Janakpuri, India
Janakpuri, India

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Dey Y.N.,1 65 institutional area
International Journal of Pharma and Bio Sciences | Year: 2011

Methanolic extract of the leaves of Paederia foetida were screened for its anthelmintic activity against Pheretima posthuma and Tubifex tubifex. The parameters like the time of paralysis and the time of death were determined by using the extract at the concentrations of 25,50 and 100 mg/ml. The extract exhibited significant anthelmintic activity at highest concentration of 100 mg/ml as compared with piperazine citrate (10 mg/ml) as standard reference and distilled water as control.


Saha S.,Bose Institute of India | Bhattacharjee P.,Bose Institute of India | Guha D.,Bose Institute of India | Kajal K.,Bose Institute of India | And 10 more authors.
International Journal of Oncology | Year: 2015

Adverse side effects of chemotherapy during cancer treatment have shifted considerable focus towards therapies that are not only targeted but are also devoid of toxic side effects. We evaluated the antitumorigenic activity of sulphur, and delineated the molecular mechanisms underlying sulphurinduced apoptosis in non-small cell lung carcinoma (NSCLC) cells. A search for the underlying mechanism revealed that the choice between the two cellular processes, NFκBp65-mediated survival and p53-mediated apoptosis, was decided by the competition for a limited pool of transcriptional coactivator protein p300 in NSCLC cells. In contrast, sulphur inhibited otherwise upregulated survival signaling in NSCLC cells by perturbing the nuclear translocation of p65NFκB, its association with p300 histone acetylase, and subsequent transcription of Bcl-2. Under such anti-survival condition, induction of p53-p300 cross-talk enhanced the transcriptional activity of p53 and intrinsic mitochondrial death cascade. Overall, the findings of this preclinical study clearly delineated the molecular mechanism underlying the apoptogenic effect of the non-toxic homeopathic remedy, sulphur, in NSCLC cells.


Saha S.,Bose Institute of India | Bhattacharjee P.,Bose Institute of India | Mukherjee S.,Bose Institute of India | Mazumdar M.,Bose Institute of India | And 7 more authors.
Oncology Reports | Year: 2014

The adverse side-effects associated with chemotherapy during cancer treatment have shifted considerable focus towards therapies that are targeted but devoid of toxic side-effects. In the present study, the antitumorigenic activity of thuja, the bioactive derivative of the medicinal plant Thuja occidentalis, was evaluated, and the molecular mechanisms underlying thuja-induced apoptosis of functional p53-expressing mammary epithelial carcinoma cells were elucidated. Our results showed that thuja successfully induced apoptosis in functional p53-expressing mammary epithelial carcinoma cells. Abrogation of intracellular reactive oxygen species (ROS), prevention of p53-activation, knockdown of p53 or inhibition of its functional activity significantly abridged ROS generation. Notably, under these conditions, thuja-induced breast cancer cell apoptosis was reduced, thereby validating the existence of an ROS-p53 feedback loop. Elucidating this feedback loop revealed bi-phasic ROS generation as a key mediator of thuja-induced apoptosis. The first phase of ROS was instrumental in ensuring activation of p53 via p38MAPK and its nuclear translocation for transactivation of Bax, which induced a second phase of mitochondrial ROS to construct the ROS-p53 feedback loop. Such molecular crosstalk induced mitochondrial changes i) to maintain and amplify the thuja signal in a positive self-regulatory feedback manner; and ii) to promote the mitochondrial death cascade through cytochrome c release and caspase-driven apoptosis. These results open the horizon for developing a targeted therapy by modulating the redox status of functional p53-expressing mammary epithelial carcinoma cells by thuja.


PubMed | Bose Institute of India, Bholanath Chakrabarty Trust and 1 65 Institutional Area
Type: Journal Article | Journal: International journal of oncology | Year: 2015

Adverse side effects of chemotherapy during cancer treatment have shifted considerable focus towards therapies that are not only targeted but are also devoid of toxic side effects. We evaluated the antitumorigenic activity of sulphur, and delineated the molecular mechanisms underlying sulphur-induced apoptosis in non-small cell lung carcinoma (NSCLC) cells. A search for the underlying mechanism revealed that the choice between the two cellular processes, NFBp65-mediated survival and p53-mediated apoptosis, was decided by the competition for a limited pool of transcriptional coactivator protein p300 in NSCLC cells. In contrast, sulphur inhibited otherwise upregulated survival signaling in NSCLC cells by perturbing the nuclear translocation of p65NFB, its association with p300 histone acetylase, and subsequent transcription of Bcl-2. Under such anti-survival condition, induction of p53-p300 cross-talk enhanced the transcriptional activity of p53 and intrinsic mitochondrial death cascade. Overall, the findings of this preclinical study clearly delineated the molecular mechanism underlying the apoptogenic effect of the non-toxic homeopathic remedy, sulphur, in NSCLC cells.


Siddiqui A.,Jamia Hamdard University | Tajuddin,Aligarh Muslim University | Amin K.M.Y.,Aligarh Muslim University | Zuberi R.H.,Aligarh Muslim University | Jamal A.,1 65 Institutional area
Indian Journal of Traditional Knowledge | Year: 2011

Standardization and quality control of the herbal drugs including single and compound formulations used in Unani System of Medicine is essential for their acceptance in the international as well as local markets. Thus, there is an urgent need of standardized drugs having consistent quality for reliable beneficial therapeutic purpose. An attempt has been made for phytochemical standardization of Majith (Rubia cordifolia Linn.) (Rubiaceae), a Unani drug having immunomodulatory, antioxidant, antiinflammatory, antipsychotic, antidiarrhoeal, anticonvulsant and antidiabetic properties. Physicochemical parameters, qualitative and quantitative analysis, TLC profile and fluorescence analysis were used for standardization and quality evaluation of Majith. Qualitative analysis of Majith showed the presence of amino acid, proteins, glycosides, flavanoids, phenols, resin, sterol/ terpene and tannins except alkaloids.

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