117th Hospital of PLA

Hangzhou, China

117th Hospital of PLA

Hangzhou, China

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Lu Q.,PLA Fourth Military Medical University | Lu S.,Xian Central Hospital | Huang L.,PLA Fourth Military Medical University | Wang T.,PLA Fourth Military Medical University | And 5 more authors.
Diagnostic Pathology | Year: 2013

Objective: This article aims to investigate the expression of vacuolar-H + -ATPase (V-ATPase) in non-small cell lung cancer (NSCLC) and its variations with pathological type and grade. Furthermore, to evaluate the chemotherapy drug sensitivity of different cancer tissues as well as its correlation with V-ATPase expression in NSCLC.Methods: V-ATPase expression was examined in 92 NSCLC tissue samples using the immunohistochemical Envision method and immunofluorescence assay. The location of V-ATPase expression was observed by confocal laser scanning microscopy and the difference of its expression rate was evaluated. The sensitivity of cancer tissues to chemotherapy drug was examined using MTT assay and its correlation with the V-ATPase expression was tested in NSCLC by Spearman rank correlation analysis.Results: V-ATPase expression was mainly localized in the cell membrane and cytoplasm. The expression rate of V-ATPase was 71.43% in squamous cell lung cancer, significantly lower than that of the lung adenocarcinoma (83.72%, P = 0.000). In different pathological grades of squamous cell lung cancer, the expression rate of V-ATPase was 58.33% in grade II, significantly lower than that of the grade III (84.00%, P = 0.014). The expression rate of V-ATPase in grade II lung adenocarcinoma was 76.67%, significantly lower than that of the grade ΙΙΙ adenocarcinoma (100.0%, P = 0.012). Correlation analysis showed that the sensitivity of NSCLC tissues to cyclophosphamide, gemcitabine, doxorubicin, paclitaxel and cisplatin was significantly correlated with the V-ATPase expression rate (P < 0.05).Conclusions: V-ATPase was overexpressed in NSCLC. The expression of V-ATPase was related to the pathological type and grade of cancer and was likely associated with chemotherapy drug resistance in NSCLC.Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7515811511020000. © 2013 Lu et al.; licensee BioMed Central Ltd.


Chen Q.-Y.,117th Hospital of PLA | Shi J.-G.,117th Hospital of PLA | Yao Q.-H.,Medicine University | Jiao D.-M.,117th Hospital of PLA | And 6 more authors.
Molecular and Cellular Biochemistry | Year: 2012

We previously reported that curcumin inhibited lung cancer A549 cells growth and promoted cell apoptosis in vitro. In this study, we further examined the apoptosis-related parameters, including lysosomal damage and cathepsin activation, in A549 cells exposed to curcumin. We found that curcumin caused lysosomal membrane permeabilization (LMP) and cytosolic relocation of cathepsin B (cath B) and cathepsin D (cath D). However, only Z-FA-fmk (a cath B inhibitor) but not pepstatin A (a cath D inhibitor) inhibited curcumin-induced cell apoptosis, mitochondrial membrane potential loss, and cytochrome c release. The antioxidant N-acetylcysteine and glutathione attenuated LMP, suggesting that lysosomal destabilization was dependent on the elevation of reactive oxygen species and which precedes mitochondrial dysfunction. These findings indicated a novel pathway for curcumin regulation of ROS-lysosomal-mitochondrial pathway and provided the key mechanism of regulation of LMP in cell apoptosis, which may be exploited for cancer treatment. © 2011 Springer Science+Business Media, LLC.


Chen Q.-Y.,Zhejiang University | Chen Q.-Y.,117Th Hospital of PLA | Jiao D.E.-M.,117Th Hospital of PLA | Yao Q.-H.,Zhejiang Chinese Medical University | And 5 more authors.
International Journal of Oncology | Year: 2012

Cdc42, a Rho GTPase family member, is involved in cell transformation, proliferation, survival, invasion and metastasis of human cancer cells. Overexpression of Cdc42 has been reported in several types of human cancer. However, the underlying mechanisms are not well understood. The present study showed that Cdc42 was overexpressed in 80 of 110 primary lung cancer patients, and overexpression of Cdc42 was significantly associated with high TNM stage and lymph node metastasis. Moreover, RNAi-mediated suppression of Cdc42 expression reduced actin formation, migration and invasion potential of a highly metastatic lung cancer cell line, 801D. In parallel, 801D cells were treated with curcumin and the effect on the expression of the Cdc42 gene at the transcriptional and translational levels was analyzed by RT-PCR and Western blotting. Curcumin inhibited cell migration, invasion and downregulated Cdc42 gene and Cdc42-related target gene expression in 801D cells. It also induced rearrangements of the actin cytoskeleton. These effects mimicked those of Cdc42 knockdown. Furthermore, xenograft experiments confirmed the suppression of tumor growth and invasion in vivo, which was due to the effect of curcumin and the inhibition of Cdc42 by curcumin. Our results showing the downregulation of Cdc42 expression by curcumin in lung cancer cells taken together with the clinical data suggest a potential therapeutic role for curcumin in inducing Cdc42-mediated inhibition of invasion of lung cancer cells.


Chen Q.-Y.,117th Hospital of PLA | Xu W.,Zhejiang Chinese Medical University | Jiao D.-M.,117th Hospital of PLA | Wu L.-J.,117th Hospital of PLA | And 3 more authors.
Molecular and Cellular Biochemistry | Year: 2013

Ezrin, primarily acts as a linker between the plasma membrane and the cytoskeleton, is involved in many cellular functions, including regulation of actin cytoskeleton, control of cell shape, adhesion, motility, and modulation of signaling pathways. Although ezrin is now recognized as a key component in tumor metastasis, its roles and the underlying mechanisms remain unclear. In the present study, we chose highly metastatic human lung carcinoma 95D cells, which highly express the ezrin proteins, as a model to examine the functional roles of ezrin in tumor suppression. An ezrin-silenced 95D cell line was established using lentivirus-mediated short hairpin RNA method. CCK-8 assay and soft agar assay analysis showed that downregulation of ezrin significantly suppressed the tumorigenicity and proliferation of 95D cells in vitro. cell migration and invasion studies showed that ezrin-specific deficiency in the cells caused the substantial reduction of the cell migration and invasion. In parallel, it also induced rearrangements of the actin cytoskeleton. Flow cytometry assay showed that changes in the ezrin protein level significantly affected the cell cycle distribution and eventual apoptosis. Furthermore, further studies showed that ezrin regulated the expression level of E-cadherin and CD44, which are key molecules involved in cell growth, migration, and invasion. Meanwhile, the suppression of ezrin expression also sensitized cells to antitumor drugs. Altogether, our results demonstrated that ezrin played an important role in the tumorigenicity and metastasis of lung cancer cells, which will benefit the development of therapeutic strategy for lung cancer. © 2013 Springer Science+Business Media New York.


You C.,Zhejiang University | Han C.,Zhejiang University | Wang X.,Zhejiang University | Zheng Y.,Zhejiang University | And 4 more authors.
Molecular Biology Reports | Year: 2012

Nanotechnology is a highly promising field, with nanoparticles produced and utilized in a wide range of commercial products. Silver nanoparticles (AgNPs) has been widely used in clothing, electronics, bio-sensing, the food industry, paints, sunscreens, cosmetics and medical devices, all of which increase human exposure and thus the potential risk related to their short- and long-term toxicity. Many studies indicate that AgNPs are toxic to human health. Interestingly, the majority of these studies focus on the interaction of the nano-silver particle with single cells, indicating that AgNPs have the potential to induce the genes associated with cell cycle progression, DNA damage and mitochondrial associated apoptosis. AgNPs administered through any method were subsequently detected in blood and were found to cause deposition in several organs. There are very few studies in rats and mice involving the in vivo bio-distribution and toxicity, organ accumulation and degradation, and the possible adverse effects and toxicity in vivo are only slowly being recognized. In the present review, we summarize the current data associated with the increased medical usage of nano-silver and its related nano-materials, compare the mechanism of antibiosis and discuss the proper application of nano-silver particles. © 2012 Springer Science+Business Media B.V.


Wang X.,Zhejiang University | You C.,Zhejiang University | Hu X.,Zhejiang University | Zheng Y.,Zhejiang University | And 6 more authors.
Acta Biomaterialia | Year: 2013

Full-thickness skin defects represent a significant and urgent clinical problem. Dermal substitutes serving as a regenerative template to induce dermal reconstruction provide a promising method to treat serious skin defects. Although collagen-chitosan dermal scaffolds display good biocompatibility and a suitable porous structure for angiogenesis and tissue regeneration, their poor mechanical properties compromise their application. To develop a well-supported dermal substitute, a poly(L-lactide-co-glycolide) (PLGA) knitted mesh was fabricated and integrated with collagen-chitosan scaffold (CCS) to obtain a PLGA knitted mesh-reinforced CCS (PLGAm/CCS). The morphology of this PLGAm/CCS was investigated in vitro. To characterize the tissue response, specifically angiogenesis and tissue regeneration, the PLGAm/CCS was transplanted in combination with thin split-thickness autografts to repair full-thickness skin wounds using a one-step surgical procedure in Sprague-Dawley rats. These results were then compared with CCSs. At weeks 2, 4 and 8 after the operation, the healing wounds were imaged to analyse wound changes, and tissue specimens were harvested for histology, immunohistochemistry, real-time quantitative polymerase chain reaction and Western blot analysis. The results demonstrated that collagen-chitosan sponge in the PLGAm/CCS remained porous, interconnected and occupied the openings of PLGA mesh, and the incorporation of the PLGA knitted mesh into CCS improved the mechanical strength with little influence on its mean pore size and porosity. Following transplantation, PLGAm/CCS inhibited wound contraction, and effectively promoted neotissue formation and blood vessel ingrowth. In conclusion, the mechanical strength of the scaffolds plays an important role in the process of tissue regeneration and vascularization. The ability of PLGAm/CCS to promote angiogenesis and induce in situ tissue regeneration demonstrates its potential in skin tissue engineering. © 2013 Acta Materialia Inc. Published by Elsevier Ltd.


Pan J.-H.,117th Hospital of PLA | Dong M.-J.,Zhejiang University | Ouyang X.-B.,117th Hospital of PLA
Clinical Nuclear Medicine | Year: 2011

Hepatoid adenocarcinoma is a rare variant of adenocarcinoma that can occur in the stomach. Because of the low incidence of the disease, F-18 fluorodeoxyglucose positron emission tomography with computed tomography (FDG PET/CT) imaging studies for hepatoid adenocarcinoma of the stomach are scarce. We report the case of a 79-year-old man presenting with hepatoid adenocarcinoma of the stomach associated with hepatic metastases mimicking hepatocellular carcinoma on FDG PET/CT images. The possible presence of metastatic hepatoid adenocarcinoma should be considered in older patients with elevated serum α-fetoprotein and hepatic tumors with underlying chronic liver disease. Copyright © 2011 by Lippincott Williams & Wilkins.


Chen Q.,117th Hospital of PLA | Jiao D.,117th Hospital of PLA | Hu H.,117th Hospital of PLA | Song J.,117th Hospital of PLA | And 3 more authors.
Oncology Research | Year: 2013

LIM kinase 1 (LIMK1) is a member of a novel class of serine-threonine protein kinases, which plays an important role in malignant transformation. High expression of LIM kinase 1 (LIMK1) has been detected in various invasive cancers. Here, we showed that LIMK1 was overexpressed in non-small cell lung cancer tissues (NSCLC) and cell lines. Expression of LIMK1 was detected in 115 of 150 lung cancer tissues, the frequency being more significant than in lung tissues. In addition, overexpression of LIMK1 was also associated with high TNM stage and lymph node metastasis in NSCLC patients. Moreover, RNAi-mediated suppression of LIMK1 expression markedly inhibited migration and invasion of 801D lung cancer cells. Furthermore, silencing of LIMK1 sensitized 801D cells to chemotherapeutic drugs of cisplatin and gemcitabine. These results indicate that the overexpression of LIMK1 is tightly associated with an aggressive phenotype of lung cancer cells, knockdown of LIMK1 suppressed cell migration and invasion, enhanced chemosensitivity, suggesting a potential therapeutic target for lung cancer. Copyright © 2013 Cognizant Comm. Corp.


Chen J.,117th Hospital of PLA | Song D.,117th Hospital of PLA
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2014

Background: The pathogenesis and management of human autoimmunity uveoretinitis is a focus in ophthalmology. For decades, a traditional experimental autoimmunity uveoretinitis (EAU) induced by pertussis toxin (PTX) was used for the basic investigation, which was thought to have a large deviation from the natural course of human autoimmunity uveoretinitis. Objective: This study was to establish a new mice model of autoimmunity uveoretinitis which mimics the human autoimmunity uveoretinitis pathogenesis and offer a basis for the investigation and treatment of uveoretinitis. Methods: Twenty 6-8 weeks old specific pathogen-free female C57BL/6(H-2) mice were randomized into normal control group, only endotoxin (lipopolysaccharide, LPS) induced uveitis group (endotoxin induced uveitis [EIU]group), interphotoreceptor retinoid-binding protein (IRBP1-20)+complete Freund adjuvant (CFA) induced uveoretinitis group (EAU group) and IRBP + CFA + LPS induced uveoretinitis group (LPS-EAU group). The mice of the EAU were only immunized with IRBP emulsified in CFA, and LPS-EAU group firstly were immunized with IRBP emulsified in CFA and then LPS was injected in the footpad of the mice on 7 days following immunization. The ocular pathological examination, histopathological scoring, delayed-type hypersensitivity and specific lymphocyte proliferating response were evaluated and compared with the EIU models, traditional EAU models without PTX and LPS-EAU models. The use and care of experimental animals complied with Regulation for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results: No inflammatory response was found in the iris, cilliary body and retina of mice in the normal control group. However, mild blood vessels dilation and fibrin exudation were seen in the iris and cilliary body of mice in the EIU group. In the EAU group, mild vasculitis and swelling of nerve fiber layer were exhibited in the retinas; while in the LPS-EAU group, severe disorder of retinal structure, infiltration of inflammatory cells and damage of photoreceptor were found under the optical microscope. The pathological score was 0 in the models of the normal control group and EIU group, 0.5 score in the EAU group and 3.0 scores in the LPS-EAU group, with a significant difference in the pathological scores between the EAU group and the LPS-EAU group (U=16.246, P=0.001). The earthickness of the mice was (35.60 ± 0.55) μm in the LPS-EAU group, and this value was significantly higher than (12.60 ± 0.55) μm of the EIU group (q=23.003, P<0.01), but closed to (34.80 ± 0.84) μm of the EAU group (t=0.820, P>0.05). The obvious cloning were seen and theradiation count per minute was (8540.00 ± 54.77)/min in the model mice of the LPS-EAU group, and that in the EAU group was (8484.00 ± 47.75)/min, without significant difference between them (q=56.634, P=0.069). Compared with the β particle number (2 050.00 ± 50.00)/min in the EIU group, that of the LPS-EAU group was significantly elevated (q=195.683, P=0.000). Conclusions: LPS injection can induce EAU in mice, and this model can better imitate the pathogenesis of human autoimmunity uveoretinitis. Copyright © 2014 by the Chinese Medical Association.


Chen Q.-Y.,117th Hospital of PLA
Chinese Journal of Oncology | Year: 2012

Objective: To explore the expression of ezrin protein in human non-small cell lung cancer (NSCLC) tissues and lung cancer cell lines, and the association between the expression of ezrin protein and the expression of E-cadherin and CD44V6 proteins. Methods: The expression of ezrin protein and mRNA in lung cancer cell lines was detected by RT-PCR and Western blotting. Ezrin, E-cadherin and CD44V6 were detected by immunohistochemical SP staining in tumor tissues from 150 lung cancer cases and in adjacent normal lung tissues from 30 patients. Furthermore, the expression of ezrin in 30 freshly-taken NSCLC tissues was also detected by Western blot. Results: The expression of ezrin protein and mRNA was up-regulated in highly metastatic human lung cancer. The positive rate of ezrin, E-cadherin and CD44V6 expression in the lung cancer was 61.3%, 54.0% and 58.7%, respectively. The up-regulation of ezrin expression was significantly correlated with lymph node metastasis, but not correlated with age, sex, tumor size, histological type, clinical TNM system and pathological grade. Western blot analysis showed that the level of ezrin in the NSCLC tissues was significantly higher than that in the normal tissues (t = 5.013, P < 0.01). Survival analysis showed that the 5-year survival rate of patients with negative ezrin expression was 29.3%, significantly higher than that of patients with positive ezrin expression (15.2%, χ2 = 4.128, P = 0.042). Multivariate Cox regression analysis showed that ezrin expression (RR = 3.012, P = 0.047) and lymph node metastasis (RR = 4.827, P = 0.035) were significantly independent prognostic factors for patients with lung cancer. Furthermore, a negative correlation was observed between the expressions of ezrin and E-cadherin in lung cancer, and a positive correlation between the expressions of ezrin and CD44V6 in lung cancer. Conclusions: Ezrin, E-cadherin and CD44V6 play an important role in the regulation of growth and meastasis of lung cancer. Combined detection of ezrin, E-cadherin and CD44V6 expression is helpful in evaluating the metastasis and prognosis of non-small cell lung cancer.

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