Wang C.,105th Hospital of PLA |
Yu G.,Shanghai University |
Liu J.,105th Hospital of PLA |
Wang J.,105th Hospital of PLA |
And 4 more authors.
Journal of Clinical Neuroscience | Year: 2012
Recent evidence has indicated that biological markers are essential in estimating the prognosis of patients with gliomas. The aim of this study was to determine the status and clinical significance of a novel tumor suppressor, PCDH9 (protocadherin 9) in glioma using tissue microarrays and immunohistochemistry. Normal brain tissue showed strong positive immunostaining for PCDH9, but this was downregulated in the primary cerebral glial tumor samples (51.7%). Loss of PCDH9 expression was associated significantly with a higher histological grade. Survival analysis demonstrated that patients with PCDH9-negative tumors had significantly shorter survival times than those with PCDH9-positive tumors and that PCDH9 was an independent prognostic factor. Our results suggest that PCDH9 might function as a tumor suppressor during cancer development and progression and could be regarded as a useful biomarker for predicting the outcome of patients with cerebral glial tumors. © 2011 Elsevier Ltd. All rights reserved.
Zhang Y.,Chongqing Medical University |
Tang J.,105th Hospital of PLA |
Tian Z.,Chongqing Medical University |
van Velkinburgh J.C.,Van Velkinburgh Initiative for Collaboratory BioMedical Research |
And 3 more authors.
International Reviews of Immunology | Year: 2015
Fibrosis is a consequence of chronic inflammation and the persistent accumulation of extracellular matrix, for which the cycle of tissue injury and repair becomes a predominant feature. Both the innate and adaptive immune systems play key roles in the progress of fibrosis. The recently identified subsets of innate lymphoid cells (ILCs), which are mainly localize to epithelial surfaces, have been characterized as regulators of chronic inflammation and tissue remodeling, representing a functional bridge between the innate and adaptive immunity. Moreover, recent research has implicated ILCs as potential contributing factors to several kinds of fibrosis diseases, such as hepatic fibrosis and pulmonary fibrosis. Here, we will summarize and discuss the key roles of ILCs and their related factors in fibrotic diseases and their potential for translation to the clinic. © 2015 Informa Healthcare USA, Inc.
Qin Y.,Shandong University of Technology |
Sun M.,Shandong University of Technology |
You L.,Shandong University of Technology |
Wei D.,Shandong University |
And 6 more authors.
Orphanet Journal of Rare Diseases | Year: 2012
Background: Premature ovarian failure (POF) is a complex and heterogeneous disorder that is influenced by multiple genetic components. Numerous candidate gene studies designed to identify POF susceptibility loci have been published, but most positive findings have not been confirmed in follow up studies. We sought to determine if sequence variants previously associated with age at natural menopause (AANM) or early menopause (EM) contribute as well to genetic susceptibility to POF. Methods. Our study was performed on 371 unrelated idiopathic women with POF and 800 women controls, all Chinese Han. Thirty six SNPs from previous genome-wide association studies (GWAS) responsible for AANM or EM and 3 additional SNPs in ESR1, and 2 additional SNPs in PTHB1 were tested using the Sequenom MassARRAY iPLEX platform for genotyping. Results: Three SNPs - rs2278493 in HK3, rs2234693 in ESR1 and rs12611091 in BRSK1 - showed nominally significant association with POF. Thus, a plausible relationship could exist between ESR1, BRSK1, HK3 and POF. Conclusions: This largest association study undertaken to determine correlation between POF and AANM/EM revealed three significant SNPs (rs2278493, rs2234693, and rs12611091). All are associated with not only AAWM and EM but also POF. Insights into shared genetic susceptibility between POF and AANM/EM will provide novel entry points for unraveling genetic mechanism involved in ovarian reserve and oocyte aging processes. © 2012 Qin et al.
Yu L.,Anhui University of Science and Technology |
Wang L.-H.,Anhui University of Science and Technology |
Hu Z.-T.,105th Hospital of PLA |
You Y.-Z.,Anhui University of Science and Technology |
And 2 more authors.
Polymer Chemistry | Year: 2015
Sequential Michael addition-based thiol-ene and free radical mediated thiol-ene reactions for preparing sequence-ordered polymers are reported for the first time. The thiols are produced in situ via the ring-opening of thiolactones, and they can readily react with the electron-deficient carbon-carbon double bond of allyl methacrylate via Michael addition-based thiol-ene, but they are unable to react with the electron-rich carbon-carbon double bond of allyl methacrylate without radicals, and intermediates with thiol and alkene units are formed. After the Michael addition thiol-ene and ring-opening reactions are complete, the thiol is activated by UV irradiation, enabling reaction with the electron-rich carbon-carbon double bond via a free radical-mediated thiol-ene reaction, to form sequence-ordered polymers of high molecular weights. This journal is © The Royal Society of Chemistry 2015.
Ding Z.,Wenzhou Medical College |
Ding Z.,Peoples Hospital of Yuyao |
Ou R.,Wenzhou Medical College |
Ni B.,Chongqing Medical University |
And 2 more authors.
Clinical and Vaccine Immunology | Year: 2013
Heat shock proteins (HSPs) have been successfully applied to a broad range of vaccines as biological adjuvants to enhance the immune response. The recently defined HSP110, in particular, exhibits strong protein binding affinity and is capable of enhancing the immunogenicity of protein antigens remarkably more than other HSP family members. In our previous study, we verified that murine HSP110 (mHSP110) significantly enhanced the immune response of a C57BL/6 mouse model to the H-2d-restricted human papillomavirus (HPV) E749-57 epitope (short peptide spanning the 49th to 57th amino acid residues in the E7 protein). To determine whether HSP110 similarly enhances the immunogenicity of human epitope peptides, we used the HLA-A2 transgenic mouse model to investigate the efficacy of the mHSP110 chaperone molecule as an immunoadjuvant of the human HLA-A2-restricted HPV16 E711-20 epitope vaccine. Results showed that mHSP110 efficiently formed a noncovalently bound complex with the E7 11-20 epitope. The mHSP110-E711-20 complex induced epitope-specific splenocyte proliferation and E711-20-specific gamma interferon (IFN-γ) secretion. Importantly, cytotoxic T lymphocytes primed by the mHSP110-E711-20 complex exerted strong cytolytic effects on target T2 cells pulsed with the E711-20 peptide or TC-1 cells transfected with the HLA-A2 gene. In addition, the mHSP110-E7 11-20 complex elicited stronger ex vivo and in vivo antitumor responses than either emulsified complete Freund's adjuvant or HSP70-chaperoned E711-20 peptide. These collective data suggest that HSP110 is a promising immunomodulator candidate for peptide-based human cancer vaccines, such as for the HLA-A2-restricted E711-20 epitope. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
Zhu Y.,105th Hospital of PLA |
Zhu Y.,Anhui Medical University |
Guan Y.-M.,105th Hospital of PLA |
Huang H.-L.,105th Hospital of PLA |
And 2 more authors.
Acta Pharmacologica Sinica | Year: 2014
Aim: Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) have been shown to ameliorate cerebral ischemia in animal models. In this study we investigated the effects of hUCB-MSCs on inflammatory responses and neuronal apoptosis during the early stage of focal cerebral ischemia in rabbits. Methods: Focal cerebral ischemia was induced in male New Zealand rabbits by occlusion of MCA for 2 h. The blood samples were collected at different time points prior and during MCAO-reperfusion. The animals were euthanized 3 d after MCAO, and the protein levels of IL-1β, IL-6, IL-10 and TNF-α in the serum and peri-ischemic brain tissues were detected using Western blot and ELISA, respectively. Inflammatory cell infiltration, neuronal apoptosis and neuronal density were measured morphologically. hUCB-MSCs (5×106) were iv injected a few minutes after MCAO. Results: The serum levels of IL-1β, IL-6 and TNF-α were rapidly increased, and peaked at 2 h after the start of MCAO. hUCB-MSC transplantation markedly and progressively suppressed the ischemia-induced increases of serum IL-1β, IL-6 and TNF-α levels within 6 h MCAO-reperfusion. Focal cerebral ischemia decreased the serum level of IL-10, which was prevented by hUCB-MSC transplantation. The expression of IL-1β, IL-6, IL-10 and TNF-α in the peri-ischemic brain tissues showed similar changes as in the serum. hUCB-MSC transplantation markedly suppressed the infiltration of inflammatory cells, and increased the neuronal density around the ischemic region. Furthermore, hUCB-MSC transplantation significantly decreased the percentage of apoptosis around the ischemic region. Conclusion: hUCB-MSCs transplantation suppresses inflammatory responses and neuronal apoptosis during the early stage focal cerebral ischemia in rabbits. © 2014 CPS and SIMM All rights reserved.
Han L.-F.,Anhui University of Science and Technology |
Chen Q.-B.,Anhui University of Science and Technology |
Hu Z.-T.,105th Hospital of PLA |
Piao J.-G.,Anhui University of Science and Technology |
And 3 more authors.
Macromolecular Rapid Communications | Year: 2014
A new and easy method of stimuli-triggered growth and removal of a bioreducible nanoshell on nanoparticles is reported. The results show that pH or temperature could induce the aggregation of disulfide-contained branched polymers at the surface of nanoparticles; subsequently, the aggregated polymers could undergo intermolecular disulfide exchange to cross-link the aggregated polymers, forming a bioreducible polymer shell around nanoparticles. When these nanoparticles with a polymer shell are treated with glutathione (GSH) or d,l-dithiothreitol (DTT), the polymer shell could be easily removed from the nanoparticles. The potential application of this method is demonstrated by easily growing and removing a bioreducible shell from liposomes, and improvement of in vivo gene transfection activity of liposomes with a bioreducible PEG shell. Temperature or pH triggers the growth of a bioreducible nanoshell onto silica nanoparticles, and reducing agent triggers the removal of the bioreducible nanoshell from silica nanoparticles. Removal of the core gives bioreducible nanocapsules. The nanoshell is cross-linked via disulfide bonds, which are responsive to the concentration of GSH, and so the polymer shell is bioreducible, and this method will have potential application in nanomedicine. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Hu F.,Anhui Medical University |
Lv D.,105th Hospital of PLA |
Zhu J.,105th Hospital of PLA |
Fang J.,105th Hospital of PLA
Traffic Injury Prevention | Year: 2014
Objective: To explore the related risk factors of injuries caused by e-bike and bicycle crashes in Hefei, Anhui. Methods: Between June 2009 and June 2011, the records of injuries were triggered by e-bike and bicycle crashes in Hefei maintained by 105th Hospital of PLA. A form was designed to document patient age, gender, road user category (driver, passenger, pedestrian), safety factors (safety devices present, speed, traffic violations), environmental factors (time of trauma, light conditions, road surface), crash mode, impact type, and vehicle type. Results: Of the 205 cases, 108 were female and 97 were male. One hundred forty-six patients suffered injuries due to e-bike accidents and 59 due to bicycle accident. The chi-squared test compared distribution of categorical variables suggested that age (P =.0250), road user category (P =.0278), traffic rule violations (P =.0132), crash mode (P =.0027), impact type (P =.0019), and vehicle type (P =.0219) are related to the severity of injuries caused by e-bike/bicycle crashes in Hefei. The multiple-factor nonconditional logistic regression analysis showed that injury severity is the most commonly sustained within the vehicle type (odds ratio [OR] = 14.418; 95% confidence interval [CI], 4.680-44.418), followed by crash mode (OR = 11.556; 95% CI, 4.430-30.142), traffic rule violations (OR = 4.735; 95% CI, 1.934-11.594), and age (OR = 2.910; 95% CI, 1.213-6.979). Conclusions: With the study of e-bike/bicycle crashes in Hefei, primary identification of the risk factors for the traffic injuries is obtained. These findings are important in decision making regarding preventive measures. © 2014 Copyright Taylor and Francis Group, LLC.
Lu D.-H.,105th Hospital of PLA |
Fei Z.-L.,105th Hospital of PLA |
Zhou J.-P.,105th Hospital of PLA |
Hu Z.-T.,105th Hospital of PLA |
Hao W.-S.,105th Hospital of PLA
Journal of Medical Imaging and Radiation Oncology | Year: 2015
Introduction We investigated the therapeutic effects of three-dimensional conformal radiotherapy combined with transcatheter arterial chemoembolisation (TACE) for hepatocellular carcinoma (HCC) with portal vein tumour thrombosis (PVTT). Methods Sixty-three HCC patients with PVTT were divided into two groups. Group A (30 patients) was treated with three-dimensional conformal radiotherapy followed by 2-3 series of TACE, while group B (33 patients) was only treated with TACE. Results The 1- and 2-year survival rates of group A were 62.40% and 20.81%, respectively, with a mean survival time of 13.0 months. The 1- and 2-year survival rates of group B were 56.49% and 18.83%, respectively, with a mean survival time of 9.0 months. There were significant differences between the two groups (log-rank chi-square value = 3.950, P = 0.047). Conclusion Three-dimensional conformal radiotherapy combined with TACE can significantly improve clinical outcomes in patients with HCC and PVTT compared with TACE alone. © 2014 The Royal Australian and New Zealand College of Radiologists.
Wang H.,Anhui Medical University |
Guan W.,105Th Hospital of PLA |
Yang W.,First Hospital of Anqing |
Wang Q.,Anhui Medical University |
And 4 more authors.
PLoS ONE | Year: 2014
Hepatic stellate cell (HSC) activation is an essential event during alcoholic liver fibrosis. Evidence suggests that adenosine aggravates liver fibrosis via the adenosine A2A receptor (A2AR). Caffeine, which is being widely consumed during daily life, inhibits the action of adenosine. In this study, we attempted to validate the hypothesis that caffeine influences acetaldehyde-induced HSC activation by acting on A2AR. Acetaldehyde at 50, 100, 200, and 400 μM significantly increased HSC-T6 cells proliferation, and cell proliferation reached a maximum at 48 h after exposure to 200 μM acetaldehyde. Caffeine and the A2AR antagonist ZM241385 decreased the cell viability and inhibited the expression of procollagen type I and type III in acetaldehyde-induced HSC-T6 cells. In addition, the inhibitory effect of caffeine on the expression of procollagen type I was regulated by A2AR-mediated signal pathway involving cAMP, PKA, SRC, and ERK1/2. Interestingly, caffeine's inhibitory effect on the expression of procollagen type III may depend upon the A2AR-mediated P38 MAPK-dependent pathway. Conclusions: Caffeine significantly inhibited acetaldehyde-induced HSC-T6 cells activation by distinct A2AR mediated signal pathway via inhibition of cAMP-PKA-SRC-ERK1/2 for procollagen type I and via P38 MAPK for procollagen type III. © 2014 Wang et al.