9990 controls were included. The meta-analysis showed that compared with highest toothbrushing frequency, lowest level was significantly increased risk of HNC 2.08 times (odds ratio = 2.08, 95% confidence interval = 1.65-2.62). This significant association remained consistent after adjusting for smoking status and alcohol consumption. No publication bias was detected. Conclusions: This meta-analysis found frequency of toothbrushing was significantly associated with HNC risk. Effective toothbrushing may be potentially important for the prevention of HNC and we suggest that the frequency be twice per day (morning and night). © 2015 Elsevier Ltd. All rights reserved.
Ma T.,Hubei University of Medicine |
Ma Z.-Q.,161 Hospital of PLA |
Du X.-H.,161 Hospital of PLA |
Yu Q.-S.,161 Hospital of PLA |
And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015
Objective: To study the effect of valsartan on ACAT-1 and PPAR-γ expression after vascular endothelial balloon injury in intimal hyperplasia process. Methods: 24 male New Zealand white rabbits were randomly divided into three groups with 8 in each group. Control group: rabbits were fed with normal diet; Balloon injury group: rabbits were fed with 0.5% cholesterol, 5% lard rabbit feed; balloon injury + valsartan group, rabbits were fed with 0.5% cholesterol, 5% lard rabbit feed added with 10 mg/(kg.d) valsartan gavage. RT-PCR and Western blotting method were used to detect the carotid ACAT-1, PPAR-γ mRNA and protein expression after 8 weeks of feeding. Results: In carotid artery balloon injury group, vascular smooth muscle cells (VSMC) proliferation and intimal hyperplasia were significantly higher 14 d after endothelial injury. In 14 days valsartan treatment group VSMC proliferation and intimal hyperplasia were lighter than the surgery group. Compared with the control group, ACAT-1, PPAR-γ mRNA and protein were significantly increased in balloon injury group and valsartan group (P < 0.01 or P < 0.05); the expression of ACAT-1 mRNA and protein were significantly lower in valsartan group and balloon injury group (P < 0.01 or P < 0.05). The expression of PPAR-γ mRNA and protein in valsartan group expression was significantly higher than that in the balloon injury group (P < 0.05). The expression level of ACAT-1 and PPAR-γ mRNA in balloon injury group and valsartan group showed negative correlation (P < 0.05). Conclusion: The expression of ACAT-1, PPAR-γ mRNA and protein content were significantly increased in intimal hyperplasia process after vascular endothelial balloon injury. The effect of valsartan suppressed intimal hyperplasia correlated with the expression of down-regulated ACAT-1 and up-regulated PPAR-γ. © 2015, E-Century Publishing Corporation. All rights reserved.
Tao Y.,161 Hospital of PLA |
Chen J.,161 Hospital of PLA
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2016
OBJECTIVE: To explore the role of SIRT3 in regulating the proliferation of hepatocellular carcinoma (HCC) cells in vitro.METHODS: The protein expression of SIRT3 in 2 normal liver tissues, 2 immortalized hepatocyte lines, and 3 HCC cell lines was determined with Western blotting. SIRT3 overexpression and knockdown in HCC cells were induced by transfection with a vector expressing SIRT3 and a siRNA construct targeting SIRT3, respectively. The efficiency of SIRT3 overexpression and knockdown was detected by Western blot and qRT-PCR, respectively. The proliferation of the transfected HCC cells was examined using Trypan blue exclusion assay, and the cellular DNA synthesis was tested using EdU incorporation assay. The colony-forming ability of the cells was analyzed by colony formation assays.RESULTS: SIRT3 expression was significantly lower in the 3 HCC cell lines than in immortalized hepatocytes and normal liver tissues. SIRT3 overexpression in HCC cells significantly lowered the cell proliferation by 51%-61% (P<0.001), reduced cellular DNA synthesis by 57% (P<0.05), and inhibited colony formation of the cells. SIRT3 knockdown significantly increased the proliferation of HCC cells by 51%-61% (P<0.01) and enhanced DNA synthesis by 137%-149% (P<0.01).CONCLUSIONS: SIRT3 plays a inhibitory role in regulating the proliferation of HCC cells in vitro.
Li Z.,161 Hospital of PLA |
Qi J.-Y.,Huazhong University of Science and Technology |
Guo W.,Huazhong University of Science and Technology |
Guo H.,Huazhong University of Science and Technology
Medical Journal of Chinese People's Liberation Army | Year: 2013
Objective To analyze the etiology, clinical characteristics and diagnostic methods of fever of unknown origin (FUO), so as to provide a reference for the diagnosis and treatment of FUE. Methods The clinical data of 340 patients with FUO admitted in Tongji Hospital from Jan. 2005 to Dec. 2010 were retrospectively analyzed. The relationship between etiology and gender, age of the patients, and course of fever were comprehensively evaluated, and the diversity of disease spectrum was also analyzed. Results Among the 340 patients analyzed, 295(86.76%) the diagnosis was definitely made. As to the etiology, 150(50.85%) were suffering from infectious diseases, 145(49.15%) non-infectious diseases, and in 45(13.24%) the etiology remained unknown till they were discharged from hospital. Among all of the patients, 186(54.71%) were male and 154(45.29%) were female. No significant difference existed in the ratio of disease distribution between male and female patients, but the incidence of connective tissue disease was significantly higher in female patients than in male patients, while the incidence of lymphoma was significantly higher in male patients than in females. As to the age distribution of the disease, 66 patients (19.41%) were younger than 20 years old, 98 patients (28.82%) were 21-40, 136(40.00%) were 41-60, and 40(11.76%) were older than 60. The mean age of patients suffering from solid tumors was the highest (48.4 ± 17.2 years), and that of adult Still disease was youngest (27.4 ± 11.9 years). The course of fever in 202 patients (59.41%) was shorter than 4 weeks, that of 50 patients (14.71%) was 4-8 weeks, and in 88 patients (25.88%) it was longer than 8 weeks. Febrile course was longest in patients suffering from connective tissue disease, lymphoma and tuberculosis. Conclusions Most of the patients with FUO could be definitely diagnosed prudent inquiry of history, followed by scrupulous physical examination, aided by appropriate instrumental examination, most cases of FUO could be definitely diagnosis. Among these patients, infectious diseases remain the main causes of FUO. The disease spectrum will be diverse due to different gender, age and course of fever.
PubMed | 161 Hospital of PLA
Type: Journal Article | Journal: Oncology research | Year: 2014
Propofol is one of the extensively commonly used intravenous anesthetic agents. Previous studies have indicated that propofol has the ability to influence the biological behavior of several human cancer cells. However, the effect of propofol on osteosarcoma and its related molecular mechanisms are still not clear. Here we found that propofol significantly elevated the expression of miR-143, inhibited cell proliferation and invasion, and promoted apoptosis in osteosarcoma cell line MG63. Propofol also efficiently decreased protein expression of matrix metalloproteinase 13 (MMP-13). Moreover, the overexpression of miR-143 decreased MMP-13 protein level. Finally, the neutralization of miR-143 by anti-miR-143 antibody reversed the effect of propofol on cell proliferation, apoptosis, and invasion and upregulated MMP-13 expression in MG63 cells. Taken together, propofol may have antitumor potential in osteosarcoma, which is partly due to the downregulation of MMP-13 expression by miR-143.