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Zeng X.-T.,Hubei University of Medicine | Leng W.-D.,Hubei University of Medicine | Zhang C.,Hubei University of Medicine | Liu J.,161 Hospital of PLA | And 2 more authors.
Oral Oncology | Year: 2015

Background: Epidemiological studies have focused on the association between toothbrushing and head and neck cancer (HNC). However, the question of whether toothbrushing is associated with decreased risk of HNC remains unanswered. Since there is currently no systematic review or meta-analysis available to provide quantitative findings on this important clinical question; we consequently performed this meta-analysis to investigate the association between toothbrushing and HNC risk. Methods: We searched PubMed and Embase up to January 13 (updated on October 20), 2014 to identify observational studies that investigated the association between toothbrushing and HNC. After study section and data extraction, the meta-analysis was conducted using RevMan 5.2 software. Results: A total of 18 case-control studies involving 7068 cases and 9990 controls were included. The meta-analysis showed that compared with highest toothbrushing frequency, lowest level was significantly increased risk of HNC 2.08 times (odds ratio = 2.08, 95% confidence interval = 1.65-2.62). This significant association remained consistent after adjusting for smoking status and alcohol consumption. No publication bias was detected. Conclusions: This meta-analysis found frequency of toothbrushing was significantly associated with HNC risk. Effective toothbrushing may be potentially important for the prevention of HNC and we suggest that the frequency be twice per day (morning and night). © 2015 Elsevier Ltd. All rights reserved. Source

Li Z.,161 Hospital of PLA | Qi J.-Y.,Huazhong University of Science and Technology | Guo W.,Huazhong University of Science and Technology | Guo H.,Huazhong University of Science and Technology
Medical Journal of Chinese People's Liberation Army | Year: 2013

Objective To analyze the etiology, clinical characteristics and diagnostic methods of fever of unknown origin (FUO), so as to provide a reference for the diagnosis and treatment of FUE. Methods The clinical data of 340 patients with FUO admitted in Tongji Hospital from Jan. 2005 to Dec. 2010 were retrospectively analyzed. The relationship between etiology and gender, age of the patients, and course of fever were comprehensively evaluated, and the diversity of disease spectrum was also analyzed. Results Among the 340 patients analyzed, 295(86.76%) the diagnosis was definitely made. As to the etiology, 150(50.85%) were suffering from infectious diseases, 145(49.15%) non-infectious diseases, and in 45(13.24%) the etiology remained unknown till they were discharged from hospital. Among all of the patients, 186(54.71%) were male and 154(45.29%) were female. No significant difference existed in the ratio of disease distribution between male and female patients, but the incidence of connective tissue disease was significantly higher in female patients than in male patients, while the incidence of lymphoma was significantly higher in male patients than in females. As to the age distribution of the disease, 66 patients (19.41%) were younger than 20 years old, 98 patients (28.82%) were 21-40, 136(40.00%) were 41-60, and 40(11.76%) were older than 60. The mean age of patients suffering from solid tumors was the highest (48.4 ± 17.2 years), and that of adult Still disease was youngest (27.4 ± 11.9 years). The course of fever in 202 patients (59.41%) was shorter than 4 weeks, that of 50 patients (14.71%) was 4-8 weeks, and in 88 patients (25.88%) it was longer than 8 weeks. Febrile course was longest in patients suffering from connective tissue disease, lymphoma and tuberculosis. Conclusions Most of the patients with FUO could be definitely diagnosed prudent inquiry of history, followed by scrupulous physical examination, aided by appropriate instrumental examination, most cases of FUO could be definitely diagnosis. Among these patients, infectious diseases remain the main causes of FUO. The disease spectrum will be diverse due to different gender, age and course of fever. Source

Yao J.,161 Hospital of PLA | Chen Y.,Chinese PLA General Hospital | Wang Y.,Chinese PLA General Hospital | Liu S.,253 Hospital of PLA | And 3 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014

The clear cell renal cell carcinoma (ccRCC) is the most common subtype in renal cell carcinomas. Rapidly accumulating studies show that the long non-coding RNAs (lncRNAs) may play essential roles in cancers. In this study, we investigated the expression pattern of a novel lncRNA CADM1-AS1 in ccRCC by quantitative real time PCR. The results showed that CADM1-AS1 expression was down-regulated in tumor tissues in 64 patients with ccRCC compared with adjacent non-tumor tissues. Furthermore, the expression of CADM1-AS1 was positively correlated with the expression of mRNA CADM1 in ccRCC specimens (R = 0.611, P <0.0001). Decreased CADM1-AS1 expression was correlated with the progression of AJCC stage (P = 0.039) and worse survival of ccRCC patients (P <0.05). Also, multivariate analysis identiied low CADM1-AS1 expression as an independent prognostic factor for ccRCC (P <0.001, HR = 0.211, 95% CI = 0.088-0.504). In addition, we used small interfering RNA (siRNA) to evaluate the biological function of CADM1-AS1 in vitro. The results showed that CADM1-AS1 expression was positively associated with CADM1 mRNA expression in 786-O cells and ACHN cells. Functional experiments demonstrated markedly enhanced ability of growth and migration, and reduced apoptotic rate in CADM1-AS1 knocking down in 786-O cells. Conversely, overexpression of CADM1-AS1 showed a signiicant decrease in growth and migration, along with an increase in apoptotic rate in ACHN cells. In conclusion, our data demonstrated CADM1-AS1 is a new tumor suppressor in ccRCC which regulates cell proliferation, apoptosis and migration via the expression pattern of "CADM1-AS1/CADM1 mRNA gene pairs". CADM1-AS1 may be a potential biomarker and therapeutic target in patients with ccRCC. Source

Qin X.,Chinese PLA General Hospital | Yao J.,161 Hospital of PLA | Yao J.,Chinese PLA General Hospital | Geng P.,Chinese PLA General Hospital | And 3 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014

Growing evidence demonstrates that long non coding RNAs (lncRNAs) play an important role in cancer origination and progression. A novel lncRNA, TSLC1-AS1, is the antisense transcript of tumor suppressor TSLC1. The expression profile and function of TSLC1-AS1 in glioma were investigated using Real-Time Quantitative PCR and siRNA knockdown. The data showed that TSLC1-AS1 expression was down-regulated in tumor tissues compared with that in adjacent normal tissues, and negatively associated with the WHO criteria of the tumors. Overexpression of TSLC1-AS1 resulted in up-regulation of TSLC1 and significant inhibition of cell proliferation, migration and invasion in U87 cells, while knockdown of TSLC1-AS1 in SNB-19 cells showed the opposite effect. The expression of TSLC1-AS1 was also positively correlated with other tumor suppressors NF1, VHL, PIK3R1 and negatively correlated with the oncogene BRAF. The results suggested that TSLC1-AS1 was a tumor suppressor of glioma and a mediator of TSLC1 expression. LncRNA TSLC1-AS1 may serve as a potential biomarker and therapeutic target for glioma. Source

Ma T.,Hubei University of Medicine | Ma Z.-Q.,161 Hospital of PLA | Du X.-H.,161 Hospital of PLA | Yu Q.-S.,161 Hospital of PLA | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Objective: To study the effect of valsartan on ACAT-1 and PPAR-γ expression after vascular endothelial balloon injury in intimal hyperplasia process. Methods: 24 male New Zealand white rabbits were randomly divided into three groups with 8 in each group. Control group: rabbits were fed with normal diet; Balloon injury group: rabbits were fed with 0.5% cholesterol, 5% lard rabbit feed; balloon injury + valsartan group, rabbits were fed with 0.5% cholesterol, 5% lard rabbit feed added with 10 mg/(kg.d) valsartan gavage. RT-PCR and Western blotting method were used to detect the carotid ACAT-1, PPAR-γ mRNA and protein expression after 8 weeks of feeding. Results: In carotid artery balloon injury group, vascular smooth muscle cells (VSMC) proliferation and intimal hyperplasia were significantly higher 14 d after endothelial injury. In 14 days valsartan treatment group VSMC proliferation and intimal hyperplasia were lighter than the surgery group. Compared with the control group, ACAT-1, PPAR-γ mRNA and protein were significantly increased in balloon injury group and valsartan group (P < 0.01 or P < 0.05); the expression of ACAT-1 mRNA and protein were significantly lower in valsartan group and balloon injury group (P < 0.01 or P < 0.05). The expression of PPAR-γ mRNA and protein in valsartan group expression was significantly higher than that in the balloon injury group (P < 0.05). The expression level of ACAT-1 and PPAR-γ mRNA in balloon injury group and valsartan group showed negative correlation (P < 0.05). Conclusion: The expression of ACAT-1, PPAR-γ mRNA and protein content were significantly increased in intimal hyperplasia process after vascular endothelial balloon injury. The effect of valsartan suppressed intimal hyperplasia correlated with the expression of down-regulated ACAT-1 and up-regulated PPAR-γ. © 2015, E-Century Publishing Corporation. All rights reserved. Source

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