101 Hospital of PLA

Wuxi, China

101 Hospital of PLA

Wuxi, China
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Zong G.-J.,101 Hospital of PLA | Bai Y.,Shanghai University | Wu G.-Y.,101 Hospital of PLA | Jiang H.-B.,Shanghai University | Qin Y.-W.,Shanghai University
Circulation Journal | Year: 2011

Background: In recent years, some experimental and clinical studies on transcatheter aortic valve implantation (TAVI) have been conducted. TAVI is indicated in patients with calcified pure or predominant aortic stenosis. The risk of this technique is still high. Aortic valved stent implantation above the coronary ostia might avoid blocking the coronary ostia. Methods and Results: Twenty healthy dogs were selected to establish a canine model of acute aortic valve rupture. The dogs were randomly divided into 2 groups: the rupture model group without any treatment and the valved stent group with percutaneous valved stent implantation above the coronary ostia. The 2 groups of animals were followed up for 3 months. Echocardiography and other tests were performed to assess aortic regurgitation and ventricular function. Acute aortic valve rupture models were successfully established in 16 of 20 dogs. In the rupture model group, the mean aortic regurgitation was 6.8±1.9 ml/s; only 3 of 8 animals survived for 3 months. In the valved stent group, the mean aortic regurgitation was 7.0±2.1 ml/s; valved stents were successfully implanted in 8 animals. Instant post-implantation anatomy showed that the stents were located appropriately. Seven dogs survived for 3 months. Conclusions: Percutaneous valved stent implantation above the coronary ostia is feasible and effective as a transitional treatment for acute aortic valve rupture.


Zong G.-J.,101 Hospital of PLA | Bai Y.,Shanghai University | Gu M.-B.,Shanghai University | Jiang H.-B.,Shanghai University | And 3 more authors.
Journal of Thoracic and Cardiovascular Surgery | Year: 2011

Objective: The purpose of this study was to investigate the feasibility and safety of pulmonary valve implantation via direct right ventricle puncture. Methods: A standard thoracotomy and direct right ventricle puncture were performed in 8 healthy sheep to implant the pulmonary valve stents. Animals were followed up for 18 months. Results: Three sheep died within the first 4 months after stent placement. The remaining 5 animals survived. After 18 months, examinations by color echocardiography, 64-slice computed tomography scan, and cardiac catheter showed an ideal position of each stent. The function of the pulmonary valves and hearts was not different compared with the preoperative conditions of the sheep. Anatomic examination revealed that the stent was covered by a layer of endothelial tissue with no stent fracture or valvular calcification. The histologic evaluation of the stent and surrounding tissue showed that the surface of the stent was smooth and covered by a complete layer of endothelial cells without obvious infiltration of inflammatory cells. The vascular wall was integrative without tear phenomenon in each layer of tissue. Conclusions: These results show that pulmonary valve stents can be implanted via direct right ventricle puncture. Further studies evaluating xenograft valve material and the effect of implantation in vivo are needed. Copyright © 2011 by The American Association for Thoracic Surgery.


Mo X.,101 Hospital of PLA | Wang L.,Nanjing Southeast University | Zheng Q.,Nanjing Southeast University | Wu G.,101 Hospital of PLA
Journal of Nanoscience and Nanotechnology | Year: 2016

Circulating tumor cells (CTCs) have become established indicators for cancer therapy and prognosis. To effectively and specifically sort CTCs, numerous techniques have been developed in the last few decades. Recently, aptamer grafted nanostructured substrates have shown ultrasensitivity and considerable specificity in isolation of CTCs. However, due to the limitation of existing nanofabrication methods, large-scale fabrication of homogeneous nanostructured substrates is non-trivial and impose high fabrication costs. On the other hand, the heterogeneity of CTCs in biomarker expression also makes their isolation quite challenging. In this research, homogenous nanostructured surface on borosilicate glass substrates with more than 80 cm2 surface areas are successfully prepared at one time. Such area can process approximately 8 mL of blood. Bi-specific aptamers targeting epithelial cell adhesion molecule and prostate specific membrane antigen, respectively, are also tested. However, due to formation of hetero-dimers between two different aptamers originating from inherent annealing property of nucleic acids, bi-specific aptamers fail to efficiently capture cancer cells. This limitation reveals that multi-specific aptamers, when applied to cell isolation, must be analyzed to exclude any potential formation of hetero-dimers due to complementary sequence matching. Copyright © 2016 American Scientific Publishers All rights reserved.


Lu S.,PLA Fourth Military Medical University | Li T.,Second Artillery General Hospital of PLA | Li T.,PLA Fourth Military Medical University | Zhang Y.,PLA Fourth Military Medical University | And 4 more authors.
Computers in Biology and Medicine | Year: 2013

Three-dimensional (3D) finite element models of a posterior mandibular segment and a distraction implant (DI) were created and assembled in this study. The diameter of distraction screw (DS) was set as input variable, ranging from 1.0 to 3.0. mm, to analyze the stress and displacement, in order to obtain an optimal outcome of the DI. The results indicate that when the diameter of DS was 2.0. mm, the stresses in jaw bone and DS and the displacement of DS reached a relatively low range. The diameter of DS significantly affects the stress distribution in DI and surrounding bone. The present study demonstrates that from clinical and biomechanical points of view, the optimal diameter of DS is 2.0. mm. © 2013 Elsevier Ltd.


Lu W.,PLA Fourth Military Medical University | Lu W.,101 Hospital of PLA | Feng F.,PLA Fourth Military Medical University | Xu J.,PLA Fourth Military Medical University | And 9 more authors.
Cancer Biology and Therapy | Year: 2014

Cancer stem cells (CSCs) may contribute to tumor initiation, distant metastasis and chemo-resistance. One of RNA-binding proteins, Quaking (QKI), was reported to be a tumor suppressor. Here we showed that reduced QKI levels were observed in many human oral cancer samples. Moreover further reduction of QKI expression in CSCs was detected compared with non-CSCs in oral cancer cell lines. Overexpressing QKI in oral cancer cells significantly reduced CSC sphere formation and stem cell-associated genes. In tumor implanting nude mice model, QKI significantly impeded tumor initiation rates, tumor sizes and lung metastasis rates. As a contrast, knocking down QKI enhanced the above effects. Among the putative CSC target genes, SOX2 expression was negatively affected by QKI, mechanism study revealed that QKI may directly regulate SOX2 expression via specific binding with its 3′UTR in a cis element-dependent way. Loss of SOX2 even completely reversed the sphere forming ability in QKI knockdown cell line. Taken together, these data demonstrated that SOX2 is an important CSC regulator in oral cancer. QKI is a novel CSC inhibitor and impaired multiple oral CSC properties via partial repression of SOX2. Therefore, reduced expression of QKI may provide a novel diagnostic marker for oral cancer. © 2014 Landes Bioscience.


Differences in pathologic diagnosis between endoscopic forceps biopsy (EFB) and endoscopic submucosal dissection (ESD) for gastric intraepithelial neoplasia (GIN) and early gastric carcinoma (EGC) in Chinese patients remain unknown. The aim of the study was to investigate risk factors for under-diagnosed pathology in initial EFB, compared to final ESD. We reviewed endoscopic and histopathologic findings for tumor location, size, macroscopic pattern, nodularity, erythema, erosion, GIN (low and high grade), and EGC diagnosed with the WHO criteria. Differences in those features between EFB and ESD were compared and risk factors for under-diagnosis by EFB were analyzed. Although concordant in most (74.9%) cases between EFBs and ESDs, pathological diagnoses in 57 (25.1%) cases were upgraded in ESDs. Compared to the concordant group, the lesion size 2cm, and depressed and excavated patterns were significantly more frequent in the upgraded group. Further multivariate regression analysis demonstrated the depressed pattern and lesion size 2cm as independent risk factors for upgraded pathology with the odds ratio of 5.778 (95% confidence interval 2.893-11.542) and 2.535 (95% confidence interval 1.257-5.111), respectively. Lesion size 2.0cm and the depressed pattern at initial EFB were independent risk factors for pathologic upgrade to advanced diseases in ESD. Therefore, these endoscopic characteristics should be considered together with the initial EFB diagnosis to guide the optimal clinical management of patients with GIN and EGC.


Zhou Z.H.,101 Hospital of PLA
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2013

To investigate the sensitivity of gastric cancer stem cells (GCSCs) to 5-fluorouracil (5-FU), and to explore the cytological mechanism of drug-resistance of gastric cancer. Immunohistochemical staining was employed to examine expression of stem cell marker CD44 and drug-resistance protein thymidylate synthase (TS) in 71 cases with gastric cancer. Based on morphology of cell colonies derived from AGS gastric cell line, colonies consisting of GCSCs were isolated, and expression of CD44 and TS, as well as self-renewal capacity of GCSCs were detected. Sensitivity of GCSCs to 5-FU was examined through CCK-8 assay. The positive rates of CD44 and TS were 59.2% (42/71) and 56.3% (40/71) in gastric cancer, and expression of CD44 was associated with that of TS (χ(2)=12.76, P<0.01; Kappa=0.41). Serial sections indicated that CD44+ cancer cells simultaneously expressed TS. AGS developed morphologically diverse colonies, and the GCSCs colonies exhibited a tight and regular shape, which were called holoclone. Holoclones expressed CD44 and TS strongly, possessing capacity of robust self-renewal and forming a lot of second passage clones after incubation. Subclones expressed CD44 and TS weakly, forming less second passage clones. Paraclones almost did not express CD44 and TS, forming no second passage clone after incubation. Affected by 5-Fu, three holoclones showed less growth inhibition compared with another colony type and wild-type AGS cells. Furthermore, IC50 of 5-FU for three holoclones was (113.43±5.81), (272.68±25.75) and (118.14±17.75) μmol/L respectively, which were significantly higher than that of one subcolony type [(16.97±1.01) μmol/L] and AGS cells [(27.52±0.59) μmol/L] (all P<0.05). GCSCs possess lower sensitivity to 5-FU, and may play a critical role in drug-resistance of gastric cancer.


PubMed | 101 Hospital of PLA
Type: Journal Article | Journal: The Journal of surgical research | Year: 2013

We investigated the effects of percutaneous valved stent implantation in the ascending aorta as an alternative treatment for aortic regurgitation in a canine model.A total of 16 healthy dogs weighing an average of 18.3 2.1kg were used for the establishment of animal models of chronic aortic regurgitation by percutaneous aortic valve perforation and balloon dilation. At 2mo after successful model establishment, all experimental animals underwent valved stent implantation in the ascending aorta and then were followed up for 3mo.Experimental models of chronic aortic regurgitation were successfully established in 10 dogs. Surviving dogs underwent successful valved stent implantation in the ascending aorta and were subsequently followed up for 3mo. The level of instantaneous aortic regurgitation at 3-mo follow-up was significantly reduced compared with that before valved stent implantation (2.4 0.9 versus 10.62.1mL/s, P < 0.05). The left ventricular ejection fraction was significantly increased (53.8 4.2% versus 37.83.7%, P < 0.05), and the left ventricular end-diastolic volume was also significantly reduced (30.3 2.2 versus 40.13.6mL, P < 0.05). No paravalvular leak, stroke, atrioventricular block, or other complications occurred in dogs undergoing valved stent implantation.Percutaneous valved stent implantation in the ascending aorta is feasible, effective, and safe as an alternative treatment for very high-risk aortic regurgitation in a canine model.


PubMed | Anhui Medical University, Peoples Hospital of Jiangsu Province and 101 Hospital of PLA
Type: | Journal: Journal of inflammation (London, England) | Year: 2015

Obesity is regarded as a risk factor for cardiovascular disease. Bone morphogenetic protein 4 (BMP4) is a proinflammatory and profibrotic factor, and the reduced expression of this molecule in obese mice seems to be inconsistent with the known proinflammatory effects of obesity. Therefore, we studied BMP4 expression and inflammation in the myocardial tissue and aortas of obese mice.Four-week-old ob/ob mice were used as the experimental group, and C57BL/6 mice comprised the control group. Animals were sacrificed after a 12-week full diet, and then the blood, heart, abdominal aorta, and inguinal adipose tissue were collected. The expression of BMP4 mRNA and protein in the heart and aorta was significantly higher in the experimental group than in the control group, but expression was lower in adipose tissue. Inflammation measured by the expression of IL-1 and IL-9 mRNA and protein and Smad1 and phosphorylated Smad1/5/8 protein in the heart and aorta was higher in the experimental group than in the control group. In addition, the expression of BMP4 in the serum was significantly higher in the experimental group than in the control group.BMP4 is significantly overexpressed in the myocardial tissue and aortas of obese mice, and mediates local inflammatory responses.


PubMed | 101 Hospital of PLA
Type: Journal Article | Journal: Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2013

To investigate the sensitivity of gastric cancer stem cells (GCSCs) to 5-fluorouracil (5-FU), and to explore the cytological mechanism of drug-resistance of gastric cancer.Immunohistochemical staining was employed to examine expression of stem cell marker CD44 and drug-resistance protein thymidylate synthase (TS) in 71 cases with gastric cancer. Based on morphology of cell colonies derived from AGS gastric cell line, colonies consisting of GCSCs were isolated, and expression of CD44 and TS, as well as self-renewal capacity of GCSCs were detected. Sensitivity of GCSCs to 5-FU was examined through CCK-8 assay.The positive rates of CD44 and TS were 59.2% (42/71) and 56.3% (40/71) in gastric cancer, and expression of CD44 was associated with that of TS ((2)=12.76, P<0.01; Kappa=0.41). Serial sections indicated that CD44+ cancer cells simultaneously expressed TS. AGS developed morphologically diverse colonies, and the GCSCs colonies exhibited a tight and regular shape, which were called holoclone. Holoclones expressed CD44 and TS strongly, possessing capacity of robust self-renewal and forming a lot of second passage clones after incubation. Subclones expressed CD44 and TS weakly, forming less second passage clones. Paraclones almost did not express CD44 and TS, forming no second passage clone after incubation. Affected by 5-Fu, three holoclones showed less growth inhibition compared with another colony type and wild-type AGS cells. Furthermore, IC50 of 5-FU for three holoclones was (113.435.81), (272.6825.75) and (118.1417.75) mol/L respectively, which were significantly higher than that of one subcolony type [(16.971.01) mol/L] and AGS cells [(27.520.59) mol/L] (all P<0.05).GCSCs possess lower sensitivity to 5-FU, and may play a critical role in drug-resistance of gastric cancer.

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