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Hefei, China

Li B.,Wuxi Second Peoples Hospital | Wang Y.,Fudan University | Li S.,Shanghai JiaoTong University | He H.,Shanghai University | And 5 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2015

microRNAs (miRNAs) are a class of small non-coding RNAs that play important roles in a variety of biological process. It has been reported that dysregulation of miRNA is always associated with cancer progression and development, and miR-378 aberrant expression has been found in some types of cancers. However, the association of miR-378 and glioma has not been evaluated. In this work, we measured the expression of miR-378 in glioma tissues and non-neoplastic brain tissues was measured using real-time PCR, and found that miRNA-378 expression level was significantly lower in glioma tissues compared with non-neoplastic brain tissues. Patients with lower miR-378 expression level had significantly poorer overall survival. Multivariate Cox regression analysis showed that miR-378 expression was an independent prognostic factor for 5-year overall survival. Over-expression of miR-378 inhibits glioma cell migration and invasion. In conclusion, our results indicated that miR-378 may serve as a tumor suppressor and play an important role in inhibiting tumor migration and invasion. Our work implicates the potential effect of miR-378 on the prognosis of glioma. Source


Tian R.-X.,105 Hospital of PLA | Chen X.,105 Hospital of PLA | Wang C.,105 Hospital of PLA | Pei H.,105 Hospital of PLA | And 2 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2013

Objective To measure the left ventricle global and segmental longitudinal strain, strain rate, and peak velocity in normal fetuses using velocity vector imaging (VVI) for the assessment of the left ventricular function. Methods Detailed prenatal echocardiographic examinations were performed in 53 normal fetuses. Digital dynamic four-chamber views were collected and analyzed offline. In the four-chamber view, the interventricular septum and left ventricular lateral wall were divided into basal, middle and apical segments, in a total of six segments. Endocardial tracing began at the edge of the anterior mitral valve annulus, along endocardium of interventricular septum, extended to the ventricular apex, and returned to posterior mitral valve annulus along lateral ventricular wall edge. The global and segmental longitudinal strain, strain rate and peak velocity of the interventricular septum and left ventricular lateral wall were measured, the global function was calculated by an average of all segments. Segmental measurements were compared to global results. Results Fifty-three fetuses were enrolled in this study, and the VVI analysis was successful in 49, the success rate was 92.4%. The global left ventricular strain was -13.72%±3.99%, and the strain rate was -2.59±0.44/s and 2.43±0.38/s in systole and diastole respectively. No difference was found between segmental and global ventricular strain and strain rate. The left global ventricular peak velocity was 1.68±0.46cm/s and 1.51±0.41cm/s in systole and diastole respectively. The peak segmental velocity in systole and diastole decreased from the base of interventricular septum and lateral wall to ventricular apex. The global left ventricular peak velocity was significantly different from the peak velocity of apical and basal segments (P<0.05). Conclusions Measurement of global left ventricular longitudinal strain, strain rate and peak velocity is feasible in fetuses by using VVI. Global left ventricular function may be a new index for assessment of quantitative fetal cardiac function. Source


Zhang D.,105 Hospital of PLA | Pei S.-B.,105 Hospital of PLA | Wang H.-F.,105 Hospital of PLA | Jiang C.-H.,105 Hospital of PLA | And 5 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2013

Objectives To explore the pathological changes in spinal cord injury with seawater immersion, and provide guidance for the treatment of such injury. Methods The rabbit model of spinal cord injury was reproduced by the modified Allen's method. Ninety-six rabbits were randomly divided into 4 groups: group A (normal control, n=6), in them only the vertebral plate was cut off but the spinal cord was not injured; group B (n=30), vertebral plate was resected and the spinal cord was damaged without sea water immersion; group C (n=30), the vertebral plate was resected and the spinal cord was damaged and treated with normal saline; and group D (n=30), the vertebral plate was resected and the spinal cord was damaged followed by seawater immersion. The immersion time was 60 minutes. In group B, C and D, 6 rabbits were randomly selected from each group at 1, 6, 12, 24 and 48 hours after immersion, and the neurological function of rabbits were evaluated using the Tarlov scale, then the animals were sacrificed, and the histopathological features were studied with light microscopy, the expressions of Bax and Bcl-2 were determined by immunohistochemistry, and the apoptosis of neurons in spinal cord was evaluated by TUNEL method. Results Compared with group A, the Tarlov scores of group B, C and D decreased significantly, but no significant difference was found between the 3 groups at 1h after treatment, while the Tarlov scores were higher at 6h and lower at 12h in group D as compared with those in group B and C (P<0.05). Edema of spinal cord was observed in group B, C and D after injury. Distinct traumatic spinal cord edema was found at 6h in group B and C and at 12h in group D, but no significant difference was observed between group B and C. Compared with group B and C, the spinal cord edema in group D was less distinct at 6h and more serious at 12h. The spinal cord edema and hyperemia were alleviated in all 3 groups at 24h and 48h. Positive expression of Bcl-2 and Bax was found at each time point after injury in group B, C and D. Compared with group B and C, the expression of Bax decreased at 6h and increased at 12, 24 and 48h in group D, while the expression of Bcl-2 decreased at 6, 12, 24 and 48h in group D (P<0.05). The number of apoptotic cells increased significantly in group B, C and D, and all of them reached peak at 12h. Compared with group B and C, the number of apoptotic cells increased at 12h and decreased at 6, 24 and 48h in group D (P<0.01). Conclusion In early stage, seawater immersion may inhibit the spinal cord injury, delay the pathological changes in traumatic spinal cord injury and neuron apoptosis, but may aggravate spinal cord injury in later stage. Source

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