101 Research Institute of the Ministry of Civil Affairs

Beijing, China

101 Research Institute of the Ministry of Civil Affairs

Beijing, China
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Han L.,Guangdong Institute of Eco environmental and Soil Sciences | Han L.,Sichuan Normal University | Yang G.Q.,Guangdong Institute of Eco environmental and Soil Sciences | Zhou X.,101 Research Institute of the Ministry of Civil Affairs | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A Gram-reaction-positive, facultatively anaerobic, motile, endospore-forming, rod-shaped strain, designated SgZ-7T, was isolated from a windrow compost pile and was characterized by means of a polyphasic approach. Growth occurred with 0-3 % (w/v) NaCl (optimum 1 %), at pH 6.0-10.0 (optimum pH 7.2) and at 40-60 °C (optimum 50 °C). The main respiratory quinone was MK-7. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were iso-C15: 0 and anteiso-C15: 0. The DNA G+C content was 46.6 mol%. The phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain SgZ-7T should be assigned to the genus Bacillus and was related most closely to Bacillus drentensis LMG 21831T (sequence similarity 97.2 %). The result of the DNA-DNA hybridization experiment revealed a low relatedness (27.2 %) between the isolate and B. drentensis LMG 21831T. The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain SgZ-7T represents a novel species, for which the name Bacillus thermocopriae sp. nov. is proposed. The type strain is SgZ-7T (= CCTCC AB 2012030T = KACC 16700T). © 2013 IUMS.


Yang G.,Guangdong Institute of Eco environmental and Soil Sciences | Zhou X.,101 Research Institute of the Ministry of Civil Affairs | Zhou S.,Guangdong Institute of Eco environmental and Soil Sciences | Yang D.,101 Research Institute of the Ministry of Civil Affairs | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A novel thermotolerant bacterium, designated SgZ-8T, was isolated from a compost sample. Cells were non-motile, endospore-forming, Gram-staining positive, oxidase-negative and catalasepositive. The isolate was able to grow at 20-65 °C (optimum 50 °C) and pH 6.0-9.0 (optimum 6.5-7.0), and tolerate up to 9.0% NaCl (w/v) under aerobic conditions. Anaerobic growth occurred with anthraquinone-2,6-disulphonate (AQDS), fumarate and NO3 - as electron acceptors. Phylogenetic analysis based on the16S rRNA and gyrB genes grouped strain SgZ-8T into the genus Bacillus, with the highest similarity to Bacillus badius JCM 12228T (96.2% for 16S rRNA gene sequence and 83.5% for gyrB gene sequence) among all recognized species in the genus Bacillus. The G+C content of the genomic DNA was 49.3 mol%. The major isoprenoid quinone was menaquinone 7 (MK-7) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major cellular fatty acid was iso-C16: 0. On the basis of its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-8T (=CCTCC AB 2012108T=KACC 16706T) was designated the type strain of a novel species of the genus Bacillus, for which the name Bacillus thermotolerans sp. nov. is proposed. © 2013 IUMS.


Yu Z.,Guangdong Institute of Eco environmental and Soil Sciences | Zhou X.,101 Research Institute of the Ministry of Civil Affairs | Wang Y.,Guangdong Institute of Eco environmental and Soil Sciences | Yang G.,Guangdong Institute of Eco environmental and Soil Sciences | Zhou S.,Guangdong Institute of Eco environmental and Soil Sciences
Biotechnology and Bioprocess Engineering | Year: 2015

Dye wastewater normally is discharged at high temperature, but thermophilic bacteria capable of decolorizing azo dyes have rarely been isolated. Here we report a newly isolated moderately thermophilic bacterium, Novibacillus thermophilus SG-1, which had a remarkable ability to decolorize the azo dye Orange I by utilizing a large variety of organic substrates as electron donors. When Orange I served as the sole electron acceptor, almost complete decolorization occurred at 50ºC and pH 8.0 with acetate as the electron donor after anaerobic incubation of strain SG-1 for 24 h. The decolorization process followed the pseudofirst- order kinetics. The complete reduction of 0.3 mM Orange I was accompanied by a stoichiometric consumption of 0.17 mM acetate over time. The measured molar ratio (1.76) of Orange I reduced to acetate oxidized was close to the theory value of 2.0, suggesting that most of the electrons released by acetate had been transported to Orange I. Simultaneously energy generated from the electron transfer process was used to support cell anaerobic growth, which meant that azoreduction by strain SG-1 is an azorespiration process. To our knowledge, this is the first report of a thermophilic bacterium capable of azorespiration, which increases the limited number of bacteria for treating hightemperature azo dye wastewater. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.

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